Coffee is commonly consumed beverage in the world and it has been suggested to have beneficial effect.Chlorogenic acids(CGAs)are main ingredient of coffee beans which has been extensively used in nutraceuticals and me...Coffee is commonly consumed beverage in the world and it has been suggested to have beneficial effect.Chlorogenic acids(CGAs)are main ingredient of coffee beans which has been extensively used in nutraceuticals and medicine.Recently,various therapeutic effects of chlorogenic acids have been investigated.However,there are limited studies to investigate its anticancer properties.In the present study,we have used chlorogenic acid complex(CGA7)a decaffeinated water soluble green coffee bean extract to evaluate its cytotoxic effect on human and mouse cancer cell lines by using different approaches.From our results we found CGA7 treatment induces cell death in a dose and time dependent manner in different cancer cell lines.Further,CGA7 induced apoptosis was characterized by DNA fragmentation,PARP-1 cleavage,caspase-9 activation,and down regulation of Bcl-2,an anti-apoptotic protein and up regulation of pro-apoptotic protein BAX.Overall findings indicated that CGA7 complex a potent anticancer molecule found in green coffee beans could be a safe bioactive ingredient for prevention of cancer.展开更多
Clenbuterol and some kinds of antibiotic are banned strictly using as veterinary drug by Europe Union,America and Chinese Agriculture Ministry.Electronic irradiation can change difficultly degraded pollutants into eas...Clenbuterol and some kinds of antibiotic are banned strictly using as veterinary drug by Europe Union,America and Chinese Agriculture Ministry.Electronic irradiation can change difficultly degraded pollutants into easily degraded pollutants.Clenbuterol,SC and AOZ can be degraded more than 95% when the irradiation dosage was greater than 6kGy.These results indicated such irradiation method is applicable.展开更多
文摘Coffee is commonly consumed beverage in the world and it has been suggested to have beneficial effect.Chlorogenic acids(CGAs)are main ingredient of coffee beans which has been extensively used in nutraceuticals and medicine.Recently,various therapeutic effects of chlorogenic acids have been investigated.However,there are limited studies to investigate its anticancer properties.In the present study,we have used chlorogenic acid complex(CGA7)a decaffeinated water soluble green coffee bean extract to evaluate its cytotoxic effect on human and mouse cancer cell lines by using different approaches.From our results we found CGA7 treatment induces cell death in a dose and time dependent manner in different cancer cell lines.Further,CGA7 induced apoptosis was characterized by DNA fragmentation,PARP-1 cleavage,caspase-9 activation,and down regulation of Bcl-2,an anti-apoptotic protein and up regulation of pro-apoptotic protein BAX.Overall findings indicated that CGA7 complex a potent anticancer molecule found in green coffee beans could be a safe bioactive ingredient for prevention of cancer.
文摘Clenbuterol and some kinds of antibiotic are banned strictly using as veterinary drug by Europe Union,America and Chinese Agriculture Ministry.Electronic irradiation can change difficultly degraded pollutants into easily degraded pollutants.Clenbuterol,SC and AOZ can be degraded more than 95% when the irradiation dosage was greater than 6kGy.These results indicated such irradiation method is applicable.
