【目的】应用线粒体DNA条形码技术对尤犀金龟属(Eupatorus Burmeister,1847)昆虫物种界定进行探索,以解决该属物种形态鉴定困难的问题。【方法】基于尤犀金龟属物种线粒体cox1和cox2基因序列数据集,使用Automatic Barcode Gap Discovery...【目的】应用线粒体DNA条形码技术对尤犀金龟属(Eupatorus Burmeister,1847)昆虫物种界定进行探索,以解决该属物种形态鉴定困难的问题。【方法】基于尤犀金龟属物种线粒体cox1和cox2基因序列数据集,使用Automatic Barcode Gap Discovery(ABGD)和Bayesian Poisson Tree Processes(bPTP)对3个形态种进行分子物种界定,并与形态学鉴定结果进行比较。【结果】使用ABGD方法时,cox1数据集的界定结果与形态学鉴定结果一致,cox2数据集的界定结果与形态学鉴定结果存在差异;使用bPTP方法时,2种数据集的界定结果均远高于形态学鉴定结果,且均存在不同程度的过度划分。【结论】cox1是更适合用于鉴定尤犀金龟属昆虫的DNA条形码,使用ABGD方法时,其数据集界定结果与形态学鉴定结果一致。利用分子界定与形态特征鉴定相结合,可极大地提高鉴定效率和准确性。展开更多
The East China Sea(ECS)off the Coast of Zhoushan Archipelago,Zhejiang(ECS-CZA)is home to abundant fishery resources and an important spawning,feeding,and nursing ground for a variety of fish species.Due to long-term o...The East China Sea(ECS)off the Coast of Zhoushan Archipelago,Zhejiang(ECS-CZA)is home to abundant fishery resources and an important spawning,feeding,and nursing ground for a variety of fish species.Due to long-term overfishing,the ichthyoplankton structure has been dramatically altered.Understanding the species composition and distribution of fish eggs and larvae is one of the most essential tasks to accurately regulate fishery resources and formulate effective management policies;however,little is known about the ichthyoplankton in this region.In this study,an integrated strategy of morphology identification(MI)and mitochondrial COI DNA barcoding was used to identify species of fish eggs and larvae collected from the ECSCZA.MI revealed 15 fish egg species belonging to 12 families and 12 fish larva species belonging to 12 families;in contrast,DNA barcoding altogether identified 30 species,including 18 fish egg species and 13 fish larva species.One species was shared between the egg and larva samples.Our study offers useful tools and critical scientific information for further understanding the diversity,distribution,and conservation management of various ichthyoplankton species in the marine environment.展开更多
The application of molecular tools for the identification of strains,populations and species has become a common practice in a variety of applied and basic investigations in many parts of the world.However,standardiza...The application of molecular tools for the identification of strains,populations and species has become a common practice in a variety of applied and basic investigations in many parts of the world.However,standardization of such applications varies widely among organisms and scientific fields.In this mini-review,the author provides a brief introduction to one of the most prominent effort for species identification-the international barcode of life(iBOL) project,discusses the features of fungal diversity including the proposed fungal barcode DNA fragment-the intergenic spacer regions(ITS) of the nuclear ribosomal RNA gene clusters,and illustrates the potential promises and problems of using ITS for barcoding and for analyzing the phylogeographic pattern of the wild gourmet mushroom Tricholoma matsutake species complex.The analyses show that identification to species level is often insufficient for practical applications and that sequences from multiple genes in combination with critical morphological and physiological evaluations are needed to identify strains,populations and species.展开更多
文摘【目的】应用线粒体DNA条形码技术对尤犀金龟属(Eupatorus Burmeister,1847)昆虫物种界定进行探索,以解决该属物种形态鉴定困难的问题。【方法】基于尤犀金龟属物种线粒体cox1和cox2基因序列数据集,使用Automatic Barcode Gap Discovery(ABGD)和Bayesian Poisson Tree Processes(bPTP)对3个形态种进行分子物种界定,并与形态学鉴定结果进行比较。【结果】使用ABGD方法时,cox1数据集的界定结果与形态学鉴定结果一致,cox2数据集的界定结果与形态学鉴定结果存在差异;使用bPTP方法时,2种数据集的界定结果均远高于形态学鉴定结果,且均存在不同程度的过度划分。【结论】cox1是更适合用于鉴定尤犀金龟属昆虫的DNA条形码,使用ABGD方法时,其数据集界定结果与形态学鉴定结果一致。利用分子界定与形态特征鉴定相结合,可极大地提高鉴定效率和准确性。
文摘The East China Sea(ECS)off the Coast of Zhoushan Archipelago,Zhejiang(ECS-CZA)is home to abundant fishery resources and an important spawning,feeding,and nursing ground for a variety of fish species.Due to long-term overfishing,the ichthyoplankton structure has been dramatically altered.Understanding the species composition and distribution of fish eggs and larvae is one of the most essential tasks to accurately regulate fishery resources and formulate effective management policies;however,little is known about the ichthyoplankton in this region.In this study,an integrated strategy of morphology identification(MI)and mitochondrial COI DNA barcoding was used to identify species of fish eggs and larvae collected from the ECSCZA.MI revealed 15 fish egg species belonging to 12 families and 12 fish larva species belonging to 12 families;in contrast,DNA barcoding altogether identified 30 species,including 18 fish egg species and 13 fish larva species.One species was shared between the egg and larva samples.Our study offers useful tools and critical scientific information for further understanding the diversity,distribution,and conservation management of various ichthyoplankton species in the marine environment.
文摘The application of molecular tools for the identification of strains,populations and species has become a common practice in a variety of applied and basic investigations in many parts of the world.However,standardization of such applications varies widely among organisms and scientific fields.In this mini-review,the author provides a brief introduction to one of the most prominent effort for species identification-the international barcode of life(iBOL) project,discusses the features of fungal diversity including the proposed fungal barcode DNA fragment-the intergenic spacer regions(ITS) of the nuclear ribosomal RNA gene clusters,and illustrates the potential promises and problems of using ITS for barcoding and for analyzing the phylogeographic pattern of the wild gourmet mushroom Tricholoma matsutake species complex.The analyses show that identification to species level is often insufficient for practical applications and that sequences from multiple genes in combination with critical morphological and physiological evaluations are needed to identify strains,populations and species.
