Molasses wastewater was evaluated as substrate for biohydrogen production by anaerobic fermentation in a novel continuous mixed attached growth reactor ( CMAGR ) with aeration pretreated sludge attached onto granular ...Molasses wastewater was evaluated as substrate for biohydrogen production by anaerobic fermentation in a novel continuous mixed attached growth reactor ( CMAGR ) with aeration pretreated sludge attached onto granular activated carbon under continuous flow condition.It was indicated that the CMAGR system was operated at the conditions of influent COD of 2000～6000mg / L , hydraulic retention time ( HRT ) of 6hand temperature of 35 ℃ , when the pH value and oxidation-reduction potential ( ORP ) ranged from 4.16and-434 mV respectively , stable ethanol-type fermentation was formed with the sum of ethanol and acetate concentration ratio of 89.3%to the total liquid products after 40days operation.The H 2 content in biogas and chemical oxygen demand ( COD ) removal were estimated to be 46.6% and 13% , respectively.It was also investigated that the effects of organic loading rates ( OLRs ) on CMAGR hydrogen production system.It was found that hydrogen production yield increased from 3.72 mmol / hL to 12.51 mmol / hL as OLRs increased from 8 kg / m 3 d to 32 kg / m 3 d.The maximum hydrogen production rate of 12.51mmol / hL at a OLR of 32kg / m 3 d and the maximum hydrogen yield by substrate consumed was 130.57 mmol / mol happened at OLR of 16 kg / m 3 d.Greater pHs appeared to be favour to butyrate production and the maximum of 0.51mol / mol was obtained at pH of 4.14.However , ethanol / acetate ratio was greater than 1.1at pH fluctuated between 3.4 - 3.6and 4.1 - 4.4which indicated that these pHs were favour to ethanol type fermentation.Therefore , the continuous mixed attached growth reactor ( CMAGR ) could be a promising attached growth system for biohydrogen fermentation.展开更多
基金support from the National Hi-Tech R&D Program(863 Program)Ministry of Science & Technology,China(2006AA05Z109)+2 种基金Shanghai Science and Technology Bureau(071605122)Shanghai Education Committee(07ZZ156)GRAP09,Northeast Forestry University are gratefully acknowledged
文摘Molasses wastewater was evaluated as substrate for biohydrogen production by anaerobic fermentation in a novel continuous mixed attached growth reactor ( CMAGR ) with aeration pretreated sludge attached onto granular activated carbon under continuous flow condition.It was indicated that the CMAGR system was operated at the conditions of influent COD of 2000～6000mg / L , hydraulic retention time ( HRT ) of 6hand temperature of 35 ℃ , when the pH value and oxidation-reduction potential ( ORP ) ranged from 4.16and-434 mV respectively , stable ethanol-type fermentation was formed with the sum of ethanol and acetate concentration ratio of 89.3%to the total liquid products after 40days operation.The H 2 content in biogas and chemical oxygen demand ( COD ) removal were estimated to be 46.6% and 13% , respectively.It was also investigated that the effects of organic loading rates ( OLRs ) on CMAGR hydrogen production system.It was found that hydrogen production yield increased from 3.72 mmol / hL to 12.51 mmol / hL as OLRs increased from 8 kg / m 3 d to 32 kg / m 3 d.The maximum hydrogen production rate of 12.51mmol / hL at a OLR of 32kg / m 3 d and the maximum hydrogen yield by substrate consumed was 130.57 mmol / mol happened at OLR of 16 kg / m 3 d.Greater pHs appeared to be favour to butyrate production and the maximum of 0.51mol / mol was obtained at pH of 4.14.However , ethanol / acetate ratio was greater than 1.1at pH fluctuated between 3.4 - 3.6and 4.1 - 4.4which indicated that these pHs were favour to ethanol type fermentation.Therefore , the continuous mixed attached growth reactor ( CMAGR ) could be a promising attached growth system for biohydrogen fermentation.
