采用聚丙烯酰胺凝胶垂直板电泳法对蓝色鳞鲤(Cyprinus carpio blue var)进行同工酶分析。结果表明:在蓝色鳞鲤的眼睛、肌肉、肝脏、心脏、肾脏5种组织中,14种酶(LDH、ADH、GDH、MDH、G-6-PDH、EST、POD、SDH、FDH、SOD、α-AMY、CAT、CO...采用聚丙烯酰胺凝胶垂直板电泳法对蓝色鳞鲤(Cyprinus carpio blue var)进行同工酶分析。结果表明:在蓝色鳞鲤的眼睛、肌肉、肝脏、心脏、肾脏5种组织中,14种酶(LDH、ADH、GDH、MDH、G-6-PDH、EST、POD、SDH、FDH、SOD、α-AMY、CAT、COX、ME)的同工酶谱均存在明显的组织特异性。14种酶共记录出33个基因位点,其中α-Amy-2、Cox-2、Est-1、Ldh-1、Mdh-1、Mdh-2和Sod-1为多态位点。蓝色鳞鲤群体的多态位点百分数为21.21%(P0.99),平均预期杂合度和平均实际杂合度分别为0.1079和0.2121,遗传偏离指数(d)值为正。平均有效等位基因数(Ne)为1.24。实验表明,目前蓝色鳞鲤群体的种质资源状况尚好,表现出明显的杂交优势。展开更多
试验选用初始体重为(44.34±1.58)g的津新鲤1 260尾,随机分为6组,每组3个重复,每个重复70尾鱼。6组饲料分别含有豆油、鸡油、玉米油、棕榈油、菜籽油、棉籽油。饲养8周后探讨饲料中不同脂肪源对津新鲤生长、相关脂代谢酶活性及脂肪...试验选用初始体重为(44.34±1.58)g的津新鲤1 260尾,随机分为6组,每组3个重复,每个重复70尾鱼。6组饲料分别含有豆油、鸡油、玉米油、棕榈油、菜籽油、棉籽油。饲养8周后探讨饲料中不同脂肪源对津新鲤生长、相关脂代谢酶活性及脂肪酸合成酶(FAS)、脂蛋白脂酶(LPL)基因表达的影响。结果表明:玉米油组的相对增重率、特定生长率及蛋白质效率显著高于其它5组(P<0.05),但玉米油组饵料系数则最低。不同脂肪源饲料对肝胰脏及肾脏中LPL、肝脂酶(HL)、总脂酶(GE)、脂肪代谢酶活性影响很大(P<0.05),且玉米油组酶活性均最高;各组肝胰脏及肾脏FAS变化不大(P>0.05)。从基因表达来看,玉米油组更容易诱导和调节肝胰脏和肾脏中FAS及LPL m RNA的表达(P<0.05),再次投喂后6 h,肝胰脏和肾脏中LPL m RNA表达丰度均达到峰值,而肝胰脏中FASm RNA表达丰度达到最低值,肾脏中FAS m RNA表达丰度逐渐下降。总之,玉米油可作为津新鲤优质脂肪源。展开更多
Analysis of fish meat using gas chromatography is described.Flavor is the sensation arising from the interplay of the signals of sensing smell, taste and irritating stimuli from food stuff.For human, flavor and nutrit...Analysis of fish meat using gas chromatography is described.Flavor is the sensation arising from the interplay of the signals of sensing smell, taste and irritating stimuli from food stuff.For human, flavor and nutrition are inseparable.In fish, trace amount of volatile organic compounds (VOCs) are the major compounds to affect consumer’s preference, which are produced during storage and spoilage.In the present study, volatile compounds were extracted by Likens-Nickerson concurrent distillation apparatus from wild and farmed Cirrhina mrigala and Cyprinus carpio. The quantitative and qualitative estimation of volatiles was made by gas chromatography.Wild and farmed fish of different fish sizes were compared for these compounds (appearing in the form of peaks), which were identified from their retention time by comparing with the standards.Fifteen major VOCs were found in these species which included hexadecane, 3-octanol, hexanal, decane, 3-hexene-1-ol, 2-undecanone, 2-heptanone, butanal, 2-nonanone, 1-heptanal, furaldehyde, 3-methyl-1-butanal, trans-3-hexene-1-ol, octanal and decanal.These compounds varied qualitatively and quantitatively in both wild and farmed fish of different fish sizes.展开更多
文摘采用聚丙烯酰胺凝胶垂直板电泳法对蓝色鳞鲤(Cyprinus carpio blue var)进行同工酶分析。结果表明:在蓝色鳞鲤的眼睛、肌肉、肝脏、心脏、肾脏5种组织中,14种酶(LDH、ADH、GDH、MDH、G-6-PDH、EST、POD、SDH、FDH、SOD、α-AMY、CAT、COX、ME)的同工酶谱均存在明显的组织特异性。14种酶共记录出33个基因位点,其中α-Amy-2、Cox-2、Est-1、Ldh-1、Mdh-1、Mdh-2和Sod-1为多态位点。蓝色鳞鲤群体的多态位点百分数为21.21%(P0.99),平均预期杂合度和平均实际杂合度分别为0.1079和0.2121,遗传偏离指数(d)值为正。平均有效等位基因数(Ne)为1.24。实验表明,目前蓝色鳞鲤群体的种质资源状况尚好,表现出明显的杂交优势。
文摘试验选用初始体重为(44.34±1.58)g的津新鲤1 260尾,随机分为6组,每组3个重复,每个重复70尾鱼。6组饲料分别含有豆油、鸡油、玉米油、棕榈油、菜籽油、棉籽油。饲养8周后探讨饲料中不同脂肪源对津新鲤生长、相关脂代谢酶活性及脂肪酸合成酶(FAS)、脂蛋白脂酶(LPL)基因表达的影响。结果表明:玉米油组的相对增重率、特定生长率及蛋白质效率显著高于其它5组(P<0.05),但玉米油组饵料系数则最低。不同脂肪源饲料对肝胰脏及肾脏中LPL、肝脂酶(HL)、总脂酶(GE)、脂肪代谢酶活性影响很大(P<0.05),且玉米油组酶活性均最高;各组肝胰脏及肾脏FAS变化不大(P>0.05)。从基因表达来看,玉米油组更容易诱导和调节肝胰脏和肾脏中FAS及LPL m RNA的表达(P<0.05),再次投喂后6 h,肝胰脏和肾脏中LPL m RNA表达丰度均达到峰值,而肝胰脏中FASm RNA表达丰度达到最低值,肾脏中FAS m RNA表达丰度逐渐下降。总之,玉米油可作为津新鲤优质脂肪源。
文摘Analysis of fish meat using gas chromatography is described.Flavor is the sensation arising from the interplay of the signals of sensing smell, taste and irritating stimuli from food stuff.For human, flavor and nutrition are inseparable.In fish, trace amount of volatile organic compounds (VOCs) are the major compounds to affect consumer’s preference, which are produced during storage and spoilage.In the present study, volatile compounds were extracted by Likens-Nickerson concurrent distillation apparatus from wild and farmed Cirrhina mrigala and Cyprinus carpio. The quantitative and qualitative estimation of volatiles was made by gas chromatography.Wild and farmed fish of different fish sizes were compared for these compounds (appearing in the form of peaks), which were identified from their retention time by comparing with the standards.Fifteen major VOCs were found in these species which included hexadecane, 3-octanol, hexanal, decane, 3-hexene-1-ol, 2-undecanone, 2-heptanone, butanal, 2-nonanone, 1-heptanal, furaldehyde, 3-methyl-1-butanal, trans-3-hexene-1-ol, octanal and decanal.These compounds varied qualitatively and quantitatively in both wild and farmed fish of different fish sizes.
