OBJECTIVE Recently,some reports show that curcuma has a good curative effect on the treatment of psoriasis;while curcuma has many components,so the present study was designed to investigate the effective parts of curc...OBJECTIVE Recently,some reports show that curcuma has a good curative effect on the treatment of psoriasis;while curcuma has many components,so the present study was designed to investigate the effective parts of curcuma on treatment of psoriasis,in addition,we will explore the mechanisms of curcuma therapeutic effect.METHODS First,we observed that curcuma′s extractions effect on mitosis of mouse vaginal epithelial cells;then psoriasis like was induced by wiping imiquimod in the surface of the binaural dorsal skin of the animal,the score of skin damage was measured on days 7 and 14;in order to explore the mechanisms of curcuma’s extractions on psoriasis,immune factors expression(CK14,CK16,CK17,PCNA,TLR-2,TLR-4,TLR-9)was determined in propranolol induced psoriasis like.RESULTS Curcuma′s extraction prohibited the mitosis of mouse vaginal epithelial cells;curcuma’s extractions produced a significant and dose dependent inhibition of psoriasis during the entire duration of the study in imiquimod induced psoriasis like;and the expression of immune factors(CK14,CK16,CK17,PCNA,TLR-2,TLR-4,TLR-9)was also found to be less in the curcuma′s extraction treated group as compared to control.CONCLUSION We believe that the curative effectof curcuma’sextraction may due to its inhibition effect on the expression of immune factors.Our results contribute towards validation of curcuma in the treatment of psoriasis and other joint disorders.展开更多
广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)为姜科姜黄属植物.通过抑菌实验和电镜技术等方法测定研究了广西莪术油对6种植物病原真菌的抑制作用,结果表明:广西莪术油具有较强的抗真菌活性,对植物病原真菌孢子萌发和菌丝生长...广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)为姜科姜黄属植物.通过抑菌实验和电镜技术等方法测定研究了广西莪术油对6种植物病原真菌的抑制作用,结果表明:广西莪术油具有较强的抗真菌活性,对植物病原真菌孢子萌发和菌丝生长均有显著的抑制作用.最低抑制浓度(MIC)的莪术油6.25mL/L对植物病原真菌孢子萌发和菌丝生长均有显著的抑制作用.半抑制率IC50表明广西莪术油对松赤枯病菌的抑制作用最强,最弱的是小麦赤霉病菌.电镜下观察菌丝的横切面表明,处理菌丝的细胞壁消失,原生质解体.研究结果表明广西莪术具有开发为生物农药的潜力.展开更多
目的探究广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)乙酸乙酯部位的抗血栓作用。方法建立角叉菜胶诱导小鼠尾血栓模型、大鼠动静脉旁路血栓模型、FeCl_3诱导大鼠体内血栓模型,观察该部位抗血栓形成的作用,ELISA法检测大鼠血...目的探究广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)乙酸乙酯部位的抗血栓作用。方法建立角叉菜胶诱导小鼠尾血栓模型、大鼠动静脉旁路血栓模型、FeCl_3诱导大鼠体内血栓模型,观察该部位抗血栓形成的作用,ELISA法检测大鼠血清中ET-1、6-keto-PGF_(1α)、TXB2水平,硝酸还原酶法测定NO水平。再建立大鼠急性血瘀模型,观察该部位对血液流变学的影响。结果广西莪术乙酸乙酯部位可显著减少角叉菜胶诱导小鼠黑尾血栓数量和黑尾长度;大鼠动静脉旁路血栓模型、FeCl_3诱导体内血栓模型的血栓湿重显著降低,血清中NO、6-keto-PGF_(1α)水平增加,ET-1、TXB2水平降低;大鼠急性血瘀模型的全血黏度、血浆黏度显著降低。结论广西莪术乙酸乙酯部位具有显著的抗血栓形成作用,其机制可能与提高NO、6-keto-PGF_(1α)水平,减少ET-1、TXB2水平,降低全血黏度、血浆黏度有关。展开更多
文摘OBJECTIVE Recently,some reports show that curcuma has a good curative effect on the treatment of psoriasis;while curcuma has many components,so the present study was designed to investigate the effective parts of curcuma on treatment of psoriasis,in addition,we will explore the mechanisms of curcuma therapeutic effect.METHODS First,we observed that curcuma′s extractions effect on mitosis of mouse vaginal epithelial cells;then psoriasis like was induced by wiping imiquimod in the surface of the binaural dorsal skin of the animal,the score of skin damage was measured on days 7 and 14;in order to explore the mechanisms of curcuma’s extractions on psoriasis,immune factors expression(CK14,CK16,CK17,PCNA,TLR-2,TLR-4,TLR-9)was determined in propranolol induced psoriasis like.RESULTS Curcuma′s extraction prohibited the mitosis of mouse vaginal epithelial cells;curcuma’s extractions produced a significant and dose dependent inhibition of psoriasis during the entire duration of the study in imiquimod induced psoriasis like;and the expression of immune factors(CK14,CK16,CK17,PCNA,TLR-2,TLR-4,TLR-9)was also found to be less in the curcuma′s extraction treated group as compared to control.CONCLUSION We believe that the curative effectof curcuma’sextraction may due to its inhibition effect on the expression of immune factors.Our results contribute towards validation of curcuma in the treatment of psoriasis and other joint disorders.
文摘广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)为姜科姜黄属植物.通过抑菌实验和电镜技术等方法测定研究了广西莪术油对6种植物病原真菌的抑制作用,结果表明:广西莪术油具有较强的抗真菌活性,对植物病原真菌孢子萌发和菌丝生长均有显著的抑制作用.最低抑制浓度(MIC)的莪术油6.25mL/L对植物病原真菌孢子萌发和菌丝生长均有显著的抑制作用.半抑制率IC50表明广西莪术油对松赤枯病菌的抑制作用最强,最弱的是小麦赤霉病菌.电镜下观察菌丝的横切面表明,处理菌丝的细胞壁消失,原生质解体.研究结果表明广西莪术具有开发为生物农药的潜力.
文摘目的探究广西莪术(Curcuma kwangsiensis S.G.Lee et C.F.Liang)乙酸乙酯部位的抗血栓作用。方法建立角叉菜胶诱导小鼠尾血栓模型、大鼠动静脉旁路血栓模型、FeCl_3诱导大鼠体内血栓模型,观察该部位抗血栓形成的作用,ELISA法检测大鼠血清中ET-1、6-keto-PGF_(1α)、TXB2水平,硝酸还原酶法测定NO水平。再建立大鼠急性血瘀模型,观察该部位对血液流变学的影响。结果广西莪术乙酸乙酯部位可显著减少角叉菜胶诱导小鼠黑尾血栓数量和黑尾长度;大鼠动静脉旁路血栓模型、FeCl_3诱导体内血栓模型的血栓湿重显著降低,血清中NO、6-keto-PGF_(1α)水平增加,ET-1、TXB2水平降低;大鼠急性血瘀模型的全血黏度、血浆黏度显著降低。结论广西莪术乙酸乙酯部位具有显著的抗血栓形成作用,其机制可能与提高NO、6-keto-PGF_(1α)水平,减少ET-1、TXB2水平,降低全血黏度、血浆黏度有关。
