OBJECTIVE To investigate the inhibitory effects of Spatholobus Suberectus Column Extract(SSCE)on estrogen receptor positive(ER+)breast cancer cel MCF-7and its possible molecular mechanism.METHODS MCF-7cells were cultu...OBJECTIVE To investigate the inhibitory effects of Spatholobus Suberectus Column Extract(SSCE)on estrogen receptor positive(ER+)breast cancer cel MCF-7and its possible molecular mechanism.METHODS MCF-7cells were cultured without estrogen and with 17-β-estrogen(10-8mol·L-1),respectively,then treated with SSCE(0,40,80,160,320μg·m L-1).MTT assay was employed to evaluate cell viability.Flow cytometry assays were performed to underlying apoptosis and detecting cel cycle of MCF-7 cells treated with SSCE(0,80,160,320μg·mL-1).Wound healing assays was conducted to detect the migration ability.Dual luciferase reporter system was used to detect the activity of p-ERα,p-ERβpresented in intra-nuclear estrogen response element(ERE).Western blotting assay was employed to identify the expression of protein such as Bax,Bcl-2,p-ERα,p-ERβ,ERK1/2,p-ERK1/2,AKT,p-AKT,m TOR,p-m TOR,PI3K,p-PI3K.RESULTS It showed that SSCE(80,160and 320μg·mL-1)significantly decreased the viability of MCF-7.SSCE also triggered apoptosis,arrested cell cycle at G0/G1phase,inhibited migration.Dual luciferase reporter system showed that SSCE suppressed intra-nuclear p-ER activity,Western blotting analysis confirmed that SSCE did repress the expression of phosphorylated-ER alpha(p-ERα),ERK1/2,p-ERK1/2,AKT,p-AKT,pmT OR,PI3K,p-PI3K,which indicate that SSCE suppress MAPK PI3K/AKT signal pathway.CONCLUSION Our result showed that SSCE cause ER+MCF-7 cells apoptosis,G0/G1phase arresting,migration decreasing,via hypo-active of ER,suppress MAPK PI3K/AKT pathway.展开更多
患者女,48岁,外院体检发现左乳肿物2年、无明显增大。查体:分别于右乳内上象限及左乳外上象限触及2.5cm×2cm、2.0cm×2.0 cm肿物,质硬,活动度可。乳腺超声:双乳多发低回声结节,左乳外上象限不均质回声区,乳腺影像报告和数据系...患者女,48岁,外院体检发现左乳肿物2年、无明显增大。查体:分别于右乳内上象限及左乳外上象限触及2.5cm×2cm、2.0cm×2.0 cm肿物,质硬,活动度可。乳腺超声:双乳多发低回声结节,左乳外上象限不均质回声区,乳腺影像报告和数据系统(breast imaging reporting and data system,BIRADS)3级。展开更多
乳腺分泌性癌(secretory carcinoma of the breast, SCB)是一种罕见的乳腺恶性肿瘤,约占所有类型乳腺癌的0.02%,ETV6-NTRK3融合基因是其特征性基因改变。尽管多数情况下具有三阴表型,SCB通常预后良好,罕见复发、转移,但相关的分子基因...乳腺分泌性癌(secretory carcinoma of the breast, SCB)是一种罕见的乳腺恶性肿瘤,约占所有类型乳腺癌的0.02%,ETV6-NTRK3融合基因是其特征性基因改变。尽管多数情况下具有三阴表型,SCB通常预后良好,罕见复发、转移,但相关的分子基因组学改变仍未明确。本文报道了1例TERT启动子突变的复发SCB患者的临床资料、组织学特征、免疫表型及分子基因组学改变,并复习相关文献,从而加深对SCB的认识。展开更多
基金The project supported by Beijing Natural Science Foundation(7122083)Beijing Science and Technology Projec(tD161100005116005)
文摘OBJECTIVE To investigate the inhibitory effects of Spatholobus Suberectus Column Extract(SSCE)on estrogen receptor positive(ER+)breast cancer cel MCF-7and its possible molecular mechanism.METHODS MCF-7cells were cultured without estrogen and with 17-β-estrogen(10-8mol·L-1),respectively,then treated with SSCE(0,40,80,160,320μg·m L-1).MTT assay was employed to evaluate cell viability.Flow cytometry assays were performed to underlying apoptosis and detecting cel cycle of MCF-7 cells treated with SSCE(0,80,160,320μg·mL-1).Wound healing assays was conducted to detect the migration ability.Dual luciferase reporter system was used to detect the activity of p-ERα,p-ERβpresented in intra-nuclear estrogen response element(ERE).Western blotting assay was employed to identify the expression of protein such as Bax,Bcl-2,p-ERα,p-ERβ,ERK1/2,p-ERK1/2,AKT,p-AKT,m TOR,p-m TOR,PI3K,p-PI3K.RESULTS It showed that SSCE(80,160and 320μg·mL-1)significantly decreased the viability of MCF-7.SSCE also triggered apoptosis,arrested cell cycle at G0/G1phase,inhibited migration.Dual luciferase reporter system showed that SSCE suppressed intra-nuclear p-ER activity,Western blotting analysis confirmed that SSCE did repress the expression of phosphorylated-ER alpha(p-ERα),ERK1/2,p-ERK1/2,AKT,p-AKT,pmT OR,PI3K,p-PI3K,which indicate that SSCE suppress MAPK PI3K/AKT signal pathway.CONCLUSION Our result showed that SSCE cause ER+MCF-7 cells apoptosis,G0/G1phase arresting,migration decreasing,via hypo-active of ER,suppress MAPK PI3K/AKT pathway.
文摘患者女,48岁,外院体检发现左乳肿物2年、无明显增大。查体:分别于右乳内上象限及左乳外上象限触及2.5cm×2cm、2.0cm×2.0 cm肿物,质硬,活动度可。乳腺超声:双乳多发低回声结节,左乳外上象限不均质回声区,乳腺影像报告和数据系统(breast imaging reporting and data system,BIRADS)3级。
文摘乳腺分泌性癌(secretory carcinoma of the breast, SCB)是一种罕见的乳腺恶性肿瘤,约占所有类型乳腺癌的0.02%,ETV6-NTRK3融合基因是其特征性基因改变。尽管多数情况下具有三阴表型,SCB通常预后良好,罕见复发、转移,但相关的分子基因组学改变仍未明确。本文报道了1例TERT启动子突变的复发SCB患者的临床资料、组织学特征、免疫表型及分子基因组学改变,并复习相关文献,从而加深对SCB的认识。
