Due to the complex high-temperature characteristics of hydrocarbon fuel,the research on the long-term working process of parallel channel structure under variable working conditions,especially under high heat-mass rat...Due to the complex high-temperature characteristics of hydrocarbon fuel,the research on the long-term working process of parallel channel structure under variable working conditions,especially under high heat-mass ratio,has not been systematically carried out.In this paper,the heat transfer and flow characteristics of related high temperature fuels are studied by using typical engine parallel channel structure.Through numeri⁃cal simulation and systematic experimental verification,the flow and heat transfer characteristics of parallel chan⁃nels under typical working conditions are obtained,and the effectiveness of high-precision calculation method is preliminarily established.It is known that the stable time required for hot start of regenerative cooling engine is about 50 s,and the flow resistance of parallel channel structure first increases and then decreases with the in⁃crease of equivalence ratio(The following equivalence ratio is expressed byΦ),and there is a flow resistance peak in the range ofΦ=0.5~0.8.This is mainly caused by the coupling effect of high temperature physical proper⁃ties,flow rate and pressure of fuel in parallel channels.At the same time,the cooling and heat transfer character⁃istics of parallel channels under some conditions of high heat-mass ratio are obtained,and the main factors affect⁃ing the heat transfer of parallel channels such as improving surface roughness and strengthening heat transfer are mastered.In the experiment,whenΦis less than 0.9,the phenomenon of local heat transfer enhancement and deterioration can be obviously observed,and the temperature rise of local structures exceeds 200℃,which is the risk of structural damage.Therefore,the reliability of long-term parallel channel structure under the condition of high heat-mass ratio should be fully considered in structural design.展开更多
In this study,a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin.The probe utilized methylene blue(emission sp...In this study,a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin.The probe utilized methylene blue(emission spectrum range:640-740 nm)and coumarin fluorophore(emission spectrum range:440-600 nm)as signal output units,thereby achieving effective spectral separation and highly selective detection of HClO.Under physiological pH conditions,HClO triggers an oxidation-cleavage reaction,releasing methylene blue and coumarin,which emit distinct red and green fluorescence,respectively.This dual-emission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L^(-1)(green channel)and 31.55 nmol·L^(-1)(red channel).Furthermore,in cell imaging experiments,this probe demonstrated excellent cell membrane permeability and low cytotoxicity,successfully enabling the monitoring of both endogenous and exogenous HClO in living cells.By incorporating a twochannel self-calibration system,the probe effectively mitigated signal variations caused by instrumental or environmental interference,substantially improving detection sensitivity and reliability.展开更多
The work takes a new liquid-cooling plate in a power battery with pin fins inside the channel as the object.A mathematical model is established via the central composite design of the response surface to study the rel...The work takes a new liquid-cooling plate in a power battery with pin fins inside the channel as the object.A mathematical model is established via the central composite design of the response surface to study the relationships among the length,width,height,and spacing of pin fins;the maximum temperature and temperature difference of the battery module;and the pressure drop of the liquid-cooling plate.Model accuracy is verified via variance analysis.The new liquid-cooling plate enables the power battery to work within an optimal temperature range.Appropriately increasing the length,width,and height and reducing the spacing of pin fins could reduce the temperature of the power battery module and improve the temperature uniformity.However,the pressure drop of the liquid-cooling plate increases.The structural parameters of the pin fins are optimized to minimize the maximum temperature and the temperature difference of the battery module as well as the pressure drop of the liquid-cooling plate.The errors between the values predicted and actual by the simulation test are 0.58%,4%,and 0.48%,respectively,which further verifies the model accuracy.The results reveal the influence of the structural parameters of the pin fins inside the liquid-cooling plate on its heat dissipation performance and pressure drop characteristics.A theoretical basis is provided for the design of liquid-cooling plates in power batteries and the optimization of structural parameters.展开更多
For segmented detectors,surface flatness is critical as it directly influences both energy resolution and image clarity.Additionally,the limited adjustment range of the segmented detectors necessitates precise benchma...For segmented detectors,surface flatness is critical as it directly influences both energy resolution and image clarity.Additionally,the limited adjustment range of the segmented detectors necessitates precise benchmark construction.This paper proposes an architecture for detecting detector flatness based on channel spectral dispersion.By measuring the dispersion fringes for coplanar adjustment,the final adjustment residual is improved to better than 300 nm.This result validates the feasibility of the proposed technology and provides significant technical support for the development of next-generation large-aperture sky survey equipment.展开更多
Potassium-calcium activates channel subfamily N member 3(KCNN3/SK3/KCa2.3)is involved in regulating cellular calcium signaling,muscle contraction and neurotransmitter release.Dysregulation of the KCNN3 channel is asso...Potassium-calcium activates channel subfamily N member 3(KCNN3/SK3/KCa2.3)is involved in regulating cellular calcium signaling,muscle contraction and neurotransmitter release.Dysregulation of the KCNN3 channel is associated with the development of various tumors.We use bioinformatics analysis to identify whether KCNN3 regulates the occurrence and development of stomach adenocarcinoma(STAD)as a prognostic target.By analyzing the Human Protein Atlas(HPA)database and The Cancer Genome Atlas(TCGA)database,we found that the protein and mRNA levels of KCNN3 were dramatically reduced in STAD,and TCGA database showed that KCNN3 significantly correlated with the prognosis and clinical features of STAD.In addition,we found that high expression of KCNN3 in STAD reduced the IC 50 of several drugs in STAD cells,suggesting that high expression of KCNN3 correlated with the drug sensitivity of STAD.To investigate the underlying biological mechanism,we identified a potential KCNN3 interaction factor,tumor necrosis factor receptor superfamily member 7(CD27/TNFRSF7),which is expressed at low levels in STAD.RT-qPCR and Western blotting confirmed that KCNN3 and CD27 positively correlated with each other at protein and mRNA levels,and co-immunoprecipitation and immunofluorescence experiments confirmed that the two proteins interact and colocalize in the cytoplasm.Moreover,we confirmed the inhibitory effect of KCNN3 on the proliferation,migration and invasion of human STAD cells in vitro and in vivo through subcutaneous tumorigenesis and cellular experiments.Furthermore,GO/KEGG enrichment analysis showed that KCNN3 was enriched in signaling pathways regulating the immune response and calcium or metal ion transport.Lastly,we verified through cell co-culture,RT-qPCR and CCK8 assays that high expression of KCNN3 can promote the increase of T cell activating factor and the killing effect of T cells on STAD cells.Therefore,our results suggest that KCNN3 is a potential inhibitory factor affecting the occurrence and progression of STAD.展开更多
The Underwater Communication Link(UCL)is a crucial component of Underwater Wireless Optical Communication(UWOC)systems,requiring optimised design to mitigate the high power attenuation inherent in seawater.To ensure t...The Underwater Communication Link(UCL)is a crucial component of Underwater Wireless Optical Communication(UWOC)systems,requiring optimised design to mitigate the high power attenuation inherent in seawater.To ensure the reliability of an optimal UCL design,it is essential to account for the three primary scattering regimes:forward scattering(FSC),backward scattering(BSC),and isotropic scattering(ISC)in seawater channels.This study introduces a new photon-tracking model based on a discrete equation,facilitating Monte Carlo Simulation(MCS)to evaluate how different scattering regimes influence received photon distribution.Three distinct Scattering Regime Contribution Weight(SRCW)probability sets were employed,each representing different UCL operational configurations dominated by specific scattering regimes.The proposed modeling approach enables a comprehensive assessment of the temporal characteristics of received optical pulses,channel loss,and time spread-ultimately defining the optimal UCL design parameters.The key findings of this study include:(1)Enhancing the FSC regime dominance leads to a quasi-light waveguide effect over link spans and small Fields of View(FOV)<25°,significantly improving channel performance in Harbor seawater compared to Coastal seawater.(2)A well-designed UCL with a small FOV(<25°)can minimise channel loss and time spread,ensuring high capacity and efficient performance in both Coastal and Harbor seawaters.(3)When BSC and ISC contributions exceed FSC dominance,the received optical pulse undergoes significant temporal broadening,particularly for larger FOV angles(>25°)and extended link spans.(4)The developed novel MCS-based discrete equation provides a simple yet robust model for simulating photon propagation in both homogeneous and inhomogeneous underwater channels.These insights contribute to developing more efficient and reliable UCL designs with military standards by enhancing UWOC system performance over a longer linkspan for a given limited optical power across various underwater environments.展开更多
In this study,the interaction between deformation and precipitates during multiple equal channel angular pressing(ECAP)deformations and inter-pass aging combination and its effect on the mechanical properties of 7050 ...In this study,the interaction between deformation and precipitates during multiple equal channel angular pressing(ECAP)deformations and inter-pass aging combination and its effect on the mechanical properties of 7050 aluminum alloy are studied.The result show that ECAP induces numerous substructures and dislocations,effectively promoting the precipitation of theηʹphase exhibiting a bimodal structure during inter-pass aging.Following inter-pass aging and subsequent ECAP,the decrease in grain size(4.8μm)is together with the increase in dislocation density(1.24×10^(15) m^(−2))due to the pinning effect of the precipitated phase.Simultaneously,the dislocation motion causes the second phase particles to become even finer and more diffuse.The synergistic effects of precipitation strengthening,fine grain strengthening,and dislocation strengthening collectively enhance the high strength of aluminum alloys,with ultimate tensile strength and yield strength reaching approximately 610 and 565 MPa,respectively.Meanwhile,ductility remains largely unchanged,primarily due to coordinated grain boundary sliding and the uniform and fine dispersion of second phase particles.展开更多
It is of great significance to study the corrosion process of aluminum(Al)alloys fasteners in order to mitigate corrosion for their widespread applications.In this paper,a method for enhancing the corrosion resistance...It is of great significance to study the corrosion process of aluminum(Al)alloys fasteners in order to mitigate corrosion for their widespread applications.In this paper,a method for enhancing the corrosion resistance of Al alloy fasteners is proposed.7075 Al alloy parts with a fine-grained microstructure were prepared by pre-heat treatment(PHT),combined subsequent equal channel angular pressing(ECAP)and cold upsetting(CU).The corrosion behavior of the specimens was investigated by intergranular corrosion and electrochemical test.Microstructure investigations were carried out by field emission scanning electron microscopy,energy dispersive spectrometer and transmission electron microscopy.The relationship between microstructural evolution and corrosion resistance changes was also explored.The results show that both PHT and ECAP-CU significantly improved the corrosion resistance of the samples and modified the corrosion process.The open circuit potential,corrosion current density and corrosion rate of the alloy on electrochemical test were(-0.812±8.854)×10^(-5) V(vs.SCE),(6.379±0.025)×10^(-6) A/cm^(2) and 0.066 mm/year,respectively,and the intergranular corrosion depth was(557±8)μm.The main factor controlling the corrosion behavior was the microstructure evolution.After PHT,the disappearance of the dendritic structure and the dissolution of the nonequilibrium second phase eliminated the potential difference between the phases,reducing the free energy in the as cast state.When ECAP-CU was used after PHT,the grain refinement was accompanied by a high density of grain boundaries and dislocations,which led to the formation of a denser passivation film on the alloy surface,improving the corrosion resistance in an aggressive environment.展开更多
Hypersonic Glide Vehicles(HGVs)are advanced aircraft that can achieve extremely high speeds(generally over 5 Mach)and maneuverability within the Earth's atmosphere.HGV trajectory prediction is crucial for effectiv...Hypersonic Glide Vehicles(HGVs)are advanced aircraft that can achieve extremely high speeds(generally over 5 Mach)and maneuverability within the Earth's atmosphere.HGV trajectory prediction is crucial for effective defense planning and interception strategies.In recent years,HGV trajectory prediction methods based on deep learning have the great potential to significantly enhance prediction accuracy and efficiency.However,it's still challenging to strike a balance between improving prediction performance and reducing computation costs of the deep learning trajectory prediction models.To solve this problem,we propose a new deep learning framework(FECA-LSMN)for efficient HGV trajectory prediction.The model first uses a Frequency Enhanced Channel Attention(FECA)module to facilitate the fusion of different HGV trajectory features,and then subsequently employs a Light Sampling-oriented Multi-Layer Perceptron Network(LSMN)based on simple MLP-based structures to extract long/shortterm HGV trajectory features for accurate trajectory prediction.Also,we employ a new data normalization method called reversible instance normalization(RevIN)to enhance the prediction accuracy and training stability of the network.Compared to other popular trajectory prediction models based on LSTM,GRU and Transformer,our FECA-LSMN model achieves leading or comparable performance in terms of RMSE,MAE and MAPE metrics while demonstrating notably faster computation time.The ablation experiments show that the incorporation of the FECA module significantly improves the prediction performance of the network.The RevIN data normalization technique outperforms traditional min-max normalization as well.展开更多
The fifth-generation (5G) communication requires a highly accurate estimation of the channel state information (CSI)to take advantage of the massive multiple-input multiple-output(MIMO) system. However, traditional ch...The fifth-generation (5G) communication requires a highly accurate estimation of the channel state information (CSI)to take advantage of the massive multiple-input multiple-output(MIMO) system. However, traditional channel estimation methods do not always yield reliable estimates. The methodology of this paper consists of deep residual shrinkage network (DRSN)neural network-based method that is used to solve this problem.Thus, the channel estimation approach, based on DRSN with its learning ability of noise-containing data, is first introduced. Then,the DRSN is used to train the noise reduction process based on the results of the least square (LS) channel estimation while applying the pilot frequency subcarriers, where the initially estimated subcarrier channel matrix is considered as a three-dimensional tensor of the DRSN input. Afterward, a mixed signal to noise ratio (SNR) training data strategy is proposed based on the learning ability of DRSN under different SNRs. Moreover, a joint mixed scenario training strategy is carried out to test the multi scenarios robustness of DRSN. As for the findings, the numerical results indicate that the DRSN method outperforms the spatial-frequency-temporal convolutional neural networks (SF-CNN)with similar computational complexity and achieves better advantages in the full SNR range than the minimum mean squared error (MMSE) estimator with a limited dataset. Moreover, the DRSN approach shows robustness in different propagation environments.展开更多
In this work,the reflux classifier with closely spaced inclined channels is used as the pre-concentration facility to improve the separation efficiency before the shaking table separation.Three operating parameters of...In this work,the reflux classifier with closely spaced inclined channels is used as the pre-concentration facility to improve the separation efficiency before the shaking table separation.Three operating parameters of reflux classifier(RC)to pre-concentrate fine(0.023−0.15 mm)tailings of antimony oxide were optimized by response surface methodology(RSM)using a three-level Box-Behnken design(BBD).The parameters studied for the optimization were feeding speed,underflow,and ascending water speed.Second-order response functions were produced for the Sb grade and recovery rate of the concentrate.Taking advantage of the quadratic programming,when the factors of feeding,underflow and ascending water are respectively 225,30 and 133 cm^3/min,a better result can be achieved for the concentrate grade of 2.31% and recovery rate of 83.17%.At the same time,70.48% of the tailings with the grade of 0.20% were discarded out of the feeding.The results indicated that the reflux classifier has a good performance in dealing with fine tailings of antimony oxide.Moreover,second-order polynomial equations,ANOVA,and three-dimensional surface plots were developed to evaluate the effects of each parameter on Sb grade and recovery rate of the concentrate.展开更多
The novel closed-form expressions for the average channel capacity of dual selection diversity is presented, as well as, the bit-error rate （BER） of several coherent and noncoherent digital modulation schemes in the...The novel closed-form expressions for the average channel capacity of dual selection diversity is presented, as well as, the bit-error rate （BER） of several coherent and noncoherent digital modulation schemes in the correlated Weibull fading channels with nonidentical statisticS. The results are expressed in terms of Meijer＇s Gfunction, which can be easily evaluated numerically. The simulation results are presented to validate the proposed theoretical analysis and to examine the effects of the fading severity on the concerned quantities.展开更多
Using ethanol or acetone as the working fluid, visualization of oscillations in steady state was observed visually by high-speed cameras, and temperature oscillating and heat transfer characteristics of closed-loop pl...Using ethanol or acetone as the working fluid, visualization of oscillations in steady state was observed visually by high-speed cameras, and temperature oscillating and heat transfer characteristics of closed-loop plate oscillating heat pipe with parallel channels(POHP-PC) were experimentally investigated by varying liquid filled ratios(50%, 70%, 85%), section scales(1 mm×1 mm and 1 mm×1.5 mm), inclination angles, working fluids and heating inputs. It was found that during operating there was mixed flow consisting of plug flow and annular flow in channels of oscillating heat pipe at steady-state. There was an equilibrium position for working fluid of condenser during oscillating, and periodic oscillations occurred up and down in the vicinity of equilibrium position. With heat input increasing, equilibrium position rose slowly as a result of vapor pressure of evaporation.Evaporation temperature oscillating amplitude possessed a trend of small-large-small and frequency trend was of small-large during steady-state. It may be generally concluded that temperature, whether evaporator or condenser, fluctuated sharply or rose continuously when oscillating heat pipe coming to dry burning state. Simultaneously, it was found that temperature difference of cooling water possibly dropped with heat input rising during dry burning state. Thermal resistance of No. 2 with acetone was lower than that of No. 1 during experiments, but No. 2 achieving heat transfer limit was earlier than No. 1. However, with ethanol, thermal resistance of No. 1 and No. 2 were similar with the heating input less than 110-120 W and filling ratios of 50% and 70%. And with filling ratio of 85%, heating transfer performance of No. 2 was better compared to No. 1 during all the experiments.展开更多
An optimal cooperative beamforming for the amplify-and-forward (AF) MIMO two-way relay channels was designed. Supposing the channel state information (CSI) was perfectly known by the receiver and transmitter as well a...An optimal cooperative beamforming for the amplify-and-forward (AF) MIMO two-way relay channels was designed. Supposing the channel state information (CSI) was perfectly known by the receiver and transmitter as well as the relay, optimal beamforming vectors (matrices) of all nodes were jointly designed based on the criterion of minimizing the sum mean square errors (MSMSE). The analysis result shows that the performance effect of transmitting and receiving beamforming pairs is to maximize the receive signal-to-noise ratio (SNR) at two communication nodes, and the rank of the optimal relay beamforming matrix is no larger than two when there is only one data stream at each source node. A simplified algorithm was put forward to accomplish the design based on the analysis conclusions. Simulation results provide that the system performance, which is characterized in terms of bit error rates (BER), is significantly improved by cooperative beamforming, and the performance of the simplified method is not only very close to the optimal one but also with faster iteration speed and much lower computational complexity.展开更多
Matrix perturbation theory is utilized to investigate high-rank line of sight multiple input multiple output channels in a microwave relay system. The upper and lower bounds of channel capacity are derived based on sp...Matrix perturbation theory is utilized to investigate high-rank line of sight multiple input multiple output channels in a microwave relay system. The upper and lower bounds of channel capacity are derived based on space time block codes technique and singular values decomposition. A useful constraint for designing LOS MIMO channels is developed by the use of the condition number of the MIMO channel matrix. The theoretical analysis of channel capacity is confirmed by the simulation. The results show that the proposed method is able to give a physical explanation of the high-rank LOS MIMO channel matrix characteristics.展开更多
The mixed lineage kinase domain-like(MLKL)protein is a key factor in tumor necrosis factor-induced necroptosis.Recent studies on necroptosis execution revealed a commitment role of MLKL in membrane disruption.However,...The mixed lineage kinase domain-like(MLKL)protein is a key factor in tumor necrosis factor-induced necroptosis.Recent studies on necroptosis execution revealed a commitment role of MLKL in membrane disruption.However,our knowledge of how MLKL functions on membrane remains very limited.Here we demonstrate that MLKL forms cation channels that are permeable preferential y to Mg2+rather than Ca2+in the presence of Na+and K+.Moreover,the N-terminal domain containing six helices(H1-H6)is sufficient to form channels.Using the substituted cysteine accessibility method,we further determine that helix H1,H2,H3,H5 and H6 are transmembrane segments,while H4 is located in the cytoplasm.Finally,MLKL-induced membrane depolarization and cell death exhibit a positive correlation to its channel activity.The Mg2+-preferred permeability and five transmembrane segment topology distinguish MLKL from previously identified Mg2+-permeable channels and thus establish MLKL as a novel class of cation channels.展开更多
The lattice-reduction （LR） has been developed to im- prove the performance of the zero-forcing （ZF） precoder in multiple input multiple output （MIMO） systems. Under the assumptions of uncorrelated flat fading ch...The lattice-reduction （LR） has been developed to im- prove the performance of the zero-forcing （ZF） precoder in multiple input multiple output （MIMO） systems. Under the assumptions of uncorrelated flat fading channel model and perfect channel state information at the transmitter （CSIT）, an LR-aided ZF precoder is able to collect the full transmit diversity. With the complex Lenstra- Lenstra-Lov^sz （LLL） algorithm and limited feedforward structure, an LR-aided linear minimum-mean-square-error （LMMSE） pre- coder for spatial correlated MIMO channels and imperfect CSIT is proposed to achieve lower bit error rate （BER）. Assuming a time division duplexing （TDD） MIMO system, correlated block flat fad- ing channel and LMMSE uplink channel estimator, it is proved that the proposed LR-aided LMMSE precoder can also obtain the full transmit diversity through an analytical approach. Furthermore, the simulation results show that with the quadrature phase shift keying （QPSK） modulation at the transmitter, the uncoded and coded BERs of the LR-aided LMMSE precoder are lower than that of the traditional LMMSE precoder respectively when Eb-No is greater than 10 dB and 12 dB at all correlation coefficients.展开更多
Major depressive disorder(MDD)is a common neuropsychiatric disorder characterized by diverse symptoms.There are big limitations of clinic medicine which highlighted an urgent and clear need for more efficacious and fa...Major depressive disorder(MDD)is a common neuropsychiatric disorder characterized by diverse symptoms.There are big limitations of clinic medicine which highlighted an urgent and clear need for more efficacious and faster-acting therapeutic agents to treat patients with MDD,especially those who are refractory to the traditional antidepressants.In the present study,we assessed a novel compound,YY-21,from timosaponin B-Ⅲ derived from sarsasapogenin of Anemarrhenae Rhizoma.We found that YY-21 obviously increased presynaptic glutamate release and enhanced long-term synaptic activity within 10 min as determined by excitatory postsynaptic current(EPSC) and field excitatory postsynaptic potential(fEPSP) in medial prefrontal cortex(mPFC) slices.YY-21 demonstrated anxiolytic-like effects following acute administration in animals and reversed the depressivelike and anxiety phenotypes induced by chronic unpredictable mild stress(CMS) with a relatively fast therapeutic onset.Our mechanism research reveals that NMDA receptors and two-pore domain potassium(K2P)(TREK1) channels emerged as new drug targets for faster acting antidepressants.K2 P channels generate leak currents that are responsible the maintenance of resting membrane potential.They are potential targets for the treatment of multiple diseases.Here we identify TKDC,an inhibitor of the TREK subfamily,including TREK1,TREK2 and TRAAK channels.Using TKDC as a chemical probe,a combined study of computations,mutagenesis,and electrophysiology reveal an allosteric ligand-binding site in the extracellular cap of the channels.The molecular dynamics simulations suggest that ligand-induced allosteric conformational transitions cause a blockage of the ion conductive pathway.The identification of the extracellular ligand-binding site is confirmed by the discovery of new inhibitors targeting this site using virtual screening.These results suggest that the extracellular cap of a K2P channel can act as a new allosteric site and may serve as a direct drug target.展开更多
OBJECTIVE To investigate effects of oxymatrine,an alkaloid from Sophora flavescens Ait.,on high-voltage dependent calcium channel and inhibitory neurotransmitter GABA under neuropathic pain condition.METHODS The parti...OBJECTIVE To investigate effects of oxymatrine,an alkaloid from Sophora flavescens Ait.,on high-voltage dependent calcium channel and inhibitory neurotransmitter GABA under neuropathic pain condition.METHODS The partial sciatic nerve ligation(PSNL)was executed on C57/BL6 mice to produce neuropathic pain.Oxymatrine(150 mg·kg-1)was administrated intraperitoneally to PSNL mice.Mechanical hindpaw withdral threshold(MWT)was measured under Von-Frey filament stimulation with up-and-down method.In brain tissue,GABA concentration was measured with ELISA.Change of GABAAreceptor protein expression,N-type calcium channel(Cav2.2)and L-type calcium channel(Cav1.3)protein expressions were detected with Western-blot;intracellular calcium concentration was measured in cultured cortical neurons with Fluo-3/AM fluorescent probe.RESULTS Compared to saline,oxymatrine significantly increased ED50 of MWT on PSNL mice(P<0.05).GABA concentration and GABAAreceptor protein level in brain tissue were decreased in PSNL mice,while administration of oxymatrine increased both GABA concentration and GABAA receptor expression.Intracellular calcium concentration was increased in cultured cortical neurons by oxymatrine treatment,but this phenomenon was not seen under calcium-free condition.Protein expression of Cav2.2,but not Cav1.3,was found to be decreased in the brains of PSNL mice and to be restored to a normal level with oxymatrine administration.CONCLUSION Oxymatrine has analgesic effect on PSNL-induced neuropathic pain in mice.This phenominon relates to the increase of GABA release,GABAAreceptor expression,and also the restoration of expression level of Cav2.2 but not Cav1.3 in brain tissues,which suggesting that Ca2+ flow through Cav2.2 calcium channel may be the key point underlying oxymatrine analgesia.展开更多
The distribution of velocity is one of the basic issues in river dynamics.Based on the experimental data measured by ADV in the flume of State Key Hydraulics Laboratory (SKHL),this paper analyzed the ver- tical distri...The distribution of velocity is one of the basic issues in river dynamics.Based on the experimental data measured by ADV in the flume of State Key Hydraulics Laboratory (SKHL),this paper analyzed the ver- tical distribution of point velocity and the varying law of turbulence intensity in straight mobile compound chan- nel with an asymmetric floodplain.Above certain relative height,the streamwise point velocity follows the loga- rithmic distribution.Below the location,the velocity varies linearly approxim...展开更多
文摘Due to the complex high-temperature characteristics of hydrocarbon fuel,the research on the long-term working process of parallel channel structure under variable working conditions,especially under high heat-mass ratio,has not been systematically carried out.In this paper,the heat transfer and flow characteristics of related high temperature fuels are studied by using typical engine parallel channel structure.Through numeri⁃cal simulation and systematic experimental verification,the flow and heat transfer characteristics of parallel chan⁃nels under typical working conditions are obtained,and the effectiveness of high-precision calculation method is preliminarily established.It is known that the stable time required for hot start of regenerative cooling engine is about 50 s,and the flow resistance of parallel channel structure first increases and then decreases with the in⁃crease of equivalence ratio(The following equivalence ratio is expressed byΦ),and there is a flow resistance peak in the range ofΦ=0.5~0.8.This is mainly caused by the coupling effect of high temperature physical proper⁃ties,flow rate and pressure of fuel in parallel channels.At the same time,the cooling and heat transfer character⁃istics of parallel channels under some conditions of high heat-mass ratio are obtained,and the main factors affect⁃ing the heat transfer of parallel channels such as improving surface roughness and strengthening heat transfer are mastered.In the experiment,whenΦis less than 0.9,the phenomenon of local heat transfer enhancement and deterioration can be obviously observed,and the temperature rise of local structures exceeds 200℃,which is the risk of structural damage.Therefore,the reliability of long-term parallel channel structure under the condition of high heat-mass ratio should be fully considered in structural design.
文摘In this study,a self-calibrating near-infrared fluorescence probe was designed and synthesized based on the dual-fluorophore strategy utilizing methylene blue and coumarin.The probe utilized methylene blue(emission spectrum range:640-740 nm)and coumarin fluorophore(emission spectrum range:440-600 nm)as signal output units,thereby achieving effective spectral separation and highly selective detection of HClO.Under physiological pH conditions,HClO triggers an oxidation-cleavage reaction,releasing methylene blue and coumarin,which emit distinct red and green fluorescence,respectively.This dual-emission feature enabled rapid HClO detection with two-channel detection limits of 25.13 nmol·L^(-1)(green channel)and 31.55 nmol·L^(-1)(red channel).Furthermore,in cell imaging experiments,this probe demonstrated excellent cell membrane permeability and low cytotoxicity,successfully enabling the monitoring of both endogenous and exogenous HClO in living cells.By incorporating a twochannel self-calibration system,the probe effectively mitigated signal variations caused by instrumental or environmental interference,substantially improving detection sensitivity and reliability.
基金supported by the Education and Teaching Research Project of Universities in Fujian Province(FBJY20230167).
文摘The work takes a new liquid-cooling plate in a power battery with pin fins inside the channel as the object.A mathematical model is established via the central composite design of the response surface to study the relationships among the length,width,height,and spacing of pin fins;the maximum temperature and temperature difference of the battery module;and the pressure drop of the liquid-cooling plate.Model accuracy is verified via variance analysis.The new liquid-cooling plate enables the power battery to work within an optimal temperature range.Appropriately increasing the length,width,and height and reducing the spacing of pin fins could reduce the temperature of the power battery module and improve the temperature uniformity.However,the pressure drop of the liquid-cooling plate increases.The structural parameters of the pin fins are optimized to minimize the maximum temperature and the temperature difference of the battery module as well as the pressure drop of the liquid-cooling plate.The errors between the values predicted and actual by the simulation test are 0.58%,4%,and 0.48%,respectively,which further verifies the model accuracy.The results reveal the influence of the structural parameters of the pin fins inside the liquid-cooling plate on its heat dissipation performance and pressure drop characteristics.A theoretical basis is provided for the design of liquid-cooling plates in power batteries and the optimization of structural parameters.
文摘For segmented detectors,surface flatness is critical as it directly influences both energy resolution and image clarity.Additionally,the limited adjustment range of the segmented detectors necessitates precise benchmark construction.This paper proposes an architecture for detecting detector flatness based on channel spectral dispersion.By measuring the dispersion fringes for coplanar adjustment,the final adjustment residual is improved to better than 300 nm.This result validates the feasibility of the proposed technology and provides significant technical support for the development of next-generation large-aperture sky survey equipment.
文摘Potassium-calcium activates channel subfamily N member 3(KCNN3/SK3/KCa2.3)is involved in regulating cellular calcium signaling,muscle contraction and neurotransmitter release.Dysregulation of the KCNN3 channel is associated with the development of various tumors.We use bioinformatics analysis to identify whether KCNN3 regulates the occurrence and development of stomach adenocarcinoma(STAD)as a prognostic target.By analyzing the Human Protein Atlas(HPA)database and The Cancer Genome Atlas(TCGA)database,we found that the protein and mRNA levels of KCNN3 were dramatically reduced in STAD,and TCGA database showed that KCNN3 significantly correlated with the prognosis and clinical features of STAD.In addition,we found that high expression of KCNN3 in STAD reduced the IC 50 of several drugs in STAD cells,suggesting that high expression of KCNN3 correlated with the drug sensitivity of STAD.To investigate the underlying biological mechanism,we identified a potential KCNN3 interaction factor,tumor necrosis factor receptor superfamily member 7(CD27/TNFRSF7),which is expressed at low levels in STAD.RT-qPCR and Western blotting confirmed that KCNN3 and CD27 positively correlated with each other at protein and mRNA levels,and co-immunoprecipitation and immunofluorescence experiments confirmed that the two proteins interact and colocalize in the cytoplasm.Moreover,we confirmed the inhibitory effect of KCNN3 on the proliferation,migration and invasion of human STAD cells in vitro and in vivo through subcutaneous tumorigenesis and cellular experiments.Furthermore,GO/KEGG enrichment analysis showed that KCNN3 was enriched in signaling pathways regulating the immune response and calcium or metal ion transport.Lastly,we verified through cell co-culture,RT-qPCR and CCK8 assays that high expression of KCNN3 can promote the increase of T cell activating factor and the killing effect of T cells on STAD cells.Therefore,our results suggest that KCNN3 is a potential inhibitory factor affecting the occurrence and progression of STAD.
基金The Deanship of Scientific Research(DSR)at King Abdulaziz University(KAU),Jeddah,Saudi Arabia,has funded this project under Grant No.(KEP-PhD:72-130-1443).
文摘The Underwater Communication Link(UCL)is a crucial component of Underwater Wireless Optical Communication(UWOC)systems,requiring optimised design to mitigate the high power attenuation inherent in seawater.To ensure the reliability of an optimal UCL design,it is essential to account for the three primary scattering regimes:forward scattering(FSC),backward scattering(BSC),and isotropic scattering(ISC)in seawater channels.This study introduces a new photon-tracking model based on a discrete equation,facilitating Monte Carlo Simulation(MCS)to evaluate how different scattering regimes influence received photon distribution.Three distinct Scattering Regime Contribution Weight(SRCW)probability sets were employed,each representing different UCL operational configurations dominated by specific scattering regimes.The proposed modeling approach enables a comprehensive assessment of the temporal characteristics of received optical pulses,channel loss,and time spread-ultimately defining the optimal UCL design parameters.The key findings of this study include:(1)Enhancing the FSC regime dominance leads to a quasi-light waveguide effect over link spans and small Fields of View(FOV)<25°,significantly improving channel performance in Harbor seawater compared to Coastal seawater.(2)A well-designed UCL with a small FOV(<25°)can minimise channel loss and time spread,ensuring high capacity and efficient performance in both Coastal and Harbor seawaters.(3)When BSC and ISC contributions exceed FSC dominance,the received optical pulse undergoes significant temporal broadening,particularly for larger FOV angles(>25°)and extended link spans.(4)The developed novel MCS-based discrete equation provides a simple yet robust model for simulating photon propagation in both homogeneous and inhomogeneous underwater channels.These insights contribute to developing more efficient and reliable UCL designs with military standards by enhancing UWOC system performance over a longer linkspan for a given limited optical power across various underwater environments.
基金Project(52275350)supported by the National Natural Science Foundation of ChinaProject(0301006)supported by the International Cooperative Scientific Research Platform of SUES,China。
文摘In this study,the interaction between deformation and precipitates during multiple equal channel angular pressing(ECAP)deformations and inter-pass aging combination and its effect on the mechanical properties of 7050 aluminum alloy are studied.The result show that ECAP induces numerous substructures and dislocations,effectively promoting the precipitation of theηʹphase exhibiting a bimodal structure during inter-pass aging.Following inter-pass aging and subsequent ECAP,the decrease in grain size(4.8μm)is together with the increase in dislocation density(1.24×10^(15) m^(−2))due to the pinning effect of the precipitated phase.Simultaneously,the dislocation motion causes the second phase particles to become even finer and more diffuse.The synergistic effects of precipitation strengthening,fine grain strengthening,and dislocation strengthening collectively enhance the high strength of aluminum alloys,with ultimate tensile strength and yield strength reaching approximately 610 and 565 MPa,respectively.Meanwhile,ductility remains largely unchanged,primarily due to coordinated grain boundary sliding and the uniform and fine dispersion of second phase particles.
基金Project(52275350)supported by the National Natural Science Foundation of ChinaProject(0301006)supported by International Cooperative Scientific Research Platform of SUES,China。
文摘It is of great significance to study the corrosion process of aluminum(Al)alloys fasteners in order to mitigate corrosion for their widespread applications.In this paper,a method for enhancing the corrosion resistance of Al alloy fasteners is proposed.7075 Al alloy parts with a fine-grained microstructure were prepared by pre-heat treatment(PHT),combined subsequent equal channel angular pressing(ECAP)and cold upsetting(CU).The corrosion behavior of the specimens was investigated by intergranular corrosion and electrochemical test.Microstructure investigations were carried out by field emission scanning electron microscopy,energy dispersive spectrometer and transmission electron microscopy.The relationship between microstructural evolution and corrosion resistance changes was also explored.The results show that both PHT and ECAP-CU significantly improved the corrosion resistance of the samples and modified the corrosion process.The open circuit potential,corrosion current density and corrosion rate of the alloy on electrochemical test were(-0.812±8.854)×10^(-5) V(vs.SCE),(6.379±0.025)×10^(-6) A/cm^(2) and 0.066 mm/year,respectively,and the intergranular corrosion depth was(557±8)μm.The main factor controlling the corrosion behavior was the microstructure evolution.After PHT,the disappearance of the dendritic structure and the dissolution of the nonequilibrium second phase eliminated the potential difference between the phases,reducing the free energy in the as cast state.When ECAP-CU was used after PHT,the grain refinement was accompanied by a high density of grain boundaries and dislocations,which led to the formation of a denser passivation film on the alloy surface,improving the corrosion resistance in an aggressive environment.
文摘Hypersonic Glide Vehicles(HGVs)are advanced aircraft that can achieve extremely high speeds(generally over 5 Mach)and maneuverability within the Earth's atmosphere.HGV trajectory prediction is crucial for effective defense planning and interception strategies.In recent years,HGV trajectory prediction methods based on deep learning have the great potential to significantly enhance prediction accuracy and efficiency.However,it's still challenging to strike a balance between improving prediction performance and reducing computation costs of the deep learning trajectory prediction models.To solve this problem,we propose a new deep learning framework(FECA-LSMN)for efficient HGV trajectory prediction.The model first uses a Frequency Enhanced Channel Attention(FECA)module to facilitate the fusion of different HGV trajectory features,and then subsequently employs a Light Sampling-oriented Multi-Layer Perceptron Network(LSMN)based on simple MLP-based structures to extract long/shortterm HGV trajectory features for accurate trajectory prediction.Also,we employ a new data normalization method called reversible instance normalization(RevIN)to enhance the prediction accuracy and training stability of the network.Compared to other popular trajectory prediction models based on LSTM,GRU and Transformer,our FECA-LSMN model achieves leading or comparable performance in terms of RMSE,MAE and MAPE metrics while demonstrating notably faster computation time.The ablation experiments show that the incorporation of the FECA module significantly improves the prediction performance of the network.The RevIN data normalization technique outperforms traditional min-max normalization as well.
基金supported by the National Key Scientific Instrument and Equipment Development Project(61827801).
文摘The fifth-generation (5G) communication requires a highly accurate estimation of the channel state information (CSI)to take advantage of the massive multiple-input multiple-output(MIMO) system. However, traditional channel estimation methods do not always yield reliable estimates. The methodology of this paper consists of deep residual shrinkage network (DRSN)neural network-based method that is used to solve this problem.Thus, the channel estimation approach, based on DRSN with its learning ability of noise-containing data, is first introduced. Then,the DRSN is used to train the noise reduction process based on the results of the least square (LS) channel estimation while applying the pilot frequency subcarriers, where the initially estimated subcarrier channel matrix is considered as a three-dimensional tensor of the DRSN input. Afterward, a mixed signal to noise ratio (SNR) training data strategy is proposed based on the learning ability of DRSN under different SNRs. Moreover, a joint mixed scenario training strategy is carried out to test the multi scenarios robustness of DRSN. As for the findings, the numerical results indicate that the DRSN method outperforms the spatial-frequency-temporal convolutional neural networks (SF-CNN)with similar computational complexity and achieves better advantages in the full SNR range than the minimum mean squared error (MMSE) estimator with a limited dataset. Moreover, the DRSN approach shows robustness in different propagation environments.
基金Project(2015SK20792)supported by Key Province Key Technology Research and Development Program of the Ministry of Science and Technology of Hunan,ChinaProjects(2019zzts703,2020zzts740,2020zzts202)supported by the Fundamental Research Funds for the Central Universities of ChinaProject(2020P4FZG03A)supported by State Key Laboratory of Vanadium and Titanium Resources Comprehensive Utilization,China。
文摘In this work,the reflux classifier with closely spaced inclined channels is used as the pre-concentration facility to improve the separation efficiency before the shaking table separation.Three operating parameters of reflux classifier(RC)to pre-concentrate fine(0.023−0.15 mm)tailings of antimony oxide were optimized by response surface methodology(RSM)using a three-level Box-Behnken design(BBD).The parameters studied for the optimization were feeding speed,underflow,and ascending water speed.Second-order response functions were produced for the Sb grade and recovery rate of the concentrate.Taking advantage of the quadratic programming,when the factors of feeding,underflow and ascending water are respectively 225,30 and 133 cm^3/min,a better result can be achieved for the concentrate grade of 2.31% and recovery rate of 83.17%.At the same time,70.48% of the tailings with the grade of 0.20% were discarded out of the feeding.The results indicated that the reflux classifier has a good performance in dealing with fine tailings of antimony oxide.Moreover,second-order polynomial equations,ANOVA,and three-dimensional surface plots were developed to evaluate the effects of each parameter on Sb grade and recovery rate of the concentrate.
基金the National High-Tech Research and Development Program (2002AA123032)the Innovative Research Team Program of UESTC, China.
文摘The novel closed-form expressions for the average channel capacity of dual selection diversity is presented, as well as, the bit-error rate （BER） of several coherent and noncoherent digital modulation schemes in the correlated Weibull fading channels with nonidentical statisticS. The results are expressed in terms of Meijer＇s Gfunction, which can be easily evaluated numerically. The simulation results are presented to validate the proposed theoretical analysis and to examine the effects of the fading severity on the concerned quantities.
基金Project(51306198)supported by the National Natural Science Foundation of ChinaProject(NR2013K07)supported by Beijing Key Lab of Heating,Gas Supply,Ventilating and Air Conditioning Engineering,China+1 种基金Project(331614013)supported by Beijing University of Civil Engineering and Architecture,ChinaProject(00921915023)supported by Organization Department of Beijing,China
文摘Using ethanol or acetone as the working fluid, visualization of oscillations in steady state was observed visually by high-speed cameras, and temperature oscillating and heat transfer characteristics of closed-loop plate oscillating heat pipe with parallel channels(POHP-PC) were experimentally investigated by varying liquid filled ratios(50%, 70%, 85%), section scales(1 mm×1 mm and 1 mm×1.5 mm), inclination angles, working fluids and heating inputs. It was found that during operating there was mixed flow consisting of plug flow and annular flow in channels of oscillating heat pipe at steady-state. There was an equilibrium position for working fluid of condenser during oscillating, and periodic oscillations occurred up and down in the vicinity of equilibrium position. With heat input increasing, equilibrium position rose slowly as a result of vapor pressure of evaporation.Evaporation temperature oscillating amplitude possessed a trend of small-large-small and frequency trend was of small-large during steady-state. It may be generally concluded that temperature, whether evaporator or condenser, fluctuated sharply or rose continuously when oscillating heat pipe coming to dry burning state. Simultaneously, it was found that temperature difference of cooling water possibly dropped with heat input rising during dry burning state. Thermal resistance of No. 2 with acetone was lower than that of No. 1 during experiments, but No. 2 achieving heat transfer limit was earlier than No. 1. However, with ethanol, thermal resistance of No. 1 and No. 2 were similar with the heating input less than 110-120 W and filling ratios of 50% and 70%. And with filling ratio of 85%, heating transfer performance of No. 2 was better compared to No. 1 during all the experiments.
基金Project(60902092)supported by the National Natural Science Foundation of China
文摘An optimal cooperative beamforming for the amplify-and-forward (AF) MIMO two-way relay channels was designed. Supposing the channel state information (CSI) was perfectly known by the receiver and transmitter as well as the relay, optimal beamforming vectors (matrices) of all nodes were jointly designed based on the criterion of minimizing the sum mean square errors (MSMSE). The analysis result shows that the performance effect of transmitting and receiving beamforming pairs is to maximize the receive signal-to-noise ratio (SNR) at two communication nodes, and the rank of the optimal relay beamforming matrix is no larger than two when there is only one data stream at each source node. A simplified algorithm was put forward to accomplish the design based on the analysis conclusions. Simulation results provide that the system performance, which is characterized in terms of bit error rates (BER), is significantly improved by cooperative beamforming, and the performance of the simplified method is not only very close to the optimal one but also with faster iteration speed and much lower computational complexity.
基金supported partly by the National Natural Science Foundation of China(60872022)the"973"Program of China(2008CB317109),the Science Foundation of Guangxi Province of China(0991241)the Foundation of Guangxi Key Laboratory of Information and Communication(10903).
文摘Matrix perturbation theory is utilized to investigate high-rank line of sight multiple input multiple output channels in a microwave relay system. The upper and lower bounds of channel capacity are derived based on space time block codes technique and singular values decomposition. A useful constraint for designing LOS MIMO channels is developed by the use of the condition number of the MIMO channel matrix. The theoretical analysis of channel capacity is confirmed by the simulation. The results show that the proposed method is able to give a physical explanation of the high-rank LOS MIMO channel matrix characteristics.
文摘The mixed lineage kinase domain-like(MLKL)protein is a key factor in tumor necrosis factor-induced necroptosis.Recent studies on necroptosis execution revealed a commitment role of MLKL in membrane disruption.However,our knowledge of how MLKL functions on membrane remains very limited.Here we demonstrate that MLKL forms cation channels that are permeable preferential y to Mg2+rather than Ca2+in the presence of Na+and K+.Moreover,the N-terminal domain containing six helices(H1-H6)is sufficient to form channels.Using the substituted cysteine accessibility method,we further determine that helix H1,H2,H3,H5 and H6 are transmembrane segments,while H4 is located in the cytoplasm.Finally,MLKL-induced membrane depolarization and cell death exhibit a positive correlation to its channel activity.The Mg2+-preferred permeability and five transmembrane segment topology distinguish MLKL from previously identified Mg2+-permeable channels and thus establish MLKL as a novel class of cation channels.
基金supported by the National Science Fund for Distinguished Young Scholars (60725105)the National Basic Research Program of China (2009CB320404)+4 种基金the Program for Changjiang Scholars and Innovative Research Team in University (IRT0852)the 111 Project(B08038)the National Natural Science Foundation of China (60702057)the Special Research Fund of State Key Laboratory (ISN1102003)the National Science and Technology Major Project (2011ZX03001-007-01)
文摘The lattice-reduction （LR） has been developed to im- prove the performance of the zero-forcing （ZF） precoder in multiple input multiple output （MIMO） systems. Under the assumptions of uncorrelated flat fading channel model and perfect channel state information at the transmitter （CSIT）, an LR-aided ZF precoder is able to collect the full transmit diversity. With the complex Lenstra- Lenstra-Lov^sz （LLL） algorithm and limited feedforward structure, an LR-aided linear minimum-mean-square-error （LMMSE） pre- coder for spatial correlated MIMO channels and imperfect CSIT is proposed to achieve lower bit error rate （BER）. Assuming a time division duplexing （TDD） MIMO system, correlated block flat fad- ing channel and LMMSE uplink channel estimator, it is proved that the proposed LR-aided LMMSE precoder can also obtain the full transmit diversity through an analytical approach. Furthermore, the simulation results show that with the quadrature phase shift keying （QPSK） modulation at the transmitter, the uncoded and coded BERs of the LR-aided LMMSE precoder are lower than that of the traditional LMMSE precoder respectively when Eb-No is greater than 10 dB and 12 dB at all correlation coefficients.
文摘Major depressive disorder(MDD)is a common neuropsychiatric disorder characterized by diverse symptoms.There are big limitations of clinic medicine which highlighted an urgent and clear need for more efficacious and faster-acting therapeutic agents to treat patients with MDD,especially those who are refractory to the traditional antidepressants.In the present study,we assessed a novel compound,YY-21,from timosaponin B-Ⅲ derived from sarsasapogenin of Anemarrhenae Rhizoma.We found that YY-21 obviously increased presynaptic glutamate release and enhanced long-term synaptic activity within 10 min as determined by excitatory postsynaptic current(EPSC) and field excitatory postsynaptic potential(fEPSP) in medial prefrontal cortex(mPFC) slices.YY-21 demonstrated anxiolytic-like effects following acute administration in animals and reversed the depressivelike and anxiety phenotypes induced by chronic unpredictable mild stress(CMS) with a relatively fast therapeutic onset.Our mechanism research reveals that NMDA receptors and two-pore domain potassium(K2P)(TREK1) channels emerged as new drug targets for faster acting antidepressants.K2 P channels generate leak currents that are responsible the maintenance of resting membrane potential.They are potential targets for the treatment of multiple diseases.Here we identify TKDC,an inhibitor of the TREK subfamily,including TREK1,TREK2 and TRAAK channels.Using TKDC as a chemical probe,a combined study of computations,mutagenesis,and electrophysiology reveal an allosteric ligand-binding site in the extracellular cap of the channels.The molecular dynamics simulations suggest that ligand-induced allosteric conformational transitions cause a blockage of the ion conductive pathway.The identification of the extracellular ligand-binding site is confirmed by the discovery of new inhibitors targeting this site using virtual screening.These results suggest that the extracellular cap of a K2P channel can act as a new allosteric site and may serve as a direct drug target.
基金The project supported by National Natural Science Foundation of China(81102901)Scientific Research Foundation for Returned Chinese Scholars([2012]940)Natural Science Foundation of Liaoning Province(201102151)
文摘OBJECTIVE To investigate effects of oxymatrine,an alkaloid from Sophora flavescens Ait.,on high-voltage dependent calcium channel and inhibitory neurotransmitter GABA under neuropathic pain condition.METHODS The partial sciatic nerve ligation(PSNL)was executed on C57/BL6 mice to produce neuropathic pain.Oxymatrine(150 mg·kg-1)was administrated intraperitoneally to PSNL mice.Mechanical hindpaw withdral threshold(MWT)was measured under Von-Frey filament stimulation with up-and-down method.In brain tissue,GABA concentration was measured with ELISA.Change of GABAAreceptor protein expression,N-type calcium channel(Cav2.2)and L-type calcium channel(Cav1.3)protein expressions were detected with Western-blot;intracellular calcium concentration was measured in cultured cortical neurons with Fluo-3/AM fluorescent probe.RESULTS Compared to saline,oxymatrine significantly increased ED50 of MWT on PSNL mice(P<0.05).GABA concentration and GABAAreceptor protein level in brain tissue were decreased in PSNL mice,while administration of oxymatrine increased both GABA concentration and GABAA receptor expression.Intracellular calcium concentration was increased in cultured cortical neurons by oxymatrine treatment,but this phenomenon was not seen under calcium-free condition.Protein expression of Cav2.2,but not Cav1.3,was found to be decreased in the brains of PSNL mice and to be restored to a normal level with oxymatrine administration.CONCLUSION Oxymatrine has analgesic effect on PSNL-induced neuropathic pain in mice.This phenominon relates to the increase of GABA release,GABAAreceptor expression,and also the restoration of expression level of Cav2.2 but not Cav1.3 in brain tissues,which suggesting that Ca2+ flow through Cav2.2 calcium channel may be the key point underlying oxymatrine analgesia.
基金Supported by Key Project of Chinese.Ministry of Education (03134)
文摘The distribution of velocity is one of the basic issues in river dynamics.Based on the experimental data measured by ADV in the flume of State Key Hydraulics Laboratory (SKHL),this paper analyzed the ver- tical distribution of point velocity and the varying law of turbulence intensity in straight mobile compound chan- nel with an asymmetric floodplain.Above certain relative height,the streamwise point velocity follows the loga- rithmic distribution.Below the location,the velocity varies linearly approxim...
