OBJECTIVE To identify potential cell signaling pathways and protein targets of the active compound isolated from Atracylodes lancea "atractylodin" in cholangiocarcinoma,using proteomics approach.METHODS The ...OBJECTIVE To identify potential cell signaling pathways and protein targets of the active compound isolated from Atracylodes lancea "atractylodin" in cholangiocarcinoma,using proteomics approach.METHODS The holangiocar-cinoma cell line was exposed with atractylodin for 3 and 6 h and the proteins from both intra-and extra-cellular components were extracted.The extract proteins were separated by SDS-PAGE and digested with trypsin.The LC-MS/MS was applied to identify proteins.Signaling pathways and protein expression were analyzed by MASCOT and STITCH software.RESULTS A total of 4,323 and 4,318 proteins were identified from intra-and extracellular components,respectively.Six intracellular proteins were linked with the signaling pathways(apoptosis,cell cycle control,and PI3K-AKT).Four extracellular proteins were linked with the signaling pathways(NF-κB and PI3K-AKT).CONCLUSION All these proteins will further study to confirm the link to the anticholangiocarcinoma ac.tivity of actractylodin.展开更多
OBJECTIVE To formulate atractylodin-loaded poly(lactic-co-glycolic acid)(PLGA)nanoparticles and characterize the prepared nanoparticle formulation.METHODS The nanoparticle formulation was developed using solvent displ...OBJECTIVE To formulate atractylodin-loaded poly(lactic-co-glycolic acid)(PLGA)nanoparticles and characterize the prepared nanoparticle formulation.METHODS The nanoparticle formulation was developed using solvent displacement method.The encapsulation and loading efficiency were characterized and particle size,and zeta potential were determined by dynamic light scattering technique.Drug release was assessed in vitro.RESULTS The size(mean±SD of diameter) of the prepared atractylodin-loaded PLGA nanoparticles were(161.27 ± 1.87)nm with narrow size distribution(mean PDI:0.068±0.015) and zeta potential(28.83±0.35)mV.The encapsulation and loading efficiency were(48.31±0.83)% and(2.15±0.04)%,respectively.Drug release from atractylodin-loaded PLGA nanoparticles was observed up to(87.70±0.47)% in 72 h with biphasic manner.Moreover,the nanoparticles were found to be freely dispersible in water without aggregation.CONCLUSION Results suggest that PLGA nanoparticles may be used as an effective drug delivery system for atractylodin.The anti-cholangiocarcinoma activity of this nanoparticle formulation is required.展开更多
目的采用HPLC法研究不同配比苍术-玄参药对中哈巴苷、哈巴俄苷、安格洛苷C、苍术素溶出率的变化规律。方法分析采用Phenomenex Luna C_(18)色谱柱(250 mm×4.6 mm,5.0μm);流动相为乙腈-水,梯度洗脱;体积流量1.0 m L/min;检测波长21...目的采用HPLC法研究不同配比苍术-玄参药对中哈巴苷、哈巴俄苷、安格洛苷C、苍术素溶出率的变化规律。方法分析采用Phenomenex Luna C_(18)色谱柱(250 mm×4.6 mm,5.0μm);流动相为乙腈-水,梯度洗脱;体积流量1.0 m L/min;检测波长210 nm(哈巴苷)、280 nm(哈巴俄苷、安格洛苷C、苍术素);柱温25℃。结果当药对配伍比例为2∶1时,4种成分的总溶出率最高,而为1∶2时最低。结论哈巴苷和安格洛苷C的溶出率随苍术比例的增加而升高,而哈巴苷的溶出率和哈巴俄苷可能成反比关系。展开更多
基金supported by Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma,Chulabhorn International College of Medicine,Thammasat University The National Research University Project of Thailand(NRU)
文摘OBJECTIVE To identify potential cell signaling pathways and protein targets of the active compound isolated from Atracylodes lancea "atractylodin" in cholangiocarcinoma,using proteomics approach.METHODS The holangiocar-cinoma cell line was exposed with atractylodin for 3 and 6 h and the proteins from both intra-and extra-cellular components were extracted.The extract proteins were separated by SDS-PAGE and digested with trypsin.The LC-MS/MS was applied to identify proteins.Signaling pathways and protein expression were analyzed by MASCOT and STITCH software.RESULTS A total of 4,323 and 4,318 proteins were identified from intra-and extracellular components,respectively.Six intracellular proteins were linked with the signaling pathways(apoptosis,cell cycle control,and PI3K-AKT).Four extracellular proteins were linked with the signaling pathways(NF-κB and PI3K-AKT).CONCLUSION All these proteins will further study to confirm the link to the anticholangiocarcinoma ac.tivity of actractylodin.
基金supported by Center of Excellence in Pharmacology and Molecular Biology of Malaria and Cholangiocarcinoma,Chulabhorn International College of Medicine,Thammasat University the National Research University Project of Thailand(NRU)
文摘OBJECTIVE To formulate atractylodin-loaded poly(lactic-co-glycolic acid)(PLGA)nanoparticles and characterize the prepared nanoparticle formulation.METHODS The nanoparticle formulation was developed using solvent displacement method.The encapsulation and loading efficiency were characterized and particle size,and zeta potential were determined by dynamic light scattering technique.Drug release was assessed in vitro.RESULTS The size(mean±SD of diameter) of the prepared atractylodin-loaded PLGA nanoparticles were(161.27 ± 1.87)nm with narrow size distribution(mean PDI:0.068±0.015) and zeta potential(28.83±0.35)mV.The encapsulation and loading efficiency were(48.31±0.83)% and(2.15±0.04)%,respectively.Drug release from atractylodin-loaded PLGA nanoparticles was observed up to(87.70±0.47)% in 72 h with biphasic manner.Moreover,the nanoparticles were found to be freely dispersible in water without aggregation.CONCLUSION Results suggest that PLGA nanoparticles may be used as an effective drug delivery system for atractylodin.The anti-cholangiocarcinoma activity of this nanoparticle formulation is required.
文摘目的采用HPLC法研究不同配比苍术-玄参药对中哈巴苷、哈巴俄苷、安格洛苷C、苍术素溶出率的变化规律。方法分析采用Phenomenex Luna C_(18)色谱柱(250 mm×4.6 mm,5.0μm);流动相为乙腈-水,梯度洗脱;体积流量1.0 m L/min;检测波长210 nm(哈巴苷)、280 nm(哈巴俄苷、安格洛苷C、苍术素);柱温25℃。结果当药对配伍比例为2∶1时,4种成分的总溶出率最高,而为1∶2时最低。结论哈巴苷和安格洛苷C的溶出率随苍术比例的增加而升高,而哈巴苷的溶出率和哈巴俄苷可能成反比关系。
