Baicalin(BA)is commonly used to treat inflammatory diseases and shows anti-inflammatory effects.The present study aimed to evaluate the analgesic and anti-inflammatory activities of BA both in vitro and in vivo.In ani...Baicalin(BA)is commonly used to treat inflammatory diseases and shows anti-inflammatory effects.The present study aimed to evaluate the analgesic and anti-inflammatory activities of BA both in vitro and in vivo.In animal models,acetic acid-induced writhing was used to assess analgesic activity.In addition,a variety of tests including xylene-induced ear edema test,minimum inhibitory concentration(MIC)assays and acetic acid-induced peritoneal capillary hyperpermeability test were used to evaluate antiinflammatory activity of BA.BA at 0.2 and 0.1 mg·mL-1 doses expressed analgesic as well as anti-inflammatory activities in mice.In acetic acid induced writhing test,BA applying three times,twice and once a day significantly inhibited the acetic acid-induced writhing response within 15 min by 7.80%(*p<0.05),7.50%(**p<0.01)and 6.25%(**p<0.01).In xylene-induced ear edema test,BA at 0.2,0.1 and 0.05 mg·mL-1 decreased ear edema by 45.50%(**p<0.01),15.20%(*p<0.05)and 9.10%(*p<0.05).In acetic acidinduced peritoneal capillary hyperpermeability test,BA exhibited significant inhibition(*p<0.05 versus control)of inflammation.In MIC assays,the MIC values of baicalin for S.aureus and Escherichia coli were 125 mg•mL-1,and the MIC values for the control bacteria ATCC25922 and ATCC25923 were 62.5 mg·mL-1.These findings suggested baicalin might contain analgesic and antiinflammatory agents which supported its use in traditional medicine.展开更多
基金Supported by the National Natural Science Foundation of China(31772801)Academic Backbone Project of Northeast Agricultural University(18XG23)。
文摘Baicalin(BA)is commonly used to treat inflammatory diseases and shows anti-inflammatory effects.The present study aimed to evaluate the analgesic and anti-inflammatory activities of BA both in vitro and in vivo.In animal models,acetic acid-induced writhing was used to assess analgesic activity.In addition,a variety of tests including xylene-induced ear edema test,minimum inhibitory concentration(MIC)assays and acetic acid-induced peritoneal capillary hyperpermeability test were used to evaluate antiinflammatory activity of BA.BA at 0.2 and 0.1 mg·mL-1 doses expressed analgesic as well as anti-inflammatory activities in mice.In acetic acid induced writhing test,BA applying three times,twice and once a day significantly inhibited the acetic acid-induced writhing response within 15 min by 7.80%(*p<0.05),7.50%(**p<0.01)and 6.25%(**p<0.01).In xylene-induced ear edema test,BA at 0.2,0.1 and 0.05 mg·mL-1 decreased ear edema by 45.50%(**p<0.01),15.20%(*p<0.05)and 9.10%(*p<0.05).In acetic acidinduced peritoneal capillary hyperpermeability test,BA exhibited significant inhibition(*p<0.05 versus control)of inflammation.In MIC assays,the MIC values of baicalin for S.aureus and Escherichia coli were 125 mg•mL-1,and the MIC values for the control bacteria ATCC25922 and ATCC25923 were 62.5 mg·mL-1.These findings suggested baicalin might contain analgesic and antiinflammatory agents which supported its use in traditional medicine.
