Rape bee pollen has attracted increasing interests for its excellent protective effect against chemicalinduced liver injury owing to its abundant polyphenols.This study aims to analyze the types and contents of phenol...Rape bee pollen has attracted increasing interests for its excellent protective effect against chemicalinduced liver injury owing to its abundant polyphenols.This study aims to analyze the types and contents of phenolamides(seldom concerned)in rape bee pollen and their protective mechanism on alcoholic liver disease(ALD).Different from the previous finding that flavonoids are dominant polyphenols in bee pollen polyphenolic extract,our results demonstrated that there are only three flavonoids but 24 phenolamides in the as-prepared rape bee pollen phenolic extract(PPE).In addition,PPE was found to significantly improve the viability(from 54.9%to 84.1%,89.2%,and 94.0%)of alcohol-induced AML12 cells and alleviate alcoholinduced cell apoptosis(from 28.5%to 22.89%,22.0%,and 17.4%).To dissect the underlying mechanism for the protective effect of PPE against ALD,the molecular pathway was identified by RNA-Seq analysis.Transcriptome data revealed that PPE may protect against ALD by decreasing inflammation,cholesterol,and fatty acid synthesis(P<0.05).The National Institute on Alcohol Abuse and Alcoholism(NIAAA)model was used to further evaluate the hepatoprotective effect of PPE in vivo,and the results validated that PPE could alleviate liver injury and hepatic steatosis(from 22.7%to 11.5%and 10.9%)induced by alcohol.As the dominant polyphenols in PPE,phenolamides can be a class of valuable polyphenolic compounds in bee pollen with the potential to alleviate ALD.展开更多
The nutrient digestion,absorption and biological activity of bee pollen may be limited due to the complex pollen wall.Here,the effect of superfine grinding technology on the release of nutrients from bee pollen were i...The nutrient digestion,absorption and biological activity of bee pollen may be limited due to the complex pollen wall.Here,the effect of superfine grinding technology on the release of nutrients from bee pollen were investigated,and their antioxidant activities and in vitro digestion were explored in this study.Results showed that the content of nutrients in bee pollen increased after wall disruption.Among them,fat content increased by 22.55%-8.31%,protein content increased by 0.54%-4.91%,starch content increased by 36.31%-48.64%,soluble sugar content increased by 20.57%-29.67%,total phenolic acid content increased by 11.73%-86.98%and total flavonoids content increased by 14.29%-24.79%.At the same time,the antioxidant activity increased by 14.84%-46.00%.Furthermore,the active components such as phenolic compounds in the wall-disruption bee pollen were more readily to be released during the in vitro digestion,and easier to be absorbed because of their higher bioaccessibility.Antioxidant activities during in vitro digestion were also improved in walldisruption bee pollen.These findings provide evidence that bee pollen wall disruption was suggested,thus,it is more conducive to exerting the value of bee pollen in functional foods.展开更多
The significant demand for high quality food has motivated us to adopt appropriate processing methods to improve the food nutritional quality and flavors.In this study,the effects of five drying methods,namely,pulsed ...The significant demand for high quality food has motivated us to adopt appropriate processing methods to improve the food nutritional quality and flavors.In this study,the effects of five drying methods,namely,pulsed vacuum drying(PVD),freeze drying(FD),infrared drying(IRD),hot-air drying(HAD)and sun drying(SD)on free amino acids(FAAs),α-dicarbonyl compounds(α-DCs)and volatile compounds(VOCs)in rape bee pollen(RBP)were determined.The results showed that FD significantly released the essential amino acids(EAAs)compared with fresh samples while SD caused the highest loss.Glucosone was the dominantα-DCs in RBP and the highest loss was observed after PVD.Aldehydes were the dominant volatiles of RBP and SD samples contained more new volatile substances(especially aldehydes)than the other four drying methods.Comprehensively,FD and PVD would be potential methods to effectively reduce the quality deterioration of RBP in the drying process.展开更多
Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lac...Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.展开更多
Bee pollen has potential in preventing metabolic syndrome(MetS).The present study aimed to investigate the effect of yeast-fermented wall-broken bee pollen(YB)intervention on ICR mice with MetS induced with a high-fat...Bee pollen has potential in preventing metabolic syndrome(MetS).The present study aimed to investigate the effect of yeast-fermented wall-broken bee pollen(YB)intervention on ICR mice with MetS induced with a high-fat(HF)diet.After YB intervention in mice for 16 weeks,the effect on alleviating MetS was evaluated based on MetS serum parameters,hepatic oxidant status markers and gut microbial populations.The results of animal experiment showed that YB intervention attenuated MetS.Based on multivariate statistical analysis results,YB treatment signifi cantly increased glutathione S-transferase(GST)and catalase(CAT)activities and decreased the malondialdehyde(MDA)level in the liver.Further investigation showed that YB restored the Nrf-2-Keap-1 pathway to alleviate oxidative stress.Additionally,gut microbial community analysis revealed that YB restored the increase in the Firmicutes to Bacteroidetes(F/B)ratio(6.94 for the HF group and 3.74 for HF+YB group)and improved Lactobacillus and Lactococcus abundance induced by the HF diet.Overall,YB improved function and prevented MetS by modulating the gut microbiota and alleviating oxidative stress.展开更多
基金supported by Key Research and Development Program of Hubei Province(2022BBA0072)Applied Fundamental Frontier Project of Wuhan Science and Technology Bureau(2020020601012271)。
文摘Rape bee pollen has attracted increasing interests for its excellent protective effect against chemicalinduced liver injury owing to its abundant polyphenols.This study aims to analyze the types and contents of phenolamides(seldom concerned)in rape bee pollen and their protective mechanism on alcoholic liver disease(ALD).Different from the previous finding that flavonoids are dominant polyphenols in bee pollen polyphenolic extract,our results demonstrated that there are only three flavonoids but 24 phenolamides in the as-prepared rape bee pollen phenolic extract(PPE).In addition,PPE was found to significantly improve the viability(from 54.9%to 84.1%,89.2%,and 94.0%)of alcohol-induced AML12 cells and alleviate alcoholinduced cell apoptosis(from 28.5%to 22.89%,22.0%,and 17.4%).To dissect the underlying mechanism for the protective effect of PPE against ALD,the molecular pathway was identified by RNA-Seq analysis.Transcriptome data revealed that PPE may protect against ALD by decreasing inflammation,cholesterol,and fatty acid synthesis(P<0.05).The National Institute on Alcohol Abuse and Alcoholism(NIAAA)model was used to further evaluate the hepatoprotective effect of PPE in vivo,and the results validated that PPE could alleviate liver injury and hepatic steatosis(from 22.7%to 11.5%and 10.9%)induced by alcohol.As the dominant polyphenols in PPE,phenolamides can be a class of valuable polyphenolic compounds in bee pollen with the potential to alleviate ALD.
基金the Program of State Key Laboratory of Food Science and Technology,Nanchang University (SKLF-ZZB-202119)。
文摘The nutrient digestion,absorption and biological activity of bee pollen may be limited due to the complex pollen wall.Here,the effect of superfine grinding technology on the release of nutrients from bee pollen were investigated,and their antioxidant activities and in vitro digestion were explored in this study.Results showed that the content of nutrients in bee pollen increased after wall disruption.Among them,fat content increased by 22.55%-8.31%,protein content increased by 0.54%-4.91%,starch content increased by 36.31%-48.64%,soluble sugar content increased by 20.57%-29.67%,total phenolic acid content increased by 11.73%-86.98%and total flavonoids content increased by 14.29%-24.79%.At the same time,the antioxidant activity increased by 14.84%-46.00%.Furthermore,the active components such as phenolic compounds in the wall-disruption bee pollen were more readily to be released during the in vitro digestion,and easier to be absorbed because of their higher bioaccessibility.Antioxidant activities during in vitro digestion were also improved in walldisruption bee pollen.These findings provide evidence that bee pollen wall disruption was suggested,thus,it is more conducive to exerting the value of bee pollen in functional foods.
基金supported by National Natural Science Foundation of China(Nos.31871861 and 31501548)The Apicultural Industry Technology System(NCYTI-43-KXJ17)The Science and Technology Innovation Project of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-2015-IAR)。
文摘The significant demand for high quality food has motivated us to adopt appropriate processing methods to improve the food nutritional quality and flavors.In this study,the effects of five drying methods,namely,pulsed vacuum drying(PVD),freeze drying(FD),infrared drying(IRD),hot-air drying(HAD)and sun drying(SD)on free amino acids(FAAs),α-dicarbonyl compounds(α-DCs)and volatile compounds(VOCs)in rape bee pollen(RBP)were determined.The results showed that FD significantly released the essential amino acids(EAAs)compared with fresh samples while SD caused the highest loss.Glucosone was the dominantα-DCs in RBP and the highest loss was observed after PVD.Aldehydes were the dominant volatiles of RBP and SD samples contained more new volatile substances(especially aldehydes)than the other four drying methods.Comprehensively,FD and PVD would be potential methods to effectively reduce the quality deterioration of RBP in the drying process.
基金supported by the National Natural Science Foundation of China(32102605)the Agricultural Science and Technology Innovation Program under Grant(CAAS-ASTIP-2020-IAR)。
文摘Food allergy as a global health problem threatens food industry.Bee pollen(BP)is a typical food with allergenic potentials,although it performs various nutritional/pharmacological functions to humans.In this study,lactic acid bacteria(LAB)were used to ferment Brassica napus BP for alleviating its allergenicity.Four novel allergens(glutaredoxin,oleosin-B2,catalase and lipase)were identified with significant decreases in LAB-fermented BP(FBP)than natural BP by proteomics.Meanwhile,metabolomics analysis showed significant increases of 28 characteristic oligopeptides and amino acids in FBP versus BP,indicating the degradation of LAB on allergens.Moreover,FBP showed alleviatory effects in BALB/c mice,which relieved pathological symptoms and lowered production of allergic mediators.Microbial high-throughput sequencing analysis showed that FBP could regulate gut microbiota and metabolism to strengthen immunity,which were closely correlated with the alleviation of allergic reactivity.These findings could contribute to the development and utilization of hypoallergenic BP products.
基金This study was supported by the National Natural Science Foundation of China(No.31972628 and 31472155)a special fund(NYCYTX-43)the fund of the Beijing Laboratory for Food Quality and Safety,Beijing Technology and Business University.
文摘Bee pollen has potential in preventing metabolic syndrome(MetS).The present study aimed to investigate the effect of yeast-fermented wall-broken bee pollen(YB)intervention on ICR mice with MetS induced with a high-fat(HF)diet.After YB intervention in mice for 16 weeks,the effect on alleviating MetS was evaluated based on MetS serum parameters,hepatic oxidant status markers and gut microbial populations.The results of animal experiment showed that YB intervention attenuated MetS.Based on multivariate statistical analysis results,YB treatment signifi cantly increased glutathione S-transferase(GST)and catalase(CAT)activities and decreased the malondialdehyde(MDA)level in the liver.Further investigation showed that YB restored the Nrf-2-Keap-1 pathway to alleviate oxidative stress.Additionally,gut microbial community analysis revealed that YB restored the increase in the Firmicutes to Bacteroidetes(F/B)ratio(6.94 for the HF group and 3.74 for HF+YB group)and improved Lactobacillus and Lactococcus abundance induced by the HF diet.Overall,YB improved function and prevented MetS by modulating the gut microbiota and alleviating oxidative stress.
