The human promyelocytic cell line HL-60 overexpresses the c-myc protooncogene. Plasmid pDACx carrying antisense human c-myc DNA and neo gene was introduced into HL-60 cells with lipofectin reagent. Upon DNA entering t...The human promyelocytic cell line HL-60 overexpresses the c-myc protooncogene. Plasmid pDACx carrying antisense human c-myc DNA and neo gene was introduced into HL-60 cells with lipofectin reagent. Upon DNA entering the tar-geted celis and expression of antisense transcripts to c-myc, C-MYC protein level, cell proliferation and colony-forming potentiality were all definitely inhibited.展开更多
Purpose: To explore whether the antisense RNA ofMHC-Ⅱ gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB)cells.The authors transduced the antisense RNA ofMHC-Ⅱ gene int...Purpose: To explore whether the antisense RNA ofMHC-Ⅱ gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB)cells.The authors transduced the antisense RNA ofMHC-Ⅱ gene into CD34 CB stem/progenitor cells.Methods: A retroviral vector encoding antisense RNA ofHLA-DR gene was constructed by molecular cloningtechnique and transducted into packaging cells line PA317by lipofectin. The high titer, helper-free,展开更多
To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon...To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon 2 was ligated into retroviral vector pDOR-neoin reverse direction. This recombinant plasmid展开更多
Objectives: To construct retroviral vectorsexpressing antisense polymeric RNAs targeted at the coresequence (+13-+47) of HIV-l TAR RNA, and to testthe anti-HIV properties of this construct in transducedCD4^+T cells. M...Objectives: To construct retroviral vectorsexpressing antisense polymeric RNAs targeted at the coresequence (+13-+47) of HIV-l TAR RNA, and to testthe anti-HIV properties of this construct in transducedCD4^+T cells. Methods: The polymeric TAR-Core DNAwas amplified by the"self-primers-template PCR"展开更多
The insulin-like growth factor l (IGF-1) iscommonly expressed in most of tumor cells and plays arole in the growth and development of animal andhuman tumors. Tumor cells transfected with a vectorencoding antisense IGF...The insulin-like growth factor l (IGF-1) iscommonly expressed in most of tumor cells and plays arole in the growth and development of animal andhuman tumors. Tumor cells transfected with a vectorencoding antisense IGF-l cDNA transcriptionalcassette driven by the mouse展开更多
Objective: Overexpression of the membraneepidermal growth factor receptor (EGFR) in humantumor cells predicts for poor prognosis. Hence, weexperimentally investigated the effects of humanEGFR to the malignant phenotyp...Objective: Overexpression of the membraneepidermal growth factor receptor (EGFR) in humantumor cells predicts for poor prognosis. Hence, weexperimentally investigated the effects of humanEGFR to the malignant phenotype in humen breastcancer cell line MDA-MB-231 and humannasopharyngeal carcinoma cell line CNE-2. Methods:展开更多
Previous studies have shown that the bcl-2protooncogene encodes a mitochondrial protein thatpromotes cell survival by blocking programmed celldeath. High levels of bcl-2 expression were found inmurine myeloid leukemic...Previous studies have shown that the bcl-2protooncogene encodes a mitochondrial protein thatpromotes cell survival by blocking programmed celldeath. High levels of bcl-2 expression were found inmurine myeloid leukemic cell lines resistant to apoptosisinduced by variety of agents. In addition artificialelevation of bcl-2 expression in a human pre-B leukemiccell line resulted in enhanced resistance to chemo-therapcutic drugs. We reported here on the effect ofbcl-2 antisense phosphorothiate展开更多
A plasmid expressing antisense MDRl cDNAsegment was introduced into KB<sub>v200</sub> which inductedmultiple drugs to VCT （vincristine, 175 fold-resistancehigher than that in original KB cells） and ADM（...A plasmid expressing antisense MDRl cDNAsegment was introduced into KB<sub>v200</sub> which inductedmultiple drugs to VCT （vincristine, 175 fold-resistancehigher than that in original KB cells） and ADM（adriamycin, 14. 5 fold） resulting over-expression ofMDRl. We used the primers for antisense RNA asfollowing: upstream 5′OGAATTCTGAAACCTGTAAGCAGCAACC 3′: downstream展开更多
Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polym...Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polymerasessynthesize DNA in the 5’ to 3’ direction and require aRNA primer for initiation, telomeric DNA could belost at chromosome ends unless the termini arespecifically extended by telomerase. Telomerase is aspecialized ribonucleoprotein polymerase that containsan integral RNA with a short template element展开更多
Antisense oligonucleotides have been shown to beunique drugs that achieve their effect by targetingmRNA with which they can hybridize and specificallyblock gene expression, offering the possibility ofspecific, rationa...Antisense oligonucleotides have been shown to beunique drugs that achieve their effect by targetingmRNA with which they can hybridize and specificallyblock gene expression, offering the possibility ofspecific, rational drug design. However, there havebeen many concerns that have limited enthusiasm forthe development of these drugs from the preclinical展开更多
During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedth...During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedthe sites of the rearrangement by v-erbB PCR genediagnosis of MDS and confirmed their etiogenicsignificance by the follow-up of MDS cases. These resultsprovided 2 target sequences of c-erbB.The展开更多
Over the past 10 years adoptive immunotherapieshave been developed for cancer treatment. Cytotoxic Tlymphocytes (CTL) play a major role in host antitumorimmune response. The perforin and Fas ligand (Fas-L)pathways whi...Over the past 10 years adoptive immunotherapieshave been developed for cancer treatment. Cytotoxic Tlymphocytes (CTL) play a major role in host antitumorimmune response. The perforin and Fas ligand (Fas-L)pathways which were two major mechanisms are res-ponsible for tumor cell death by CTLs. A major obstacleto the application of adoptive imunotherapy in thetreatment of human malignancy has been the inability展开更多
The essence of gene therapy is to placefunctioning genes into a patient’s cells for therapeuticpurposes. Gene therapy may be employed to ①enhance anti-tumor immune responses; ②
With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β conv...With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β converting enzyme (ICE) gene, ahomologue of the Caenorhabiditis elegans ce1l death geneced-3, has been identified as a key inducer of apoptosis inmammalian cells. However, no study of ICE genetherapy on cancer has been reported up to now. Weconstructed recombinant retroviral vectors with展开更多
文摘The human promyelocytic cell line HL-60 overexpresses the c-myc protooncogene. Plasmid pDACx carrying antisense human c-myc DNA and neo gene was introduced into HL-60 cells with lipofectin reagent. Upon DNA entering the tar-geted celis and expression of antisense transcripts to c-myc, C-MYC protein level, cell proliferation and colony-forming potentiality were all definitely inhibited.
文摘Purpose: To explore whether the antisense RNA ofMHC-Ⅱ gene could inodulate the expression of HLA-DRgene and reduce the immunogenicity of cord blood (CB)cells.The authors transduced the antisense RNA ofMHC-Ⅱ gene into CD34 CB stem/progenitor cells.Methods: A retroviral vector encoding antisense RNA ofHLA-DR gene was constructed by molecular cloningtechnique and transducted into packaging cells line PA317by lipofectin. The high titer, helper-free,
文摘To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon 2 was ligated into retroviral vector pDOR-neoin reverse direction. This recombinant plasmid
文摘Objectives: To construct retroviral vectorsexpressing antisense polymeric RNAs targeted at the coresequence (+13-+47) of HIV-l TAR RNA, and to testthe anti-HIV properties of this construct in transducedCD4^+T cells. Methods: The polymeric TAR-Core DNAwas amplified by the"self-primers-template PCR"
文摘The insulin-like growth factor l (IGF-1) iscommonly expressed in most of tumor cells and plays arole in the growth and development of animal andhuman tumors. Tumor cells transfected with a vectorencoding antisense IGF-l cDNA transcriptionalcassette driven by the mouse
文摘Objective: Overexpression of the membraneepidermal growth factor receptor (EGFR) in humantumor cells predicts for poor prognosis. Hence, weexperimentally investigated the effects of humanEGFR to the malignant phenotype in humen breastcancer cell line MDA-MB-231 and humannasopharyngeal carcinoma cell line CNE-2. Methods:
文摘Previous studies have shown that the bcl-2protooncogene encodes a mitochondrial protein thatpromotes cell survival by blocking programmed celldeath. High levels of bcl-2 expression were found inmurine myeloid leukemic cell lines resistant to apoptosisinduced by variety of agents. In addition artificialelevation of bcl-2 expression in a human pre-B leukemiccell line resulted in enhanced resistance to chemo-therapcutic drugs. We reported here on the effect ofbcl-2 antisense phosphorothiate
文摘A plasmid expressing antisense MDRl cDNAsegment was introduced into KB<sub>v200</sub> which inductedmultiple drugs to VCT （vincristine, 175 fold-resistancehigher than that in original KB cells） and ADM（adriamycin, 14. 5 fold） resulting over-expression ofMDRl. We used the primers for antisense RNA asfollowing: upstream 5′OGAATTCTGAAACCTGTAAGCAGCAACC 3′: downstream
文摘Human chromosomes terminate with severalkilobases of the simple telomere repeat (TTAGGG) n.Telomeres protect the chromosomes from DNAdegradation, end-to-end fusion, rearrangements andchromosome losses. Since DNA polymerasessynthesize DNA in the 5’ to 3’ direction and require aRNA primer for initiation, telomeric DNA could belost at chromosome ends unless the termini arespecifically extended by telomerase. Telomerase is aspecialized ribonucleoprotein polymerase that containsan integral RNA with a short template element
文摘Antisense oligonucleotides have been shown to beunique drugs that achieve their effect by targetingmRNA with which they can hybridize and specificallyblock gene expression, offering the possibility ofspecific, rational drug design. However, there havebeen many concerns that have limited enthusiasm forthe development of these drugs from the preclinical
文摘During the past ten years. our molecular studies haddemonstrated that myelodysplasia of MDS bone marrowwas associated with the rearrangement/amplification ofc-erbB and deletion/ inactivation of c-erbA and identifiedthe sites of the rearrangement by v-erbB PCR genediagnosis of MDS and confirmed their etiogenicsignificance by the follow-up of MDS cases. These resultsprovided 2 target sequences of c-erbB.The
文摘Over the past 10 years adoptive immunotherapieshave been developed for cancer treatment. Cytotoxic Tlymphocytes (CTL) play a major role in host antitumorimmune response. The perforin and Fas ligand (Fas-L)pathways which were two major mechanisms are res-ponsible for tumor cell death by CTLs. A major obstacleto the application of adoptive imunotherapy in thetreatment of human malignancy has been the inability
文摘The essence of gene therapy is to placefunctioning genes into a patient’s cells for therapeuticpurposes. Gene therapy may be employed to ①enhance anti-tumor immune responses; ②
文摘With deep research into apoptosis recently,malignancy has been recognized to result not only fromenhanced cell proliferation but also from decreasedphysiological programmed cell death (apoptosis).Interleukin-l β converting enzyme (ICE) gene, ahomologue of the Caenorhabiditis elegans ce1l death geneced-3, has been identified as a key inducer of apoptosis inmammalian cells. However, no study of ICE genetherapy on cancer has been reported up to now. Weconstructed recombinant retroviral vectors with
