An abrupt decline in respiratory function often presents in patients unable to undergo imaging procedures,especially in critical care settings.Consequently,evaluation using transthoracic lung ultrasonography has been ...An abrupt decline in respiratory function often presents in patients unable to undergo imaging procedures,especially in critical care settings.Consequently,evaluation using transthoracic lung ultrasonography has been developed to promptly diagnose the patient's respiratory conditions at bedside,gaining increasing attention for its utility.However,conventional transthoracic ultrasonography may face challenges in directly accessing the thorax.展开更多
Objective To evaluate the outcome of off-pump coronary artery bypass grafting （OPCABG） using a bilateral internal mammary artery （BIMA） Y configuration graft to achieve total arterial myocardial revascularization....Objective To evaluate the outcome of off-pump coronary artery bypass grafting （OPCABG） using a bilateral internal mammary artery （BIMA） Y configuration graft to achieve total arterial myocardial revascularization. Methods From October 2002 to December 2008, 208 patients （196 males and 12 females） underwent OPCABG using a BIMA Y configuration graft. The average age of the patients was 56.5 ± 11.3 years, with an age range of 33-78 years. A total of 167 （80.2%） cases had triple-vessel disease. Left main stem disease was found in 33 （15.9%） cases, and double-vessel disease was found in 8 （3.9%） cases. The semi-skeletonization technique was used to harvest the two internal mammary arteries （IMAs）, and then the free right internal mammary artery was connected end-to-side to the left internal mammary artery （LIMA） in situ to complete the Y configuration graft. Off-pump and sequential anastomosis methods were used to perform coronary artery bypass surgery in all patients. Graft patency was assessed intm-operatively with the HT311 transit time flowmeter. Results A total of 728 distal anastomoses were performed in 208 patients, with the average being 3.5± 1.3 per person. No one died or experienced recurrent angina within 30 days after the operation. Conclusions OPCABG using the BIMA Y graft was safe and effective to achieve total arterial revascularization. This method avoids surgical operation on the ascending aorta and other incisions.展开更多
Objective:To compare the reliability of transvaginal ultrasonography with pelvic arteriography in the assessment of patients with gestational trophoblastic disease. Methods. Transvaginal ultrasonography was performed ...Objective:To compare the reliability of transvaginal ultrasonography with pelvic arteriography in the assessment of patients with gestational trophoblastic disease. Methods. Transvaginal ultrasonography was performed in 24 patients with gestational trophoblastic tumour. Within one week after ultrasound investigation, pelvic arteriography was carried out in each patient. Of 24 cases, 16 patients hadn’t been treated by chemical reagent, 5 had accepted 2 to 5 courses of chemotherapy, and 3 had achieved complete remission before both investigations performed. Results. In 3 patients with complete remission, 2 had no evidence of abnormal findings either on transvaginal ultrasonography or on pelvic arteriography, 1 showed intramyometrial lesions by both methods. In the remaining 21 patients, all demostrated a abnormal uterine image, and 5 of them accompanied with the finding of parametrium metastatic signs by transvaginal ultrasonography; these abnormal results were confirmed by pelvic arteriographic imaging. However, in two cases without clinical and ultrasonic signs of parametrium metastasis, pelvic arteriography indicated the early metastasis of parametrium ves- sels. Conclusions. Even though it is difficult to predict the early parametrium metastasis in patients with gestational trophoblastic disease by B-ultrasonic investigation, our data would support the introduction of transvaginal ultrasonography in the diagnosis and evaluation of gestational trophoblastic tumour.展开更多
Objective To explore the contrast-enhanced ultrasonographic features for quantitative assessment of hepatic fibrosis. Methods 86 patients with chronic viral hepatitis B were enrolled in this study from March 2007 to A...Objective To explore the contrast-enhanced ultrasonographic features for quantitative assessment of hepatic fibrosis. Methods 86 patients with chronic viral hepatitis B were enrolled in this study from March 2007 to August 2009. The patients were classified into 5 groups (S0-S4) according to fibrosis stage evaluated with ultrasound guided liver biopsy. New contrast-enhanced ultrasonography (CEUS) features including area under the time-intensity curve (TIC) of portal venous phase/hepatic arterial phase (Qp/Qa) and intensity of portal venous phase/hepatic arterial phase (Ip/Ia) were used to detect the blood supply ratio (portal vein/hepatic artery) in each group. Arrival time of portal vein trunk (Tp) and decreasing rate of TIC (β ) were also analyzed. Results Qp/Qa and Ip/Ia decreased from S0 to S4, while Tp and β increased. These 4 features were significantly correlated with the degree of fibrosis (P<0.001) and were significantly different among the five groups (P<0.001). Sensitivity and specificity of Ip/Ia were 80% and 86% for groups ≥S1, 75% and 86% for groups ≥ S2, 71% and 84% for groups ≥ S3, and 76% and 80% for group S4, respectively. Sensitivity and specificity of Qp/Qa were 70% and 88% for groups ≥ S1, 80% and 76% for groups ≥ S2, 74% and 70% for groups ≥ S3, and 81% and 95% for group S4, respectively. Conclusion Ip/Ia and Qp/Qa could be adopted as reliable, non-invasive features for quantitative assessment of hepatic fibrosis.展开更多
BACKGROUND: Deep venous thrombosis(DVT) is a major cause of morbidity and is a common presenting complaint to the emergency department(ED). Point-of-care two-point compression ultrasonography has evolved as a quick an...BACKGROUND: Deep venous thrombosis(DVT) is a major cause of morbidity and is a common presenting complaint to the emergency department(ED). Point-of-care two-point compression ultrasonography has evolved as a quick and effective way of diagnosing DVT. The purpose of this study is to validate the prevalence and distribution of venous thrombi isolated to proximal lower extremity veins, other than common femoral and popliteal veins in patients with DVT.METHODS: This is a single-center retrospective study that looked at patients presenting to the ED of a tertiary care hospital between January 2014 and August 2018. The clinical presentation and laboratory and imaging results were obtained using the hospital's electronic medical record.RESULTS: A total of 2,507 patients underwent a lower extremity duplex ultrasound during the study period. Among them, 379(15%) were included in the study. The percentages of isolated thrombi to the femoral vein and deep femoral vein were 7.92% and 0.53%, respectively. When the patients were stratified into the two groups of isolated DVT and two-point compression DVT, there were no statistically significant differences in the laboratory results between both groups. However, immobilized patients and patients with recent surgeries were more likely to have an isolated DVT.CONCLUSIONS: Thrombi isolated to proximal lower extremity veins other than the common femoral and popliteal veins make up 8.45% of DVTs. Given this significant number of missed DVTs, the authors recommend the addition of the femoral and deep femoral veins to the two-point compression exam.展开更多
Objective To evaluate color Doppler ultrasonography (CDU) appearances of renal vein thrombosis (RVT) and its diagnostic value.Methods Ten patients with RVT were analyzed retrospectively. Renal structure, distributions...Objective To evaluate color Doppler ultrasonography (CDU) appearances of renal vein thrombosis (RVT) and its diagnostic value.Methods Ten patients with RVT were analyzed retrospectively. Renal structure, distributions of intrarenal flow signals, echogenicity, and flow fullness in main renal veins were observed with CDU. Resistance index (RI) was recorded from the waveforms of segmental or interlobar renal artery.Results Ten kidneys in nine patients were confirmed to have thrombus within the main renal veins, and one patient was confirmed to have thrombus within the small intrarenal veins. The appearances of the main renal vein thrombosis included full of solid echogenicity or strip echogenicity and complete or partial filling defect within the main renal veins, and absent or a few intrarenal venous flow signals in 70% of kidneys involved. The appearances of intrarenal vein thrombosis included obscure renal structure and no venous flow signal within the involved part of the kidneys. Reverse diastolic flow in the intrarenal artery had only a sensitivity of 36% (4/11); in other 7 kidneys without intrarenal arterial reverse diastolic flow, increased RI (mean, 0.84; range, 0.74-0.96) was found.Conclusion CDU is helpful for rapid clinical diagnosis and follow-up of RVT, and therefore can be the first imaging modality of choice for RVT.展开更多
The scintiphotos and ultrasonophotos of 186 patients with focal and diffuse diseases of liver were collected and analysed. All the data were get from the in-patients of China-Japan Friendship Hospital. The Sigma 438 t...The scintiphotos and ultrasonophotos of 186 patients with focal and diffuse diseases of liver were collected and analysed. All the data were get from the in-patients of China-Japan Friendship Hospital. The Sigma 438 typedγ camera (USA) was used. Fifteen min after 99mTc-phytate were injected i.v., If any defect region was found, it was recorded as a SOL; the SSA 90A, SAL 77A and SAL 50A US scanners (Japan) were used, if any abnormal echo pattern was found, it was a SOL. Of 134 patients have done both examinations during 7 days, 122 patients had coincident results occupied 91.0%. For detection of SOL, the sensitivity of scintigraphy is 94.1%, specificity is 94.2%, accuracy is 94.8%; the sensitivity of US is 100%, specificity is 89.7%, the accuracy is 95.6%. The hepatic blood flow and blood pool study showed that 104 from 115 patients were coincident (90.4%). The spleen and liver accumulation ratio determination showed that 51 from 64 subjects were coincident (80.0%). The characteristic features of scintigraphy and US graphy were described in hepatoma, hemangioma, cyst abscess and cirrhosis. The advantages of these examinations were discussed and concluded that their procedures are valuable and widely available.展开更多
Sixty-one intermediate and advanced esophageal carcinoma patients were examined by transesophageal ultrasonography(TEUS),including 53 squamous carcinomas and 8 adenocarcinomas. The ultrasonic images showed that there ...Sixty-one intermediate and advanced esophageal carcinoma patients were examined by transesophageal ultrasonography(TEUS),including 53 squamous carcinomas and 8 adenocarcinomas. The ultrasonic images showed that there were clearly demarcated spaces between the tumours and the important adjacent structures in 42 out of 48 patients having undergone esophagectomy,while in 10 non-resectable patients,the ultrasonic images displayed an unclear demarcation between the tumours and thoracic aorta,pulmonary vein and pericardium. The study discovered that the regional echo of thoracic aortic wall was interrupted and replaced by tumour hypoecho, and the aorta pressed,indicating carcinoma's serious invasion of the aorta. The non-resectability of carcinoma of this kind was later confirmed in operation. On 3 cases,operations couldn't be performed due to metastasis of liver.Six cases failed to be accurately assessed owing to the impassability for probe caused by esophageal stenosis or other factors. Accuracy of assessment was 85. 2%. So we believe TEUS is a useful,simple,reliable and secure method for assessing operability of esophageal carcinoma.展开更多
Dear editor,Pancreatic carcinoma is highly malignant and is mainly characterized by a low rate of eligibility for curative-intent resection,rapid metastases and/or local relapses even after surgery.[1-3]Imaging examin...Dear editor,Pancreatic carcinoma is highly malignant and is mainly characterized by a low rate of eligibility for curative-intent resection,rapid metastases and/or local relapses even after surgery.[1-3]Imaging examinations like enhanced computed tomography(CT)/magnetic resonance imaging(MRI),and positron emission tomography-computed tomography(PET-CT),play an important role in detecting tumor recurrence or residual after radical resection of pancreatic cancer.However,these detection platforms are increasingly reported to have insufficient sensitivity and specificity.[4,5]Here we report a pancreatic cancer patient who had progressively increased carbohydrate antigen 19-9(CA199)level one year and three months after surgery;however,none of the above imaging methods were able to identify the cause.After our investigations,we discovered mediastinal metastasis using endoscopic ultrasonography(EUS)and confi rmed by EUS-guided fi ne needle aspiration(EUS-FNA).展开更多
A 55-year-old man was admitted for transcatheter closure because of pulmonary arteriovenous fistula by magnetic resonance angiography （MRA）. He had a history of occasional chest pain more than one year. Angiography ...A 55-year-old man was admitted for transcatheter closure because of pulmonary arteriovenous fistula by magnetic resonance angiography （MRA）. He had a history of occasional chest pain more than one year. Angiography didn＇t reveal significant stenosis at coronary artery. The patient was found a continuous grade 2/6 murmur over the left upper parastenal area one month ago. Chest MRA revealed a possible left superior pulmonary arteriovenous fistula. For diagnosis and localization of the fistula, aortography and selective angiography of the intemal mammary artery was performed and presented a left internal mammary artery-to-pulmonary artery fistula. The fistula was successfully closed using an 12 mm domestic vascular plug. Chest MRA showed that the fistula disappeared at two-month follow-up展开更多
Mammary epithelial cells with lactational function can be a valuable cellular model for research of the development and regulation of the mammary gland.This paper describes some aspects of function of an epithelial ce...Mammary epithelial cells with lactational function can be a valuable cellular model for research of the development and regulation of the mammary gland.This paper describes some aspects of function of an epithelial cell line from the mammary gland of the dairy goat.SDS-PAGE,triglyceride and lactose content of cultured cells were used to assess synthetic function of cells and the effects of exposure to insulin and prolactin.Results show that goat mammary epithelial cells can synthesize fat,proteins and lactose when they were cultured in DMEM-F12 medium with added EGF,IGF-1,ITS and FBS.There were no obvious changes after 48h treatment with additional insulin.Prolactin added to the basal medium significantly increased synthesis of proteins and lactose.A mammary gland epithelial cell line from goats which has lactational function has been established.This outcome provides a valuable and convenient model system.展开更多
The aim of this study was to investigate the effect of duodenal infusion of soybean small peptides(SSP) on mammary uptake of amino acids(AA).Eight Chinese Saanen goats with duodenal fistulae and catheters were used in...The aim of this study was to investigate the effect of duodenal infusion of soybean small peptides(SSP) on mammary uptake of amino acids(AA).Eight Chinese Saanen goats with duodenal fistulae and catheters were used in a crossover design trial.Goats were infused with 0,60,120,180 g/d SSP.The experimental period lasted for 14 d.The results showed:1) milk protein yield and content were increased,with the increment in milk protein yield being significant(P【0.05).Milk fat yield and content were decreased with the increased amount of SSP infused(P【0.05).2) Mammary plasma flow was not changed(P】0.05) by SSP infusion though the average was slightly higher.Mammary plasma flow/milk yield was decreased by SSP infusion and there was significant difference between the 120 g/d treatment and the control(P【0.05).3) Compared with the control treatment,uptakes of most free amino acids were increased in the 60 and 120 g/d treatments,but decreased by the 180 g/d treatment.The uptakes of all peptide-bound essential amino acids(PB-EAA) were increased except for PB-Ile.Uptake of PB-Val,PB-Leu, PB-Phe,PB-Thr,PB-Met and PB-Lys was highest in the 120 g/d treatment.Among the peptide-bound nonessential amino acids(PB-NEAA),uptake of PB-Ser,PB-Tyr,and PB-Pro was increased(P【0.05) while that of PB-Gly was decreased(P】0.05).4) With the exception of Lys,secretion of all essential AA(EAA) was increased by SSP infusion from 0-120 g/d(P【0.05),while in the 180 g/d treatment the increase was not significant and was lower than that in the 120 g/d treatment.5) The expression of APN was increased by SSP;in the 60,120,180 g/d treatments expression was 13.55-,18.69-,and 10.01-fold over that of the controls.Conclusion:SSP might be used as substrates for milk protein synthesis by mammary gland,and can increase synthesis and secretion of milk protein.There was reason to believe that uptake and use of AA was either saturated or inhibited at high levels of infusion of SSP.Increased APN expression with infusion of SSP related to mammary uptake of PBAA by mammary gland,which suggested that APN was one of the peptidases regulating use of AA from small peptides in mammary tissue.展开更多
Objective： To evaluate the inhibitory effect of heparanase antisense oligodeoxynucleotide （AS-ODN） on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice. Methods： The AS-ODN co...Objective： To evaluate the inhibitory effect of heparanase antisense oligodeoxynucleotide （AS-ODN） on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice. Methods： The AS-ODN complementary to the start codon region of heparanase mRNA and its control, scrambled nonsense oligodeoxynucleotide （NS-ODN） were designed and synthesized. A subcutaneous growth model and an acute hematogenous metastasis model of human mammary carcinoma were established in nude mice and were treated with ODNs. The heparanase expression in tumor was evaluated by RT-PCR and Western blot. The microvessel density （MVD） was measured by immunohistochemistry for factor VS. The tumor volume was calculated and lung metastatic nodules were counted. Results ： The heparanase expression, MVD, tumor volume and lung metastatic nodules in AS-ODN treated group were significantly decreased compared with that in NS-ODN treated group and that in PBS group （P〈0.01）. Conclusion ： Heparanase AS-ODN has significant inhibitory effect on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice.展开更多
Mammary abscess,also termed acute mastitis,is an acute suppurative disease characterized by breast lumps,redness,swelling,heat,and pain. It is commonly seen in primiparous lactating women 3-4 weeks after parturition. ...Mammary abscess,also termed acute mastitis,is an acute suppurative disease characterized by breast lumps,redness,swelling,heat,and pain. It is commonly seen in primiparous lactating women 3-4 weeks after parturition. It is believed that the occurrence of acute mastitis is closely associated with stress,a surplus of nutrients during pregnancy,overreliance on breast pumps and lack of breastfeeding experience. In recent years,traditional Chinese medicine( TCM)has been extensively applied in the treatment of acute mastitis because of its low cost and high safety,and has been considered to be the first-choice treatment of acute mastitis in China. Therefore,this article mainly explored the key points of common syndromes,TCM nursing methods and health guidance of acute mastitis in order to further develop the advantages of TCM,improve its efficacy and standardized its nursing behavior.展开更多
文摘An abrupt decline in respiratory function often presents in patients unable to undergo imaging procedures,especially in critical care settings.Consequently,evaluation using transthoracic lung ultrasonography has been developed to promptly diagnose the patient's respiratory conditions at bedside,gaining increasing attention for its utility.However,conventional transthoracic ultrasonography may face challenges in directly accessing the thorax.
文摘Objective To evaluate the outcome of off-pump coronary artery bypass grafting （OPCABG） using a bilateral internal mammary artery （BIMA） Y configuration graft to achieve total arterial myocardial revascularization. Methods From October 2002 to December 2008, 208 patients （196 males and 12 females） underwent OPCABG using a BIMA Y configuration graft. The average age of the patients was 56.5 ± 11.3 years, with an age range of 33-78 years. A total of 167 （80.2%） cases had triple-vessel disease. Left main stem disease was found in 33 （15.9%） cases, and double-vessel disease was found in 8 （3.9%） cases. The semi-skeletonization technique was used to harvest the two internal mammary arteries （IMAs）, and then the free right internal mammary artery was connected end-to-side to the left internal mammary artery （LIMA） in situ to complete the Y configuration graft. Off-pump and sequential anastomosis methods were used to perform coronary artery bypass surgery in all patients. Graft patency was assessed intm-operatively with the HT311 transit time flowmeter. Results A total of 728 distal anastomoses were performed in 208 patients, with the average being 3.5± 1.3 per person. No one died or experienced recurrent angina within 30 days after the operation. Conclusions OPCABG using the BIMA Y graft was safe and effective to achieve total arterial revascularization. This method avoids surgical operation on the ascending aorta and other incisions.
文摘Objective:To compare the reliability of transvaginal ultrasonography with pelvic arteriography in the assessment of patients with gestational trophoblastic disease. Methods. Transvaginal ultrasonography was performed in 24 patients with gestational trophoblastic tumour. Within one week after ultrasound investigation, pelvic arteriography was carried out in each patient. Of 24 cases, 16 patients hadn’t been treated by chemical reagent, 5 had accepted 2 to 5 courses of chemotherapy, and 3 had achieved complete remission before both investigations performed. Results. In 3 patients with complete remission, 2 had no evidence of abnormal findings either on transvaginal ultrasonography or on pelvic arteriography, 1 showed intramyometrial lesions by both methods. In the remaining 21 patients, all demostrated a abnormal uterine image, and 5 of them accompanied with the finding of parametrium metastatic signs by transvaginal ultrasonography; these abnormal results were confirmed by pelvic arteriographic imaging. However, in two cases without clinical and ultrasonic signs of parametrium metastasis, pelvic arteriography indicated the early metastasis of parametrium ves- sels. Conclusions. Even though it is difficult to predict the early parametrium metastasis in patients with gestational trophoblastic disease by B-ultrasonic investigation, our data would support the introduction of transvaginal ultrasonography in the diagnosis and evaluation of gestational trophoblastic tumour.
基金Supported by PhD Programs Foundation of Ministry of Education of China (No. 20090001110092)
文摘Objective To explore the contrast-enhanced ultrasonographic features for quantitative assessment of hepatic fibrosis. Methods 86 patients with chronic viral hepatitis B were enrolled in this study from March 2007 to August 2009. The patients were classified into 5 groups (S0-S4) according to fibrosis stage evaluated with ultrasound guided liver biopsy. New contrast-enhanced ultrasonography (CEUS) features including area under the time-intensity curve (TIC) of portal venous phase/hepatic arterial phase (Qp/Qa) and intensity of portal venous phase/hepatic arterial phase (Ip/Ia) were used to detect the blood supply ratio (portal vein/hepatic artery) in each group. Arrival time of portal vein trunk (Tp) and decreasing rate of TIC (β ) were also analyzed. Results Qp/Qa and Ip/Ia decreased from S0 to S4, while Tp and β increased. These 4 features were significantly correlated with the degree of fibrosis (P<0.001) and were significantly different among the five groups (P<0.001). Sensitivity and specificity of Ip/Ia were 80% and 86% for groups ≥S1, 75% and 86% for groups ≥ S2, 71% and 84% for groups ≥ S3, and 76% and 80% for group S4, respectively. Sensitivity and specificity of Qp/Qa were 70% and 88% for groups ≥ S1, 80% and 76% for groups ≥ S2, 74% and 70% for groups ≥ S3, and 81% and 95% for group S4, respectively. Conclusion Ip/Ia and Qp/Qa could be adopted as reliable, non-invasive features for quantitative assessment of hepatic fibrosis.
文摘BACKGROUND: Deep venous thrombosis(DVT) is a major cause of morbidity and is a common presenting complaint to the emergency department(ED). Point-of-care two-point compression ultrasonography has evolved as a quick and effective way of diagnosing DVT. The purpose of this study is to validate the prevalence and distribution of venous thrombi isolated to proximal lower extremity veins, other than common femoral and popliteal veins in patients with DVT.METHODS: This is a single-center retrospective study that looked at patients presenting to the ED of a tertiary care hospital between January 2014 and August 2018. The clinical presentation and laboratory and imaging results were obtained using the hospital's electronic medical record.RESULTS: A total of 2,507 patients underwent a lower extremity duplex ultrasound during the study period. Among them, 379(15%) were included in the study. The percentages of isolated thrombi to the femoral vein and deep femoral vein were 7.92% and 0.53%, respectively. When the patients were stratified into the two groups of isolated DVT and two-point compression DVT, there were no statistically significant differences in the laboratory results between both groups. However, immobilized patients and patients with recent surgeries were more likely to have an isolated DVT.CONCLUSIONS: Thrombi isolated to proximal lower extremity veins other than the common femoral and popliteal veins make up 8.45% of DVTs. Given this significant number of missed DVTs, the authors recommend the addition of the femoral and deep femoral veins to the two-point compression exam.
文摘Objective To evaluate color Doppler ultrasonography (CDU) appearances of renal vein thrombosis (RVT) and its diagnostic value.Methods Ten patients with RVT were analyzed retrospectively. Renal structure, distributions of intrarenal flow signals, echogenicity, and flow fullness in main renal veins were observed with CDU. Resistance index (RI) was recorded from the waveforms of segmental or interlobar renal artery.Results Ten kidneys in nine patients were confirmed to have thrombus within the main renal veins, and one patient was confirmed to have thrombus within the small intrarenal veins. The appearances of the main renal vein thrombosis included full of solid echogenicity or strip echogenicity and complete or partial filling defect within the main renal veins, and absent or a few intrarenal venous flow signals in 70% of kidneys involved. The appearances of intrarenal vein thrombosis included obscure renal structure and no venous flow signal within the involved part of the kidneys. Reverse diastolic flow in the intrarenal artery had only a sensitivity of 36% (4/11); in other 7 kidneys without intrarenal arterial reverse diastolic flow, increased RI (mean, 0.84; range, 0.74-0.96) was found.Conclusion CDU is helpful for rapid clinical diagnosis and follow-up of RVT, and therefore can be the first imaging modality of choice for RVT.
基金The Project was supported by the International Atomic Energy Agency
文摘The scintiphotos and ultrasonophotos of 186 patients with focal and diffuse diseases of liver were collected and analysed. All the data were get from the in-patients of China-Japan Friendship Hospital. The Sigma 438 typedγ camera (USA) was used. Fifteen min after 99mTc-phytate were injected i.v., If any defect region was found, it was recorded as a SOL; the SSA 90A, SAL 77A and SAL 50A US scanners (Japan) were used, if any abnormal echo pattern was found, it was a SOL. Of 134 patients have done both examinations during 7 days, 122 patients had coincident results occupied 91.0%. For detection of SOL, the sensitivity of scintigraphy is 94.1%, specificity is 94.2%, accuracy is 94.8%; the sensitivity of US is 100%, specificity is 89.7%, the accuracy is 95.6%. The hepatic blood flow and blood pool study showed that 104 from 115 patients were coincident (90.4%). The spleen and liver accumulation ratio determination showed that 51 from 64 subjects were coincident (80.0%). The characteristic features of scintigraphy and US graphy were described in hepatoma, hemangioma, cyst abscess and cirrhosis. The advantages of these examinations were discussed and concluded that their procedures are valuable and widely available.
文摘Sixty-one intermediate and advanced esophageal carcinoma patients were examined by transesophageal ultrasonography(TEUS),including 53 squamous carcinomas and 8 adenocarcinomas. The ultrasonic images showed that there were clearly demarcated spaces between the tumours and the important adjacent structures in 42 out of 48 patients having undergone esophagectomy,while in 10 non-resectable patients,the ultrasonic images displayed an unclear demarcation between the tumours and thoracic aorta,pulmonary vein and pericardium. The study discovered that the regional echo of thoracic aortic wall was interrupted and replaced by tumour hypoecho, and the aorta pressed,indicating carcinoma's serious invasion of the aorta. The non-resectability of carcinoma of this kind was later confirmed in operation. On 3 cases,operations couldn't be performed due to metastasis of liver.Six cases failed to be accurately assessed owing to the impassability for probe caused by esophageal stenosis or other factors. Accuracy of assessment was 85. 2%. So we believe TEUS is a useful,simple,reliable and secure method for assessing operability of esophageal carcinoma.
基金Group Medical Aid Project of the Tibet Autonomous Region Natural Science Foundation(XZ2020ZR-ZY28[Z]).
文摘Dear editor,Pancreatic carcinoma is highly malignant and is mainly characterized by a low rate of eligibility for curative-intent resection,rapid metastases and/or local relapses even after surgery.[1-3]Imaging examinations like enhanced computed tomography(CT)/magnetic resonance imaging(MRI),and positron emission tomography-computed tomography(PET-CT),play an important role in detecting tumor recurrence or residual after radical resection of pancreatic cancer.However,these detection platforms are increasingly reported to have insufficient sensitivity and specificity.[4,5]Here we report a pancreatic cancer patient who had progressively increased carbohydrate antigen 19-9(CA199)level one year and three months after surgery;however,none of the above imaging methods were able to identify the cause.After our investigations,we discovered mediastinal metastasis using endoscopic ultrasonography(EUS)and confi rmed by EUS-guided fi ne needle aspiration(EUS-FNA).
文摘A 55-year-old man was admitted for transcatheter closure because of pulmonary arteriovenous fistula by magnetic resonance angiography （MRA）. He had a history of occasional chest pain more than one year. Angiography didn＇t reveal significant stenosis at coronary artery. The patient was found a continuous grade 2/6 murmur over the left upper parastenal area one month ago. Chest MRA revealed a possible left superior pulmonary arteriovenous fistula. For diagnosis and localization of the fistula, aortography and selective angiography of the intemal mammary artery was performed and presented a left internal mammary artery-to-pulmonary artery fistula. The fistula was successfully closed using an 12 mm domestic vascular plug. Chest MRA showed that the fistula disappeared at two-month follow-up
基金supported by Innovation Team Project of Northeast Agricultural Vniversity(XLT005-1-2)Research Fund for the Doctoral Program of Heilongjiang Educational Committee(HLJBSDJI2004-15)
文摘Mammary epithelial cells with lactational function can be a valuable cellular model for research of the development and regulation of the mammary gland.This paper describes some aspects of function of an epithelial cell line from the mammary gland of the dairy goat.SDS-PAGE,triglyceride and lactose content of cultured cells were used to assess synthetic function of cells and the effects of exposure to insulin and prolactin.Results show that goat mammary epithelial cells can synthesize fat,proteins and lactose when they were cultured in DMEM-F12 medium with added EGF,IGF-1,ITS and FBS.There were no obvious changes after 48h treatment with additional insulin.Prolactin added to the basal medium significantly increased synthesis of proteins and lactose.A mammary gland epithelial cell line from goats which has lactational function has been established.This outcome provides a valuable and convenient model system.
文摘The aim of this study was to investigate the effect of duodenal infusion of soybean small peptides(SSP) on mammary uptake of amino acids(AA).Eight Chinese Saanen goats with duodenal fistulae and catheters were used in a crossover design trial.Goats were infused with 0,60,120,180 g/d SSP.The experimental period lasted for 14 d.The results showed:1) milk protein yield and content were increased,with the increment in milk protein yield being significant(P【0.05).Milk fat yield and content were decreased with the increased amount of SSP infused(P【0.05).2) Mammary plasma flow was not changed(P】0.05) by SSP infusion though the average was slightly higher.Mammary plasma flow/milk yield was decreased by SSP infusion and there was significant difference between the 120 g/d treatment and the control(P【0.05).3) Compared with the control treatment,uptakes of most free amino acids were increased in the 60 and 120 g/d treatments,but decreased by the 180 g/d treatment.The uptakes of all peptide-bound essential amino acids(PB-EAA) were increased except for PB-Ile.Uptake of PB-Val,PB-Leu, PB-Phe,PB-Thr,PB-Met and PB-Lys was highest in the 120 g/d treatment.Among the peptide-bound nonessential amino acids(PB-NEAA),uptake of PB-Ser,PB-Tyr,and PB-Pro was increased(P【0.05) while that of PB-Gly was decreased(P】0.05).4) With the exception of Lys,secretion of all essential AA(EAA) was increased by SSP infusion from 0-120 g/d(P【0.05),while in the 180 g/d treatment the increase was not significant and was lower than that in the 120 g/d treatment.5) The expression of APN was increased by SSP;in the 60,120,180 g/d treatments expression was 13.55-,18.69-,and 10.01-fold over that of the controls.Conclusion:SSP might be used as substrates for milk protein synthesis by mammary gland,and can increase synthesis and secretion of milk protein.There was reason to believe that uptake and use of AA was either saturated or inhibited at high levels of infusion of SSP.Increased APN expression with infusion of SSP related to mammary uptake of PBAA by mammary gland,which suggested that APN was one of the peptidases regulating use of AA from small peptides in mammary tissue.
文摘Objective： To evaluate the inhibitory effect of heparanase antisense oligodeoxynucleotide （AS-ODN） on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice. Methods： The AS-ODN complementary to the start codon region of heparanase mRNA and its control, scrambled nonsense oligodeoxynucleotide （NS-ODN） were designed and synthesized. A subcutaneous growth model and an acute hematogenous metastasis model of human mammary carcinoma were established in nude mice and were treated with ODNs. The heparanase expression in tumor was evaluated by RT-PCR and Western blot. The microvessel density （MVD） was measured by immunohistochemistry for factor VS. The tumor volume was calculated and lung metastatic nodules were counted. Results ： The heparanase expression, MVD, tumor volume and lung metastatic nodules in AS-ODN treated group were significantly decreased compared with that in NS-ODN treated group and that in PBS group （P〈0.01）. Conclusion ： Heparanase AS-ODN has significant inhibitory effect on the angiogenesis and metastasis of human mammary carcinoma cell xenografts in nude mice.
文摘Mammary abscess,also termed acute mastitis,is an acute suppurative disease characterized by breast lumps,redness,swelling,heat,and pain. It is commonly seen in primiparous lactating women 3-4 weeks after parturition. It is believed that the occurrence of acute mastitis is closely associated with stress,a surplus of nutrients during pregnancy,overreliance on breast pumps and lack of breastfeeding experience. In recent years,traditional Chinese medicine( TCM)has been extensively applied in the treatment of acute mastitis because of its low cost and high safety,and has been considered to be the first-choice treatment of acute mastitis in China. Therefore,this article mainly explored the key points of common syndromes,TCM nursing methods and health guidance of acute mastitis in order to further develop the advantages of TCM,improve its efficacy and standardized its nursing behavior.
