Background:Upland cotton(Gossypium hirsutum)accounts for more than 90%of the annual world cotton output because of its high yield potential.However,yield and fiber quality traits often show negative correlations.We co...Background:Upland cotton(Gossypium hirsutum)accounts for more than 90%of the annual world cotton output because of its high yield potential.However,yield and fiber quality traits often show negative correlations.We constructed four F_(2)populations of upland cotton,using two normal lines(4133B and SGK9708)with high yield potential but moderate fiber quality and two introgression lines(Suyuan04–3 and J02–247)with superior fiber quality,and used them to investigate the genetic basis underlying complex traits such as yield and fiber quality in upland cotton.We also phenotyped eight agronomic and economic traits and mapped quantitative trait loci(QTLs).Results:Extensive phenotype variations and transgressive segregation were found across the segregation populations.We constructed four genetic maps of 585.97 centiMorgan(cM),752.45 cM,752.45 cM,and 1163.66 cM,one for each of the four F_(2)populations.Fifty QTLs were identified across the four populations(7 for plant height,27 for fiber quality and 16 for yield).The same QTLs were identified in different populations,including qBW4 and qBW2,which were linked to a common simple sequence repeat(SSR)marker,NAU1255.A QTL cluster containing eight QTLs for six different traits was characterized on linkage group 9 of the 4133B×Suyuan04–3 population.Conclusions:These findings will provide insights into the genetic basis of simultaneous improvement of yield and fiber quality in upland cotton breeding.展开更多
This study aims to determine the differences in leaf functional traits and phenotypic plasticity of leaf functional traits between exotic and native Compositae plant species. Leaf width of exotic plants was significan...This study aims to determine the differences in leaf functional traits and phenotypic plasticity of leaf functional traits between exotic and native Compositae plant species. Leaf width of exotic plants was significantly lower than that of native species. Leaf length, specific leaf area(SLA), single-leaf wet and dry weights, leaf moisture, and leaf thickness of exotic plants were also lower than those of native species but not significantly. The leaf shape index of exotic plants was higher than that of native species but not significantly. This implies that the relatively low leaf construction cost for exotic plants may play an important role in the success of their invasions. The higher leaf shape index and lower leaf width of exotic plants can enhance the efficiency of resource capture(especially sunlight capture) via adjustments to leaf shape and size, thereby increasing the survival of exotic plants. The plasticity indices of single-leaf wet weight and leaf thickness of exotic plants were significantly lower than those of native species. The lower phenotypic plasticity of single-leaf wet weight and leaf thickness of exotic plants may be the result of a cost to plasticity. That is, if the plasticity is too high, the fitness of plant species might be reduced sharply under unfavorable environments. Thus, lower plasticity of leaf functional traits may compensate for the negative impact of adverse environments and stabilize leaf construction costs for exotic plants. Moreover, reduced phenotypic plasticity might be one of the key competitive strategies by which exotic plants successfully invade new habitats. Overall, exotic plants did not always exhibit higher values of leaf functional traits or increased phenotypic plasticity of leaf functional traits compared with native species.展开更多
The uncoupling protein(UCP)is a member of the mitochondrial membrane transporter family,which plays an important role in energy metabolism.In the present study,the UCP gene was considered as a candidate gene for chick...The uncoupling protein(UCP)is a member of the mitochondrial membrane transporter family,which plays an important role in energy metabolism.In the present study,the UCP gene was considered as a candidate gene for chicken growth traits,and the association of UCP gene SNPs with growth rate was investigated in the eighth generation of NEAUHLF broiler lines.Two SNPs were found in chicken UCP gene,and the association analysis results showed that both the individual and combination of chicken UCPgene SNPs were significantly associated with body weight of 7 weeks(BW7)and carcass weight(CW)(P〈0.05),and the combination had much significant effects than the single SNP.This research suggested that the UCP gene could be a candidate gene or linked to a major gene which affected growth traits in chicken.展开更多
Background:Sucking insect pests cause severe damage to cotton crop production.The development of insect resistant cotton cultivars is one of the most effective measures in curtailing the yield losses.Considering the r...Background:Sucking insect pests cause severe damage to cotton crop production.The development of insect resistant cotton cultivars is one of the most effective measures in curtailing the yield losses.Considering the role of morphological and biochemical host plant resista nee(HPR)traits in plant defense,12 cotton genotypes/varieties were evaluated for leaf area,leaf glanding,total soluble sugars,total soluble proteins,total phenolics,tannin and total flavonoids against fluctuating populations of whitefly,thrips and jassid under field conditions.Results:The population of these insects fluctuated during the growing seas on and remained above threshold level(whitefly>5,thrips>(8-10)f or jassid>1 per leaf)during late June and early July.Strong and negative association of whitefly(r=-0.825)and jassid(r=-0.929)with seed cotton yield was observed.Mean population of insects were the highest in Glandless-1 followed by NIA-82 and NIA-M30.NIAB-Kiran followed by NI AB-878 and Sadori were the most resistant,with the mean population of 1.41,1.60,1.66(whitefly);2.24,232,2.53(thrips)and 037,0.31,036(jassid),respectively.The resistant variety NIAB-Kiran showed less soluble sugars(8.54 mg.g^(-1)),soluble proteins(27.11 mg.g^(-1))and more phenolic(36.56 mg.g^(-1))and flavonoids(13.10mg.g^(-1))as compared with the susceptible check Glandless-1.Moreover,all insect populations were positively correlated with total soluble sugars and proteins.Whitefly populations exhibited negative response to leaf gossypol glands,total phenolics,tannins and flavonoids.The thrips and jassid populations had a significant and negative correlation with these four biochemical HPR traits.Conclusion:The ide ntified resistant resources and HPR traits can be deployed against sucking in sect pests'complex in future breeding programs of developing insect resistant cotton varieties.展开更多
Background:Genetic improvement in fiber quality is one of the main challenges for cotton breeders.Quantitative trait loci(QTL)mapping provides a powerful approach to dissect the molecular mechanism in fiber quality tr...Background:Genetic improvement in fiber quality is one of the main challenges for cotton breeders.Quantitative trait loci(QTL)mapping provides a powerful approach to dissect the molecular mechanism in fiber quality traits.In present study,F14 recombinant inbred line(RIL)population was backcrossed to paternal parent for a paternal backcross(BC/P)population,deriving from one upland cotton hybrid.Three repetitive BC/P field trials and one maternal backcross(BC/M)field trial were performed including both two BC populations and the original RIL population.Results:In total,24 novel QTLs are detected for fiber quality traits and among which 13 QTLs validated previous results.Thirty-five QTLs in BC/P populations explain 5.01%–22.09%of phenotype variation(PV).Among the 35 QTLs,23 QTLs are detected in BC/P population alone.Present study provides novel alleles of male parent for fiber quality traits with positive genetic effects.Particularly,qFS-Chr3–1 explains 22.09%of PV in BC/P population,which increaseds 0.48 cN·tex−1 for fiber strength.A total of 7,2,8,2 and 6 QTLs explain over 10.00%of PV for fiber length,fiber uniformity,fiber strength,fiber elongation and fiber micronaire,respectively.In RIL population,six common QTLs are detected in more than one environment:qFL-Chr1–2,qFS-Chr5–1,qFS-Chr9–1,qFS-Chr21–1,qFM-Chr9–1 and qFM-Chr9–2.Two common QTLs of qFE-Chr2–2(TMB2386-SWU12343)and qFM-Chr9–1(NAU2873-CGR6771)explain 22.42%and 21.91%of PV.The region between NAU4034 and TMB1296 harbor 30 genes(379 kb)in A05 and 42 genes(49 kb)in D05 for fiber length along the QTL qFL-Chr5–1 in BC/P population,respectively.In addition,a total of 142 and 46 epistatic QTLs and QTL×environments(E-QTLs and QQEs)are identified in recombinant inbred lines in paternal backcross(RIL-P)and paternal backcross(BC/P)populations,respectively.Conclusions:The present studies provide informative basis for improving cotton fiber quality in different populations.展开更多
Background: Cotton fiber yield is a complex trait,which can be influenced by multiple agronomic traits.Unravelling the genetic basis of cotton fiber yield-related traits contributes to genetic improvement of cotton.Re...Background: Cotton fiber yield is a complex trait,which can be influenced by multiple agronomic traits.Unravelling the genetic basis of cotton fiber yield-related traits contributes to genetic improvement of cotton.Results: In this study,503 upland cotton varieties covering the four breeding stages(BS1–BS4,1911–2011)in China were used for association mapping and domestication analysis.One hundred and forty SSR markers significantly associated with ten fiber yield-related traits were identified,among which,29 markers showed an increasing trend contribution to cotton yield-related traits from BS1 to BS4,and 26 markers showed decreased trend effect.Four favorable alleles of 9 major loci(R^(2)≥3)were strongly selected during the breeding stages,and the candidate genes of the four strongly selected alleles were predicated according to the gene function annotation and tissue expression data.Conclusions :The study not only uncovers the genetic basis of 10 cotton yield-related traits but also provides genetic evidence for cotton improvement during the cotton breeding process in China.展开更多
Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) c...Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.展开更多
Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,b...Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,both cotton yield and quality should be enhanced.However,cotton yield展开更多
Both the stoichiometric characteristics and leaf functional traits can reflect the adaptability of plants to changes in the external living environment.In particular,for varying aged forest plants,the study of stoichi...Both the stoichiometric characteristics and leaf functional traits can reflect the adaptability of plants to changes in the external living environment.In particular,for varying aged forest plants,the study of stoichiometric characteristics and leaf functional traits can reflect the plant’s life history strategy and its resource investment and allocation methods.In this paper,the 1.5-year-old,5-year-old,15-year-old and 20-year-old Castanopsis hystrix were selected as the research objects to investigate the trends of stoichiometry and leaf functional traits,and their synergistic changes were verified.The results showed that with the increase of age,the stability of C.hystrix stoichiometry gradually increased,and the functional traits also tended to invest in security.However,there was no synergistic change between the two,which might be attributed to the different sensitivity of the C.hystrix leaf to the environment at the life history level of 1.5-year-old to 20-year-old.展开更多
Background:Meta-analysis of quantitative trait locus(QTL)is a computational technique to identify consensus QTL and refine QTL positions on the consensus map from multiple mapping studies.The combination of meta-QTL i...Background:Meta-analysis of quantitative trait locus(QTL)is a computational technique to identify consensus QTL and refine QTL positions on the consensus map from multiple mapping studies.The combination of meta-QTL intervals,significant SNPs and transcriptome analysis has been widely used to identify candidate genes in various plants.Results:In our study,884 QTLs associated with cotton fiber quality traits from 12 studies were used for meta-QTL analysis based on reference genome TM-1,as a result,74 meta-QTLs were identified,including 19 meta-QTLs for fiber length;18 meta-QTLs for fiber strength;11 meta-QTLs for fiber uniformity;11 meta-QTLs for fiber elongation;and 15 meta-QTLs for micronaire.Combined with 8589 significant single nucleotide polymorphisms associated with fiber quality traits collected from 15 studies,297 candidate genes were identified in the meta-QTL intervals,20 of which showed high expression levels specifically in the developing fibers.According to the function annotations,some of the 20 key candidate genes are associated with the fiber development.Conclusions:This study provides not only stable QTLs used for marker-assisted selection,but also candidate genes to uncover the molecular mechanisms for cotton fiber development.展开更多
Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were construc...Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were constructed from the single traits,using factor analysis.The coding region of melanocortin receptor-4(MC4R)gene was amplified by PCR and the products were digested with four restriction endonucleases Hinf I,Ear I,Pst I,and Pvu II to detect restriction fragment length polymorphism(RFLP)in the MC4R gene,to analyze its associations with the traits.The only RFLP was exposed by digesting with Hinf I indicating two genotypes,HH and Hh,with frequencies of allele H and h being 0.8902 and 0.1098 in the F_1 population.Significant effects of genotype HH were found on carcass weight,eviscerated weight(with giblet),liver weight,heart weight,stomach percentage,abdominal fat percentage,breast muscle drip water loss and down proportion and many of the compound indices,and all genotypic contribution percentages(CP,genotypic effect on the population mean)were between-5%and 5%;effects of genotype Hh with the MC4R gene mutation,were not significant.Effects of genotype Hh were significant on two of the compound indices related to fat deposition:the fat index(CP=3.314%,P<0.05)and fatty acid quality(CP=1.218%,P<0.05).展开更多
The reproductive,adaptive heterosis andeconomically-valuable traits of hybrids F<sub>1</sub>,F<sub>2</sub>and F<sub>3</sub> obtained as a result intraspecific crossingG.hirsutum L.p...The reproductive,adaptive heterosis andeconomically-valuable traits of hybrids F<sub>1</sub>,F<sub>2</sub>and F<sub>3</sub> obtained as a result intraspecific crossingG.hirsutum L.parents with ordinary type ofleaves and imported Okra-leaves parents hadbeen studied.Perspective of use of cotton展开更多
Molecular map construction is very important togene marking,map-based cloning and marker-assisted breeding.Some advance was made inthis area in the US(Shappley et al,1996;1998;Reinisch et al,1994),but little work was ...Molecular map construction is very important togene marking,map-based cloning and marker-assisted breeding.Some advance was made inthis area in the US(Shappley et al,1996;1998;Reinisch et al,1994),but little work was donein China.A preliminary molecular map withRFLP,RAPD and SSR was constructed andQTL maping was dong for some agronomic traitsby our research group.展开更多
Molecular markers provide the opportunity toidentify marker-quantitative trait locus(QTL)associations in different environments andpopulations,and can be used to improve theefficiency of conventional plant breeding by...Molecular markers provide the opportunity toidentify marker-quantitative trait locus(QTL)associations in different environments andpopulations,and can be used to improve theefficiency of conventional plant breeding bycarrying out indirect selection through molecularmarkers linked to the interest traits of QTL展开更多
Bone morphogenetic protein-2 （BMP-2） plays a key role in bone formation and maintenance. BMP-2 can stimulate longitudinal bone growth by increasing the growth plate chondrocyte proliferation, hypertrophy, and cartil...Bone morphogenetic protein-2 （BMP-2） plays a key role in bone formation and maintenance. BMP-2 can stimulate longitudinal bone growth by increasing the growth plate chondrocyte proliferation, hypertrophy, and cartilage matrix synthesis. The current study was designed to investigate the associations of the BMP-2 gene polymorphism with chicken skeletal traits. Northeast Agricultural University F2 resource population was used in this study. Body weight and body composition traits were measured in F2 population. Polymorphism between parental lines was detected by DNA sequencing, and PCR-fragment length polymorphism method was then developed to screen the population. The results showed that the BMP-2 gene polymorphism was associated with skeletal traits in F2 population. This research suggests that BMP-2 gene may be a candidate locus or linked to a major gene that affects skeletal traits in chickens.展开更多
The study aims to analyze the distribution of the 313 bp indel （insertion/deletion termed as indel） in first intron of POU1F1 and it＇s association with reproduction traits in Sutai pigs by using the PCR-DSCP techni...The study aims to analyze the distribution of the 313 bp indel （insertion/deletion termed as indel） in first intron of POU1F1 and it＇s association with reproduction traits in Sutai pigs by using the PCR-DSCP technique. The results showed that in this commercial pig population, the frequency of allele A was 0.6371, B was 0.3629; the genotype frequency of AA was 0.4516, AB was 0.3710, BB was 0.1774, and the Z2 test showed that the allele frequencies were in Hardy-Weinberg equilibrium. The SPSS GLM procedure was used to identify the association of the 313 bp indel with reproductive traits. In Sutai pigs, the pigs with AA genotype represented higher value in all reproduction traits, except for higher survival rate of piglets at weaning. Higher weaning weight was significantly associated with AA genotype pigs and higher survival rate of piglets at weaning was significantly associated with BB genotype （P〈0.05）, but no significant differences in other reproduction traits among genotypes were found （P〉0.05）; the P value of different traits affected by fixed factors were not significant as well （P〉0.05）. The result indicated that although this 313 bp indel was significantly associated with the weaning weight and survival rate at weaning, no any association with major reproduction traits was observed in Sutai pigs.展开更多
Correction:J Cotton Res 7,20(2024)https://doi.org/10.1186/s42397-024-00180-3 Following publication of the original article(Shui et al.2024),the author found 5 errors in the published article.1.One of the author’s nam...Correction:J Cotton Res 7,20(2024)https://doi.org/10.1186/s42397-024-00180-3 Following publication of the original article(Shui et al.2024),the author found 5 errors in the published article.1.One of the author’s name has been corrected from Gou Chunping to Guo Chunping.2.The reference(Zhao SQ.2016)in Table 2 has been updated to:Zhao SQ.Analysis on the major gene and multigene mixed inheritance and QTL mapping for early maturity traits in upland cotton.Chin Acad Agric Sci.2016.https://doi.org/10.3969/j.issn.201600501.(in Chinese with English abstract).3.In’Results’part,’Phenotype analysis of 238 cotton boll abscission among cotton accessions’paragraph,the phenotype analysis of AR1 ranging from 19.27%–63.79%,which was wrongly written as 19.27%-63.97%.4.The‘2018KRL’is modified to‘2018KEL’in Table 1.展开更多
1.Development of EST-SSRs derived from G.barbadense:One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G.barbadense ...1.Development of EST-SSRs derived from G.barbadense:One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G.barbadense cv.3-79.Among the SSRs,trinucleotide AAG appeared展开更多
Improvement of seed yield of soybean(Glycine max(L.)Merr.)is generally achieved by combining morphological and yield-related traits,such as plant height(PH),node number on main stem(NN),pod number per plant(NP),seed n...Improvement of seed yield of soybean(Glycine max(L.)Merr.)is generally achieved by combining morphological and yield-related traits,such as plant height(PH),node number on main stem(NN),pod number per plant(NP),seed number per plant(NS),100-seed weight(HSW)and seed weight per plant(SWPP).Identifying quantitative trait loci(QTLs)for morphological and yield-related traits is therefore important for breeding.In this study,a four-way recombinant inbred line population comprising 160 lines derived from the cross(Kenfeng14×Kenfeng15)×(Heinong48×Kenfeng19)was planted in five different environments and morphological and yield-related trait data were used to identify QTLs by the inclusive composite interval mapping method.Totally 38 QTLs for PH,40 QTLs for NN,26 QTLs for NP,10 QTLs for NS,26 QTLs for HSW and 49 QTLs for SWPP were detected in 125 genomic regions.Single QTLs explained 2.17%-14.60%,2.00%-10.04%,2.37%-9.77%,2.62%-8.61%,0.47%-6.51%and 0.14%-12.39%of the phenotypic variation for PH,NN,NP,NS,HSW and SWPP,respectively.Among these 125 genomic regions,120 were newly associated with morphological and yield-related traits.The results would facilitate the molecular breeding of morphological and yield-related traits in soybean.展开更多
Cotton(Gossypium spp.) is the leading fiber crop,and an important source of the important edible oil and protein meals in the world.Complex genetics and strong environmental effects hinder
基金the National Key R&D Program of China(2017YFD0101600).
文摘Background:Upland cotton(Gossypium hirsutum)accounts for more than 90%of the annual world cotton output because of its high yield potential.However,yield and fiber quality traits often show negative correlations.We constructed four F_(2)populations of upland cotton,using two normal lines(4133B and SGK9708)with high yield potential but moderate fiber quality and two introgression lines(Suyuan04–3 and J02–247)with superior fiber quality,and used them to investigate the genetic basis underlying complex traits such as yield and fiber quality in upland cotton.We also phenotyped eight agronomic and economic traits and mapped quantitative trait loci(QTLs).Results:Extensive phenotype variations and transgressive segregation were found across the segregation populations.We constructed four genetic maps of 585.97 centiMorgan(cM),752.45 cM,752.45 cM,and 1163.66 cM,one for each of the four F_(2)populations.Fifty QTLs were identified across the four populations(7 for plant height,27 for fiber quality and 16 for yield).The same QTLs were identified in different populations,including qBW4 and qBW2,which were linked to a common simple sequence repeat(SSR)marker,NAU1255.A QTL cluster containing eight QTLs for six different traits was characterized on linkage group 9 of the 4133B×Suyuan04–3 population.Conclusions:These findings will provide insights into the genetic basis of simultaneous improvement of yield and fiber quality in upland cotton breeding.
基金Project(31300343)supported by the National Natural Science Foundation of ChinaProject(Y20160023)supported by Open Science Research Fund of State Key Laboratory of Soil and Sustainable Agriculture,Institute of Soil Science,Chinese Academy of Sciences,Chinasupported by Jiangsu Collaborative Innovation Center of Technology and Material of Water Treatment,China
文摘This study aims to determine the differences in leaf functional traits and phenotypic plasticity of leaf functional traits between exotic and native Compositae plant species. Leaf width of exotic plants was significantly lower than that of native species. Leaf length, specific leaf area(SLA), single-leaf wet and dry weights, leaf moisture, and leaf thickness of exotic plants were also lower than those of native species but not significantly. The leaf shape index of exotic plants was higher than that of native species but not significantly. This implies that the relatively low leaf construction cost for exotic plants may play an important role in the success of their invasions. The higher leaf shape index and lower leaf width of exotic plants can enhance the efficiency of resource capture(especially sunlight capture) via adjustments to leaf shape and size, thereby increasing the survival of exotic plants. The plasticity indices of single-leaf wet weight and leaf thickness of exotic plants were significantly lower than those of native species. The lower phenotypic plasticity of single-leaf wet weight and leaf thickness of exotic plants may be the result of a cost to plasticity. That is, if the plasticity is too high, the fitness of plant species might be reduced sharply under unfavorable environments. Thus, lower plasticity of leaf functional traits may compensate for the negative impact of adverse environments and stabilize leaf construction costs for exotic plants. Moreover, reduced phenotypic plasticity might be one of the key competitive strategies by which exotic plants successfully invade new habitats. Overall, exotic plants did not always exhibit higher values of leaf functional traits or increased phenotypic plasticity of leaf functional traits compared with native species.
基金Supported by the Earmarked Fund for Modern Agro-industry Technology Research System(CARS-42)Program for Innovation Research Team in University of Heilongjiang Province(2010td02)
文摘The uncoupling protein(UCP)is a member of the mitochondrial membrane transporter family,which plays an important role in energy metabolism.In the present study,the UCP gene was considered as a candidate gene for chicken growth traits,and the association of UCP gene SNPs with growth rate was investigated in the eighth generation of NEAUHLF broiler lines.Two SNPs were found in chicken UCP gene,and the association analysis results showed that both the individual and combination of chicken UCPgene SNPs were significantly associated with body weight of 7 weeks(BW7)and carcass weight(CW)(P〈0.05),and the combination had much significant effects than the single SNP.This research suggested that the UCP gene could be a candidate gene or linked to a major gene which affected growth traits in chicken.
文摘Background:Sucking insect pests cause severe damage to cotton crop production.The development of insect resistant cotton cultivars is one of the most effective measures in curtailing the yield losses.Considering the role of morphological and biochemical host plant resista nee(HPR)traits in plant defense,12 cotton genotypes/varieties were evaluated for leaf area,leaf glanding,total soluble sugars,total soluble proteins,total phenolics,tannin and total flavonoids against fluctuating populations of whitefly,thrips and jassid under field conditions.Results:The population of these insects fluctuated during the growing seas on and remained above threshold level(whitefly>5,thrips>(8-10)f or jassid>1 per leaf)during late June and early July.Strong and negative association of whitefly(r=-0.825)and jassid(r=-0.929)with seed cotton yield was observed.Mean population of insects were the highest in Glandless-1 followed by NIA-82 and NIA-M30.NIAB-Kiran followed by NI AB-878 and Sadori were the most resistant,with the mean population of 1.41,1.60,1.66(whitefly);2.24,232,2.53(thrips)and 037,0.31,036(jassid),respectively.The resistant variety NIAB-Kiran showed less soluble sugars(8.54 mg.g^(-1)),soluble proteins(27.11 mg.g^(-1))and more phenolic(36.56 mg.g^(-1))and flavonoids(13.10mg.g^(-1))as compared with the susceptible check Glandless-1.Moreover,all insect populations were positively correlated with total soluble sugars and proteins.Whitefly populations exhibited negative response to leaf gossypol glands,total phenolics,tannins and flavonoids.The thrips and jassid populations had a significant and negative correlation with these four biochemical HPR traits.Conclusion:The ide ntified resistant resources and HPR traits can be deployed against sucking in sect pests'complex in future breeding programs of developing insect resistant cotton varieties.
基金the National Key R&D Program for Crop Breeding(2016YFD0101407)to Hua JP.
文摘Background:Genetic improvement in fiber quality is one of the main challenges for cotton breeders.Quantitative trait loci(QTL)mapping provides a powerful approach to dissect the molecular mechanism in fiber quality traits.In present study,F14 recombinant inbred line(RIL)population was backcrossed to paternal parent for a paternal backcross(BC/P)population,deriving from one upland cotton hybrid.Three repetitive BC/P field trials and one maternal backcross(BC/M)field trial were performed including both two BC populations and the original RIL population.Results:In total,24 novel QTLs are detected for fiber quality traits and among which 13 QTLs validated previous results.Thirty-five QTLs in BC/P populations explain 5.01%–22.09%of phenotype variation(PV).Among the 35 QTLs,23 QTLs are detected in BC/P population alone.Present study provides novel alleles of male parent for fiber quality traits with positive genetic effects.Particularly,qFS-Chr3–1 explains 22.09%of PV in BC/P population,which increaseds 0.48 cN·tex−1 for fiber strength.A total of 7,2,8,2 and 6 QTLs explain over 10.00%of PV for fiber length,fiber uniformity,fiber strength,fiber elongation and fiber micronaire,respectively.In RIL population,six common QTLs are detected in more than one environment:qFL-Chr1–2,qFS-Chr5–1,qFS-Chr9–1,qFS-Chr21–1,qFM-Chr9–1 and qFM-Chr9–2.Two common QTLs of qFE-Chr2–2(TMB2386-SWU12343)and qFM-Chr9–1(NAU2873-CGR6771)explain 22.42%and 21.91%of PV.The region between NAU4034 and TMB1296 harbor 30 genes(379 kb)in A05 and 42 genes(49 kb)in D05 for fiber length along the QTL qFL-Chr5–1 in BC/P population,respectively.In addition,a total of 142 and 46 epistatic QTLs and QTL×environments(E-QTLs and QQEs)are identified in recombinant inbred lines in paternal backcross(RIL-P)and paternal backcross(BC/P)populations,respectively.Conclusions:The present studies provide informative basis for improving cotton fiber quality in different populations.
基金This work was supported by the National Natural Science Foundation of China(31760402)Young and Middle-aged Science and Technology Leading Talents of Xinjiang Production and Construction Corps(2019CB027).
文摘Background: Cotton fiber yield is a complex trait,which can be influenced by multiple agronomic traits.Unravelling the genetic basis of cotton fiber yield-related traits contributes to genetic improvement of cotton.Results: In this study,503 upland cotton varieties covering the four breeding stages(BS1–BS4,1911–2011)in China were used for association mapping and domestication analysis.One hundred and forty SSR markers significantly associated with ten fiber yield-related traits were identified,among which,29 markers showed an increasing trend contribution to cotton yield-related traits from BS1 to BS4,and 26 markers showed decreased trend effect.Four favorable alleles of 9 major loci(R^(2)≥3)were strongly selected during the breeding stages,and the candidate genes of the four strongly selected alleles were predicated according to the gene function annotation and tissue expression data.Conclusions :The study not only uncovers the genetic basis of 10 cotton yield-related traits but also provides genetic evidence for cotton improvement during the cotton breeding process in China.
文摘Blood samples were taken from birds,from a commercial line of broiler chicken,being in a fully fed state and in fasting state for 2,4,6 hours respectively at 49 d of age and plasma very low density lipoprotein(VLDL) concentration was measured with turbidimetric assay.The experiment results showed that the variation coefficients of plasma VLDL concentration mersured at four different times were 58.11%,74.39%,69.70%,47.83%,respectively for male broilers,and 41.82%,60.87%.59.26%,50.0%for female broilers respectively.Plasma VLDL concentration decreased with the fasting time.There was a significant positive phenotypic correlation between abdominal fat weight,percentage of abdominal fat and plasma VLDL concentration for the birds being in fully feeding state,and the correlation decreased or no longer existed for the bired being in fasting state.The conclusion was that for practically application of plasma VLDL content selected in broiler chicken breeding,blood samples should be collected from birds being in feeding state.
文摘Cotton is one of the most important economic crops in the world,and it provides natural fiber for the textile industry.With the advancement of the textile technology and increased consumption demands on cotton fiber,both cotton yield and quality should be enhanced.However,cotton yield
基金Provincial Science and Technology Research Project of Guangdong(2014KJcx022).
文摘Both the stoichiometric characteristics and leaf functional traits can reflect the adaptability of plants to changes in the external living environment.In particular,for varying aged forest plants,the study of stoichiometric characteristics and leaf functional traits can reflect the plant’s life history strategy and its resource investment and allocation methods.In this paper,the 1.5-year-old,5-year-old,15-year-old and 20-year-old Castanopsis hystrix were selected as the research objects to investigate the trends of stoichiometry and leaf functional traits,and their synergistic changes were verified.The results showed that with the increase of age,the stability of C.hystrix stoichiometry gradually increased,and the functional traits also tended to invest in security.However,there was no synergistic change between the two,which might be attributed to the different sensitivity of the C.hystrix leaf to the environment at the life history level of 1.5-year-old to 20-year-old.
基金This work was supported by the National Natural Science Foundation of China(31760402)Public Welfare Research Projects in the Autonomous Region(KY2019002)Special Programs for New Varieties Cultivation of Shihezi University(YZZX201701).
文摘Background:Meta-analysis of quantitative trait locus(QTL)is a computational technique to identify consensus QTL and refine QTL positions on the consensus map from multiple mapping studies.The combination of meta-QTL intervals,significant SNPs and transcriptome analysis has been widely used to identify candidate genes in various plants.Results:In our study,884 QTLs associated with cotton fiber quality traits from 12 studies were used for meta-QTL analysis based on reference genome TM-1,as a result,74 meta-QTLs were identified,including 19 meta-QTLs for fiber length;18 meta-QTLs for fiber strength;11 meta-QTLs for fiber uniformity;11 meta-QTLs for fiber elongation;and 15 meta-QTLs for micronaire.Combined with 8589 significant single nucleotide polymorphisms associated with fiber quality traits collected from 15 studies,297 candidate genes were identified in the meta-QTL intervals,20 of which showed high expression levels specifically in the developing fibers.According to the function annotations,some of the 20 key candidate genes are associated with the fiber development.Conclusions:This study provides not only stable QTLs used for marker-assisted selection,but also candidate genes to uncover the molecular mechanisms for cotton fiber development.
基金supported by a loth 5-year personwith-ability prject of Anhui province,Natural Science Foundation of Anhui Educational Institution(No.2006KJ/69B)Key Scientific Project of Anhui Provincial Department of Science and Technology(No.07020303055,08010302070)
文摘Carcass,meat quality,and feather-down traits of 123 geese from an F_1 population of Wanxi White goose(male parent)and Sichuan White goose(female parent)were measured,and an additional 12 compound indices were constructed from the single traits,using factor analysis.The coding region of melanocortin receptor-4(MC4R)gene was amplified by PCR and the products were digested with four restriction endonucleases Hinf I,Ear I,Pst I,and Pvu II to detect restriction fragment length polymorphism(RFLP)in the MC4R gene,to analyze its associations with the traits.The only RFLP was exposed by digesting with Hinf I indicating two genotypes,HH and Hh,with frequencies of allele H and h being 0.8902 and 0.1098 in the F_1 population.Significant effects of genotype HH were found on carcass weight,eviscerated weight(with giblet),liver weight,heart weight,stomach percentage,abdominal fat percentage,breast muscle drip water loss and down proportion and many of the compound indices,and all genotypic contribution percentages(CP,genotypic effect on the population mean)were between-5%and 5%;effects of genotype Hh with the MC4R gene mutation,were not significant.Effects of genotype Hh were significant on two of the compound indices related to fat deposition:the fat index(CP=3.314%,P<0.05)and fatty acid quality(CP=1.218%,P<0.05).
文摘The reproductive,adaptive heterosis andeconomically-valuable traits of hybrids F<sub>1</sub>,F<sub>2</sub>and F<sub>3</sub> obtained as a result intraspecific crossingG.hirsutum L.parents with ordinary type ofleaves and imported Okra-leaves parents hadbeen studied.Perspective of use of cotton
文摘Molecular map construction is very important togene marking,map-based cloning and marker-assisted breeding.Some advance was made inthis area in the US(Shappley et al,1996;1998;Reinisch et al,1994),but little work was donein China.A preliminary molecular map withRFLP,RAPD and SSR was constructed andQTL maping was dong for some agronomic traitsby our research group.
文摘Molecular markers provide the opportunity toidentify marker-quantitative trait locus(QTL)associations in different environments andpopulations,and can be used to improve theefficiency of conventional plant breeding bycarrying out indirect selection through molecularmarkers linked to the interest traits of QTL
基金Supported by National "973" Project of China (2006CB102105)National "863" Project of China (2006AA10A120)Earmarked Fund for Modern Agro-industry Technology Research System (nycytx-42-G1-07)
文摘Bone morphogenetic protein-2 （BMP-2） plays a key role in bone formation and maintenance. BMP-2 can stimulate longitudinal bone growth by increasing the growth plate chondrocyte proliferation, hypertrophy, and cartilage matrix synthesis. The current study was designed to investigate the associations of the BMP-2 gene polymorphism with chicken skeletal traits. Northeast Agricultural University F2 resource population was used in this study. Body weight and body composition traits were measured in F2 population. Polymorphism between parental lines was detected by DNA sequencing, and PCR-fragment length polymorphism method was then developed to screen the population. The results showed that the BMP-2 gene polymorphism was associated with skeletal traits in F2 population. This research suggests that BMP-2 gene may be a candidate locus or linked to a major gene that affects skeletal traits in chickens.
基金Supported by Jiangsu Natural Science FoundationYangzhou University Natural Science Fund through Grant (BK2006068 and NK0513129)
文摘The study aims to analyze the distribution of the 313 bp indel （insertion/deletion termed as indel） in first intron of POU1F1 and it＇s association with reproduction traits in Sutai pigs by using the PCR-DSCP technique. The results showed that in this commercial pig population, the frequency of allele A was 0.6371, B was 0.3629; the genotype frequency of AA was 0.4516, AB was 0.3710, BB was 0.1774, and the Z2 test showed that the allele frequencies were in Hardy-Weinberg equilibrium. The SPSS GLM procedure was used to identify the association of the 313 bp indel with reproductive traits. In Sutai pigs, the pigs with AA genotype represented higher value in all reproduction traits, except for higher survival rate of piglets at weaning. Higher weaning weight was significantly associated with AA genotype pigs and higher survival rate of piglets at weaning was significantly associated with BB genotype （P〈0.05）, but no significant differences in other reproduction traits among genotypes were found （P〉0.05）; the P value of different traits affected by fixed factors were not significant as well （P〉0.05）. The result indicated that although this 313 bp indel was significantly associated with the weaning weight and survival rate at weaning, no any association with major reproduction traits was observed in Sutai pigs.
文摘Correction:J Cotton Res 7,20(2024)https://doi.org/10.1186/s42397-024-00180-3 Following publication of the original article(Shui et al.2024),the author found 5 errors in the published article.1.One of the author’s name has been corrected from Gou Chunping to Guo Chunping.2.The reference(Zhao SQ.2016)in Table 2 has been updated to:Zhao SQ.Analysis on the major gene and multigene mixed inheritance and QTL mapping for early maturity traits in upland cotton.Chin Acad Agric Sci.2016.https://doi.org/10.3969/j.issn.201600501.(in Chinese with English abstract).3.In’Results’part,’Phenotype analysis of 238 cotton boll abscission among cotton accessions’paragraph,the phenotype analysis of AR1 ranging from 19.27%–63.79%,which was wrongly written as 19.27%-63.97%.4.The‘2018KRL’is modified to‘2018KEL’in Table 1.
文摘1.Development of EST-SSRs derived from G.barbadense:One hundred and nineteen EST-SSRs were developed based on 98 unique ESTs from a cDNA library constructed in our laboratory using developing fibers from G.barbadense cv.3-79.Among the SSRs,trinucleotide AAG appeared
基金Supported by the Key Research and Development Project of Heilongjiang Province(GA21B009-06)。
文摘Improvement of seed yield of soybean(Glycine max(L.)Merr.)is generally achieved by combining morphological and yield-related traits,such as plant height(PH),node number on main stem(NN),pod number per plant(NP),seed number per plant(NS),100-seed weight(HSW)and seed weight per plant(SWPP).Identifying quantitative trait loci(QTLs)for morphological and yield-related traits is therefore important for breeding.In this study,a four-way recombinant inbred line population comprising 160 lines derived from the cross(Kenfeng14×Kenfeng15)×(Heinong48×Kenfeng19)was planted in five different environments and morphological and yield-related trait data were used to identify QTLs by the inclusive composite interval mapping method.Totally 38 QTLs for PH,40 QTLs for NN,26 QTLs for NP,10 QTLs for NS,26 QTLs for HSW and 49 QTLs for SWPP were detected in 125 genomic regions.Single QTLs explained 2.17%-14.60%,2.00%-10.04%,2.37%-9.77%,2.62%-8.61%,0.47%-6.51%and 0.14%-12.39%of the phenotypic variation for PH,NN,NP,NS,HSW and SWPP,respectively.Among these 125 genomic regions,120 were newly associated with morphological and yield-related traits.The results would facilitate the molecular breeding of morphological and yield-related traits in soybean.
文摘Cotton(Gossypium spp.) is the leading fiber crop,and an important source of the important edible oil and protein meals in the world.Complex genetics and strong environmental effects hinder
