An in-memory storage system provides submillisecond latency and improves the concurrency of user applications by caching data into memory from external storage.Fault tolerance of in-memory storage systems is essential...An in-memory storage system provides submillisecond latency and improves the concurrency of user applications by caching data into memory from external storage.Fault tolerance of in-memory storage systems is essential,as the loss of cached data requires access to data from external storage,which evidently increases the response latency.Typically,replication and erasure code(EC)are two fault-tolerant schemes that pose different trade-offs between access performance and storage usage.To help make the best performance and space trade-off,we design ElasticMem,a hybrid fault-tolerant distributed in-memory storage system that supports elastic redundancy transition to dynamically change the fault-tolerant scheme.ElasticMem exploits a novel EC-oriented replication(EOR)that carefully designs the data placement of replication according to the future data layout of EC to enhance the I/O efficiency of redundancy transition.ElasticMem solves the consistency problem caused by concurrent data accesses via a lightweight table-based scheme combined with data bypassing.It detects correlated read and write requests and serves subsequent read requests with local data.We implement a prototype that realizes ElasticMem based on Memcached.Experiments show that ElasticMem remarkably reduces the time of redundancy transition,the overall latency of correlated concurrent data accesses,and the latency of single data access among them.展开更多
Background Soil available phosphorus(AP)deficiency significantly limits cotton production,particularly in arid and saline-alkaline regions.Screening cotton cultivars for low phosphorus(P)tolerance is crucial for the s...Background Soil available phosphorus(AP)deficiency significantly limits cotton production,particularly in arid and saline-alkaline regions.Screening cotton cultivars for low phosphorus(P)tolerance is crucial for the sustainable development of cotton production.However,the effect of different growth media on the screening outcomes remains unclear.To address this,we evaluated the low P tolerance of 25 cotton cultivars through hydroponic culture at two P levels(0.01 and 0.5 mmol·L^(-1) KH_(2)PO_(4))in 2018 and field culture with two P rates(0 and 90 kg·hm^(-2),in P2O5)in 2019.Results In the hydroponic experiments,principal component analysis(PCA)showed that shoot dry weight(SDW)and P utilization efficiency in shoots(PUES)of cotton seedlings explained over 45%of the genetic variation in P nutri-tion.Cotton cultivars were subjected to comprehensive cluster analysis,utilizing agronomic traits(SDW and PUES)during the seedling stage(hydroponic)and yield and fiber quality traits during the mature stage(in field).These cultivars were grouped into four clusters:resistant,moderately resistant,moderately sensitive,and sensitive.In low P conditions(0.01 mmol·L^(-1) KH_(2)PO_(4) and 4.5 mg·kg^(-1) AP),the low-P-resistant cluster showed significantly smaller reduc-tions in SDW(54%),seed cotton yield(3%),lint yield(-2%),fiber length(-1)%),and fiber strength(-3%)compared with the low-P-sensitive cluster(75%,13%,17%,7%,and 9%,respectively).The increase in PUES(299%)in the resist-ant cluster was also significantly higher than in the sensitive cluster(131%).Four of the eight low-P-tolerant cotton cultivars identified in the field and six in the hydroponic screening overlapped in both screenings.Two cultivars overlapped in both screening in the low-P-sensitive cluster.Conclusion Based on the screenings from both field and hydroponic cultures,ZM-9131,CCRI-79,JM-958,and J-228 were identified as low-P-tolerant cotton cultivars,while JM-169,XM-33B,SCRC-28,and LNM-18 were identified as low P-sensitive cotton cultivars.The relationship between field and hydroponic screening results for low-P-tolerant cotton cultivars was strong,although field validation is still required.The low P tolerance of these cultivars was closely associ-ated with SDW and PUES.展开更多
To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four differen...To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four different types of silicon formulations,Si-50-G,Si-60-G,Si-T-G,and Si-E-G,were applied as foliar sprays at the seedling stage,and a control group CK(equal amount of distilled water)was set up.One week after the first silicon spray,two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12℃/10℃for 2,4,6,and 8 days.The effects of different silicon formulations on the chlorophyll,proline(Pro)and soluble sugar contents as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice seedlings.The results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves,with Si-50-G being the most effective,with a significant increment of 40.17%compared to the CK at 2 days of low temperature.Four silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 days.For Song Japonica 16,the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2,4 and 8 days of temperature stress,with 73.58%,20.95%and 217.24%increases compared to the CK,respectively.For 4 and 6 days of temperature stress,the most significant increase in CAT activity was observed in Si-E-G treatment,with 25.70%and 75.78%increases compared to the CK,respectively.For Dongnong 427,the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress,with significant increases of 58.15%and 82.76%compared to the CK,respectively,and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature stress,with significant increases of 97.75%and 245.10%compared to the CK,respectively.It showed that the spraying of silicon formulations could significantly enhance the cold tolerance of rice.This study provided a scientific basis for the rational use of silicon formulations to enhance cold tolerance in rice and had important theoretical and practical significance for ensuring sustainable high and stable rice yields in Heilongjiang Province,as well as for the development of silicon fertilizers.展开更多
Background: Salinity is a major abiotic stress to global agriculture which hampers crop growth and development, and eventually reduces yield. Transgenic technology is an e ective and e cient approach to improve crop s...Background: Salinity is a major abiotic stress to global agriculture which hampers crop growth and development, and eventually reduces yield. Transgenic technology is an e ective and e cient approach to improve crop salt tolerance but depending on the availability of e ective genes. We previously isolated Salt Tolerance5(ThST5) from the halophyte Thellungiella halophila, an ortholog of Arabidopsis SPT4-2 which encodes a transcription elongation factor. However, SPT4-2-confered salt tolerance has not been evaluated in crops yet. Here we report the evaluation of Th ST5-conferred salt tolerance in cotton(Gossypium hirsutum L.).Results: The ThST5 overexpression transgenic cotton plants displayed enhanced tolerance to salt stress during seed germination and seedling stage compared with wild type. Particularly, the transgenic plants showed improved salinity tolerance as well as yield under saline field conditions. Comparative transcriptomic analysis showed that ThST5 improved salt tolerance of transgenic cotton mainly by maintaining ion homeostasis. In addition, ThST5 also orchestrated the expression of genes encoding antioxidants and salt-responsive transcription factors.Conclusion: Our results demonstrate that ThST5 is a promising candidate to improve salt tolerance in cotton.展开更多
The essential requirements of the end-effector of large space manipulator are capabilities of misalignment tolerance and soft capture.According to these requirements,an end-effector prototype combining the tendon-shea...The essential requirements of the end-effector of large space manipulator are capabilities of misalignment tolerance and soft capture.According to these requirements,an end-effector prototype combining the tendon-sheath transmission system with steel cable snaring mechanism was manufactured.An analysis method based on the coordinate transformation and the projection of key points of the mechanical interface was proposed,and it was a guideline of the end-effector design.Furthermore,the tendon-sheath transmission system was employed in the capture subassembly to reduce the inertia of the capture mechanism and enlarge the capture space.The capabilities of misalignment tolerance and soft capture were validated through the dynamic simulation in ADAMS software.The results of the capture simulation and experiment show that the end-effector has outstanding capabilities of misalignment tolerance and soft capture.The translation misalignments in radial directions are±100 mm,and angular misalignments about pitch and yaw are±15°.展开更多
In this paper, novel mathematical expressions are derived for the Global Positioning System (GPS) receiver interference tolerance in the presence of different types of interference signals such as: continuous wave int...In this paper, novel mathematical expressions are derived for the Global Positioning System (GPS) receiver interference tolerance in the presence of different types of interference signals such as: continuous wave interference, narrowband interference, partial band interference, broadband interference, match spectrum interference and pulse interference. Also, in this paper the mean time to loss lock is determined in order to analyse the mentioned interferences effect on the GPS receiver. These derived analytical expressions are validated with the aid of extensive simulation experiments.展开更多
Tolerance sensitivity limits the practical application of the cross-eye jammer.Previous literature has demonstrated that retrodirective cross-eye jamming with multiple antenna elements possesses the advantage of loose...Tolerance sensitivity limits the practical application of the cross-eye jammer.Previous literature has demonstrated that retrodirective cross-eye jamming with multiple antenna elements possesses the advantage of loose tolerance requirements compared to traditional cross-eye jamming.However,the previous analysis was limited,because there are still some factors affecting the parameter tolerance of the multiple-element retrodirective cross-eye jamming(MRCJ)system and they have not been investigated completely,such as the loop difference,the baseline ratio and the jammer-to-signal ratio.This paper performs a comprehensive tolerance analysis of the MRCJ system with a nonuniformspacing linear array.Simulation results demonstrate the tolerance effects of the above influence factors and give reasonable advice for easing tolerance sensitivity.展开更多
Total 75 rice varieties （lines） in Heilongjiang Province （or cold region） as germplasm resources were identified for cold tolerance at germinating stage by controlling temperature in artificial incubator. The resu...Total 75 rice varieties （lines） in Heilongjiang Province （or cold region） as germplasm resources were identified for cold tolerance at germinating stage by controlling temperature in artificial incubator. The results showed that the shooting seed rate at the germinating stage could be used as the evaluation index of cold tolerance. The cold tolerance was recorded on 1-9 scale and could be identified by the criteria of five indexes such as highly tolerant （HT）, tolerant （T）, moderately tolerant （MT）, susceptible （S）, highly susceptible （HS）.展开更多
The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on co...The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings （chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81） under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.展开更多
This paper studies the effect of amplitude-phase errors on the antenna performance. Via builting on a worst-case error tolerance model, a simple and practical worst error tolerance analysis based on the chaos-genetic ...This paper studies the effect of amplitude-phase errors on the antenna performance. Via builting on a worst-case error tolerance model, a simple and practical worst error tolerance analysis based on the chaos-genetic algorithm （CGA） is proposed. The proposed method utilizes chaos to optimize initial population for the genetic algorithm （GA） and introduces chaotic disturbance into the genetic mutation, thereby improving the ability of the GA to search for the global optimum. Numerical simulations demonstrate that the accuracy and stability of the worst-case analysis of the proposed approach are superior to the GA. And the proposed algorithm can be used easily for the error tolerant design of antenna arrays.展开更多
Fourteen inbreds of maize were tested for the tolerance to chlorsulfuron with the method of seedling water culter.Significant difference existed in the tolerance of different type maize when tested with 1 and 2 μg/kg...Fourteen inbreds of maize were tested for the tolerance to chlorsulfuron with the method of seedling water culter.Significant difference existed in the tolerance of different type maize when tested with 1 and 2 μg/kg of chlorsulfuron.The tolerance in different type maize wa dent>flint>sugar>midiem>super sugarpop.The tolerance of different inbred maize was negative correlated with the concentration concentration of chorsulfuron.The tolerance of hybrids might have some relation with their parent lines because in this test the hybrids response to chlorsulfuron showed significant positive correlation with that of tits female parent which suggented some maternal in inheritance and was not controlled by nuclear.It was possible that tolerance of maize to chlorsulfuron was cytoplasm inheritance.展开更多
Winter wheat （Triticum aestivum） cultivars Dongnongdongmai 1 with strong cold/freezing tolerance and Jimai 22 with weak cold/freezing tolerance were used for investigating the difference of microstructure and ultras...Winter wheat （Triticum aestivum） cultivars Dongnongdongmai 1 with strong cold/freezing tolerance and Jimai 22 with weak cold/freezing tolerance were used for investigating the difference of microstructure and ultrastructure between leaves of two cultivars under low temperature stress （5℃ and -15 ℃） using optical and electron microscope. The results showed that there was no obvious difference between leaves of Dongnongdongmai 1 and Jimai 22 in microstructure. However, the difference between those leaves was distinct in ultrastructure. The grana lamella and stroma lamella were stacked regularly and arranged parallelly along the long axis of chloroplasts in cv. Dongnongdongmai 1, while the arrangement directions of thylakoids in Jimai 22＇s leaves were so irregular as to form various angles with the long axis of chloroplasts. At -15℃, the mitochondrias were swelled to be round and the structure of cristaes became blurry in both cultivars＇ leaves, while some cristaes of Jimai 22 disappeared. These results would provide theoretical evidence for selecting cold/freezing tolerant winter wheat germplasm resources展开更多
MicroRNAs （miRNAs） are one kind of small RNA in all eukaryote. MicroRNAs can regulate gene expression of eukaryote; they widely participate in every physiological process. They can block mRNA expression or cleave mR...MicroRNAs （miRNAs） are one kind of small RNA in all eukaryote. MicroRNAs can regulate gene expression of eukaryote; they widely participate in every physiological process. They can block mRNA expression or cleave mRNA by complement to target mRNA. Scholars estimate miRNA genes occuping about 1% of genome, but they can regulate 10%-30% genes of whole genome. The genes are regulated by miRNA including signal proteins, enzymes, transcription factors, and so on. In the field of plant research, the start of miRNA research is later, but it is proved that plant miRNAs are important to every plant physiological process. Now miRNA has become the hotspot of plant molecular biology research. This paper introduced the biology function, action mechanism, researching method and recently development of microRNAs, also focused on advances in plant microRNAs. This paper has important reference value for plant stress tolerance miRNA research.展开更多
Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton ...Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton production due to a lack of sufficient water for its cultivation.Effects of drought stress are estimated to affect more than 50%of the cotton growing regions.To elucidate the drought tolerance phenomenon in cotton,a backcross population was developed from G.tomentosum,a drought tolerant donor parent and G.hirsutum which is highly susceptible to drought stress.Results:A genetic map of 10888 SNP markers was developed from 200 BC_2F_2 populations.The map spanned 4191.3 centi-Morgan(c M),with an average distance of 0.1047 c M,covering 51%and 49%of At and Dt sub genomes,respectively.Thirty stable Quantitative trait loci(QTLs)were detected,in which more than a half were detected in the At subgenome.Eighty-nine candidate genes were mined within the QTL regions for three traits:cell membrane stability(CMS),saturated leaf weight(SLW)and chlorophyll content.The genes had varied physiochemical properties.A majority of the genes were interrupted by introns,and only 15 genes were intronless,accounting for 17%of the mined genes.The genes were found to be involved molecular function(MF),cellular component(CC)and biological process(BP),which are the main gene ontological(GO)functions.A number of mi RNAs were detected,such as mi R164,which is associated with NAC and MYB genes,with a profound role in enhancing drought tolerance in plants.Through RT-q PCR analysis,5 genes were found to be the key genes involved in enhancing drought tolerance in cotton.Wild cotton harbors a number of favorable alleles,which can be exploited to aid in improving the narrow genetic base of the elite cotton cultivars.The detection of 30 stable QTLs and 89 candidate genes found to be contributed by the donor parent,G.tomentosum,showed the significant genes harbored by the wild progenitors which can be exploited in developing more robust cotton genotypes with diverse tolerance levels to various environmental stresses.Conclusion:This was the first study involving genome wide association mapping for drought tolerance traits in semi wild cotton genotypes.It offers an opportunity for future exploration of these genes in developing highly tolerant cotton cultivars to boost cotton production.展开更多
A new approach to knowledge acquisition in incomplete information system with fuzzy decisions is proposed. In such incomplete information system, the universe of discourse is classified by the maximal tolerance classe...A new approach to knowledge acquisition in incomplete information system with fuzzy decisions is proposed. In such incomplete information system, the universe of discourse is classified by the maximal tolerance classes, and fuzzy approximations are defined based on them. Three types of relative reducts of maximal tolerance classes are then proposed, and three types of fuzzy decision rules based on the proposed attribute description are defined. The judgment theorems and approximation discernibility functions with respect to them are presented to compute the relative reduct by using Boolean reasoning techniques, from which we can derive optimal fuzzy decision rules from the systems. At last, three types of relative reducts of the system and their computing methods are given.展开更多
A long-red awn weedy rice and rice cultivars named Ken99004（ZCI）, Crossing-503（ZC2）, Ken Sticky Rice（ZC3）, Shashani（ZC4）, Long-Grain Aromatic Rice（ZC5） were used to study typical wild traits of weedy rice an...A long-red awn weedy rice and rice cultivars named Ken99004（ZCI）, Crossing-503（ZC2）, Ken Sticky Rice（ZC3）, Shashani（ZC4）, Long-Grain Aromatic Rice（ZC5） were used to study typical wild traits of weedy rice and to identify the cold resistance of all genotypes. The results showed that the stem of long-red awn weedy rice was the weakest. No difference was found in the early stage of rice growth, e.g. the vegetable growth stage, between long-red awn weedy rice and other rice cultivars in the stem rigidity, but in the later stage, or reproductive growth stage, their leaves and stems were senescent rapidly because of the speedy supply of nutrients for panicle growth, meanwhile the rigidity of stem was reduced sharply just as withered weeds. The germination rate of long- red awn weedy rice was the highest in cold condition, and in turn were ZCI, ZC2, ZC3, ZC4, ZC5, respectively. The performances of genotypes in cold tolerance were identical, those with a high ability of germination in low temperature also showed a strong cold tolerance in main traits in whole growth period, the order from strong to weak in the extent of cold tolerance were long-red awn weedy rice, ZCl, ZC2, ZC3, ZC4, ZC5, respectively展开更多
Background: Gossypium hirsutum(upland cotton) is one of the principal fiber crops in the world. Cotton yield is highly affected by abiotic stresses, among which salt stress is considered as a major problem around the ...Background: Gossypium hirsutum(upland cotton) is one of the principal fiber crops in the world. Cotton yield is highly affected by abiotic stresses, among which salt stress is considered as a major problem around the globe. Transgenic approach is efficient to improve cotton salt tolerance but depending on the availability of salt tolerance genes.Results: In this study we evaluated salt tolerance candidate gene ST7 from Thellungiella halophila, encoding a homolog of Arabidopsis aluminum-induced protein, in cotton. Our results showed that ThST7 overexpression in cotton improved germination under NaCl stress as well as seedling growth. Our field trials also showed that ThST7 transgenic cotton lines produced higher yield under salt stress conditions. The improved salt tolerance of the transgenic cotton lines was partially contributed by enhanced antioxidation as shown by diaminobenzidine(DAB) and nitrotetrazolium blue chloride(NBT) staining. Moreover, transcriptomic analysis of ThST7 overexpression lines showed a significant upregulation of the genes involved in ion homeostasis and antioxidation, consistent with the salt tolerance phenotype of the transgenic cotton.Conclusions: Our results demonstrate that ThST7 has the ability to improve salt tolerance in cotton. The ThST7 transgenic cotton may be used in cotton breeding for salt tolerance cultivars.展开更多
Background: Cotton is an important commercial crop for being a valuable source of natural fiber.Its production has undergone a sharp decline because of abiotic stresses,etc.Drought is one of the major abiotic stress c...Background: Cotton is an important commercial crop for being a valuable source of natural fiber.Its production has undergone a sharp decline because of abiotic stresses,etc.Drought is one of the major abiotic stress causing significant yield losses in cotton.However,plants have evolved self-defense mechanisms to cope abiotic factors like drought,salt,cold,etc.The evolution of stress responsive transcription factors such as the trihelix,a nodule-inception-like protein(NLP),and the late embryogenesis abundant proteins have shown positive response in the resistance improvement to several abiotic stresses.Results: Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding(LHC)genes were carried out in cotton under drought stress conditions.A hundred and nine proteins encoded by the LHC genes were found in the cotton genome,with 55,27,and 27 genes found to be distributed in Gossypium hirsutum,G.arboreum,and G.raimondii,respectively.The proteins encoded by the genes were unevenly distributed on various chromosomes.The Ka/Ks(Non-synonymous substitution rate/Synonymous substitution rate)values were less than one,an indication of negative selection of the gene family.Differential expressions of genes showed that majority of the genes are being highly upregulated in the roots as compared with leaves and stem tissues.Most genes were found to be highly expressed in MR-85,a relative drought tolerant germplasm.Conclusion: The results provide proofs of the possible role of the LHC genes in improving drought stress tolerance,and can be explored by cotton breeders in releasing a more drought tolerant cotton varieties.展开更多
Background:Soil salinity seriously affects cotton growth,leading to the reduction of yield and fiber quality.Recently,genetic engineering has become an efficient tool to increase abiotic stress tolerance in crops.Resu...Background:Soil salinity seriously affects cotton growth,leading to the reduction of yield and fiber quality.Recently,genetic engineering has become an efficient tool to increase abiotic stress tolerance in crops.Results:In this study,isopentyl transferase(IPT),a key enzyme involved in cytokinin(CTK) biosynthesis from Agrobacterium tumefaciens,was selected to generate transgenic cotton via Agrobacterium-mediated transformation.A senescence-inducible SAG12 promoter from Arabidopsis was fused with the IPT gene.Ectopic-expression of SAG12::IPT significantly promoted seed germination or seedling tolerance to salt stress.Two IPTtransgenic lines,OE3 as a tolerant line during seed germination,and OE8 as a tolerant line at seedling stage,were selected for further physiological analysis.The data showed that ectopic-expression of SAG12::IPT induced the accumulation of CTKs not only in leaves and roots,but also in germinating seeds.Moreover,ectopic-expressing IPT increased the activity of antioxidant enzymes,which was associated with the less reactive oxygen species(ROS) accumulation compared with control plants.Also,ectopic-expression of IPT produced higher K^+/Na^+ratio in cotton shoot and root Conclusion:The senescence-induced CTK accumulation in cotton seeds and seedlings positively regulates salt stress partially by elevating ROS scavenging capability.展开更多
OBJECTIVE Considerable effort has recently been directed at developing multifunctional opioid drugs as an alternative strategy to minimize the unwanted side effects of opioid analgesics.We recently developed a novel m...OBJECTIVE Considerable effort has recently been directed at developing multifunctional opioid drugs as an alternative strategy to minimize the unwanted side effects of opioid analgesics.We recently developed a novel multifunctional agonist for opioid and neuropeptide FF(NPFF) receptors named DN-9.The present study was conducted to evaluate the pharmacological activities of DN-9 after peripheral administration.METHODS Antinociceptive activities of subcutaneous DN-9 were investigated in mouse models of acute inflammatory and neuropathic pain.Furthermore,the side-effects of DN-9 were evaluated after peripheral injection in rotarod,antinociceptive tolerance,abuse and gastrointestinal transit tests.RESULTS Subcutaneous DN-9 dose-dependently produced antinociception via peripheral mu-and kappa-opioid receptors,independent of delta-opioid and NPFF receptors,in the tail-flick assay.Similarly,a dose-dependent antinociceptive effect of DN-9 was mediated via peripheral opioid receptors in other inflammatory and neuropathic pain models.Repeated treatment with DN-9 produced antinociceptive effects without a loss of potency in various models of acute,inflammatory and neuropathic pain.DN-9 maintained potent analgesia in morphine-tolerant mice.The gastrointestinal motility inhibition and abuse properties of DN-9 were significantly reduced after subcutaneous injection compared to morphine.DN-9 did not significantly influence the motor coordination of mice.CONCLUSION Subcutaneous administration of DN-9 produces potent analgesic activities with minimal side effects.These data strengthen the therapeutic potential of peripherally acting opioids with multifunctional agonistic properties that are active in a broad range of experimental pain models after peripheral delivery.展开更多
基金supported by the Fundamental Research Funds for the Central Universities(WK2150110022)Anhui Provincial Natural Science Foundation(2208085QF189)National Natural Science Foundation of China(62202440).
文摘An in-memory storage system provides submillisecond latency and improves the concurrency of user applications by caching data into memory from external storage.Fault tolerance of in-memory storage systems is essential,as the loss of cached data requires access to data from external storage,which evidently increases the response latency.Typically,replication and erasure code(EC)are two fault-tolerant schemes that pose different trade-offs between access performance and storage usage.To help make the best performance and space trade-off,we design ElasticMem,a hybrid fault-tolerant distributed in-memory storage system that supports elastic redundancy transition to dynamically change the fault-tolerant scheme.ElasticMem exploits a novel EC-oriented replication(EOR)that carefully designs the data placement of replication according to the future data layout of EC to enhance the I/O efficiency of redundancy transition.ElasticMem solves the consistency problem caused by concurrent data accesses via a lightweight table-based scheme combined with data bypassing.It detects correlated read and write requests and serves subsequent read requests with local data.We implement a prototype that realizes ElasticMem based on Memcached.Experiments show that ElasticMem remarkably reduces the time of redundancy transition,the overall latency of correlated concurrent data accesses,and the latency of single data access among them.
基金the Natural Science Foundation of Xinjiang Uygur Autonomous Region(2024D01A56)the National Key Research and Develop-ment Program of China(2017YFD0201906)+2 种基金the Central Research Institutes of Basic Research and the Public Service Special Foundation(1610162022044)the China Agriculture Research System(CARS-15-11)the Agricultural Sci-ence and Technology Innovation Program of Chinese Academy of Agricultural Sciences.
文摘Background Soil available phosphorus(AP)deficiency significantly limits cotton production,particularly in arid and saline-alkaline regions.Screening cotton cultivars for low phosphorus(P)tolerance is crucial for the sustainable development of cotton production.However,the effect of different growth media on the screening outcomes remains unclear.To address this,we evaluated the low P tolerance of 25 cotton cultivars through hydroponic culture at two P levels(0.01 and 0.5 mmol·L^(-1) KH_(2)PO_(4))in 2018 and field culture with two P rates(0 and 90 kg·hm^(-2),in P2O5)in 2019.Results In the hydroponic experiments,principal component analysis(PCA)showed that shoot dry weight(SDW)and P utilization efficiency in shoots(PUES)of cotton seedlings explained over 45%of the genetic variation in P nutri-tion.Cotton cultivars were subjected to comprehensive cluster analysis,utilizing agronomic traits(SDW and PUES)during the seedling stage(hydroponic)and yield and fiber quality traits during the mature stage(in field).These cultivars were grouped into four clusters:resistant,moderately resistant,moderately sensitive,and sensitive.In low P conditions(0.01 mmol·L^(-1) KH_(2)PO_(4) and 4.5 mg·kg^(-1) AP),the low-P-resistant cluster showed significantly smaller reduc-tions in SDW(54%),seed cotton yield(3%),lint yield(-2%),fiber length(-1)%),and fiber strength(-3%)compared with the low-P-sensitive cluster(75%,13%,17%,7%,and 9%,respectively).The increase in PUES(299%)in the resist-ant cluster was also significantly higher than in the sensitive cluster(131%).Four of the eight low-P-tolerant cotton cultivars identified in the field and six in the hydroponic screening overlapped in both screenings.Two cultivars overlapped in both screening in the low-P-sensitive cluster.Conclusion Based on the screenings from both field and hydroponic cultures,ZM-9131,CCRI-79,JM-958,and J-228 were identified as low-P-tolerant cotton cultivars,while JM-169,XM-33B,SCRC-28,and LNM-18 were identified as low P-sensitive cotton cultivars.The relationship between field and hydroponic screening results for low-P-tolerant cotton cultivars was strong,although field validation is still required.The low P tolerance of these cultivars was closely associ-ated with SDW and PUES.
基金the National Natural Science Foundation of China(31471440)。
文摘To investigate the effects of silicon formulations on the cold tolerance of rice seedlings,Song Japonica 16(not cold tolerant)and Dongnong 427(cold tolerant)rice varieties were used as test materials and four different types of silicon formulations,Si-50-G,Si-60-G,Si-T-G,and Si-E-G,were applied as foliar sprays at the seedling stage,and a control group CK(equal amount of distilled water)was set up.One week after the first silicon spray,two types of rice were subjected to low-temperature stress treatments at day/night temperatures of 12℃/10℃for 2,4,6,and 8 days.The effects of different silicon formulations on the chlorophyll,proline(Pro)and soluble sugar contents as well as superoxide dismutase(SOD),peroxidase(POD)and catalase(CAT)activities of rice seedlings under low-temperature stress were compared to find out the effects of silicon formulations on the cold tolerance of rice seedlings.The results showed that silicon formulations could significantly increase the chlorophyll content of rice seedling leaves,with Si-50-G being the most effective,with a significant increment of 40.17%compared to the CK at 2 days of low temperature.Four silicon formulations significantly increased the proline content and soluble sugar content of rice leaves at low temperature for 4-8 days.For Song Japonica 16,the most significant increment in leaf POD activity was observed in Si-E-G treatment at 2,4 and 8 days of temperature stress,with 73.58%,20.95%and 217.24%increases compared to the CK,respectively.For 4 and 6 days of temperature stress,the most significant increase in CAT activity was observed in Si-E-G treatment,with 25.70%and 75.78%increases compared to the CK,respectively.For Dongnong 427,the Si-60-G treatment showed the highest increase in leaf SOD activity for 4 and 8 days of temperature stress,with significant increases of 58.15%and 82.76%compared to the CK,respectively,and the Si-E-G treatment showed the highest increase in leaf POD activity for 2 and 8 days of temperature stress,with significant increases of 97.75%and 245.10%compared to the CK,respectively.It showed that the spraying of silicon formulations could significantly enhance the cold tolerance of rice.This study provided a scientific basis for the rational use of silicon formulations to enhance cold tolerance in rice and had important theoretical and practical significance for ensuring sustainable high and stable rice yields in Heilongjiang Province,as well as for the development of silicon fertilizers.
基金supported by grants from the Ministry of Science and Technol-ogy of China(Grant No.2016ZX08005004-003).
文摘Background: Salinity is a major abiotic stress to global agriculture which hampers crop growth and development, and eventually reduces yield. Transgenic technology is an e ective and e cient approach to improve crop salt tolerance but depending on the availability of e ective genes. We previously isolated Salt Tolerance5(ThST5) from the halophyte Thellungiella halophila, an ortholog of Arabidopsis SPT4-2 which encodes a transcription elongation factor. However, SPT4-2-confered salt tolerance has not been evaluated in crops yet. Here we report the evaluation of Th ST5-conferred salt tolerance in cotton(Gossypium hirsutum L.).Results: The ThST5 overexpression transgenic cotton plants displayed enhanced tolerance to salt stress during seed germination and seedling stage compared with wild type. Particularly, the transgenic plants showed improved salinity tolerance as well as yield under saline field conditions. Comparative transcriptomic analysis showed that ThST5 improved salt tolerance of transgenic cotton mainly by maintaining ion homeostasis. In addition, ThST5 also orchestrated the expression of genes encoding antioxidants and salt-responsive transcription factors.Conclusion: Our results demonstrate that ThST5 is a promising candidate to improve salt tolerance in cotton.
基金Project(2006AA04Z228) supported by National Hi-tech Research and Development Program of China
文摘The essential requirements of the end-effector of large space manipulator are capabilities of misalignment tolerance and soft capture.According to these requirements,an end-effector prototype combining the tendon-sheath transmission system with steel cable snaring mechanism was manufactured.An analysis method based on the coordinate transformation and the projection of key points of the mechanical interface was proposed,and it was a guideline of the end-effector design.Furthermore,the tendon-sheath transmission system was employed in the capture subassembly to reduce the inertia of the capture mechanism and enlarge the capture space.The capabilities of misalignment tolerance and soft capture were validated through the dynamic simulation in ADAMS software.The results of the capture simulation and experiment show that the end-effector has outstanding capabilities of misalignment tolerance and soft capture.The translation misalignments in radial directions are±100 mm,and angular misalignments about pitch and yaw are±15°.
文摘In this paper, novel mathematical expressions are derived for the Global Positioning System (GPS) receiver interference tolerance in the presence of different types of interference signals such as: continuous wave interference, narrowband interference, partial band interference, broadband interference, match spectrum interference and pulse interference. Also, in this paper the mean time to loss lock is determined in order to analyse the mentioned interferences effect on the GPS receiver. These derived analytical expressions are validated with the aid of extensive simulation experiments.
基金the National Natural Science Foundation of China(61801488,61921001,61601008).
文摘Tolerance sensitivity limits the practical application of the cross-eye jammer.Previous literature has demonstrated that retrodirective cross-eye jamming with multiple antenna elements possesses the advantage of loose tolerance requirements compared to traditional cross-eye jamming.However,the previous analysis was limited,because there are still some factors affecting the parameter tolerance of the multiple-element retrodirective cross-eye jamming(MRCJ)system and they have not been investigated completely,such as the loop difference,the baseline ratio and the jammer-to-signal ratio.This paper performs a comprehensive tolerance analysis of the MRCJ system with a nonuniformspacing linear array.Simulation results demonstrate the tolerance effects of the above influence factors and give reasonable advice for easing tolerance sensitivity.
文摘Total 75 rice varieties （lines） in Heilongjiang Province （or cold region） as germplasm resources were identified for cold tolerance at germinating stage by controlling temperature in artificial incubator. The results showed that the shooting seed rate at the germinating stage could be used as the evaluation index of cold tolerance. The cold tolerance was recorded on 1-9 scale and could be identified by the criteria of five indexes such as highly tolerant （HT）, tolerant （T）, moderately tolerant （MT）, susceptible （S）, highly susceptible （HS）.
基金Supported by the Special Agricultural Project of Agricultural Department (200903003)the Agricultural Modernization Project in the Science and Technology Development Plan of Jilin Province (2009-2010) the High-yield Project of Science and Technology Department (2011BAD16B10)
文摘The study was designated to explore the physiological mechanism of cold tolerance enhanced by phosphate in rice. An experiment was conducted to investigate the effects of different levels of phosphate fertilizer on cold tolerance and its related physiological parameters in rice seedings （chilling-sensitive cv. Changbai 9 and chilling-tolerant cv. Jijing 81） under low temperature stress. At the same time, the identification of cold tolerance was conducted. Compared with the normal temperature treatment, the relative chlorophyll content, photosynthesis rate, Fv/Fm and qP decreased and index of unsaturated fatty acid increased in rice under low temperature stress. The effect of chilling-sensitive cultivars was more than that of chilling-tolerant cultivars, more phosphorus fertilizer properly improved seedling quality of rice, slowed relative chlorophyll content dropping degree of rice seeding, increased photosynthesis rate, Fv/Fm, qP and index of unsaturated fatty acids, and enhanced the ability to chilling-tolerant cultivars under low temperature. The effect on chilling-tolerant cultivars was significantly higher than that on chilling sensitive cultivars by applying more phosphorus fertilizer. Phosphate regulated photosynthetic physiology and membrane fluidity to reduce injury by low temperature, and increasd the cold tolerance capacity of rice.
基金supported by the National Natural Science Foundation of China (60901055)
文摘This paper studies the effect of amplitude-phase errors on the antenna performance. Via builting on a worst-case error tolerance model, a simple and practical worst error tolerance analysis based on the chaos-genetic algorithm （CGA） is proposed. The proposed method utilizes chaos to optimize initial population for the genetic algorithm （GA） and introduces chaotic disturbance into the genetic mutation, thereby improving the ability of the GA to search for the global optimum. Numerical simulations demonstrate that the accuracy and stability of the worst-case analysis of the proposed approach are superior to the GA. And the proposed algorithm can be used easily for the error tolerant design of antenna arrays.
文摘Fourteen inbreds of maize were tested for the tolerance to chlorsulfuron with the method of seedling water culter.Significant difference existed in the tolerance of different type maize when tested with 1 and 2 μg/kg of chlorsulfuron.The tolerance in different type maize wa dent>flint>sugar>midiem>super sugarpop.The tolerance of different inbred maize was negative correlated with the concentration concentration of chorsulfuron.The tolerance of hybrids might have some relation with their parent lines because in this test the hybrids response to chlorsulfuron showed significant positive correlation with that of tits female parent which suggented some maternal in inheritance and was not controlled by nuclear.It was possible that tolerance of maize to chlorsulfuron was cytoplasm inheritance.
基金Supported by Doctoral Scientific Research Project of Northeast Agricultural University (20082010)Innovative Research Team Project of Northeast Agricultural University (CXZ003)
文摘Winter wheat （Triticum aestivum） cultivars Dongnongdongmai 1 with strong cold/freezing tolerance and Jimai 22 with weak cold/freezing tolerance were used for investigating the difference of microstructure and ultrastructure between leaves of two cultivars under low temperature stress （5℃ and -15 ℃） using optical and electron microscope. The results showed that there was no obvious difference between leaves of Dongnongdongmai 1 and Jimai 22 in microstructure. However, the difference between those leaves was distinct in ultrastructure. The grana lamella and stroma lamella were stacked regularly and arranged parallelly along the long axis of chloroplasts in cv. Dongnongdongmai 1, while the arrangement directions of thylakoids in Jimai 22＇s leaves were so irregular as to form various angles with the long axis of chloroplasts. At -15℃, the mitochondrias were swelled to be round and the structure of cristaes became blurry in both cultivars＇ leaves, while some cristaes of Jimai 22 disappeared. These results would provide theoretical evidence for selecting cold/freezing tolerant winter wheat germplasm resources
文摘MicroRNAs （miRNAs） are one kind of small RNA in all eukaryote. MicroRNAs can regulate gene expression of eukaryote; they widely participate in every physiological process. They can block mRNA expression or cleave mRNA by complement to target mRNA. Scholars estimate miRNA genes occuping about 1% of genome, but they can regulate 10%-30% genes of whole genome. The genes are regulated by miRNA including signal proteins, enzymes, transcription factors, and so on. In the field of plant research, the start of miRNA research is later, but it is proved that plant miRNAs are important to every plant physiological process. Now miRNA has become the hotspot of plant molecular biology research. This paper introduced the biology function, action mechanism, researching method and recently development of microRNAs, also focused on advances in plant microRNAs. This paper has important reference value for plant stress tolerance miRNA research.
基金program was financially sponsored by the National Natural Science Foundation of China(31671745,31530053)the National key research and development plan(2016YFD0100306)。
文摘Background:Cotton is mainly grown for its natural fiber and edible oil.The fiber obtained from cotton is the indispensable raw material for the textile industries.The ever changing climatic condition,threatens cotton production due to a lack of sufficient water for its cultivation.Effects of drought stress are estimated to affect more than 50%of the cotton growing regions.To elucidate the drought tolerance phenomenon in cotton,a backcross population was developed from G.tomentosum,a drought tolerant donor parent and G.hirsutum which is highly susceptible to drought stress.Results:A genetic map of 10888 SNP markers was developed from 200 BC_2F_2 populations.The map spanned 4191.3 centi-Morgan(c M),with an average distance of 0.1047 c M,covering 51%and 49%of At and Dt sub genomes,respectively.Thirty stable Quantitative trait loci(QTLs)were detected,in which more than a half were detected in the At subgenome.Eighty-nine candidate genes were mined within the QTL regions for three traits:cell membrane stability(CMS),saturated leaf weight(SLW)and chlorophyll content.The genes had varied physiochemical properties.A majority of the genes were interrupted by introns,and only 15 genes were intronless,accounting for 17%of the mined genes.The genes were found to be involved molecular function(MF),cellular component(CC)and biological process(BP),which are the main gene ontological(GO)functions.A number of mi RNAs were detected,such as mi R164,which is associated with NAC and MYB genes,with a profound role in enhancing drought tolerance in plants.Through RT-q PCR analysis,5 genes were found to be the key genes involved in enhancing drought tolerance in cotton.Wild cotton harbors a number of favorable alleles,which can be exploited to aid in improving the narrow genetic base of the elite cotton cultivars.The detection of 30 stable QTLs and 89 candidate genes found to be contributed by the donor parent,G.tomentosum,showed the significant genes harbored by the wild progenitors which can be exploited in developing more robust cotton genotypes with diverse tolerance levels to various environmental stresses.Conclusion:This was the first study involving genome wide association mapping for drought tolerance traits in semi wild cotton genotypes.It offers an opportunity for future exploration of these genes in developing highly tolerant cotton cultivars to boost cotton production.
基金supported by the National Natural Science Foundation of China (61070241)the Natural Science Foundation of Shandong Province (ZR2010FM035)Science Research Foundation of University of Jinan (XKY0808)
文摘A new approach to knowledge acquisition in incomplete information system with fuzzy decisions is proposed. In such incomplete information system, the universe of discourse is classified by the maximal tolerance classes, and fuzzy approximations are defined based on them. Three types of relative reducts of maximal tolerance classes are then proposed, and three types of fuzzy decision rules based on the proposed attribute description are defined. The judgment theorems and approximation discernibility functions with respect to them are presented to compute the relative reduct by using Boolean reasoning techniques, from which we can derive optimal fuzzy decision rules from the systems. At last, three types of relative reducts of the system and their computing methods are given.
基金National Scientific Technology Key Project (2004BA907A27)Postdoctoral Fund Project of Heilongjiang Province
文摘A long-red awn weedy rice and rice cultivars named Ken99004（ZCI）, Crossing-503（ZC2）, Ken Sticky Rice（ZC3）, Shashani（ZC4）, Long-Grain Aromatic Rice（ZC5） were used to study typical wild traits of weedy rice and to identify the cold resistance of all genotypes. The results showed that the stem of long-red awn weedy rice was the weakest. No difference was found in the early stage of rice growth, e.g. the vegetable growth stage, between long-red awn weedy rice and other rice cultivars in the stem rigidity, but in the later stage, or reproductive growth stage, their leaves and stems were senescent rapidly because of the speedy supply of nutrients for panicle growth, meanwhile the rigidity of stem was reduced sharply just as withered weeds. The germination rate of long- red awn weedy rice was the highest in cold condition, and in turn were ZCI, ZC2, ZC3, ZC4, ZC5, respectively. The performances of genotypes in cold tolerance were identical, those with a high ability of germination in low temperature also showed a strong cold tolerance in main traits in whole growth period, the order from strong to weak in the extent of cold tolerance were long-red awn weedy rice, ZCl, ZC2, ZC3, ZC4, ZC5, respectively
基金supported by grants from Ministry of Science and Technology of China(Grant No.2016ZX08005004-003).
文摘Background: Gossypium hirsutum(upland cotton) is one of the principal fiber crops in the world. Cotton yield is highly affected by abiotic stresses, among which salt stress is considered as a major problem around the globe. Transgenic approach is efficient to improve cotton salt tolerance but depending on the availability of salt tolerance genes.Results: In this study we evaluated salt tolerance candidate gene ST7 from Thellungiella halophila, encoding a homolog of Arabidopsis aluminum-induced protein, in cotton. Our results showed that ThST7 overexpression in cotton improved germination under NaCl stress as well as seedling growth. Our field trials also showed that ThST7 transgenic cotton lines produced higher yield under salt stress conditions. The improved salt tolerance of the transgenic cotton lines was partially contributed by enhanced antioxidation as shown by diaminobenzidine(DAB) and nitrotetrazolium blue chloride(NBT) staining. Moreover, transcriptomic analysis of ThST7 overexpression lines showed a significant upregulation of the genes involved in ion homeostasis and antioxidation, consistent with the salt tolerance phenotype of the transgenic cotton.Conclusions: Our results demonstrate that ThST7 has the ability to improve salt tolerance in cotton. The ThST7 transgenic cotton may be used in cotton breeding for salt tolerance cultivars.
基金This research was funded by the National Natural Science Foundation of China,grant number 31621005,31530053,31671745The National Key R&D Program of China(2021YFE0101200),PSF/CRP/18thProtocol(07).
文摘Background: Cotton is an important commercial crop for being a valuable source of natural fiber.Its production has undergone a sharp decline because of abiotic stresses,etc.Drought is one of the major abiotic stress causing significant yield losses in cotton.However,plants have evolved self-defense mechanisms to cope abiotic factors like drought,salt,cold,etc.The evolution of stress responsive transcription factors such as the trihelix,a nodule-inception-like protein(NLP),and the late embryogenesis abundant proteins have shown positive response in the resistance improvement to several abiotic stresses.Results: Genome wide identification and characterization of the effects of Light-Harvesting Chloro a/b binding(LHC)genes were carried out in cotton under drought stress conditions.A hundred and nine proteins encoded by the LHC genes were found in the cotton genome,with 55,27,and 27 genes found to be distributed in Gossypium hirsutum,G.arboreum,and G.raimondii,respectively.The proteins encoded by the genes were unevenly distributed on various chromosomes.The Ka/Ks(Non-synonymous substitution rate/Synonymous substitution rate)values were less than one,an indication of negative selection of the gene family.Differential expressions of genes showed that majority of the genes are being highly upregulated in the roots as compared with leaves and stem tissues.Most genes were found to be highly expressed in MR-85,a relative drought tolerant germplasm.Conclusion: The results provide proofs of the possible role of the LHC genes in improving drought stress tolerance,and can be explored by cotton breeders in releasing a more drought tolerant cotton varieties.
基金supported by The Genetically Modified Organisms Breeding Major Projects of China Grant(2016ZX08005-004)
文摘Background:Soil salinity seriously affects cotton growth,leading to the reduction of yield and fiber quality.Recently,genetic engineering has become an efficient tool to increase abiotic stress tolerance in crops.Results:In this study,isopentyl transferase(IPT),a key enzyme involved in cytokinin(CTK) biosynthesis from Agrobacterium tumefaciens,was selected to generate transgenic cotton via Agrobacterium-mediated transformation.A senescence-inducible SAG12 promoter from Arabidopsis was fused with the IPT gene.Ectopic-expression of SAG12::IPT significantly promoted seed germination or seedling tolerance to salt stress.Two IPTtransgenic lines,OE3 as a tolerant line during seed germination,and OE8 as a tolerant line at seedling stage,were selected for further physiological analysis.The data showed that ectopic-expression of SAG12::IPT induced the accumulation of CTKs not only in leaves and roots,but also in germinating seeds.Moreover,ectopic-expressing IPT increased the activity of antioxidant enzymes,which was associated with the less reactive oxygen species(ROS) accumulation compared with control plants.Also,ectopic-expression of IPT produced higher K^+/Na^+ratio in cotton shoot and root Conclusion:The senescence-induced CTK accumulation in cotton seeds and seedlings positively regulates salt stress partially by elevating ROS scavenging capability.
基金National Natural Science Foundation of China(8167328281273355).
文摘OBJECTIVE Considerable effort has recently been directed at developing multifunctional opioid drugs as an alternative strategy to minimize the unwanted side effects of opioid analgesics.We recently developed a novel multifunctional agonist for opioid and neuropeptide FF(NPFF) receptors named DN-9.The present study was conducted to evaluate the pharmacological activities of DN-9 after peripheral administration.METHODS Antinociceptive activities of subcutaneous DN-9 were investigated in mouse models of acute inflammatory and neuropathic pain.Furthermore,the side-effects of DN-9 were evaluated after peripheral injection in rotarod,antinociceptive tolerance,abuse and gastrointestinal transit tests.RESULTS Subcutaneous DN-9 dose-dependently produced antinociception via peripheral mu-and kappa-opioid receptors,independent of delta-opioid and NPFF receptors,in the tail-flick assay.Similarly,a dose-dependent antinociceptive effect of DN-9 was mediated via peripheral opioid receptors in other inflammatory and neuropathic pain models.Repeated treatment with DN-9 produced antinociceptive effects without a loss of potency in various models of acute,inflammatory and neuropathic pain.DN-9 maintained potent analgesia in morphine-tolerant mice.The gastrointestinal motility inhibition and abuse properties of DN-9 were significantly reduced after subcutaneous injection compared to morphine.DN-9 did not significantly influence the motor coordination of mice.CONCLUSION Subcutaneous administration of DN-9 produces potent analgesic activities with minimal side effects.These data strengthen the therapeutic potential of peripherally acting opioids with multifunctional agonistic properties that are active in a broad range of experimental pain models after peripheral delivery.
