The technology of induced pluripotent stem cell(iPSCs)has enabled the conversion of somatic cells into primitive undifferentiated cells via reprogramming.This approach provides possibilities for cell replacement thera...The technology of induced pluripotent stem cell(iPSCs)has enabled the conversion of somatic cells into primitive undifferentiated cells via reprogramming.This approach provides possibilities for cell replacement therapies and drug screening,but the potential risk of tumorigenesis hampers further development and application.How to generate required differentia-ted cells without initiating tumor progression remains a huge challenge.Here we show that mouse embryonic fibroblasts could be differentiated into valvular endothelial cell(VEC)like cells.VECs are critical in valve replacements in aortic valve failure.VEC-associated gene and protein expression and functional assays were quantified for these VEC-like cells.We show that mouse embryonic fibroblasts could be converted into VEC-like cells.Our results suggest that it is possible to convert mouse embryonic fibroblasts into VEC-like cells without first reprogramming them into pluripotent stem cells,minimizing the possibility of tumorigenesis.展开更多
基金supported by funds from Huazhong University of Science and Technology
文摘The technology of induced pluripotent stem cell(iPSCs)has enabled the conversion of somatic cells into primitive undifferentiated cells via reprogramming.This approach provides possibilities for cell replacement therapies and drug screening,but the potential risk of tumorigenesis hampers further development and application.How to generate required differentia-ted cells without initiating tumor progression remains a huge challenge.Here we show that mouse embryonic fibroblasts could be differentiated into valvular endothelial cell(VEC)like cells.VECs are critical in valve replacements in aortic valve failure.VEC-associated gene and protein expression and functional assays were quantified for these VEC-like cells.We show that mouse embryonic fibroblasts could be converted into VEC-like cells.Our results suggest that it is possible to convert mouse embryonic fibroblasts into VEC-like cells without first reprogramming them into pluripotent stem cells,minimizing the possibility of tumorigenesis.
