Sequential ultrasonograms of the ccrvix and uterus were taken in both cross and longitudinal sections at different periods of involution in eleven postparturn Jersey cows, and in the meantime the concentrations of ...Sequential ultrasonograms of the ccrvix and uterus were taken in both cross and longitudinal sections at different periods of involution in eleven postparturn Jersey cows, and in the meantime the concentrations of ocstndiol-17β and progesterone in peripheral circulation of the cows were measured by radioimmunoassay(RIA). Cervical involution was showed in detail by using ultrasound scanner during its whole involuting phase, and the oestrus could be indicated by the differences of the ultrasonograms of the genital tract at the time. It took 46.7+3.9 days for the cervix to complete involution after calving, and its involuting rate was delayed by the occurence of the periparturient fatty liver and injury of the cervix during parturtion.展开更多
Objective To observe the cervical elasticity of healthy adult nulliparous women at different age groups and different stages of menstrual cycle with E-Cervix imaging technology.Methods A total of 218 healthy adult nul...Objective To observe the cervical elasticity of healthy adult nulliparous women at different age groups and different stages of menstrual cycle with E-Cervix imaging technology.Methods A total of 218 healthy adult nulliparous women who underwent transvaginal ultrasound examination for routine physical examination were retrospectively enrolled,including 103 in follicular phase,78 in ovulation phase and 37 in luteal phase.Cervical canal length(CL)and E-Cervix elasticity parameters were compared among different age groups and different stages of menstrual cycle,including elasticity contrast index(ECI),hardness ratio(HR),cervical internal and external orifice strain values(IOS and EOS)and IOS/EOS ratio.Results No significant difference of CL nor cervical elasticity parameters was detected among healthy adult nulliparous women at different age groups(all P>0.05).There were significant differences of ECI,HR and IOS among different menstrual cycle stages(all P<0.05),among which women in follicular phase had higher ECI and IOS but lower HR than those in luteal phase(all P<0.05).Conclusion No significant difference of cervical elasticity existed among healthy adult nulliparous women at different age groups.Meanwhile,cervical elasticity of healthy adult nulliparous women changed during menstrual cycle,in follicular phase had higher ECI and IOS but lower HR than in luteal phase.展开更多
目的:通过原位杂交检测技术探索高危型人乳头瘤病毒(HR-HPV)分型检测阴性的子宫颈高级别鳞状上皮内病变(HSIL)是否由人乳头瘤病毒(HPV)感染引起,进而为子宫颈HSIL的精确诊断及治疗提供新思路。方法:收集2020年1月至2022年10月就诊于山...目的:通过原位杂交检测技术探索高危型人乳头瘤病毒(HR-HPV)分型检测阴性的子宫颈高级别鳞状上皮内病变(HSIL)是否由人乳头瘤病毒(HPV)感染引起,进而为子宫颈HSIL的精确诊断及治疗提供新思路。方法:收集2020年1月至2022年10月就诊于山东第二医科大学附属医院妇科行子宫颈活检或手术治疗的305例子宫颈HSIL患者的病历资料,其中HR-HPV分型检测阴性患者为研究组(15例),阳性患者为对照组(290例)。采用RNAscope原位杂交技术检测两组患者样本中HPV E6/E7 m RNA的表达,并使用染色强度半定量评分分析HPV E6/E7 m RNA的表达情况,同时采用免疫组织化学(IHC)方法检测p16、Ki-67的表达情况。结果:(1)305例HSIL患者中,15例(4.9%)HR-HPV分型检测为阴性。(2)研究组中73.3%(11/15)HPV E6/E7 m RNA呈阳性表达,与对照组100.0%(290/290)比较,差异有统计学意义(P<0.001)。(3)研究组p16的阳性表达率与对照组比较,差异有统计学意义(93.3%vs.100.0%,P=0.049),两组Ki-67阳性表达率均为100.0%。(4)组织病理学类型为子宫颈上皮内瘤变(CIN)2的样本中,研究组HPV E6/E7 m RNA总体评分明显低于对照组,差异有统计学意义(P=0.004);CIN3的样本中,研究组HPV E6/E7 m RNA总体评分虽低于对照组,但差异无统计学意义(P=0.158)。结论:子宫颈癌筛查中,单纯采用HR-HPV分型检测可能导致4.9%的HSIL患者因结果阴性而出现漏诊或误诊。HPV E6/E7 m RNA原位杂交检测技术可能有助于降低HR-HPV分型检测在HSIL中的假阴性率,并辅助预测子宫颈病变的发展方向,进而为实现个体化精准治疗提供新的思路。展开更多
文摘Sequential ultrasonograms of the ccrvix and uterus were taken in both cross and longitudinal sections at different periods of involution in eleven postparturn Jersey cows, and in the meantime the concentrations of ocstndiol-17β and progesterone in peripheral circulation of the cows were measured by radioimmunoassay(RIA). Cervical involution was showed in detail by using ultrasound scanner during its whole involuting phase, and the oestrus could be indicated by the differences of the ultrasonograms of the genital tract at the time. It took 46.7+3.9 days for the cervix to complete involution after calving, and its involuting rate was delayed by the occurence of the periparturient fatty liver and injury of the cervix during parturtion.
文摘Objective To observe the cervical elasticity of healthy adult nulliparous women at different age groups and different stages of menstrual cycle with E-Cervix imaging technology.Methods A total of 218 healthy adult nulliparous women who underwent transvaginal ultrasound examination for routine physical examination were retrospectively enrolled,including 103 in follicular phase,78 in ovulation phase and 37 in luteal phase.Cervical canal length(CL)and E-Cervix elasticity parameters were compared among different age groups and different stages of menstrual cycle,including elasticity contrast index(ECI),hardness ratio(HR),cervical internal and external orifice strain values(IOS and EOS)and IOS/EOS ratio.Results No significant difference of CL nor cervical elasticity parameters was detected among healthy adult nulliparous women at different age groups(all P>0.05).There were significant differences of ECI,HR and IOS among different menstrual cycle stages(all P<0.05),among which women in follicular phase had higher ECI and IOS but lower HR than those in luteal phase(all P<0.05).Conclusion No significant difference of cervical elasticity existed among healthy adult nulliparous women at different age groups.Meanwhile,cervical elasticity of healthy adult nulliparous women changed during menstrual cycle,in follicular phase had higher ECI and IOS but lower HR than in luteal phase.
文摘目的:通过原位杂交检测技术探索高危型人乳头瘤病毒(HR-HPV)分型检测阴性的子宫颈高级别鳞状上皮内病变(HSIL)是否由人乳头瘤病毒(HPV)感染引起,进而为子宫颈HSIL的精确诊断及治疗提供新思路。方法:收集2020年1月至2022年10月就诊于山东第二医科大学附属医院妇科行子宫颈活检或手术治疗的305例子宫颈HSIL患者的病历资料,其中HR-HPV分型检测阴性患者为研究组(15例),阳性患者为对照组(290例)。采用RNAscope原位杂交技术检测两组患者样本中HPV E6/E7 m RNA的表达,并使用染色强度半定量评分分析HPV E6/E7 m RNA的表达情况,同时采用免疫组织化学(IHC)方法检测p16、Ki-67的表达情况。结果:(1)305例HSIL患者中,15例(4.9%)HR-HPV分型检测为阴性。(2)研究组中73.3%(11/15)HPV E6/E7 m RNA呈阳性表达,与对照组100.0%(290/290)比较,差异有统计学意义(P<0.001)。(3)研究组p16的阳性表达率与对照组比较,差异有统计学意义(93.3%vs.100.0%,P=0.049),两组Ki-67阳性表达率均为100.0%。(4)组织病理学类型为子宫颈上皮内瘤变(CIN)2的样本中,研究组HPV E6/E7 m RNA总体评分明显低于对照组,差异有统计学意义(P=0.004);CIN3的样本中,研究组HPV E6/E7 m RNA总体评分虽低于对照组,但差异无统计学意义(P=0.158)。结论:子宫颈癌筛查中,单纯采用HR-HPV分型检测可能导致4.9%的HSIL患者因结果阴性而出现漏诊或误诊。HPV E6/E7 m RNA原位杂交检测技术可能有助于降低HR-HPV分型检测在HSIL中的假阴性率,并辅助预测子宫颈病变的发展方向,进而为实现个体化精准治疗提供新的思路。
