Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect samples.In this paper,we surveyed the prevale...Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect samples.In this paper,we surveyed the prevalence of Wolbachia using a PCR detection method in ten Tenebrionidae stored-product insects.Subsequently,the spatiotemporal Wolbachia infection dynamics in Tribolium confusum and Wolbachia elimination patterns using tetracycline treatment were investigated in detail by TaqMan®probe real-time quantitative PCR,and host reproductive fitness parameters were compared.T.confusum was the only Wolbachia infected species in all the surveyed species.Wolbachia infection density consistently increased with the development of T.confusumand plateaued at 3.7×107 wsp copies per individual insect at the young adult stage.Wolbachia densities in females were higher than that in males with a significant difference at the pupae stage and varied among different tissues and organs.Uninfected female beetles were completely incapable of producing mature progenies when crossed with Wolbachia infected males.Embryogenesis and egg hatch rate were specifically inhibited after Wolbachia elimination,while other traits,including the number of eggs,pupation rate and sex ratio,remained unaffected by tetracycline treatment.Our results showthat the TaqMan®probe qPCR is a reliable detection method for quantifying the density of Wolbachia as compared to qualitative detection of wsp gene by PCR and relatively quantified by real-time qPCR.The fitness results indicated that Wolbachia infection was not an obligate symbiont and benefited the host confused flour beetle.展开更多
In order to elucidate the ability of stored product insects to resist high temperature at varying temperature conditions,the influence of different heating rates on the mortality of all life stages of Tribolium castan...In order to elucidate the ability of stored product insects to resist high temperature at varying temperature conditions,the influence of different heating rates on the mortality of all life stages of Tribolium castaneum(Herbst)(Coleoptera:Tenebrionidae)was investigated.After acclimation from 28 to 42,44 and 46℃ at different heating rates(0,1.25,2.50,5.00,and 10.00℃/h),eggs,larvae,pupae,and adults of T.castaneum were exposed at 50℃ for 0(as a control),10,15,20,25 and 30 min,respectively.The corrected mortality of T.castaneum was determined after 24 h.The results showed that different exposure times and heating rates had significant effects on the mortality of T.castaneum.The mortality of all life stages of T.castaneum with the same acclimation experience increased significantly with the increasing exposure time,and acclimation improved the heat tolerance of T.castaneum.The mortalities exposed at 50℃ for 30 min of T.castaneum eggs(acclimated to 46℃ at 5.00℃/h),larvae(acclimated to 44℃ at 2.50℃/h),pupae(acclimated to 46℃ at 5.00℃/h),and adults(acclimated to 46℃ at 2.50℃/h)were 62.50%,54.44%,58.89%and 53.33%,respectively,significantly lower than that of corresponding life stages of T.castaneum acclimated at 1.25 and 10.00℃/h.The present results are in favor of developing an effective heat treatment protocol for pest control.展开更多
赤拟谷盗(Tribolium castaneum)是一种世界性的储粮害虫,对粮食的安全储藏构成了巨大威胁,对赤拟谷盗研究进行总结以发现更有效的防治方法。本研究利用中国知网与Web of Science数据库,借助文献信息可视化分析软件CiteSpace,对赤拟谷盗...赤拟谷盗(Tribolium castaneum)是一种世界性的储粮害虫,对粮食的安全储藏构成了巨大威胁,对赤拟谷盗研究进行总结以发现更有效的防治方法。本研究利用中国知网与Web of Science数据库,借助文献信息可视化分析软件CiteSpace,对赤拟谷盗现有的文献进行分析,主要综述了2002—2022年间国内外对赤拟谷盗的研究热点和未来发展趋势。关于赤拟谷盗的研究热点主要集中于RNA干扰的应用、植物精油的开发、激素对生长发育的调控、磷化氢抗性。未来其研究趋势主要是绿色杀虫剂的开发应用、揭示其生理功能机制,以及将功能基因组学的方法应用于储粮害虫行为学、生态学和综合防治等方面。通过本文的分析,为探索赤拟谷盗的基础研究和综合治理提供参考和创新思路。展开更多
基金supported by National Natural Science Fund Project (No.31601890)the Fundamental Research Funds for the Henan Provincial Colleges and Universities in Henan University of Technology (No.2016XTCX01)
文摘Insect symbionts Wolbachia used for pest population control has focused on vector pest species and agricultural insects while rare reports in Chinese stored-product insect samples.In this paper,we surveyed the prevalence of Wolbachia using a PCR detection method in ten Tenebrionidae stored-product insects.Subsequently,the spatiotemporal Wolbachia infection dynamics in Tribolium confusum and Wolbachia elimination patterns using tetracycline treatment were investigated in detail by TaqMan®probe real-time quantitative PCR,and host reproductive fitness parameters were compared.T.confusum was the only Wolbachia infected species in all the surveyed species.Wolbachia infection density consistently increased with the development of T.confusumand plateaued at 3.7×107 wsp copies per individual insect at the young adult stage.Wolbachia densities in females were higher than that in males with a significant difference at the pupae stage and varied among different tissues and organs.Uninfected female beetles were completely incapable of producing mature progenies when crossed with Wolbachia infected males.Embryogenesis and egg hatch rate were specifically inhibited after Wolbachia elimination,while other traits,including the number of eggs,pupation rate and sex ratio,remained unaffected by tetracycline treatment.Our results showthat the TaqMan®probe qPCR is a reliable detection method for quantifying the density of Wolbachia as compared to qualitative detection of wsp gene by PCR and relatively quantified by real-time qPCR.The fitness results indicated that Wolbachia infection was not an obligate symbiont and benefited the host confused flour beetle.
基金supported by National Natural Science Foundation of China(No.U1604115)Henan Provincial Key Scientific and Technological R&D Project(No.182102110111)。
文摘In order to elucidate the ability of stored product insects to resist high temperature at varying temperature conditions,the influence of different heating rates on the mortality of all life stages of Tribolium castaneum(Herbst)(Coleoptera:Tenebrionidae)was investigated.After acclimation from 28 to 42,44 and 46℃ at different heating rates(0,1.25,2.50,5.00,and 10.00℃/h),eggs,larvae,pupae,and adults of T.castaneum were exposed at 50℃ for 0(as a control),10,15,20,25 and 30 min,respectively.The corrected mortality of T.castaneum was determined after 24 h.The results showed that different exposure times and heating rates had significant effects on the mortality of T.castaneum.The mortality of all life stages of T.castaneum with the same acclimation experience increased significantly with the increasing exposure time,and acclimation improved the heat tolerance of T.castaneum.The mortalities exposed at 50℃ for 30 min of T.castaneum eggs(acclimated to 46℃ at 5.00℃/h),larvae(acclimated to 44℃ at 2.50℃/h),pupae(acclimated to 46℃ at 5.00℃/h),and adults(acclimated to 46℃ at 2.50℃/h)were 62.50%,54.44%,58.89%and 53.33%,respectively,significantly lower than that of corresponding life stages of T.castaneum acclimated at 1.25 and 10.00℃/h.The present results are in favor of developing an effective heat treatment protocol for pest control.
文摘赤拟谷盗(Tribolium castaneum)是一种世界性的储粮害虫,对粮食的安全储藏构成了巨大威胁,对赤拟谷盗研究进行总结以发现更有效的防治方法。本研究利用中国知网与Web of Science数据库,借助文献信息可视化分析软件CiteSpace,对赤拟谷盗现有的文献进行分析,主要综述了2002—2022年间国内外对赤拟谷盗的研究热点和未来发展趋势。关于赤拟谷盗的研究热点主要集中于RNA干扰的应用、植物精油的开发、激素对生长发育的调控、磷化氢抗性。未来其研究趋势主要是绿色杀虫剂的开发应用、揭示其生理功能机制,以及将功能基因组学的方法应用于储粮害虫行为学、生态学和综合防治等方面。通过本文的分析,为探索赤拟谷盗的基础研究和综合治理提供参考和创新思路。
