OBJECTIVE To evaluate the in vivo anti-inflammatory and allergic rhinitis(AR)alleviating effect as well as in vitro antimicrobial activities of Artemisia ordosica Krasch.(AOK)extracts to verify its ethno-medicinal cla...OBJECTIVE To evaluate the in vivo anti-inflammatory and allergic rhinitis(AR)alleviating effect as well as in vitro antimicrobial activities of Artemisia ordosica Krasch.(AOK)extracts to verify its ethno-medicinal claims.METHODS Crude extracts(methanol/95%-ethanol/ethyl acetate)of AOK root/stem/leaf and fractions(petroleum ether/ethyl acetate/n-butanol/aqueous)of AOK root extractwere prepared.Xylene-induced ear swelling model in mouse and ovalbumin(OVA)-induced AR model in guinea pig were established.Ear swelling degrees of mice were measured.The numbers of rubbing movement and sneezes of guinea pigs were counted to evaluate the symptoms of AR.The serum levels of histamine,INF-γ,IL-2/4/10,and VCAM-1 were measured by ELISA assay.The histological changes of nasal mucosa were investigated by light microscope after H&E staining.Antimicrobial activities of AOK extracts were also tested.LC-MS/MS analysis was performed to characterize the constituents of active extract and molecular docking was conducted to predict the biological mechanism.RESULTS In ear-swelling model,extract(100.00 mg·kg^-1)from the ethyl acetate layer of 95%ethanol(100.00 mg·kg^-1)showed better swelling inhibition in mice than positive control(dexamethasone,191.91 mg·kg^-1).In AR model,extract from the ethyl acetate layer of 95%ethanol significantly alleviated the AR symp⁃toms in guinea pigs,decreased the serum levels of histamine,INF-γ,IL-2/4/10,and VCAM-1,and reduced the infiltration of eosinophil in nasal mucosa.For Staphylococcus aureus,the ethyl acetate extract of AOK stem showed the highest inhibi⁃tion(MIC=1.25 g·L^-1),for Escherichia coli,n-butanol layer of 95%ethanol extract of AOK root showed the highest inhibi⁃tion(MIC=15.00 g·L^-1),for Candida glabrata,95%-ethanol extract of AOK stem showed the best inhibition(MIC=0.064 g·L^-1),while ethyl acetate and n-butanol layers showed similar inhibition on MRSA(MIC=7.50 g·L-1).LC-MS/MS characteriza⁃tion showed that dicaffeoylquinic acids account for more than 30%of ethyl acetate layer of AOK extract.Dicaffeoylquinic acids bind with histamine-1 receptor with high affinities and interesting modes.CONCLUSION Extracts from AOK had interesting anti-inflammatory activity in mice,alleviating effect against OVA-induced AR in guinea pigs,and antimicrobial activities in vitro,which support the ethno-medicinal use of it.The main constituents in ethyl acetate layer of AOK root extract are dicaffeoylquinic acids and could bind with histamine-1 receptor well.These findings highlighted the impor⁃tance of natural product chemistry study of AOK.展开更多
Aim To study the effect and mechanism of Chlorogenic acid in allergic rhinitis （AR） mice. MethodsAR was induced in BALB/c mice according to a published method with ovalbumin （OVA）. The AR model was de- veloped by ...Aim To study the effect and mechanism of Chlorogenic acid in allergic rhinitis （AR） mice. MethodsAR was induced in BALB/c mice according to a published method with ovalbumin （OVA）. The AR model was de- veloped by intraperitoneal injection of adjuvant suspension on every other day for 7 times. Adjuvant suspension in- clude 50 μg of OVA and 2rag of AI（OH） s, which were combined in 2ml of normal saline （NS）. Then, the mice received a nasal drip of 10% OVA daily for 10days, and in normal control were dripped NS. After administration with 50 mg· kg^-1, 100 mg · kg^-1 and 200 mg · kg^-1 of Chlorogenic acid for 30min, The mice were attacked lo- cally for 10 days. We observed behavior analysis, evaluated the weight of nosal draingage, related the index of thy- mus and spleen, determined the effect of Chlorogenic acid on the contents of cytokines, IgE and histamine by ELISA in AR mice and analyzed the mRNA expression of inflammatory cytokines （IL-4, IL-5, IL-10, IFN-y） in the nasal mucosa tissues samples by RT-PCR. Results Chlorogenic acid decreased the number of nose-scratching events in 10min, the weight of nosal draingage, the index of spleen, the content of IL-4 in nasal lavage fluid（NLF） and the contents of cytokines, IgE and histamine in serum; while increased the index of thymus, the content of IFN-γ in NLF, the contents of IFN-γ in serum and the rate of IFN-γ/IL-4 ; The data showed a statistically signifi- cant up-regulation of IFN-γ mRNA expressions and down-regulation of IL-4mRNA, IL-5mRNA, IL-10mRNA ex- pressions after adiminstration of Chlorogenic acid. Conclusion Chlorogenic acid might exert the effect in AR mice through regulating the balance of Thl and Th2.展开更多
基金Science Foundation of Inner Mongolia(2017MS0870)Science and Technology Planning Project of Inner Mongolia(201702139)
文摘OBJECTIVE To evaluate the in vivo anti-inflammatory and allergic rhinitis(AR)alleviating effect as well as in vitro antimicrobial activities of Artemisia ordosica Krasch.(AOK)extracts to verify its ethno-medicinal claims.METHODS Crude extracts(methanol/95%-ethanol/ethyl acetate)of AOK root/stem/leaf and fractions(petroleum ether/ethyl acetate/n-butanol/aqueous)of AOK root extractwere prepared.Xylene-induced ear swelling model in mouse and ovalbumin(OVA)-induced AR model in guinea pig were established.Ear swelling degrees of mice were measured.The numbers of rubbing movement and sneezes of guinea pigs were counted to evaluate the symptoms of AR.The serum levels of histamine,INF-γ,IL-2/4/10,and VCAM-1 were measured by ELISA assay.The histological changes of nasal mucosa were investigated by light microscope after H&E staining.Antimicrobial activities of AOK extracts were also tested.LC-MS/MS analysis was performed to characterize the constituents of active extract and molecular docking was conducted to predict the biological mechanism.RESULTS In ear-swelling model,extract(100.00 mg·kg^-1)from the ethyl acetate layer of 95%ethanol(100.00 mg·kg^-1)showed better swelling inhibition in mice than positive control(dexamethasone,191.91 mg·kg^-1).In AR model,extract from the ethyl acetate layer of 95%ethanol significantly alleviated the AR symp⁃toms in guinea pigs,decreased the serum levels of histamine,INF-γ,IL-2/4/10,and VCAM-1,and reduced the infiltration of eosinophil in nasal mucosa.For Staphylococcus aureus,the ethyl acetate extract of AOK stem showed the highest inhibi⁃tion(MIC=1.25 g·L^-1),for Escherichia coli,n-butanol layer of 95%ethanol extract of AOK root showed the highest inhibi⁃tion(MIC=15.00 g·L^-1),for Candida glabrata,95%-ethanol extract of AOK stem showed the best inhibition(MIC=0.064 g·L^-1),while ethyl acetate and n-butanol layers showed similar inhibition on MRSA(MIC=7.50 g·L-1).LC-MS/MS characteriza⁃tion showed that dicaffeoylquinic acids account for more than 30%of ethyl acetate layer of AOK extract.Dicaffeoylquinic acids bind with histamine-1 receptor with high affinities and interesting modes.CONCLUSION Extracts from AOK had interesting anti-inflammatory activity in mice,alleviating effect against OVA-induced AR in guinea pigs,and antimicrobial activities in vitro,which support the ethno-medicinal use of it.The main constituents in ethyl acetate layer of AOK root extract are dicaffeoylquinic acids and could bind with histamine-1 receptor well.These findings highlighted the impor⁃tance of natural product chemistry study of AOK.
文摘Aim To study the effect and mechanism of Chlorogenic acid in allergic rhinitis （AR） mice. MethodsAR was induced in BALB/c mice according to a published method with ovalbumin （OVA）. The AR model was de- veloped by intraperitoneal injection of adjuvant suspension on every other day for 7 times. Adjuvant suspension in- clude 50 μg of OVA and 2rag of AI（OH） s, which were combined in 2ml of normal saline （NS）. Then, the mice received a nasal drip of 10% OVA daily for 10days, and in normal control were dripped NS. After administration with 50 mg· kg^-1, 100 mg · kg^-1 and 200 mg · kg^-1 of Chlorogenic acid for 30min, The mice were attacked lo- cally for 10 days. We observed behavior analysis, evaluated the weight of nosal draingage, related the index of thy- mus and spleen, determined the effect of Chlorogenic acid on the contents of cytokines, IgE and histamine by ELISA in AR mice and analyzed the mRNA expression of inflammatory cytokines （IL-4, IL-5, IL-10, IFN-y） in the nasal mucosa tissues samples by RT-PCR. Results Chlorogenic acid decreased the number of nose-scratching events in 10min, the weight of nosal draingage, the index of spleen, the content of IL-4 in nasal lavage fluid（NLF） and the contents of cytokines, IgE and histamine in serum; while increased the index of thymus, the content of IFN-γ in NLF, the contents of IFN-γ in serum and the rate of IFN-γ/IL-4 ; The data showed a statistically signifi- cant up-regulation of IFN-γ mRNA expressions and down-regulation of IL-4mRNA, IL-5mRNA, IL-10mRNA ex- pressions after adiminstration of Chlorogenic acid. Conclusion Chlorogenic acid might exert the effect in AR mice through regulating the balance of Thl and Th2.
文摘目的比较变应性鼻炎(allergic rhinitis,AR)患者接受标准化尘螨变应原疫苗皮下免疫治疗(subcutaneous immunotherapy,SCIT)和舌下免疫治疗(sublingual immunotherapy,SLIT)的疗效及对患者生活质量的改善程度。方法 96例尘螨过敏的中-重度持续性AR患者纳入本研究,分别采用SCIT和SLIT方案,完成2年的特异性免疫治疗。其中SCIT组46例、SLIT组50例。比较两组治疗前后症状评分(VAS评分)、药物用量评分以及疾病专用量表-鼻结膜炎生存质量调查问卷(rhinoconjunctivitis quality of life questionnaire,RQLQ)评分,评价各组的疗效和对患者生活质量的改善程度,并观察两组的不良反应发生率。结果治疗2年后,SCIT组和SLIT组患者的喷嚏、流涕、鼻塞和鼻痒症状以及鼻部症状总评分(VAS评分)、药物用量评分均明显下降,SCIT治疗对减少药物使用的效果较显著,同SLIT组比较,差异有统计学意义(P<0.05)。治疗后两组患者的RQLQ评分均有下降,尤其在鼻部症状和实际问题这2个维度评分下降明显,与其他维度相比,差异有统计学意义(P<0.05)。结论尘螨过敏的中-重度持续性AR患者采用SCIT或SLIT进行为期2年的特异性免疫治疗均能获得良好效果,改善患者生活质量的程度未见明显差异,但是SCIT治疗能减少药物的使用。
