The control and prevention of pasteurellosis in rabbits which makes the hosts unsuitable for experimental use raised the needs to improve and simplify the procedures of Enzyme-Linked Immunosorbent Assay (ELISA) for de...The control and prevention of pasteurellosis in rabbits which makes the hosts unsuitable for experimental use raised the needs to improve and simplify the procedures of Enzyme-Linked Immunosorbent Assay (ELISA) for detect of antibody against P.multocida. A comparison on the sensitivity and specificity of bacterial culture of antemortem and postmortem samples, complement fixation test and enzyme-linked immunosorbent assay of 11 apparently healthy adult rabbits was conducted. The incidence rates showed 45.45%,54.54% and 72.73% respectively. The sensitivity for the three methods were 0.63, 0.67,and 1.00,and specificity for them were 1.00, 0.67 and 1.00 respectively. Somatic serotypes of isolates of P.multocida from rabbits of three groups (rabbits of group 2 were with clinic signs, those of groups 1 and 3 were apparently healthy) revealed no remarkable differences,and the predominant types were type 3 and type 3, 4. This was somewhat different from the reports derived from other states. As the antigen of different serotype used in ELISA may have different sensitivity and specificity, which is affected also by different preparation method, a type non-specific antigen should be selected to meet such request. The trial of accomplishment of ELISA without positive and negative controls was presented for discussion.展开更多
In order to avoid severe performance degradation led by the inter-cell interference (ICI) in orthogonal frequency division multiple access (OFDMA) systems with a frequency reused factor (FRF) of 1,distributed schedule...In order to avoid severe performance degradation led by the inter-cell interference (ICI) in orthogonal frequency division multiple access (OFDMA) systems with a frequency reused factor (FRF) of 1,distributed schedule algorithm (DS-OCS) and distributed proportional fairness schedule algorithm (DPFS-OCS) based on orthogonal complement space (OCS) were proposed. The first right and left singular vectors of the channel that the user experienced were selected as the transmitting and receiving beamforming vectors. An interference space was spanned by the left singular vectors of the entire interference users in the same channel. The most suitable user lay in the OCS of the interference space was scheduled to avoid suffering interference from neighboring cells based on the criterion of system capacity maximizing and proportional fairness. The simulation results show that the average system capacity can be improved by 2%-4% compared with the DS-OCS algorithm with the Max C/I algorithm,by 6%-10% compared with the DPFS-OCS algorithm with the PF algorithm.展开更多
文摘The control and prevention of pasteurellosis in rabbits which makes the hosts unsuitable for experimental use raised the needs to improve and simplify the procedures of Enzyme-Linked Immunosorbent Assay (ELISA) for detect of antibody against P.multocida. A comparison on the sensitivity and specificity of bacterial culture of antemortem and postmortem samples, complement fixation test and enzyme-linked immunosorbent assay of 11 apparently healthy adult rabbits was conducted. The incidence rates showed 45.45%,54.54% and 72.73% respectively. The sensitivity for the three methods were 0.63, 0.67,and 1.00,and specificity for them were 1.00, 0.67 and 1.00 respectively. Somatic serotypes of isolates of P.multocida from rabbits of three groups (rabbits of group 2 were with clinic signs, those of groups 1 and 3 were apparently healthy) revealed no remarkable differences,and the predominant types were type 3 and type 3, 4. This was somewhat different from the reports derived from other states. As the antigen of different serotype used in ELISA may have different sensitivity and specificity, which is affected also by different preparation method, a type non-specific antigen should be selected to meet such request. The trial of accomplishment of ELISA without positive and negative controls was presented for discussion.
基金Projects(2009ZX03003-003, 2009ZX03003-004) supported by the Major National Science & Technology ProgramProject(B08038) supported by the "111" Project+1 种基金Project(HX0109012417) supported by Huawei Technologies Co., Ltd, ChinaProject(IRT0852) supported by Program for Changjiang Scholars and Innovative Research Team in Chinese University
文摘In order to avoid severe performance degradation led by the inter-cell interference (ICI) in orthogonal frequency division multiple access (OFDMA) systems with a frequency reused factor (FRF) of 1,distributed schedule algorithm (DS-OCS) and distributed proportional fairness schedule algorithm (DPFS-OCS) based on orthogonal complement space (OCS) were proposed. The first right and left singular vectors of the channel that the user experienced were selected as the transmitting and receiving beamforming vectors. An interference space was spanned by the left singular vectors of the entire interference users in the same channel. The most suitable user lay in the OCS of the interference space was scheduled to avoid suffering interference from neighboring cells based on the criterion of system capacity maximizing and proportional fairness. The simulation results show that the average system capacity can be improved by 2%-4% compared with the DS-OCS algorithm with the Max C/I algorithm,by 6%-10% compared with the DPFS-OCS algorithm with the PF algorithm.
