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Screening of Actinomycetes from Medicinal Plant Rhizosphere Soils for Industrial Enzymes and Antimicrobial Activity
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作者 Lukcha Vililuk Tanaporn Wongkuan +1 位作者 Supalak Yacharone Ekachai Chukeatirote 《Journal of Northeast Agricultural University(English Edition)》 CAS 2018年第3期23-28,共6页
This study aimed to investigate the production of some metabolites (i.e., antibiotics, amylases and cellulases) of terrestrial actinomycetes isolated from medicinal plant rhizosphere soils. Initially, the soil sampl... This study aimed to investigate the production of some metabolites (i.e., antibiotics, amylases and cellulases) of terrestrial actinomycetes isolated from medicinal plant rhizosphere soils. Initially, the soil samples were collected from Camellia sinensis (L) Okuntze., Peuraria mirifca Airy Shaw Suvatabandhua., Ananus comosus Merr., Elephantopus scaber Linn., Orthosiphon grandiforus Bolding., Jatropha multifda Linn. and Senna siamea. To screen and isolate actinomycetes, the soil samples were pretreated by air-drying and subsequent heat incubation. The bacterial isolates exhibiting actinomycetes features were then randomly screened for their production of amylases, cellulases and antibiotics. It was found that 130 isolates (from 136) could produce amylases; 40 (from 107) produced cellulases; and seven (from 45) exhibited antimicrobial activity. The data of this study were preliminary, and yet demonstrated a rich diversity of rhizo-actinomycetes from medicinal plants. Besides, these organisms could be an untapped source for discovering of biotechnologically useful metabolites. 展开更多
关键词 actinomycete AMYLASE antibiotic CELLULOSE rhizosphere soil
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拮抗农业致病菌放线菌G5发酵条件的优化 被引量:2
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作者 刘杨 王勇 +1 位作者 康静芹 张良 《湖北农业科学》 北大核心 2014年第7期1634-1636,1689,共4页
微生物的发酵过程十分复杂,培养基配方的组成以及培养条件的变化都会对菌体的代谢造成很大的影响。为了使抗生素的产量达到最大化,试验运用了Plackett-Burman试验设计、最陡爬坡试验、BoxBehnken试验设计对放线菌(Actinomycetes)G5进行... 微生物的发酵过程十分复杂,培养基配方的组成以及培养条件的变化都会对菌体的代谢造成很大的影响。为了使抗生素的产量达到最大化,试验运用了Plackett-Burman试验设计、最陡爬坡试验、BoxBehnken试验设计对放线菌(Actinomycetes)G5进行了发酵条件优化。利用Plackett-Burman试验设计对影响放线菌G5发酵的诸多相关因素进行评估并筛选出3个主要因素,即碳源含量、培养温度和培养时间;之后根据3个主要因素的作用大小和方向进行了最陡爬坡试验;最后运用Box-Behnken试验设计确定最优值,得出其最优培养条件为淀粉含量21.66 g/L、培养温度27.70℃、培养时间7.75 d。在此条件下,抗生素对苹果轮纹病菌的抑菌圈直径为23.00 mm,与模型预测值基本一致。 展开更多
关键词 放线菌(actinomycetes)G5 Plackett—Burman试验设计 最陡爬坡试验 Box—Behnken试验设计 酵条件优化
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