Immature zygotic embryos of Quercus variabilis were as explants to induce somatic embryogenesis. Several factors influencing somatic embryogenesis have been assayed. Somatic embryos can be induced in MS and WPM basal ...Immature zygotic embryos of Quercus variabilis were as explants to induce somatic embryogenesis. Several factors influencing somatic embryogenesis have been assayed. Somatic embryos can be induced in MS and WPM basal medium, but there was more quantity, big size and high induction rate in MS medium. Induction rate was not significant cultured in light and dark condition. Zygotic embryos, collected in middle of July, gave higher rate of somatic embryogenesis than those collected on the earlier or later date. By adding 6-BA in medium individually, somatic embryogenesis appeared directly on the zygotic embryos without detectable callus. Secondary embryogenesis appeared in medium with 2,4-D individual or combined with 6-BA or TDZ. High induction frequency of 90% was achieved in MS medium supplemented with 0.5 mg·L -1 6-BA and 2,4-D, whereas the rate in hormone-free medium was only 16.7%. The genotypes of mother trees had an great impact on the inducing rate. Zygotic embryo surgery treatments were not favorable to embryogenesis. It was best to inoculate with entire zygotic embryos. The hypocotyl was a crucial part on somatic embryogenesis for Q. variabilis.展开更多
目的研究正常成人朗读和自发言语的语速特征与差异。方法采集30例正常成人的自发性言语(材料包括"自我介绍"、"我的兴趣爱好"、"我的家乡"等十个话题的自然言语)和朗读《繁星》文段的录像,剪辑表达最稳定...目的研究正常成人朗读和自发言语的语速特征与差异。方法采集30例正常成人的自发性言语(材料包括"自我介绍"、"我的兴趣爱好"、"我的家乡"等十个话题的自然言语)和朗读《繁星》文段的录像,剪辑表达最稳定,停顿、重复、延长发生最少的一分钟录像,对男、女受试者在两种表达方式下的每分钟音节个数(syllables per minute,SPM)和每分钟词语个数(words per minutem,WPM)进行统计分析。结果 1自发言语时SPM(男)=226.13±25.97个、SPM(女)=198.73±40.10个、WPM(男)=119.33±19.90个、WPM(女)=108.00±22.61个;朗读时SPM(男)=208.93.±29.35个、SPM(女)=220.53.±26.52个、WPM(男)=104.67±13.43个、WPM(女)=111.80±12.73个;2自发言语时SPM和WPM之间的Pearson相关性系数r=0.926,朗读时SPM和WPM之间的Pearson相关性系数r=0.969;3独立情况下,性别和表达方式对语速没有显著影响(P=0.42,P=0.72);但性别和表达方式对语速的交互效应显著(P=0.006),女性朗读时语速显著高于其自发言语语速(P=0.025),男性自发言语语速显著高于女性(P=0.035)。结论 SPM和WPM具有极显著相关性;性别和表达方式不是影响语速的重要因素,但性别和表达方式对语速的交互效应显著(P<0.01)。展开更多
目的探讨学龄脑瘫儿童朗读和自发性言语状态下的语速特征。方法采集15例7～15岁学龄脑瘫儿童(脑瘫组)和15例正常儿童(正常组)在自发言语(看图说话)和朗读(朗读"休假"文段)言语状态下的语音样本进行剪辑,剪辑表达最稳定、停顿...目的探讨学龄脑瘫儿童朗读和自发性言语状态下的语速特征。方法采集15例7～15岁学龄脑瘫儿童(脑瘫组)和15例正常儿童(正常组)在自发言语(看图说话)和朗读(朗读"休假"文段)言语状态下的语音样本进行剪辑,剪辑表达最稳定、停顿、重复、延长最少的一分钟音频,对两组受试者在两种表达方式下的每分钟音节个数(syllable per minute,SPM)和每分钟词语个数(words per minute,WPM)进行比较。结果自发言语和朗读两种言语表达状态下,正常组和脑瘫组儿童SPM(正常组分别为141.27±31.69、220.91±35.28个,脑瘫组分别为131.18±32.06、119.63±34.53个)和WPM(正常组分别为86.91±14.63、110.36±17.77个,脑瘫组分别为71.19±18.82、60.45±17.01个)均有极其显著相关性(P<0.001);儿童类型和表达方式是影响语速的重要因素,且儿童类型和表达方式对语速的交互效应极显著(P<0.001)。结论 SPM和WPM具有极显著相关性,儿童类型和表达方式均是影响学龄脑瘫儿童语速的重要因素,且儿童类型和表达方式对语速的交互效应显著。展开更多
文摘Immature zygotic embryos of Quercus variabilis were as explants to induce somatic embryogenesis. Several factors influencing somatic embryogenesis have been assayed. Somatic embryos can be induced in MS and WPM basal medium, but there was more quantity, big size and high induction rate in MS medium. Induction rate was not significant cultured in light and dark condition. Zygotic embryos, collected in middle of July, gave higher rate of somatic embryogenesis than those collected on the earlier or later date. By adding 6-BA in medium individually, somatic embryogenesis appeared directly on the zygotic embryos without detectable callus. Secondary embryogenesis appeared in medium with 2,4-D individual or combined with 6-BA or TDZ. High induction frequency of 90% was achieved in MS medium supplemented with 0.5 mg·L -1 6-BA and 2,4-D, whereas the rate in hormone-free medium was only 16.7%. The genotypes of mother trees had an great impact on the inducing rate. Zygotic embryo surgery treatments were not favorable to embryogenesis. It was best to inoculate with entire zygotic embryos. The hypocotyl was a crucial part on somatic embryogenesis for Q. variabilis.
文摘目的研究正常成人朗读和自发言语的语速特征与差异。方法采集30例正常成人的自发性言语(材料包括"自我介绍"、"我的兴趣爱好"、"我的家乡"等十个话题的自然言语)和朗读《繁星》文段的录像,剪辑表达最稳定,停顿、重复、延长发生最少的一分钟录像,对男、女受试者在两种表达方式下的每分钟音节个数(syllables per minute,SPM)和每分钟词语个数(words per minutem,WPM)进行统计分析。结果 1自发言语时SPM(男)=226.13±25.97个、SPM(女)=198.73±40.10个、WPM(男)=119.33±19.90个、WPM(女)=108.00±22.61个;朗读时SPM(男)=208.93.±29.35个、SPM(女)=220.53.±26.52个、WPM(男)=104.67±13.43个、WPM(女)=111.80±12.73个;2自发言语时SPM和WPM之间的Pearson相关性系数r=0.926,朗读时SPM和WPM之间的Pearson相关性系数r=0.969;3独立情况下,性别和表达方式对语速没有显著影响(P=0.42,P=0.72);但性别和表达方式对语速的交互效应显著(P=0.006),女性朗读时语速显著高于其自发言语语速(P=0.025),男性自发言语语速显著高于女性(P=0.035)。结论 SPM和WPM具有极显著相关性;性别和表达方式不是影响语速的重要因素,但性别和表达方式对语速的交互效应显著(P<0.01)。
文摘目的探讨学龄脑瘫儿童朗读和自发性言语状态下的语速特征。方法采集15例7～15岁学龄脑瘫儿童(脑瘫组)和15例正常儿童(正常组)在自发言语(看图说话)和朗读(朗读"休假"文段)言语状态下的语音样本进行剪辑,剪辑表达最稳定、停顿、重复、延长最少的一分钟音频,对两组受试者在两种表达方式下的每分钟音节个数(syllable per minute,SPM)和每分钟词语个数(words per minute,WPM)进行比较。结果自发言语和朗读两种言语表达状态下,正常组和脑瘫组儿童SPM(正常组分别为141.27±31.69、220.91±35.28个,脑瘫组分别为131.18±32.06、119.63±34.53个)和WPM(正常组分别为86.91±14.63、110.36±17.77个,脑瘫组分别为71.19±18.82、60.45±17.01个)均有极其显著相关性(P<0.001);儿童类型和表达方式是影响语速的重要因素,且儿童类型和表达方式对语速的交互效应极显著(P<0.001)。结论 SPM和WPM具有极显著相关性,儿童类型和表达方式均是影响学龄脑瘫儿童语速的重要因素,且儿童类型和表达方式对语速的交互效应显著。
