The abortive behaviour and the cytoplasm quality of cytoplasmic male-sterility(CMS)lines of homocaryon were investigated and the existence of C race of Bipolaris maydiswere discussed from the point of view of genetic ...The abortive behaviour and the cytoplasm quality of cytoplasmic male-sterility(CMS)lines of homocaryon were investigated and the existence of C race of Bipolaris maydiswere discussed from the point of view of genetic breeding,physiology and pathology in this paper.Then the countermeasures to prevent the danger from disease of B. maydis in CMS hybrid pro-duction were proposed.展开更多
common maize synthetic rate（ Photosynthetic characteristics were probed by sweet maize, waxy maize, high starch maize and The results revealed that leaf area index （LAI）, chlorophyll a content, chlorophyll b cont...common maize synthetic rate（ Photosynthetic characteristics were probed by sweet maize, waxy maize, high starch maize and The results revealed that leaf area index （LAI）, chlorophyll a content, chlorophyll b content,photo-PR） showed single peak curve at the whole growth stage. The stages of peak were different according to different varieties. NEAUS4 had the lowest peak and while SIDAN 19 had the highest among all stages. Ratio of chlorophyll a to b was low at seedling stage, reached the peak atjointing stage and then declined. SIDAN 19 had the lower level at the last stages.展开更多
The pollen tube pathway method of transformation has been reported to be successful in most crops, but less successfu in maizc. DNA can be transferred by cutting the stigma following pollination and applying the DNA s...The pollen tube pathway method of transformation has been reported to be successful in most crops, but less successfu in maizc. DNA can be transferred by cutting the stigma following pollination and applying the DNA solution in a suitable period DNA presumably reaches the ovary by flowing down the pollen tube and then integrates into the just fertilized but undivided zygotic cells. To provide the molecular evidence for this procedure, the plasmids pGBIRC carrying a CaMV35S promoter-PPT acetyle transferase （bar） gene-nos terminator genc fusion construct were used. Total 3 276 seeds were produced from the ears trcated with DNA. It was found that 35 scedlings were GUS assay positive, but less intense than that of the positive controls, of which 17 were PCR amplification positive. But, only 13 of the seeds from the plants treated with DNA containing the bar gene were found to be resistant compared with the negative control. Less than 1.07% of progeny seedlings tested cxpressed a herbicide positive reaction and polymerase chain reaction （PCR） with seedling DNA did detect the bar genc. Morphological variation was observed in six plants. We succeed in obtain PPT-resistant maize inbred lines via pollen tube pathway展开更多
文摘The abortive behaviour and the cytoplasm quality of cytoplasmic male-sterility(CMS)lines of homocaryon were investigated and the existence of C race of Bipolaris maydiswere discussed from the point of view of genetic breeding,physiology and pathology in this paper.Then the countermeasures to prevent the danger from disease of B. maydis in CMS hybrid pro-duction were proposed.
文摘common maize synthetic rate（ Photosynthetic characteristics were probed by sweet maize, waxy maize, high starch maize and The results revealed that leaf area index （LAI）, chlorophyll a content, chlorophyll b content,photo-PR） showed single peak curve at the whole growth stage. The stages of peak were different according to different varieties. NEAUS4 had the lowest peak and while SIDAN 19 had the highest among all stages. Ratio of chlorophyll a to b was low at seedling stage, reached the peak atjointing stage and then declined. SIDAN 19 had the lower level at the last stages.
文摘The pollen tube pathway method of transformation has been reported to be successful in most crops, but less successfu in maizc. DNA can be transferred by cutting the stigma following pollination and applying the DNA solution in a suitable period DNA presumably reaches the ovary by flowing down the pollen tube and then integrates into the just fertilized but undivided zygotic cells. To provide the molecular evidence for this procedure, the plasmids pGBIRC carrying a CaMV35S promoter-PPT acetyle transferase （bar） gene-nos terminator genc fusion construct were used. Total 3 276 seeds were produced from the ears trcated with DNA. It was found that 35 scedlings were GUS assay positive, but less intense than that of the positive controls, of which 17 were PCR amplification positive. But, only 13 of the seeds from the plants treated with DNA containing the bar gene were found to be resistant compared with the negative control. Less than 1.07% of progeny seedlings tested cxpressed a herbicide positive reaction and polymerase chain reaction （PCR） with seedling DNA did detect the bar genc. Morphological variation was observed in six plants. We succeed in obtain PPT-resistant maize inbred lines via pollen tube pathway
