Axillary buds from 3-yr.-old seedlings of Camptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the e...Axillary buds from 3-yr.-old seedlings of Camptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the effects of different basal media, different concen- trations of growth regulators (BA or TDZ), sucrose, agar and pH value on shoot regeneration from axillary bud. The results showed that B5 and WPM media were the optimal basal media and the optimal phyotohormone was BA of 1.0 mg/L or TDZ of 0.1mg/L; The concentrations of sucrose of 30g/L and agar of 6g/L were most suitable for the shoot regeneration; pH value from 5.8 to 6.6 were broadly effective, but the best at pH 5.8.展开更多
BACKGROUND:Fever in patients can provide an important clue to the etiology of a patient's symptoms.Non-invasive temperature sites(oral,axillary,temporal) may be insensitive due to a variety of factors.This has not...BACKGROUND:Fever in patients can provide an important clue to the etiology of a patient's symptoms.Non-invasive temperature sites(oral,axillary,temporal) may be insensitive due to a variety of factors.This has not been well studied in adult emergency department patients.To determine whether emergency department triage temperatures detected fever adequately when compared to a rectal temperature.METHODS:A retrospective chart review was made of 27 130 adult patients in a high volume,urban emergency department over an eight-year period who received first a non-rectal triage temperature and then a subsequent rectal temperature.RESULTS:The mean difference in temperatures between the initial temperature and the rectal temperature was 1.3 °F(P<0.001),with 25.9%of the patients having higher rectal temperatures >2°F,and 5.0%having higher rectal temperatures >4 °F.The mean difference among the patients who received oral,axillary,and temporal temperatures was 1.2 °F(PO.001),1.8 °F(PO.001),and 1.2 °F(P<0.001) respectively.About 18.1%of the patients were initially afebrile and found to be febrile by rectal temperature,with an average difference of 2.5 °F(P<0.001).These patients had a higher rate of admission(61.4%,P<0.005),and were more likely to be admitted to the hospital for a higher level of care,such as an intensive care unit,when compared with the full cohort(12.5%vs.5.8%,P<0.005).CONCLUSIONS:There are significant differences between rectal temperatures and noninvasive triage temperatures in this emergency department cohort.In almost one in five patients,fever was missed by triage temperature.展开更多
Thamnocalamus falconeri, Hook.f. ex Munro.,an important bamboo species belonging to the family Poaceae, locally known as Ringal, occurs in the hills of Uttarakhand, India. This species has been traditionally exploited...Thamnocalamus falconeri, Hook.f. ex Munro.,an important bamboo species belonging to the family Poaceae, locally known as Ringal, occurs in the hills of Uttarakhand, India. This species has been traditionally exploited by local communities to support their livelihoods.Increasing needs of the hill villages impose unsustainable pressure on natural stands of Ringal in the Uttarakhand hills and forests have been degraded. The long history of excessive cutting of Ringal from natural forests and the lack of replanting threaten villager livelihoods. Replanting is required to conserve the species. We propose a protocol for generation of planting material through axillary bud proliferation for multiplication and conservation of this species. We collected offsets/rhizomes from a natural stand of T. falconeri in the Chopta Mandal areas（Chamoli district, India）. These were planted at sites of varied elevation and fresh single nodal segments were collected from them as explants. Different sterilization treatments were assessed to combat contamination. Among these, treatment of 0.1 %Hg Cl2 followed by 5 % Na OCl, proved best. Among two cytokinin treatments, viz. BAP and Kinetin, singly or in combination, BAP alone（5 mg L-1） proved superior and resulted in 100 % bud break. BAP-supplemented MS media yielded maximum vigorous shoot formation（90 %）and maximum number of shoots（8.9）. Subculturing of shoots on the same medium with similar BAP treatment（5 mg L-1BAP） enabled continuous production of healthy shoots at similar frequency. Maximum rooting（100 %）was recorded on half-strength MS medium supplemented with 5 mg L-1IBA. Micropropagated plants were hardened and acclimatized in soil mixture（2：1：1） and then transplanted to field sites（Magra, Uttarakhand, 1,834 m）.Eight to ten months after field transplantation we recorded100 % survival of transplanted material. This micropropagation protocol could be used successfully for raising a stock of genetically homogenous plant material in bulk for field plantations and for conservation of the species.展开更多
基金教育部重点项目，Application Fund of Agricultural Research Production
文摘Axillary buds from 3-yr.-old seedlings of Camptotheca acuminata in the greenhouse were cultured on the different basal media with different concentrations of growth regulators for shoot regeneration for studying the effects of different basal media, different concen- trations of growth regulators (BA or TDZ), sucrose, agar and pH value on shoot regeneration from axillary bud. The results showed that B5 and WPM media were the optimal basal media and the optimal phyotohormone was BA of 1.0 mg/L or TDZ of 0.1mg/L; The concentrations of sucrose of 30g/L and agar of 6g/L were most suitable for the shoot regeneration; pH value from 5.8 to 6.6 were broadly effective, but the best at pH 5.8.
文摘BACKGROUND:Fever in patients can provide an important clue to the etiology of a patient's symptoms.Non-invasive temperature sites(oral,axillary,temporal) may be insensitive due to a variety of factors.This has not been well studied in adult emergency department patients.To determine whether emergency department triage temperatures detected fever adequately when compared to a rectal temperature.METHODS:A retrospective chart review was made of 27 130 adult patients in a high volume,urban emergency department over an eight-year period who received first a non-rectal triage temperature and then a subsequent rectal temperature.RESULTS:The mean difference in temperatures between the initial temperature and the rectal temperature was 1.3 °F(P<0.001),with 25.9%of the patients having higher rectal temperatures >2°F,and 5.0%having higher rectal temperatures >4 °F.The mean difference among the patients who received oral,axillary,and temporal temperatures was 1.2 °F(PO.001),1.8 °F(PO.001),and 1.2 °F(P<0.001) respectively.About 18.1%of the patients were initially afebrile and found to be febrile by rectal temperature,with an average difference of 2.5 °F(P<0.001).These patients had a higher rate of admission(61.4%,P<0.005),and were more likely to be admitted to the hospital for a higher level of care,such as an intensive care unit,when compared with the full cohort(12.5%vs.5.8%,P<0.005).CONCLUSIONS:There are significant differences between rectal temperatures and noninvasive triage temperatures in this emergency department cohort.In almost one in five patients,fever was missed by triage temperature.
文摘Thamnocalamus falconeri, Hook.f. ex Munro.,an important bamboo species belonging to the family Poaceae, locally known as Ringal, occurs in the hills of Uttarakhand, India. This species has been traditionally exploited by local communities to support their livelihoods.Increasing needs of the hill villages impose unsustainable pressure on natural stands of Ringal in the Uttarakhand hills and forests have been degraded. The long history of excessive cutting of Ringal from natural forests and the lack of replanting threaten villager livelihoods. Replanting is required to conserve the species. We propose a protocol for generation of planting material through axillary bud proliferation for multiplication and conservation of this species. We collected offsets/rhizomes from a natural stand of T. falconeri in the Chopta Mandal areas（Chamoli district, India）. These were planted at sites of varied elevation and fresh single nodal segments were collected from them as explants. Different sterilization treatments were assessed to combat contamination. Among these, treatment of 0.1 %Hg Cl2 followed by 5 % Na OCl, proved best. Among two cytokinin treatments, viz. BAP and Kinetin, singly or in combination, BAP alone（5 mg L-1） proved superior and resulted in 100 % bud break. BAP-supplemented MS media yielded maximum vigorous shoot formation（90 %）and maximum number of shoots（8.9）. Subculturing of shoots on the same medium with similar BAP treatment（5 mg L-1BAP） enabled continuous production of healthy shoots at similar frequency. Maximum rooting（100 %）was recorded on half-strength MS medium supplemented with 5 mg L-1IBA. Micropropagated plants were hardened and acclimatized in soil mixture（2：1：1） and then transplanted to field sites（Magra, Uttarakhand, 1,834 m）.Eight to ten months after field transplantation we recorded100 % survival of transplanted material. This micropropagation protocol could be used successfully for raising a stock of genetically homogenous plant material in bulk for field plantations and for conservation of the species.
