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Method to analyze wrinkled membranes with zero shear modulus and equivalent stiffness
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作者 赵冉 魏德敏 孙文波 《Journal of Central South University》 SCIE EI CAS 2011年第5期1700-1708,共9页
To solve the problems of divergence,low accuracy and project application of membrane wrinkling analysis,an analysis method of zero shear modulus and equivalent stiffness was proposed.This method is an improvement to t... To solve the problems of divergence,low accuracy and project application of membrane wrinkling analysis,an analysis method of zero shear modulus and equivalent stiffness was proposed.This method is an improvement to the previous method (Method I) of local coordinate transposition and stiffness equivalence.The new method is derived and the feasibility is theoretically proved.A small-scale membrane structure is analyzed by the two methods,and the results show that the computational efficiency of the new method (Method II) is approximately 23 times that of Method I.When Method II is applied to a large-scale membrane stadium structure,it is found that this new method can quickly make the second principal stress of one way wrinkled elements zero,and make the two principal stresses of two-way wrinkled elements zero as well.It could attain the correct load responses right after the appearance of wrinkled elements,which indicates that Method II can be applied to wrinkling analysis of large-scale membrane structures. 展开更多
关键词 membrane structures finite element method wrinkling analysis shear modulus zero-setting equivalent stiffness
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Numerical and experimental investigation on the formability of stainless steel-copper composites during micro deep drawing
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作者 QI Yan-yang MA Xiao-guang +6 位作者 JIANG Zheng-yi MA Li-nan WANG Zhi-hua ZHOU Cun-long HASAN Mahadi DOBRZAŃSKI Leszek A. ZHAO Jing-wei 《Journal of Central South University》 2025年第4期1237-1251,共15页
In the present study,two-layered stainless steel-copper composites with a thickness of 50μm were initially subjected to annealing at 800,900 and 1000℃for 5 min,respectively,to achieve diverse microstructural feature... In the present study,two-layered stainless steel-copper composites with a thickness of 50μm were initially subjected to annealing at 800,900 and 1000℃for 5 min,respectively,to achieve diverse microstructural features.Then the influence of annealing temperature on the formability of stainless steel-copper composites and the quality of micro composite cups manufactured by micro deep drawing(MDD)were investigated,and the underlying mechanism was analyzed.Three finite element(FE)models,including basic FE model,Voronoi FE model and surface morphological FE model,were developed to analyze the forming performance of stainless steel-copper composites during MDD.The results show that the stainless steel-copper composites annealed at 900℃possess the best plasticity owing to the homogeneous and refined microstructure in both stainless steel and copper matrixes,and the micro composite cup with specimen annealed at 900℃exhibits a uniform wall thickness as well as high surface quality with the fewest wrinkles.The results obtained from the surface morphological FE model considering material inhomogeneity and surface morphology of the composites are the closest to the experimental results compared to the basic and Voronoi FE model.During MDD process,the drawing forces decrease with increasing annealing temperature as a consequence of the strength reduction. 展开更多
关键词 micro deep drawing annealing temperature stainless steel-copper composites FORMABILITY WRINKLING finite element method
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油莎豆块茎油脂积累相关基因CeWRI1的克隆与功能分析 被引量:10
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作者 徐硕 邹智 +4 位作者 肖艳华 张丽 孔华 郭静远 郭安平 《热带作物学报》 CSCD 北大核心 2022年第5期923-929,共7页
起源于非洲的油莎豆是迄今唯一已知在块茎中高水平积累油脂的新型草本油料作物。基于我国当前食用植物油和生物柴油原料供给紧张的局面,挖掘参与油莎豆块茎油脂积累的关键基因具有重要的理论意义和应用价值。WRI1(WRINKLED1)隶属于AP2/... 起源于非洲的油莎豆是迄今唯一已知在块茎中高水平积累油脂的新型草本油料作物。基于我国当前食用植物油和生物柴油原料供给紧张的局面,挖掘参与油莎豆块茎油脂积累的关键基因具有重要的理论意义和应用价值。WRI1(WRINKLED1)隶属于AP2/ERF转录因子家族,是一类被证实在油料种子发育过程中控制碳源由糖向油分配的关键基因。本研究采用RT-PCR技术从油莎豆的块茎中分离到一个WRI1同源基因(CeWRI1),该基因的编码区为1116 bp,预测编码371 AA,其理论分子量为41.58 kDa,等电点为5.76,总平均疏水指数为–0.750,不稳定系数为60.75,细胞核定位,这与其转录调控功能是一致的。与拟南芥WRI1类似,序列分析显示CeWRI1含有2个保守的AP2结构域(PF00847)、1个VYL基序和1个14-3-3/BPM结合基序;相比AP2结构域和N端,其C端序列的变异较大,但包含与蛋白降解相关的PEST基序。qRT-PCR分析显示,CeWRI1在叶片、叶鞘、根、匍匐茎和块茎等主要组织中都有表达,且其在起始期、膨大初期、膨大中期、膨大晚期和成熟期等不同发育时期块茎中呈现先降后升的J型表达趋势,表达丰度最高的为成熟期,最低是膨大中期,这与油脂的积累模式大体一致。在烟草中的功能分析显示,CeWRI1的异源瞬时过表达可显著提高叶片的油脂(甘油三酯)含量,这进一步证实该基因具有油脂调控功能。上述结果表明CeWRI1是调控油莎豆块茎高水平积累油脂的关键基因之一,这不仅为进一步揭示油莎豆块茎油脂积累的调控机制奠定了坚实的基础,也为后期的品种改良提供了宝贵的基因资源。 展开更多
关键词 甘油三酯 油脂积累 wrinkleD1 QRT-PCR 瞬时表达
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紫苏AP2基因家族PfWRI1促进植物种子油脂积累 被引量:2
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作者 冯小燕 王其凤 +5 位作者 岳柯昕 侯福朋 徐华祥 陆俊杏 胡建 张涛 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2024年第8期1161-1172,共12页
AP2转录因子属于AP2/ERF超家族,参与调节植物生长发育的各种生物学过程,以及对生物和非生物胁迫的响应。然而,关于紫苏(Perilla frutescens) AP2转录因子家族的研究未见报道。在本研究中,从紫苏基因组中鉴定到18个AP2家族成员,使用生物... AP2转录因子属于AP2/ERF超家族,参与调节植物生长发育的各种生物学过程,以及对生物和非生物胁迫的响应。然而,关于紫苏(Perilla frutescens) AP2转录因子家族的研究未见报道。在本研究中,从紫苏基因组中鉴定到18个AP2家族成员,使用生物信息学方法分析了基因结构、保守基序和顺式作用元件。WRINKLED1 (WRI1)是许多植物物种中脂质生物合成的关键调节因子,在油脂合成过程中发挥重要调控作用。通过序列比对发现其中1个成员与AtWRI1序列高度同源,含有2个保守的AP2结构域,命名为PfWRI1。结合转录物组数据分析了PfAP2家族基因在紫苏不同组织和种子发育不同阶段的表达水平,结果表明,PfWRI1仅在紫苏种子中高表达,暗示PfWRI1可能与种子的发育过程有关。进而构建植物过表达载体pCAMBIA1303-PfWRI1,通过农杆菌侵染技术转化野生型(Col)以及突变体(wri1-1)拟南芥,分别获得过表达和回补株系。结果表明,相比于Col,PfWRI1的表达导致拟南芥种子含油率提高了8.90%~13.57%,并促进转基因拟南芥种子中油酸(C18:1)和亚油酸(18:2)的积累,减少棕榈酸(C16:0)、花生酸(C20:0)和花生一烯酸(C20:1)的积累。此外,外源PfWRI1基因的表达增加了糖酵解和脂肪酸生物合成相关基因AtPKP-α、AtPKP-β1、AtBCCP2、AtSUS2和AtLIP1的表达。综上所述,PfWRI1可能通过与上述基因互作,增加不饱和脂肪酸含量来促进油脂积累。 展开更多
关键词 紫苏 AP2转录因子家族 wrinkleD1 油脂积累
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Analytical and Experimental Studies on Critical Blank-holder Forces in Cylindrical cup deep drawing 被引量:4
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作者 Lin Zhongqin Sun Chengzhi Chen Guanlong Yu Haiyan 《上海交通大学学报》 EI CAS CSCD 北大核心 2004年第z2期101-107,共7页
Based on the plastic deformation theory and energy method, the critical blank-holder forces (BHF) of wrinkling and fracture in the cylindrical cup deep-drawing are analyzed. The verify experiments were performed and c... Based on the plastic deformation theory and energy method, the critical blank-holder forces (BHF) of wrinkling and fracture in the cylindrical cup deep-drawing are analyzed. The verify experiments were performed and compared with the theory analysis results. The predictions agree well with the experiment results. Thus, the validated presenttheoretical analysis model may be applied to determinate the suitable BHF. 展开更多
关键词 blank-holder force wrinkle LIMIT fracture LIMIT sheet metal forming FORMABILITY window
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CO_(2)-diluted CH_(4)-air premixed spherical flames with microwave-assisted spark ignition
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作者 ZHANG Xin-hua WANG Zhao-wen +5 位作者 WU Hui-min LIU Chao-hui WANG Zhi-hao LI Xiao-jie CHENG Xiao-bei CHEN Jyh-Yuan 《Journal of Central South University》 SCIE EI CAS CSCD 2022年第7期2157-2164,共8页
The performance of microwave-assisted spark ignition(MAI)under exhaust gas recirculation conditions was explored with CO_(2)-diluted CH-air premixed spherical flames in a constant volume combustion chamber.The flame k... The performance of microwave-assisted spark ignition(MAI)under exhaust gas recirculation conditions was explored with CO_(2)-diluted CH-air premixed spherical flames in a constant volume combustion chamber.The flame kernel radius at 5 ms after spark started was selected to evaluate the property of MAI for CO_(2)dilution ratio of 0-20%and equivalence ratio of 0.6-1.4 with 1 kHz microwave pulse repetition frequency under 0.2 MPa ambient pressure.The results showed that the addition of microwave induced some wrinkles on the flame surface and strongly deformed the flame.MAI expanded the limit of CO_(2)dilution ratio to 16%with an equivalence ratio of 0.75,in which case the spark only(SI)failed to ignite the mixture.With the CO_(2)dilution ratio increasing,the wrinkles induced by microwave pulses decreased apparently,and the enhancement value of MAI peaked at 4%CO_(2)dilution ratio.The effect of microwave was considered in two aspects,namely,reaction kinetics and thermal effect,which shows a“trade-off”as CO_(2)dilution ratio rose.With 8%volume of CO_(2)added,the flammable interval(equivalence ratio 0.6-1.2)of mixture in SI mode shrunk,and MAI can maintain a flammable interval consistency with the case that no CO_(2)was added. 展开更多
关键词 microwave spark ignition CO_(2)dilution spherical flame flame surface wrinkles
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Multi-Point Forming Technology for Sheet Metal 被引量:10
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作者 LI Ming-zhe, CAI Zhong-yi, SUI Zhen, YAN Qing-guang (Roll Forging Institute, Jilin University of Technology, Changchun 130025, China) 《厦门大学学报(自然科学版)》 CAS CSCD 北大核心 2002年第S1期17-,共1页
Multi-point forming (MPF) is an advanced manufacturing technology for three-dimensional sheet metal parts. In this paper, the MPF integrated system is described that can form a variety of part shapes without the need ... Multi-point forming (MPF) is an advanced manufacturing technology for three-dimensional sheet metal parts. In this paper, the MPF integrated system is described that can form a variety of part shapes without the need for solid dies, and given only geometry and material information about the desired part. The central component of this system is a pair of matrices of punches, and the desired discrete die surface is constructed by changing the positions of punches though the CAD and control system. The basic MPF process is introduced and the typical application examples show the applicability of the MPF technology. Wrinkle and dimple are the major forming defects in MPF process, numerical simulation is a feasible way to predict forming defects in MPF. In conventional stamping, the mode to form sheet metal with blankholder is an effective way to suppress wrinkling; the same is true in MPF. A MPF press with flexible blankholder was developed, and the forming results indicated the forming stability of this technique. Based on the flexibility of MPF, varying deformation path MPF and sectional MPF were explored that cannot be realized in conventional stamping. By controlling each punch in real-time, a sheet part can be manufactured along a specific forming path. When the path of deformation in MPF is designed properly, forming defects will be avoided completely and lager deformation is achieved. A work piece can be formed section by section though the sectional MPF, and this technique makes it possible to manufacture large size parts in a small MPF press. Some critical experiments were performed that confirmed the validity of two special MPF techniques. 展开更多
关键词 sheet metal multi-point forming flexible forming forming path WRINKLING forming defects
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A simple analytical method for deflation prediction of inflatable structures
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作者 李庆松 卿强 龚景海 《Journal of Central South University》 SCIE EI CAS CSCD 2015年第6期2277-2286,共10页
The static performance of inflatable structures has been well studied and the dynamic deployment simulation has received much attention. However, very few studies focus on its deflation behavior. Although there are se... The static performance of inflatable structures has been well studied and the dynamic deployment simulation has received much attention. However, very few studies focus on its deflation behavior. Although there are several dynamic finite element algorithms that can be applied to the deflation simulation, their computation costs are expensive, especially for large scale structures. In this work, a simple method based on classic thermodynamics and the analytical relationship between air and membrane was proposed to efficiently analyze the air state variables under the condition of ventilation. Combined with failure analysis of static bearing capacity, a fast incremental analytical method was presented to predict both elastic and post wrinkling deflation process of inflatable structures. Comparisons between simplified analysis, dynamic finite element simulation, and a full-scale experimental test are presented and the suitability of this simple method for solving the air state and predicting the deflation behavior of inflatable structures is proved. 展开更多
关键词 inflatable structure DEFLATION WRINKLING SIMULATION control volume method
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