The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary ...The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary to make clear the molecular genetic characteristics,edible safety,planting,processing,and other aspects of the safety evaluation of GM crops.The safety problems existing in the cultivation of GM crops,safety evaluation and detection of GM crops were introduced in this paper,which provided the basis for safety evaluation and effective supervision of GM crops and their products.Commercial cultivation and reasonable supervision based on safety evaluation have far-reaching significance for ensuring consumer safety,enhancing the credibility of the national political system and enhancing citizens'confidence in the safety of GM crop products for consumption.展开更多
[Aim]In this study,the potential side effects of the sublethal doses of imidacloprid on the midgut bacteria of the worker bees of Apis mellifera ligustica was investigated under laboratory conditions so as to provide ...[Aim]In this study,the potential side effects of the sublethal doses of imidacloprid on the midgut bacteria of the worker bees of Apis mellifera ligustica was investigated under laboratory conditions so as to provide a theoretical basis for the biosafety assessment of pesticides.[Methods]Newly emerged bees were fed on syrups with different sublethal doses of imidacloprid(0.005 mg/L,0.015 mg/L and 0.045 mg/L)and pure sugar syrup with 0.3%acetone,and samples were collected after 3,6,9,12 and 15 d,respectively.The population structure of bacteria in the midgut of honeybees in the four treatment groups was analyzed using next-generation sequencing after the genomic DNA was gained from intestinal bacteria.[Results]The results of sequence analysis showed that the midgut bacteria in A.mellifera ligustica mainly belong to Proteobacteria and Firmicutes.The composition of dominant midgut bacteria and the richness estimators in the same period were analyzed for statistical significance among the four treatments by performing a T-test,and no significant differences were observed(P〉0.05).Conclusion]The results suggest that the sublethal doses of imidacloprid does not induce significant hanges in the composition of the honeybee gut bacterial community under laboratory conditions.展开更多
基金Supported by the China Postdoctoral Science Foundation(2023M730312)the Science and Technology Plan Projects of the State Administration for Market Regulation(2022MK002)the National Key Research and Development Program(2022YFF0606105)。
文摘The commercial cultivation of genetically modified(GM)crops has eased the global food crisis and brought considerable economic and social benefits to countries.Because of the potential safety problems,it is necessary to make clear the molecular genetic characteristics,edible safety,planting,processing,and other aspects of the safety evaluation of GM crops.The safety problems existing in the cultivation of GM crops,safety evaluation and detection of GM crops were introduced in this paper,which provided the basis for safety evaluation and effective supervision of GM crops and their products.Commercial cultivation and reasonable supervision based on safety evaluation have far-reaching significance for ensuring consumer safety,enhancing the credibility of the national political system and enhancing citizens'confidence in the safety of GM crop products for consumption.
文摘[Aim]In this study,the potential side effects of the sublethal doses of imidacloprid on the midgut bacteria of the worker bees of Apis mellifera ligustica was investigated under laboratory conditions so as to provide a theoretical basis for the biosafety assessment of pesticides.[Methods]Newly emerged bees were fed on syrups with different sublethal doses of imidacloprid(0.005 mg/L,0.015 mg/L and 0.045 mg/L)and pure sugar syrup with 0.3%acetone,and samples were collected after 3,6,9,12 and 15 d,respectively.The population structure of bacteria in the midgut of honeybees in the four treatment groups was analyzed using next-generation sequencing after the genomic DNA was gained from intestinal bacteria.[Results]The results of sequence analysis showed that the midgut bacteria in A.mellifera ligustica mainly belong to Proteobacteria and Firmicutes.The composition of dominant midgut bacteria and the richness estimators in the same period were analyzed for statistical significance among the four treatments by performing a T-test,and no significant differences were observed(P〉0.05).Conclusion]The results suggest that the sublethal doses of imidacloprid does not induce significant hanges in the composition of the honeybee gut bacterial community under laboratory conditions.
