Peaches are subject to flesh softening during postharvest storage and transport,which affects the storage life of the fruit and causes huge economic losses.Previous research has demonstrated that postharvest brassinol...Peaches are subject to flesh softening during postharvest storage and transport,which affects the storage life of the fruit and causes huge economic losses.Previous research has demonstrated that postharvest brassinolide treatment can maintain flesh firmness,ascorbic acid and soluble solids contents,and enhance disease resistance in peach fruits.This study assessed the influence of postharvest brassinolide treatment on the expression of key genes involved in cell wall degradation and the phenylpropanoid pathway in peach fruits by real-time fluorescence quantitative polymerase chain reaction(qPCR).The results showed that brassinolide dipping inhibited the gene expression of pectate lyase 1,polygalacturonase 21 and pectin methylesterase 1,and significantly enhanced the gene expression of peroxidase,cinnamoyl-CoA reductase,phenylalanine ammonia lyase and caffeoyl-CoA-O-methyltransferase 5 in peach fruits.It also increased the gene expression levels of chaleone synthase,chaleone isomerase,dihydroflavonol-4-reductase and flavanone 3-hydroxylase at the early stage of storage.These findings imply that brassinolide can suppress the expression of key genes involved in cell wall degradation and enhance the expression of key genes involved in the phenylpropanoid pathway,thereby delaying peach fruit softening and enhancing disease resistance.展开更多
A distinct set of homoeologous cellulose synthase catalytic subunit(CesA) genes are coordinately up-regulated with the onset of secondary wall formation in cotton fiber as shown by quantitative-RT-
Species of the fungus Trichoderma, a genus of Hyphomycetes, are ubiquitous in the environment, but especially in soil. They have been used in a wide range of commercial applications including the production of hydrola...Species of the fungus Trichoderma, a genus of Hyphomycetes, are ubiquitous in the environment, but especially in soil. They have been used in a wide range of commercial applications including the production of hydrolases and in the biological control of plant diseases. A fundamental part of the Trichoderma antifungal system consists of a series of genes coding for a surprising variety of extracellular cell wall degrading enzymes (CWDE). Characterisation and identification of strains at the species level is the first step in utilizing the full potential of fungi in specific applications. One aim when isolating Trichoderma strains is to identify those which can be used in new agricultural and industrial applications. In the past it was not uncommon that biocontrol strains were defined as T. harzianum Rifai, due to the limited classification system of the genus Trichoderma. In recent years, several PCR-based molecular techniques have been used to detect and discriminate among microorganisms. Sequence analysis of the ITS regions of the ribosomal DNA and gene fragments as those corresponding to tef1 gene have been helpful in the neotypification, description and characterization of species in the genus Trichoderma. Another useful method for the identification of Trichoderma strains is the randomly amplified polymorphic DNA (RAPD) technique. Isozyme polymorphisms evaluation of five putative extracellular lytic enzymes loci (β-1,3-glucanase, β-1,6-glucanase, cellulase, chitinase and protease antivities) were carried out using representative strains of defined molecular groups. CWDE groupings obtained from biocontrol strains are discussed in relation to their phylogenetic location and antifungal activities. Compiling morphological, biochemical and sequence information data into a common database would provide a useful resource that could be used to accurately name new haplotypes identified in the future and correctly place them within the genus Trichoderma.展开更多
文摘Peaches are subject to flesh softening during postharvest storage and transport,which affects the storage life of the fruit and causes huge economic losses.Previous research has demonstrated that postharvest brassinolide treatment can maintain flesh firmness,ascorbic acid and soluble solids contents,and enhance disease resistance in peach fruits.This study assessed the influence of postharvest brassinolide treatment on the expression of key genes involved in cell wall degradation and the phenylpropanoid pathway in peach fruits by real-time fluorescence quantitative polymerase chain reaction(qPCR).The results showed that brassinolide dipping inhibited the gene expression of pectate lyase 1,polygalacturonase 21 and pectin methylesterase 1,and significantly enhanced the gene expression of peroxidase,cinnamoyl-CoA reductase,phenylalanine ammonia lyase and caffeoyl-CoA-O-methyltransferase 5 in peach fruits.It also increased the gene expression levels of chaleone synthase,chaleone isomerase,dihydroflavonol-4-reductase and flavanone 3-hydroxylase at the early stage of storage.These findings imply that brassinolide can suppress the expression of key genes involved in cell wall degradation and enhance the expression of key genes involved in the phenylpropanoid pathway,thereby delaying peach fruit softening and enhancing disease resistance.
文摘A distinct set of homoeologous cellulose synthase catalytic subunit(CesA) genes are coordinately up-regulated with the onset of secondary wall formation in cotton fiber as shown by quantitative-RT-
文摘Species of the fungus Trichoderma, a genus of Hyphomycetes, are ubiquitous in the environment, but especially in soil. They have been used in a wide range of commercial applications including the production of hydrolases and in the biological control of plant diseases. A fundamental part of the Trichoderma antifungal system consists of a series of genes coding for a surprising variety of extracellular cell wall degrading enzymes (CWDE). Characterisation and identification of strains at the species level is the first step in utilizing the full potential of fungi in specific applications. One aim when isolating Trichoderma strains is to identify those which can be used in new agricultural and industrial applications. In the past it was not uncommon that biocontrol strains were defined as T. harzianum Rifai, due to the limited classification system of the genus Trichoderma. In recent years, several PCR-based molecular techniques have been used to detect and discriminate among microorganisms. Sequence analysis of the ITS regions of the ribosomal DNA and gene fragments as those corresponding to tef1 gene have been helpful in the neotypification, description and characterization of species in the genus Trichoderma. Another useful method for the identification of Trichoderma strains is the randomly amplified polymorphic DNA (RAPD) technique. Isozyme polymorphisms evaluation of five putative extracellular lytic enzymes loci (β-1,3-glucanase, β-1,6-glucanase, cellulase, chitinase and protease antivities) were carried out using representative strains of defined molecular groups. CWDE groupings obtained from biocontrol strains are discussed in relation to their phylogenetic location and antifungal activities. Compiling morphological, biochemical and sequence information data into a common database would provide a useful resource that could be used to accurately name new haplotypes identified in the future and correctly place them within the genus Trichoderma.
