The assembly of a protein complex is very important for its biological function,which can be investigated by determining the order of assembly/disassembly of its protein subunits.Although static structures of many pro...The assembly of a protein complex is very important for its biological function,which can be investigated by determining the order of assembly/disassembly of its protein subunits.Although static structures of many protein com-plexes are available in the protein data bank,their assembly/disassembly orders of subunits are largely unknown.In addition to experimental techniques for studying subcomplexes in the assembly/disassembly of a protein complex,computational methods can be used to predict the assembly/disassembly order.Since sampling is a nontrivial issue in simulating the assembly/disassembly process,coarse-grained simulations are more efficient than atomic simulations are.In this work,we developed computational protocols for predicting the assembly/disassembly orders of protein complexes via coarse-grained simulations.The protocols were illustrated via two protein complexes,and the predicted assembly/disassembly orders were consistent with the available experimental data.展开更多
Aseries of [(Fe_(0.6)Co_(0.2)Ni_(0.2))_(0.75-0.03x)B_(0.2)Si_(0.05+0.03x)]_(96)Nb_(4) amorphous alloy composite coatings were prepared by adjusting the silicon content(x=0,1,2,3,4,5,and 6)and their microstructures and...Aseries of [(Fe_(0.6)Co_(0.2)Ni_(0.2))_(0.75-0.03x)B_(0.2)Si_(0.05+0.03x)]_(96)Nb_(4) amorphous alloy composite coatings were prepared by adjusting the silicon content(x=0,1,2,3,4,5,and 6)and their microstructures and tribological properties were investigated by laser cladding technique.Additionally,the effect of Si on the glass forming ability(GFA)of the layers was understood.Results show that an appropriate Si content can refine the microstructure of the FeCoNiBSiNb laser cladding layers and improve the mechanical and tribological properties.The hardness of the coating layer increases monotonically with the Si content.At the Si content of 4.8at%(x=0),the coating layer exhibits a relatively low hardness(734.2HV 0.1).Conversely,at the silicon content of 13.44at%(x=3),the coating layer exhibits the highest hardness(1106HV 0.1).The non-crystalline content and tensile strength exhibit an initial increase,followed by a subsequent decrease.At x=2,the coating exhibits its maximum fracture strength(2880 MPa).However,when x>2,the fracture strength of the coating decreases with an increase in x.Conversely,with an increase in Si content,the wear volume loss initially decreases and then increases.At a Si content of 10.56at%(x=2),the coating exhibits the highest non-crystalline content(42%),the highest tensile strength(2880 MPa),and the most favorable dry friction performance.展开更多
To evaluate the geotechnical properties of coarse-grained soil affected by cyclic freeze-thaw,the electrical resistivity and mechanical tests are conducted.The soil specimens are prepared under different water content...To evaluate the geotechnical properties of coarse-grained soil affected by cyclic freeze-thaw,the electrical resistivity and mechanical tests are conducted.The soil specimens are prepared under different water contents,dry densities and exposed to 0?20 freeze-thaw cycles.As a result,the stress?strain behavior of the specimen(w=14.0%andρd=1.90 g/cm^3)changes from strain-hardening into strain-softening due to the freeze-thaw effect.The electrical resistivity of test specimen increases with the freeze-thaw cycles change,but the mechanical parameters(the unconfined compressive strength qu and the deformation modulus E)and brittleness index decrease considerably at the same conditions.All of them tend to be stable after 7?9 cycles.Moreover,both the dry density and the water content have reciprocal effects on the freeze-thaw actions.The failure and pore characteristics of specimens affected by freeze-thaw cycles are discussed by using the image analysis method.Then,an exponential function equation is developed to assess the electrical resistivity of specimens affected by the cyclic freeze-thaw.Linear relations between the mechanical parameters and the electrical resistivity of specimens are established to evaluate the geotechnical properties of the soil exposed to freeze-thaw actions through the corresponding electrical resistivity.展开更多
In order to investigate the influence of intermediate principal stress on the stress-strain and strength behaviour of a coarse-grained soil, a series of true triaxial tests were performed. The tests were conducted in ...In order to investigate the influence of intermediate principal stress on the stress-strain and strength behaviour of a coarse-grained soil, a series of true triaxial tests were performed. The tests were conducted in a recently developed true triaxial apparatus with constant minor principal stress σ3 and constant value of intermediate principal stress ratio b=（σ2-σ3）/（σ1-σ3） （al is the vertical stress, and % is the horizontal stress）. It is found that the intermediate principal strain, ε2, increases from negative to positive value with the increase of parameter b from zero to unity under a constant minor principal stress. The minor principal strain, ε3, is always negative. This implies that the specimen exhibits an evident anisotropy. The relationship between b and friction angle obtained from the tests is different from that predicted by LADE-DUNCAN and MATSUOKA-NAKAI criteria. Based on the test results, an empirical equation of g（b） that is the shape function of the failure surface on re-plane was presented. The proposed equation is verified to be reasonable by comparing the predicted results using the equation with true triaxial test results of soils, such as coarse-grained soils in this study, sands and gravels in other studies.展开更多
Gradation equation is one way to describe the gradation of coarse-grained soil conveniently,exactly and quantitatively.With the gradation equation,the influence of gradation on the mechanical behaviors of coarse-grain...Gradation equation is one way to describe the gradation of coarse-grained soil conveniently,exactly and quantitatively.With the gradation equation,the influence of gradation on the mechanical behaviors of coarse-grained soil can be expressed quantitatively.A new gradation equation with a parameter is proposed.The basic properties and applicability of the new equation are studied.The results show that the proposed equation has the applicability to express coarse-grained soil gradation(CSG),and the range of the parameter β is found to be 0<β<1.The value ofbdetermines the gradation curve shape.If β>0.5,the gradation curve is sigmoidal,otherwise the gradation curve is hyperbolic.For well graded gradations,the parameter has the value of 0.13<β<1.Several CSGs used in domestic and foreign earth-rockfill dams are probed,and the value of the parameterbfalls in the range of 0.18 to 0.97.The investigation of the range of β is of value to guide the design for CSG of earth-rockfill dam.展开更多
By using large scale triaxial shearing apparatus,consolidated-drained shear tests were conducted on coarse-grained soil with different gradations.In order to describe their deformation rules,three main characteristics...By using large scale triaxial shearing apparatus,consolidated-drained shear tests were conducted on coarse-grained soil with different gradations.In order to describe their deformation rules,three main characteristics of tangent Poisson ratio curves were summarized and the reason was revealed by dividing the movement of soil particles into two kinds: the movement of fine particles and the movement of coarse particles.Then,a volumetric strain expression and a tangent Poisson ratio expression were put forward,and two defects of widely used Duncan-Chang model were fixed.Results calculated from them agree well with test results.There are three parameters,namely L,G and F,in this new model.Parameter L reflects the dilatancy of a specimen and L=4 can be used as a criterion to estimate whether a certain kind of soil has dilatancy quality or not.Parameters G and F relate to the initial slope of tangent Poisson ratio curves,and G=F=0 indicates a special situation which happens in dense granular material of the same diameter.Influences of various gradations on volume deformation are mainly reflected in parameter L which is smaller when there are more gravels in specimens.展开更多
Limited research has been conducted on the influences of fiber content on close-in blasting characteristics for ultrahigh-performance fiber-reinforced concrete(UHPFRC)beams.This paper aims to address this knowledge ga...Limited research has been conducted on the influences of fiber content on close-in blasting characteristics for ultrahigh-performance fiber-reinforced concrete(UHPFRC)beams.This paper aims to address this knowledge gap through experimental and mesoscale numerical methods.Experiments were conducted on ten UHPFRC beams built with varying steel fiber volumetric fractions subjected to close-in explosive conditions.Additionally,this study considered other parameters,such as the longitudinal reinforcement type and ratio.In the case of UHPFRC beams featuring normal-strength longitudinal reinforcement of diametersΦ12,Φ16,andΦ20,a reduction in maximum displacement by magnitudes of19.6%,19.5%,and 17.4%was observed,respectively,as the volumetric fractions of fiber increased from1.0%to 2.5%.In addition,increasing the longitudinal reinforcement ratio and using high-strength steel longitudinal reinforcement both significantly reduced the deformation characteristics and increase the blasting resistances of UHPFRC beams.However,the effects on the local crushing and spalling damage were not significant.A mesoscale finite element model,which considers the impacts of fiber parameters on UHPFRC beam behaviors,was also established and well correlated with the test findings.Nevertheless,parametric analyses were further conducted to examine the impacts of the steel fiber content and length and the hybrid effects of various types of microfibers and steel fibers on the blasting performance of UHPFRC beams.展开更多
In order to study the effects of biochar-based organic fertilizer on the accumulation of the total flavonoid contents in dandelion after sowing,the experiment applied four levels of treatments of carbon-based organic ...In order to study the effects of biochar-based organic fertilizer on the accumulation of the total flavonoid contents in dandelion after sowing,the experiment applied four levels of treatments of carbon-based organic fertilizer(0.15,0.30,0.45,and 0.60 kg•m^(-2)),and the base fertilizer that only applied conventional chemical fertilizer was used as the control.The total flavonoid contents and physiological indices of dandelion in different growth periods were determined.The results showed that the accumulation of the total flavonoid contents in leaves and roots of dandelion improved significantly by applying biochar-based organic fertilizer(0.15,0.30,0.45,and 0.60 kg•m^(-2)),with the highest total flavonoid occurred in the 0.45 kg•m^(-2) biochar-based organic fertilizer treatment.Along with dandelion growing,the total flavonoid contents in leaves were first elevated,and then decreased,with the maximum reached at 95 days after sowing(anthesis).While the total flavonoid contents in roots were elevated constantly.The total flavonoid contents accumulation in dandelion were positively correlated with net photosynthetic rate,stomatal conductance,transpiration rate,soluble sugar content,soluble protein,nitrate nitrogen,and available potassium,while negatively correlated with intercellular CO_(2) concentration.展开更多
In challenging situations,such as low illumination,rain,and background clutter,the stability of the thermal infrared(TIR)spectrum can help red,green,blue(RGB)visible spectrum to improve tracking performance.However,th...In challenging situations,such as low illumination,rain,and background clutter,the stability of the thermal infrared(TIR)spectrum can help red,green,blue(RGB)visible spectrum to improve tracking performance.However,the high-level image information and the modality-specific features have not been sufficiently studied.The proposed correlation filter uses the fused saliency content map to improve filter training and extracts different features of modalities.The fused content map is intro-duced into the spatial regularization term of correlation filter to highlight the training samples in the content region.Furthermore,the fused content map can avoid the incompleteness of the con-tent region caused by challenging situations.Additionally,differ-ent features are extracted according to the modality characteris-tics and are fused by the designed response-level fusion stra-tegy.The alternating direction method of multipliers(ADMM)algorithm is used to solve the tracker training efficiently.Experi-ments on the large-scale benchmark datasets show the effec-tiveness of the proposed tracker compared to the state-of-the-art traditional trackers and the deep learning based trackers.展开更多
通过比较不同基质中药用石斛生长量和有效成份质量分数,探究经过无害化处理的松材线虫病疫木在铁皮石斛(Dendrobium officinale Kimura et Migo)人工栽培中作为配方基质的使用效果。结果表明:在铁皮石斛人工种植中使用经过无害化处理后...通过比较不同基质中药用石斛生长量和有效成份质量分数,探究经过无害化处理的松材线虫病疫木在铁皮石斛(Dendrobium officinale Kimura et Migo)人工栽培中作为配方基质的使用效果。结果表明:在铁皮石斛人工种植中使用经过无害化处理后的松材线虫病疫木作为栽培基质切实可行。不同栽培方式适用的松材线虫病疫木替代基质的比例不同。以盆栽方式种植铁皮石斛时,1号基质(V(粒径9~12 mm疫木颗粒)∶V(新鲜树皮)∶V(饼肥)=1∶1∶1)充分发酵后可以在实际生产中替代常规栽培基质使用;以盘栽方式种植铁皮石斛时,3号基质(V(疫木锯末)∶V(新鲜树皮)∶V(饼肥)=1∶1∶1)充分发酵后可以在实际生产中替代常规栽培基质使用。展开更多
基金This work was supported by the National Key Research and Development Program of China(2021YFA1301504)the Chinese Academy of Sciences Strategic Priority Research Program(XDB37040202)the National Natural Science Foundation of China(91953101).
文摘The assembly of a protein complex is very important for its biological function,which can be investigated by determining the order of assembly/disassembly of its protein subunits.Although static structures of many protein com-plexes are available in the protein data bank,their assembly/disassembly orders of subunits are largely unknown.In addition to experimental techniques for studying subcomplexes in the assembly/disassembly of a protein complex,computational methods can be used to predict the assembly/disassembly order.Since sampling is a nontrivial issue in simulating the assembly/disassembly process,coarse-grained simulations are more efficient than atomic simulations are.In this work,we developed computational protocols for predicting the assembly/disassembly orders of protein complexes via coarse-grained simulations.The protocols were illustrated via two protein complexes,and the predicted assembly/disassembly orders were consistent with the available experimental data.
文摘Aseries of [(Fe_(0.6)Co_(0.2)Ni_(0.2))_(0.75-0.03x)B_(0.2)Si_(0.05+0.03x)]_(96)Nb_(4) amorphous alloy composite coatings were prepared by adjusting the silicon content(x=0,1,2,3,4,5,and 6)and their microstructures and tribological properties were investigated by laser cladding technique.Additionally,the effect of Si on the glass forming ability(GFA)of the layers was understood.Results show that an appropriate Si content can refine the microstructure of the FeCoNiBSiNb laser cladding layers and improve the mechanical and tribological properties.The hardness of the coating layer increases monotonically with the Si content.At the Si content of 4.8at%(x=0),the coating layer exhibits a relatively low hardness(734.2HV 0.1).Conversely,at the silicon content of 13.44at%(x=3),the coating layer exhibits the highest hardness(1106HV 0.1).The non-crystalline content and tensile strength exhibit an initial increase,followed by a subsequent decrease.At x=2,the coating exhibits its maximum fracture strength(2880 MPa).However,when x>2,the fracture strength of the coating decreases with an increase in x.Conversely,with an increase in Si content,the wear volume loss initially decreases and then increases.At a Si content of 10.56at%(x=2),the coating exhibits the highest non-crystalline content(42%),the highest tensile strength(2880 MPa),and the most favorable dry friction performance.
基金Project(2016ZGHJ/XZHTL-YQSC-26)supported by the Key Scientific Research Project of China Gold GroupProject(SQ2019QZKK2806)supported by the Second Tibetan Plateau Scientific Expedition and Research(STEP)Program,China+1 种基金Project(300102268716)supported by the Fundamental Research Funds for the Central Universities,ChinaProject(LHKA-G201701)supported by the Science and Technology Project of Yalong River Hydropower Development Company,China。
文摘To evaluate the geotechnical properties of coarse-grained soil affected by cyclic freeze-thaw,the electrical resistivity and mechanical tests are conducted.The soil specimens are prepared under different water contents,dry densities and exposed to 0?20 freeze-thaw cycles.As a result,the stress?strain behavior of the specimen(w=14.0%andρd=1.90 g/cm^3)changes from strain-hardening into strain-softening due to the freeze-thaw effect.The electrical resistivity of test specimen increases with the freeze-thaw cycles change,but the mechanical parameters(the unconfined compressive strength qu and the deformation modulus E)and brittleness index decrease considerably at the same conditions.All of them tend to be stable after 7?9 cycles.Moreover,both the dry density and the water content have reciprocal effects on the freeze-thaw actions.The failure and pore characteristics of specimens affected by freeze-thaw cycles are discussed by using the image analysis method.Then,an exponential function equation is developed to assess the electrical resistivity of specimens affected by the cyclic freeze-thaw.Linear relations between the mechanical parameters and the electrical resistivity of specimens are established to evaluate the geotechnical properties of the soil exposed to freeze-thaw actions through the corresponding electrical resistivity.
基金Project(50639050) supported by the National Natural Science Foundation of China and Er-Tan Hydraulicpower Limited CompanyProject(50579014) supported by the National Natural Science Foundation of China+3 种基金Project(09KJD560003) supported by the Natural Science Foundation of Jiangsu Higher Education Institutions of ChinaProject(BK2007582) supported by Jiangsu Provincial Natural Science Foundation of ChinaProject(20070294002) supported by the Specialized Research Fund for the Doctoral Program of Higher Education of ChinaProject(GH200904) supported by Key Laboratory of Ministry of Education for Geomechanics and Embankment Engineering,Hohai University,China
文摘In order to investigate the influence of intermediate principal stress on the stress-strain and strength behaviour of a coarse-grained soil, a series of true triaxial tests were performed. The tests were conducted in a recently developed true triaxial apparatus with constant minor principal stress σ3 and constant value of intermediate principal stress ratio b=（σ2-σ3）/（σ1-σ3） （al is the vertical stress, and % is the horizontal stress）. It is found that the intermediate principal strain, ε2, increases from negative to positive value with the increase of parameter b from zero to unity under a constant minor principal stress. The minor principal strain, ε3, is always negative. This implies that the specimen exhibits an evident anisotropy. The relationship between b and friction angle obtained from the tests is different from that predicted by LADE-DUNCAN and MATSUOKA-NAKAI criteria. Based on the test results, an empirical equation of g（b） that is the shape function of the failure surface on re-plane was presented. The proposed equation is verified to be reasonable by comparing the predicted results using the equation with true triaxial test results of soils, such as coarse-grained soils in this study, sands and gravels in other studies.
基金Project(2018YFC1508505)supported by the National Key Research and Development Program of ChinaProject(U1865104)supported by Yalong River Joint Fund of Natural Science Foundation of China-Yalong River Basin Hydropower Development Co.,Ltd.,China+1 种基金Project(51479052)supported by National Natural Science of ChinaProject(2019T120443)supported by China Postdoctoral Science Foundation。
文摘Gradation equation is one way to describe the gradation of coarse-grained soil conveniently,exactly and quantitatively.With the gradation equation,the influence of gradation on the mechanical behaviors of coarse-grained soil can be expressed quantitatively.A new gradation equation with a parameter is proposed.The basic properties and applicability of the new equation are studied.The results show that the proposed equation has the applicability to express coarse-grained soil gradation(CSG),and the range of the parameter β is found to be 0<β<1.The value ofbdetermines the gradation curve shape.If β>0.5,the gradation curve is sigmoidal,otherwise the gradation curve is hyperbolic.For well graded gradations,the parameter has the value of 0.13<β<1.Several CSGs used in domestic and foreign earth-rockfill dams are probed,and the value of the parameterbfalls in the range of 0.18 to 0.97.The investigation of the range of β is of value to guide the design for CSG of earth-rockfill dam.
基金Project(50908233)supported by the National Natural Science Foundation of ChinaProject(2008G031-Q)supported by National Engineering Laboratory for High Speed Railway Construction,China
文摘By using large scale triaxial shearing apparatus,consolidated-drained shear tests were conducted on coarse-grained soil with different gradations.In order to describe their deformation rules,three main characteristics of tangent Poisson ratio curves were summarized and the reason was revealed by dividing the movement of soil particles into two kinds: the movement of fine particles and the movement of coarse particles.Then,a volumetric strain expression and a tangent Poisson ratio expression were put forward,and two defects of widely used Duncan-Chang model were fixed.Results calculated from them agree well with test results.There are three parameters,namely L,G and F,in this new model.Parameter L reflects the dilatancy of a specimen and L=4 can be used as a criterion to estimate whether a certain kind of soil has dilatancy quality or not.Parameters G and F relate to the initial slope of tangent Poisson ratio curves,and G=F=0 indicates a special situation which happens in dense granular material of the same diameter.Influences of various gradations on volume deformation are mainly reflected in parameter L which is smaller when there are more gravels in specimens.
基金supported by the National Natural Science Foundation of China(Grant No.12102050)the Open Fund of State Key Laboratory of Explosion Science and Technology(Grant No.SKLEST-ZZ-21-18)。
文摘Limited research has been conducted on the influences of fiber content on close-in blasting characteristics for ultrahigh-performance fiber-reinforced concrete(UHPFRC)beams.This paper aims to address this knowledge gap through experimental and mesoscale numerical methods.Experiments were conducted on ten UHPFRC beams built with varying steel fiber volumetric fractions subjected to close-in explosive conditions.Additionally,this study considered other parameters,such as the longitudinal reinforcement type and ratio.In the case of UHPFRC beams featuring normal-strength longitudinal reinforcement of diametersΦ12,Φ16,andΦ20,a reduction in maximum displacement by magnitudes of19.6%,19.5%,and 17.4%was observed,respectively,as the volumetric fractions of fiber increased from1.0%to 2.5%.In addition,increasing the longitudinal reinforcement ratio and using high-strength steel longitudinal reinforcement both significantly reduced the deformation characteristics and increase the blasting resistances of UHPFRC beams.However,the effects on the local crushing and spalling damage were not significant.A mesoscale finite element model,which considers the impacts of fiber parameters on UHPFRC beam behaviors,was also established and well correlated with the test findings.Nevertheless,parametric analyses were further conducted to examine the impacts of the steel fiber content and length and the hybrid effects of various types of microfibers and steel fibers on the blasting performance of UHPFRC beams.
基金Supported by the National Key Research and Development Project(2021YFD16009012021YFD1600902)。
文摘In order to study the effects of biochar-based organic fertilizer on the accumulation of the total flavonoid contents in dandelion after sowing,the experiment applied four levels of treatments of carbon-based organic fertilizer(0.15,0.30,0.45,and 0.60 kg•m^(-2)),and the base fertilizer that only applied conventional chemical fertilizer was used as the control.The total flavonoid contents and physiological indices of dandelion in different growth periods were determined.The results showed that the accumulation of the total flavonoid contents in leaves and roots of dandelion improved significantly by applying biochar-based organic fertilizer(0.15,0.30,0.45,and 0.60 kg•m^(-2)),with the highest total flavonoid occurred in the 0.45 kg•m^(-2) biochar-based organic fertilizer treatment.Along with dandelion growing,the total flavonoid contents in leaves were first elevated,and then decreased,with the maximum reached at 95 days after sowing(anthesis).While the total flavonoid contents in roots were elevated constantly.The total flavonoid contents accumulation in dandelion were positively correlated with net photosynthetic rate,stomatal conductance,transpiration rate,soluble sugar content,soluble protein,nitrate nitrogen,and available potassium,while negatively correlated with intercellular CO_(2) concentration.
基金supported by the National Natural Science Foundation of China(62073036,62076031)Beijing Natural Science Foundation(4242049).
文摘In challenging situations,such as low illumination,rain,and background clutter,the stability of the thermal infrared(TIR)spectrum can help red,green,blue(RGB)visible spectrum to improve tracking performance.However,the high-level image information and the modality-specific features have not been sufficiently studied.The proposed correlation filter uses the fused saliency content map to improve filter training and extracts different features of modalities.The fused content map is intro-duced into the spatial regularization term of correlation filter to highlight the training samples in the content region.Furthermore,the fused content map can avoid the incompleteness of the con-tent region caused by challenging situations.Additionally,differ-ent features are extracted according to the modality characteris-tics and are fused by the designed response-level fusion stra-tegy.The alternating direction method of multipliers(ADMM)algorithm is used to solve the tracker training efficiently.Experi-ments on the large-scale benchmark datasets show the effec-tiveness of the proposed tracker compared to the state-of-the-art traditional trackers and the deep learning based trackers.
