Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often ...Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often posing diagnostic challenges.Dermoscopy and reflectance confocal microscopy(RCM)can aid in their differentiation,yet their specific features under these tools have not been systematically described.This study aims to summarize and analyze the dermoscopic and RCM features of VEN,SK,VP,VV,and NS.Methods:A total of 121 patients with histopathologically confirmed verrucous proliferative skin diseases were enrolled.Dermoscopy and RCM imaging was used to observe and analyze the microscopic features of these conditions.Results:Under dermoscopy,the 5 diseases displayed distinct characteristics:VEN typically showed gyriform structures;SK was characterized by gyriform structures,comedo-like openings,and milia-like cysts;VP and VV featured dotted vessels and frogspawn-like structures;NS presented as brownish-yellow globules.RCM revealed shared features such as hyperkeratosis and acanthosis across all 5 diseases.Specific features included gyriform structures and elongated rete ridges in VEN;pseudocysts and gyriform structures in SK;evenly distributed ring-like structures in VP;vacuolated cells and papillomatous proliferation in VV;and frogspawn-like structures in NS.Conclusion:These 5 verrucous proliferative skin conditions exhibit distinguishable features under both dermoscopy and RCM.The combination of these 2 noninvasive imaging modalities holds significant clinical value for the differential diagnosis of verrucous proliferative skin diseases.展开更多
The understanding of the structure morphology of oil-rich emulsion from enzyme-assisted extraction processing(EAEP)was a critical step to break the oil-rich emulsion structure in order to recover oil.Albeit EAEP metho...The understanding of the structure morphology of oil-rich emulsion from enzyme-assisted extraction processing(EAEP)was a critical step to break the oil-rich emulsion structure in order to recover oil.Albeit EAEP method has been applied as an alternative way to conventional solvent extraction method,the structure morphology of oil-rich emulsion was still unclear.The current study aimed to investigate the structure morphology of oil-rich emulsion from EAEP using 3 D confocal Raman imaging technique.With increasing the enzymatic hydrolysis duration from 1 to 3 h,the stability of oil-rich emulsion was decreased as visualized in the 3 D confocal Raman images that the protein and oil were mixed together.The subsequent Raman spectrum analysis further revealed that the decreased stability of oil-rich emulsion was due to the protein aggregations via SS bonds or protein-lipid interactions.The conformational transfer in protein indicated the formation of a compact structure.展开更多
In this paper,Sulfobacillus thermosulfidooxidans ST was selected for use in bioleaching of pyrite and chalcopyrite.The adsorption experiments revealed that more cells were adsorbed on the surface of pyrite than on the...In this paper,Sulfobacillus thermosulfidooxidans ST was selected for use in bioleaching of pyrite and chalcopyrite.The adsorption experiments revealed that more cells were adsorbed on the surface of pyrite than on the surface of chalcopyrite.The role of extracellular DNA(eDNA)in the bioleaching process was investigated by depletion of eDNA using DNase I.The number of cells attached on the chalcopyrite and pyrite surfaces decreased on a large scale,and the lag phase of cell growth increased,causing the leaching percentages of pyrite and chalcopyrite to decrease by approximately 11.6%and 20.5%,respectively.The formation and distribution of eDNA secreted during bioleaching was assessed by a fluorescent dye-based method and visualized by confocal laser scanning microscopy(CLSM).The content of eDNA increased with bioleaching time.Furthermore,ST showed a stronger capacity to produce eDNA on the surface of pyrite than on the surface of chalcopyrite.These results showed that the removal of eDNA has a more significant effect on the bioleaching of chalcopyrite than on pyrite.展开更多
Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser sc...Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser scanning microscope, and the austenite isothermal and non-isothermal transformation kinetics were studied by dilatometry. The results indicate that banded structure is produced for the reason of composition segregation and the competition between recrystallization and phase transformation. Austenite prefers to nucleate not only at ferrite/ferrite grain boundaries, but also inside the grains of ferrite.Furthermore, the austenitizing process is accomplished mainly via migration of the existing austenite/ferrite interface rather than nucleation of new grains. The incubation process can be divided into two stages which are controlled by carbon and manganese diffusion, respectively. During the incubation process, the nucleation rate of austenite decreases, and austenite growth changes from two-dimensional to one-dimensional. The partitioning coefficient, defined as the ratio of manganese content in the austenite to that in the adjacent ferrite, increases with increasing soaking time.展开更多
RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice....RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice.Immunofluorescence results showed that RanBP1 was mainly localized in cytoplasm at mitosis interphase,and its concentration was lower in nucleus and the lowest in nucleolus.With the formation of the spindle in the early embryonic cells,RanBP1 condensed area took the shape of spindle microtubule,the concentration of RanBP1 was low in the site of chromosome.During the formation of nucleus,RanBP1 concentrated in nucleus and there were few dots of RanBP1 around the nucleolus.These dots were lost after the nucleus full growth.The results showed that RanBP1 had important roles in nucleocytoplasmic transport,spindle formation and nuclear assembly in the early embryonic development of mice.展开更多
Bacillus thuringiensis(Bt)exhibits strong insecticidal activity and is harmless to non-target organisms such as human and animals.Bt becomes the most commonly used environment-friendly insecticidal microorganism.Howev...Bacillus thuringiensis(Bt)exhibits strong insecticidal activity and is harmless to non-target organisms such as human and animals.Bt becomes the most commonly used environment-friendly insecticidal microorganism.However,the insecticidal activities of different Bt strains variy significantly.Therefore,it is particularly important to compare the insecticidal activities of different strains and explore their insecticidal effector mechanisms to expand Bt insecticidal spectrum and enrich transgenic resources.Here,the insecticidal activities of Vip3Aa57 and Vip3Aa62 strains,carrying vegetative insecticidal protein-encoding genes that were inserted into the expression vector pET-21b and transformed into Escherichia coli Rosetta(DE3)strain,expressing 88 ku protein,were compared.Vip3Aa57 protein reportedly displayed body weight inhibition effect on Spodoptera exigua without affecting Heliothis armigera while Vip3Aa62 protein was known to have strong insecticidal activity against S.exigua(LC50=5.124 ng•mg^(-1)).A low H.armigera activity(LC50=870.1 ng•mg^(-1))was observed.The paraffin sectioning results showed that Vip3Aa57 protein affected S.exigua midgut cell morphology.The laser confocal microscopic imaging results showed that Vip3Aa57 bound to receptors in the midgut without damaging the midgut tissue morphology.This study would be conducive for making full use of Bt strains in the soil to compare the insecticidal activities of different Vip insecticidal genes.It could thus provide significant help in revealing the underlying insecticidal mechanisms of Vip3Aa insecticidal genes,developing new insecticidal proteins and delaying pest resistance problems.展开更多
基金supported by the Project of Health Committee of Hunan Province(D202304128868),China.
文摘Objective:Verrucous epidermal nevus(VEN),seborrheic keratosis(SK),verruca plana(VP),verruca vulgaris(VV),and nevus sebaceous(NS)are common verrucous proliferative skin diseases with similar clinical appearances,often posing diagnostic challenges.Dermoscopy and reflectance confocal microscopy(RCM)can aid in their differentiation,yet their specific features under these tools have not been systematically described.This study aims to summarize and analyze the dermoscopic and RCM features of VEN,SK,VP,VV,and NS.Methods:A total of 121 patients with histopathologically confirmed verrucous proliferative skin diseases were enrolled.Dermoscopy and RCM imaging was used to observe and analyze the microscopic features of these conditions.Results:Under dermoscopy,the 5 diseases displayed distinct characteristics:VEN typically showed gyriform structures;SK was characterized by gyriform structures,comedo-like openings,and milia-like cysts;VP and VV featured dotted vessels and frogspawn-like structures;NS presented as brownish-yellow globules.RCM revealed shared features such as hyperkeratosis and acanthosis across all 5 diseases.Specific features included gyriform structures and elongated rete ridges in VEN;pseudocysts and gyriform structures in SK;evenly distributed ring-like structures in VP;vacuolated cells and papillomatous proliferation in VV;and frogspawn-like structures in NS.Conclusion:These 5 verrucous proliferative skin conditions exhibit distinguishable features under both dermoscopy and RCM.The combination of these 2 noninvasive imaging modalities holds significant clinical value for the differential diagnosis of verrucous proliferative skin diseases.
基金the financial support received from National Natural Science Foundation of China(No.31430067 and 31601475)China Postdoctoral Science Foundation funded project(No.2017M610200)Heilongjiang Postdoctoral Foundation(No.LBH-Z17011)
文摘The understanding of the structure morphology of oil-rich emulsion from enzyme-assisted extraction processing(EAEP)was a critical step to break the oil-rich emulsion structure in order to recover oil.Albeit EAEP method has been applied as an alternative way to conventional solvent extraction method,the structure morphology of oil-rich emulsion was still unclear.The current study aimed to investigate the structure morphology of oil-rich emulsion from EAEP using 3 D confocal Raman imaging technique.With increasing the enzymatic hydrolysis duration from 1 to 3 h,the stability of oil-rich emulsion was decreased as visualized in the 3 D confocal Raman images that the protein and oil were mixed together.The subsequent Raman spectrum analysis further revealed that the decreased stability of oil-rich emulsion was due to the protein aggregations via SS bonds or protein-lipid interactions.The conformational transfer in protein indicated the formation of a compact structure.
基金Projects(31470230,51320105006,51604308)supported by the National Natural Science Foundation of ChinaProject(2017RS3003)supported by the Youth Talent Foundation of Hunan Province of China+1 种基金Project(2018JJ2486)supported by the Natural Science Foundation of Hunan Province of ChinaProject(2018WK2012)supported by the Key Research and Development Projects in Hunan Province,China。
文摘In this paper,Sulfobacillus thermosulfidooxidans ST was selected for use in bioleaching of pyrite and chalcopyrite.The adsorption experiments revealed that more cells were adsorbed on the surface of pyrite than on the surface of chalcopyrite.The role of extracellular DNA(eDNA)in the bioleaching process was investigated by depletion of eDNA using DNase I.The number of cells attached on the chalcopyrite and pyrite surfaces decreased on a large scale,and the lag phase of cell growth increased,causing the leaching percentages of pyrite and chalcopyrite to decrease by approximately 11.6%and 20.5%,respectively.The formation and distribution of eDNA secreted during bioleaching was assessed by a fluorescent dye-based method and visualized by confocal laser scanning microscopy(CLSM).The content of eDNA increased with bioleaching time.Furthermore,ST showed a stronger capacity to produce eDNA on the surface of pyrite than on the surface of chalcopyrite.These results showed that the removal of eDNA has a more significant effect on the bioleaching of chalcopyrite than on pyrite.
基金Project(2013AA031601)supported by the National High Technology Research and Development Program of ChinaProject(2012BAF04B01)supported by the National Science and Technology Pillar Program During the 12th Five-year Plan Period of China
文摘Two different kinds of experimental techniques were used to in-situ study the austenite formation during intercritical annealing in C-Mn dual phase steel. The microstructure evolution was observed by confocal laser scanning microscope, and the austenite isothermal and non-isothermal transformation kinetics were studied by dilatometry. The results indicate that banded structure is produced for the reason of composition segregation and the competition between recrystallization and phase transformation. Austenite prefers to nucleate not only at ferrite/ferrite grain boundaries, but also inside the grains of ferrite.Furthermore, the austenitizing process is accomplished mainly via migration of the existing austenite/ferrite interface rather than nucleation of new grains. The incubation process can be divided into two stages which are controlled by carbon and manganese diffusion, respectively. During the incubation process, the nucleation rate of austenite decreases, and austenite growth changes from two-dimensional to one-dimensional. The partitioning coefficient, defined as the ratio of manganese content in the austenite to that in the adjacent ferrite, increases with increasing soaking time.
基金Supported by Heilongjiang Provincial Natural Science Foundation(Face Project)(C2016021)Open Project of Key Laboratory of Feed Science,Northeast Agricultural University,Heilongjiang Province(yy-2012-10)。
文摘RanBP1 is a binding protein of Ran that plays a pivotal role in nucleocytoplasmic transport.In this study,the localization and possible functions of RanBP1 were examined,during the early embryonic development of mice.Immunofluorescence results showed that RanBP1 was mainly localized in cytoplasm at mitosis interphase,and its concentration was lower in nucleus and the lowest in nucleolus.With the formation of the spindle in the early embryonic cells,RanBP1 condensed area took the shape of spindle microtubule,the concentration of RanBP1 was low in the site of chromosome.During the formation of nucleus,RanBP1 concentrated in nucleus and there were few dots of RanBP1 around the nucleolus.These dots were lost after the nucleus full growth.The results showed that RanBP1 had important roles in nucleocytoplasmic transport,spindle formation and nuclear assembly in the early embryonic development of mice.
基金Supported by the National Key R&D Projects(2017YFD0201200)。
文摘Bacillus thuringiensis(Bt)exhibits strong insecticidal activity and is harmless to non-target organisms such as human and animals.Bt becomes the most commonly used environment-friendly insecticidal microorganism.However,the insecticidal activities of different Bt strains variy significantly.Therefore,it is particularly important to compare the insecticidal activities of different strains and explore their insecticidal effector mechanisms to expand Bt insecticidal spectrum and enrich transgenic resources.Here,the insecticidal activities of Vip3Aa57 and Vip3Aa62 strains,carrying vegetative insecticidal protein-encoding genes that were inserted into the expression vector pET-21b and transformed into Escherichia coli Rosetta(DE3)strain,expressing 88 ku protein,were compared.Vip3Aa57 protein reportedly displayed body weight inhibition effect on Spodoptera exigua without affecting Heliothis armigera while Vip3Aa62 protein was known to have strong insecticidal activity against S.exigua(LC50=5.124 ng•mg^(-1)).A low H.armigera activity(LC50=870.1 ng•mg^(-1))was observed.The paraffin sectioning results showed that Vip3Aa57 protein affected S.exigua midgut cell morphology.The laser confocal microscopic imaging results showed that Vip3Aa57 bound to receptors in the midgut without damaging the midgut tissue morphology.This study would be conducive for making full use of Bt strains in the soil to compare the insecticidal activities of different Vip insecticidal genes.It could thus provide significant help in revealing the underlying insecticidal mechanisms of Vip3Aa insecticidal genes,developing new insecticidal proteins and delaying pest resistance problems.
