Gold nanoparticles(GNPs)have been extensively used in nanomedicine and neuroscience owing to their biological inertness,peculiar opto-electronic and physico-chemical features.However,the effect of GNPs shape on the ne...Gold nanoparticles(GNPs)have been extensively used in nanomedicine and neuroscience owing to their biological inertness,peculiar opto-electronic and physico-chemical features.However,the effect of GNPs shape on the neurophysiological properties of single neuron is still unclear.To tackle this issue,different shape GNPs(nanosphere,nanotriakisoctahedron and nanoflower)were synthesized to investigate the effect of GNPs on the voltage-dependent sodium channel and the action potential(AP)of hippocampal CA1 neurons in mice.The results indicated that GNPs inhibited the amplitudes of voltage-gated sodium current(I_(Na))and led to a hyperpolarizing shift in the voltage-dependence curve of both activation and inactivation of I_(Na).GNPs also increased neuronal excitability and altered some properties of AP.Moreover,most alterations in AP properties were observed in nanoflower GNPs treated CA1 neurons,suggesting that the neurotoxicity of gold nanoparticles is surface roughness-dependent.These results may provide a valuable direction in the clinical application of GNPs.展开更多
AP and VP were measured in cucumbers under water sufficiency and water stress. The results indicated that, the AP would be evoked by electrical impulse, for water-stressed cucumber, its amplitude could reached more th...AP and VP were measured in cucumbers under water sufficiency and water stress. The results indicated that, the AP would be evoked by electrical impulse, for water-stressed cucumber, its amplitude could reached more than 40 mV which was obviously greater than that (about 10-20 mV) of plant under well-watered, and no VP came out. Along with the intensity of light increased, the VP appeared going-up trend, and accompanied by evidently spiking electrical signal, for plant under water stress, the VP increased more clearly, but the change of spiking amplitude of AP (about 3 mV) was rather smaller than that (8-10 mV) of plant under well-watered.展开更多
基金Project(LY19C090004)supported by the Natural Science Foundation of Zhejiang Province,ChinaProjects(BK20200710,BK2018077)supported by the Natural Science Foundation of Jiangsu Province,China+1 种基金Project(NHKY-2019-19)supported by the Nanjing Polytechnic Institute Start Fund,ChinaProject(202012920026Y)supported by the Innovation and Entrepreneurship Training Program of Jiangsu Province College Students,China。
文摘Gold nanoparticles(GNPs)have been extensively used in nanomedicine and neuroscience owing to their biological inertness,peculiar opto-electronic and physico-chemical features.However,the effect of GNPs shape on the neurophysiological properties of single neuron is still unclear.To tackle this issue,different shape GNPs(nanosphere,nanotriakisoctahedron and nanoflower)were synthesized to investigate the effect of GNPs on the voltage-dependent sodium channel and the action potential(AP)of hippocampal CA1 neurons in mice.The results indicated that GNPs inhibited the amplitudes of voltage-gated sodium current(I_(Na))and led to a hyperpolarizing shift in the voltage-dependence curve of both activation and inactivation of I_(Na).GNPs also increased neuronal excitability and altered some properties of AP.Moreover,most alterations in AP properties were observed in nanoflower GNPs treated CA1 neurons,suggesting that the neurotoxicity of gold nanoparticles is surface roughness-dependent.These results may provide a valuable direction in the clinical application of GNPs.
基金Doctoral Program Foundation of State Education Ministry ofChina (No.20010183016) Natural Science Foundation of JilinProvince (No.20020657).
文摘AP and VP were measured in cucumbers under water sufficiency and water stress. The results indicated that, the AP would be evoked by electrical impulse, for water-stressed cucumber, its amplitude could reached more than 40 mV which was obviously greater than that (about 10-20 mV) of plant under well-watered, and no VP came out. Along with the intensity of light increased, the VP appeared going-up trend, and accompanied by evidently spiking electrical signal, for plant under water stress, the VP increased more clearly, but the change of spiking amplitude of AP (about 3 mV) was rather smaller than that (8-10 mV) of plant under well-watered.
