The effects of the microspore developmental stage,hormones and culture condition on anther in vitro culture of lily（Lilium spp.） were discussed.The results showed that when the flower buds were about 23-26 mm long,t...The effects of the microspore developmental stage,hormones and culture condition on anther in vitro culture of lily（Lilium spp.） were discussed.The results showed that when the flower buds were about 23-26 mm long,the microspores were at the uninucleate stage which was suitable for culture and the culture under the darkness would promote the callus induction of anther.The induction frequency could reach 42.5% in the optimized medium which was MS＋[6-BA（0.5）＋KT（2.0）＋2,4-D（1.0）] mg·L-1.The rate of callus differentiation could reach 31.57% in the optimized medium which was MS＋ NAA（1.5,2.0） mg·L-1.展开更多
Significant differences in the effects of aminoprophosmethyl(APM) on ather culture were observed among 6 Australian cultivars in rice.APM had improved anther culture efficienncy in 5 cultivars,but decreased the induce...Significant differences in the effects of aminoprophosmethyl(APM) on ather culture were observed among 6 Australian cultivars in rice.APM had improved anther culture efficienncy in 5 cultivars,but decreased the induce rate of callus in one.展开更多
ADP-ribosylation factor(ARF)is considered asthe family of small GTP binding proteins,withmolecular mass around 21KD.It has beenknown that ARF plays important roles in cells toregulate membrane traffic and structure an...ADP-ribosylation factor(ARF)is considered asthe family of small GTP binding proteins,withmolecular mass around 21KD.It has beenknown that ARF plays important roles in cells toregulate membrane traffic and structure andintracellular signal transduction system in yeastand mammalian.Although ARFs have展开更多
以采集自贵州省都匀市小围寨镇茶农村的线叶水芹雄性不育植株ODDBY001及可育植株ODDKY001为试材,对不育株和可育株的植株形态、花器官结构性状、育性差异进行分析。结果表明:ODDBY001为线叶水芹种(Oenanthe dielsii de Boiss)雄性不育,...以采集自贵州省都匀市小围寨镇茶农村的线叶水芹雄性不育植株ODDBY001及可育植株ODDKY001为试材,对不育株和可育株的植株形态、花器官结构性状、育性差异进行分析。结果表明:ODDBY001为线叶水芹种(Oenanthe dielsii de Boiss)雄性不育,其花丝萎缩,花药较小,无花粉粒,花柱伸长且外露不明显;而可育植株ODDKY001的花丝伸长,花药饱满,含有大量的花粉粒,花柱较短。遗传分析表明,ODDBY001和ODDKY001线粒体基因组中atp6在5′端发生严重缺失,育性类型分别为S(rr)和S(Rr)。展开更多
基金Supported by Program of Postdoctoral Funds Project of Heilongjiang Province (LBH-Z08259)Program for Innovative Research Team of Northeast Agricultural University (CXZ004)
文摘The effects of the microspore developmental stage,hormones and culture condition on anther in vitro culture of lily（Lilium spp.） were discussed.The results showed that when the flower buds were about 23-26 mm long,the microspores were at the uninucleate stage which was suitable for culture and the culture under the darkness would promote the callus induction of anther.The induction frequency could reach 42.5% in the optimized medium which was MS＋[6-BA（0.5）＋KT（2.0）＋2,4-D（1.0）] mg·L-1.The rate of callus differentiation could reach 31.57% in the optimized medium which was MS＋ NAA（1.5,2.0） mg·L-1.
文摘Significant differences in the effects of aminoprophosmethyl(APM) on ather culture were observed among 6 Australian cultivars in rice.APM had improved anther culture efficienncy in 5 cultivars,but decreased the induce rate of callus in one.
文摘ADP-ribosylation factor(ARF)is considered asthe family of small GTP binding proteins,withmolecular mass around 21KD.It has beenknown that ARF plays important roles in cells toregulate membrane traffic and structure andintracellular signal transduction system in yeastand mammalian.Although ARFs have
文摘以采集自贵州省都匀市小围寨镇茶农村的线叶水芹雄性不育植株ODDBY001及可育植株ODDKY001为试材,对不育株和可育株的植株形态、花器官结构性状、育性差异进行分析。结果表明:ODDBY001为线叶水芹种(Oenanthe dielsii de Boiss)雄性不育,其花丝萎缩,花药较小,无花粉粒,花柱伸长且外露不明显;而可育植株ODDKY001的花丝伸长,花药饱满,含有大量的花粉粒,花柱较短。遗传分析表明,ODDBY001和ODDKY001线粒体基因组中atp6在5′端发生严重缺失,育性类型分别为S(rr)和S(Rr)。
