Monocyclic nitrogen-rich 3-(aminomethyl)-4,5-diamine-1,2,4-triazole(1)and fused cyclic 3,7-diamine-6-(aminomethyl)-[1,2,4]triazolo[4,3-b][1,2,4]triazole(9)were synthesized through the convenient cyclization reaction f...Monocyclic nitrogen-rich 3-(aminomethyl)-4,5-diamine-1,2,4-triazole(1)and fused cyclic 3,7-diamine-6-(aminomethyl)-[1,2,4]triazolo[4,3-b][1,2,4]triazole(9)were synthesized through the convenient cyclization reaction from the readily available reactant.Their energetic salts with high nitrogen content were proved to be rare examples of divalent monocyclic/fused cyclic cationic salts according to the single crystal analyses.The structure of intermediate B was also identified and verified by its trivalent cation crystal 17.5H_2O indirectly.Energetic compounds 2-8 and 10-17 were fully characterized by NMR spectroscopy,infrared spectroscopy,differential scanning calorimetry,elemental analysis.These energetic salts exhibit good thermal stability with decomposition temperatures ranged from 182℃to 245℃.The sensitivity of compounds 2,6,10 and 14 is similar or superior to that of RDX while the others were much more insensitive to mechanical stimulate.Furthermore,detonation velocity of 10(8843 m/s)surpass that of RDX(D=8795 m/s).Considering the high gas production volume(≥808 L/kg)of 2,4,10and 12,constant-volume combustion experiments were conduct to evaluate their gas production capacities specifically.These compounds possess much higher maximum gas-production pressures(P_(max):7.88-10.08 MPa)than the commonly used reagent guanidine nitrate(GN:P_(max)=4.20 MPa),which indicate their strong gas production capacity.展开更多
The mixed lineage kinase domain-like(MLKL)protein is a key factor in tumor necrosis factor-induced necroptosis.Recent studies on necroptosis execution revealed a commitment role of MLKL in membrane disruption.However,...The mixed lineage kinase domain-like(MLKL)protein is a key factor in tumor necrosis factor-induced necroptosis.Recent studies on necroptosis execution revealed a commitment role of MLKL in membrane disruption.However,our knowledge of how MLKL functions on membrane remains very limited.Here we demonstrate that MLKL forms cation channels that are permeable preferential y to Mg2+rather than Ca2+in the presence of Na+and K+.Moreover,the N-terminal domain containing six helices(H1-H6)is sufficient to form channels.Using the substituted cysteine accessibility method,we further determine that helix H1,H2,H3,H5 and H6 are transmembrane segments,while H4 is located in the cytoplasm.Finally,MLKL-induced membrane depolarization and cell death exhibit a positive correlation to its channel activity.The Mg2+-preferred permeability and five transmembrane segment topology distinguish MLKL from previously identified Mg2+-permeable channels and thus establish MLKL as a novel class of cation channels.展开更多
In this paper,the author defined the interpretation system of geoparks,studied the designing princeples and methods of the interpretation identification system,including the information,content and appearance. Further...In this paper,the author defined the interpretation system of geoparks,studied the designing princeples and methods of the interpretation identification system,including the information,content and appearance. Furthermore,the designing of the interpretation identification system designing of the Hanas National Geopark was conducted as an empirical study,展开更多
OBJECTIVE To investigate how MLKL functions on the membrane and explore its electrophysiological characters and structure.METHODS The full-length human MLKL were expressed in SF21 cells and purified using glutathione-...OBJECTIVE To investigate how MLKL functions on the membrane and explore its electrophysiological characters and structure.METHODS The full-length human MLKL were expressed in SF21 cells and purified using glutathione-sepharose affinity chromatography.The currents of purified MLKL proteins were recorded in avoltage-clamp mode using a Warner BC-535 bilayer clamp amplifier.The currents were digitized using p CLAMP 10.2 software.HEK293 cells were cultured and transfected with MLKL plasmid.Cell viability was examined using the Cell Titer-Glo Luminescent Cell Viability Assay kit.RESULT MLKL forms cation channels that are permeable preferentially to Mg2+rather than Ca2+in the presence of Na+and K+.Moreover,each MLKL monomer contains five transmembrane helices:H1,H2,H3,H5 and H6 of the N-terminal domain which is sufficient to form channels.Finally,MLKL-induced membrane depolarization and cell death exhibit a positive correlation to its channel activity.展开更多
As a strategy for phytopathogen control throughtransgenic way,antimicrobial peptide genes havebeen employed over the last two decades.CEMA is a cationic antimicrobial chimericpeptide,which is produced by fusing eighta...As a strategy for phytopathogen control throughtransgenic way,antimicrobial peptide genes havebeen employed over the last two decades.CEMA is a cationic antimicrobial chimericpeptide,which is produced by fusing eightamino acid residues from the antimicrobialpeptide ceropin A with a modified meltin.Threetransgenic cotton plants were generated展开更多
The most remarkable effect in spinel ferrites is the strong dependence of properties on the state of structural disorder and,in particular,on the cation distribution.The structural characterization of a Co-Zn ferrite ...The most remarkable effect in spinel ferrites is the strong dependence of properties on the state of structural disorder and,in particular,on the cation distribution.The structural characterization of a Co-Zn ferrite nanoparticle sample was reported which prepared by wet chemical co-precipitation method.The samples were sintered at three different temperatures viz.650℃,850℃ and 1050℃ for 12 h.The structural details like:lattice constant and distribution of cations in the tetrahedral and octahedral interstitial voids have been deduced through X-ray diffraction (XRD) data analysis.Lattice constant was found to increase with the increase in Zn2+ ions and sintering temperature.Theoretical intensity ratios of (220),(400),(440) planes were considered,as these reflections are sensitive to cations on the A and B sites.Close agreement of the theoretical intensity ratio with the intensity ratio observed from XRD pattern supports the occupancy of Zn2+ ions and Co2+ ions on the octahedral and tetrahedral sites,respectively.展开更多
A novel method is proposed for the supervised classification of multifrequency polarimetric synthetic aperture radar （PolSAR） images. The coherency matrices in P-, L-, and C-bands are mapped onto a 9×9 matrix ...A novel method is proposed for the supervised classification of multifrequency polarimetric synthetic aperture radar （PolSAR） images. The coherency matrices in P-, L-, and C-bands are mapped onto a 9×9 matrix Ω based on the eigenvalue decomposition of the coherency matrix of each band. A boxcar filter is then performed on the matrix Ω. The filtered data are put into a complex Wishart classifier. Finally, the effectiveness of the proposed method is demonstrated with JPL/AIRSAR multifrequency PolSAR data acquired over the Flevoland area.展开更多
The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods.It was found that addition of divalent metal ions(Mg^(2+),Ca^(2+),Mn...The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods.It was found that addition of divalent metal ions(Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni^(2+))resulted in further decrease of the ethidium fluorescence intensity,while a small increase was observed in the TMPyP emission band,implying that the energy of excited ethidium was transferred to TMPyP.This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions.Among the divalent metal ions,alkali earth metal ions and Mn^(2+)displayed higher quenching efficiencies than other transition metal ions.The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68,66,62,48and 38in the presence of100μmol·L^(-1 )of Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni ^(2+)ion,respectively.The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.展开更多
This contribution is not only intended to provide a fresh review for researches on static electrification, but also contains a sub- stantial amount of new material with a view of closing some gaps in the present state...This contribution is not only intended to provide a fresh review for researches on static electrification, but also contains a sub- stantial amount of new material with a view of closing some gaps in the present state of knowledge of oil streaming electrification. In this paper, the Electrostatic Charging Tendency (ECT) of fresh (unaged) and aged transformer oils were investigated in a spinning disk system. Static electrification measurement at different aging degrees was carried out under laboratory controlled conditions. Changes in static elec- trification were compared to some classical aging indexes (conductivity, dissipation factor, water content, resistivity, etc.). Fast, inexpensive and reliable laboratory testing procedures developed by American Society for Testing Materials (D 6802 and D 6181) were also used to monitor decay products as trace impurities. The obtained results show that static electrification currents increase with temperature, fluid mo- tion velocity, surface properties and the aging byproducts of the oil. The polarity, the amplitude and the time constant of the electrification currents are also affected. The results show that static electrification has relationship with thermal aging and is sensitive to turbidimetric and spectrophotometric measurements.展开更多
基金supported by the National Natural Science Foundation of China(No.21875110,22075143)the Science Challenge Project(No.TZ2018004)the Qing Lan Project for the grant。
文摘Monocyclic nitrogen-rich 3-(aminomethyl)-4,5-diamine-1,2,4-triazole(1)and fused cyclic 3,7-diamine-6-(aminomethyl)-[1,2,4]triazolo[4,3-b][1,2,4]triazole(9)were synthesized through the convenient cyclization reaction from the readily available reactant.Their energetic salts with high nitrogen content were proved to be rare examples of divalent monocyclic/fused cyclic cationic salts according to the single crystal analyses.The structure of intermediate B was also identified and verified by its trivalent cation crystal 17.5H_2O indirectly.Energetic compounds 2-8 and 10-17 were fully characterized by NMR spectroscopy,infrared spectroscopy,differential scanning calorimetry,elemental analysis.These energetic salts exhibit good thermal stability with decomposition temperatures ranged from 182℃to 245℃.The sensitivity of compounds 2,6,10 and 14 is similar or superior to that of RDX while the others were much more insensitive to mechanical stimulate.Furthermore,detonation velocity of 10(8843 m/s)surpass that of RDX(D=8795 m/s).Considering the high gas production volume(≥808 L/kg)of 2,4,10and 12,constant-volume combustion experiments were conduct to evaluate their gas production capacities specifically.These compounds possess much higher maximum gas-production pressures(P_(max):7.88-10.08 MPa)than the commonly used reagent guanidine nitrate(GN:P_(max)=4.20 MPa),which indicate their strong gas production capacity.
文摘The mixed lineage kinase domain-like(MLKL)protein is a key factor in tumor necrosis factor-induced necroptosis.Recent studies on necroptosis execution revealed a commitment role of MLKL in membrane disruption.However,our knowledge of how MLKL functions on membrane remains very limited.Here we demonstrate that MLKL forms cation channels that are permeable preferential y to Mg2+rather than Ca2+in the presence of Na+and K+.Moreover,the N-terminal domain containing six helices(H1-H6)is sufficient to form channels.Using the substituted cysteine accessibility method,we further determine that helix H1,H2,H3,H5 and H6 are transmembrane segments,while H4 is located in the cytoplasm.Finally,MLKL-induced membrane depolarization and cell death exhibit a positive correlation to its channel activity.The Mg2+-preferred permeability and five transmembrane segment topology distinguish MLKL from previously identified Mg2+-permeable channels and thus establish MLKL as a novel class of cation channels.
文摘In this paper,the author defined the interpretation system of geoparks,studied the designing princeples and methods of the interpretation identification system,including the information,content and appearance. Furthermore,the designing of the interpretation identification system designing of the Hanas National Geopark was conducted as an empirical study,
基金supported by State Key Program of Basic Research of China(2013CB910604)National Natural Science Foundation of China(61327014,61175103,61433017 and31571427)the External Cooperation Program of BIC,Chinese Academy of Sciences(1536631KYSB20130003)
文摘OBJECTIVE To investigate how MLKL functions on the membrane and explore its electrophysiological characters and structure.METHODS The full-length human MLKL were expressed in SF21 cells and purified using glutathione-sepharose affinity chromatography.The currents of purified MLKL proteins were recorded in avoltage-clamp mode using a Warner BC-535 bilayer clamp amplifier.The currents were digitized using p CLAMP 10.2 software.HEK293 cells were cultured and transfected with MLKL plasmid.Cell viability was examined using the Cell Titer-Glo Luminescent Cell Viability Assay kit.RESULT MLKL forms cation channels that are permeable preferentially to Mg2+rather than Ca2+in the presence of Na+and K+.Moreover,each MLKL monomer contains five transmembrane helices:H1,H2,H3,H5 and H6 of the N-terminal domain which is sufficient to form channels.Finally,MLKL-induced membrane depolarization and cell death exhibit a positive correlation to its channel activity.
文摘As a strategy for phytopathogen control throughtransgenic way,antimicrobial peptide genes havebeen employed over the last two decades.CEMA is a cationic antimicrobial chimericpeptide,which is produced by fusing eightamino acid residues from the antimicrobialpeptide ceropin A with a modified meltin.Threetransgenic cotton plants were generated
文摘The most remarkable effect in spinel ferrites is the strong dependence of properties on the state of structural disorder and,in particular,on the cation distribution.The structural characterization of a Co-Zn ferrite nanoparticle sample was reported which prepared by wet chemical co-precipitation method.The samples were sintered at three different temperatures viz.650℃,850℃ and 1050℃ for 12 h.The structural details like:lattice constant and distribution of cations in the tetrahedral and octahedral interstitial voids have been deduced through X-ray diffraction (XRD) data analysis.Lattice constant was found to increase with the increase in Zn2+ ions and sintering temperature.Theoretical intensity ratios of (220),(400),(440) planes were considered,as these reflections are sensitive to cations on the A and B sites.Close agreement of the theoretical intensity ratio with the intensity ratio observed from XRD pattern supports the occupancy of Zn2+ ions and Co2+ ions on the octahedral and tetrahedral sites,respectively.
基金supported in part by the National Natural Science Fundation of China(4117131761132008+1 种基金61490693)Aeronautical Science Foundation of China(20132058003)
文摘A novel method is proposed for the supervised classification of multifrequency polarimetric synthetic aperture radar （PolSAR） images. The coherency matrices in P-, L-, and C-bands are mapped onto a 9×9 matrix Ω based on the eigenvalue decomposition of the coherency matrix of each band. A boxcar filter is then performed on the matrix Ω. The filtered data are put into a complex Wishart classifier. Finally, the effectiveness of the proposed method is demonstrated with JPL/AIRSAR multifrequency PolSAR data acquired over the Flevoland area.
基金the National Research Foundation of Korea(20011-0013534,2009-0076930) the National Natural Science Foundation of China(21265022)
文摘The effect of various metal ions on the DNA mediated energy transfer between simultaneously bound drugs was investigated using spectroscopic methods.It was found that addition of divalent metal ions(Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni^(2+))resulted in further decrease of the ethidium fluorescence intensity,while a small increase was observed in the TMPyP emission band,implying that the energy of excited ethidium was transferred to TMPyP.This DNA-mediated quenching efficiency between ethidium and TMPyP was significantly enhanced by the presence of all metal ions.Among the divalent metal ions,alkali earth metal ions and Mn^(2+)displayed higher quenching efficiencies than other transition metal ions.The distances required to permit the energy transfer between the two drugs in DNA were calculated as 68,66,62,48and 38in the presence of100μmol·L^(-1 )of Mg^(2+),Ca^(2+),Mn^(2+),Co^(2+)and Ni ^(2+)ion,respectively.The disturbed binding conformation of TMPyP in DNA by metal ions presumably accounts for the difference.
文摘This contribution is not only intended to provide a fresh review for researches on static electrification, but also contains a sub- stantial amount of new material with a view of closing some gaps in the present state of knowledge of oil streaming electrification. In this paper, the Electrostatic Charging Tendency (ECT) of fresh (unaged) and aged transformer oils were investigated in a spinning disk system. Static electrification measurement at different aging degrees was carried out under laboratory controlled conditions. Changes in static elec- trification were compared to some classical aging indexes (conductivity, dissipation factor, water content, resistivity, etc.). Fast, inexpensive and reliable laboratory testing procedures developed by American Society for Testing Materials (D 6802 and D 6181) were also used to monitor decay products as trace impurities. The obtained results show that static electrification currents increase with temperature, fluid mo- tion velocity, surface properties and the aging byproducts of the oil. The polarity, the amplitude and the time constant of the electrification currents are also affected. The results show that static electrification has relationship with thermal aging and is sensitive to turbidimetric and spectrophotometric measurements.
