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Adulteration Recognition Between Taoren and Xingren by Hyperspectral Non-destructive Technology with Mixed Metaheuristics RBF-SVM Model
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作者 Xu Hongzhao Zhao Qinghe +2 位作者 Liu Huaxi Zhang Zifang Fang Junlong 《Journal of Northeast Agricultural University(English Edition)》 2025年第2期66-81,共16页
Taoren and Xingren are commonly used herbs in East Asian medicine with different medication functions but huge economic differences,and there are cases of adulterated sales in market transactions.An effective adultera... Taoren and Xingren are commonly used herbs in East Asian medicine with different medication functions but huge economic differences,and there are cases of adulterated sales in market transactions.An effective adulteration recognition based on hyperspectral technology and machine learning was designed as a non-destructive testing method in this paper.A hyperspectral dataset comprising 500 Taoren and 500 Xingren samples was established;six feature selection methods were considered in the modeling of radial basis function-support vector machine(RBF-SVM),whose interaction between the two optimization methods was further researched.Two mixed metaheuristics modeling methods,Mixed-PSO and Mixed-SA,were designed,which fused both band selection and hyperparameter optimization from two-stage into one with detailed process analysis.The metrics of this mixed model were improved by comparing with traditional two-stage method.The accuracy of Mixed-PSO was 89.2%in five-floods crossvalidation that increased 4.818%than vanilla RBF-SVM;the accuracy of Mixed-SA was 88.7%which could reach the same as the traditional two-stage method,but it only relied on 48 crux bands in full 100 bands in RBF-SVM model fitting. 展开更多
关键词 hyperspectral technology adulteration recognition machine learning
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基于EST-SSR的钟花樱与近缘种的聚类分析及嫁接亲和性研究
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作者 刘夔 李梓函 +4 位作者 张依 郝文洁 姜蕾 叶琦 付涛 《广西植物》 北大核心 2025年第4期761-772,共12页
该研究以16种樱属植物为研究材料,利用EST-SSR分子标记技术对其进行聚类分析,旨在为钟花樱及其近缘种的物种分类、物种资源保护、砧木选择和杂交亲本选择提供分子水平的依据和技术支持。结果表明:(1)遗传多样性分析发现17对EST-SSR引物... 该研究以16种樱属植物为研究材料,利用EST-SSR分子标记技术对其进行聚类分析,旨在为钟花樱及其近缘种的物种分类、物种资源保护、砧木选择和杂交亲本选择提供分子水平的依据和技术支持。结果表明:(1)遗传多样性分析发现17对EST-SSR引物共检测出98个等位基因,每对引物平均为5.76个,有效等位基因数(N_(e))为1.16~7.64,平均值为3.22;观察杂合度(H_(o))为0.04~0.54,平均值为0.28;期望杂合度(H_(e))为0.58~0.92,平均值为0.77;Shannon’s指数(I)为1.38~2.65,平均值为2.14;多态信息含量(PIC)为0.62~0.92,平均值为0.78。(2)聚类分析结果发现除了野生早樱和黑樱桃外,其余14个物种亲缘关系密切,聚为一大类(遗传相似系数C_(GS)为0.6531~0.9184),其中,钟花樱和高盆樱之间的亲缘关系最高(C_(GS)=0.9184),钟花樱和野生早樱之间的亲缘关系最低(C_(GS)=0.7755),因此,建议使用亲缘关系较远的野生早樱和黑樱桃与钟花樱进行杂交实验。(3)在我国南方的生产实践中,已出现以华中樱为砧木,以钟花樱为接穗嫁接的高接苗。因此,从理论上讲,与钟花樱亲缘关系较近的华中樱、山樱、散毛樱和浙闽樱等也可以作为砧木,但还应考虑砧木的抗性、长势、繁殖和寿命等。通过嫁接实验得知,华中樱和山樱作为砧木嫁接钟花樱成活率最高(≥80%),与分子实验基本一致,比较适合作为嫁接钟花樱的砧木。该研究结果为钟花樱的选育、繁殖、保护和利用以及樱属之间的物种分类提供了分子依据。 展开更多
关键词 樱属 钟花樱 遗传多样性 亲缘关系 育种 est-SSR
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基于RNA-seq的冰草EST-SSR标记开发及验证 被引量:1
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作者 杨东升 李冉 +4 位作者 宝格日乐 王海伟 卢倩倩 郝水源 刘建华 《麦类作物学报》 北大核心 2025年第7期891-901,共11页
冰草是小麦抗逆遗传改良的重要近缘植物,开发冰草基因组分子标记对于加快冰草的遗传育种效率和利用具有重要意义。本研究基于转录组测序(RNA-seq)技术,对2个冰草材料的幼嫩根、茎、叶片及结实期的花穗、种子进行生物信息学分析,开发高... 冰草是小麦抗逆遗传改良的重要近缘植物,开发冰草基因组分子标记对于加快冰草的遗传育种效率和利用具有重要意义。本研究基于转录组测序(RNA-seq)技术,对2个冰草材料的幼嫩根、茎、叶片及结实期的花穗、种子进行生物信息学分析,开发高多态性的EST-SSR标记并进行验证。结果表明,共获得23491793条高质量序列,其碱其长7.03 Gb,GC含量56.44%,Q30为93.04%。共获得33993条Unigene,总长度为29309958 bp。将所获Unigene与9大功能数据库进行比对,共有25614条Unigene获得功能注释,其中Nr数据库共注释24557条,GO数据库注释到66193条Unigene,按功能分为3个大类和42个亚类;共14412条Unigene注释到KEGG数据库的136条代谢通路;有11321条Unigene在KOG数据库获得注释,归属为25个功能类别;共预测到大于100 bp的CDS有7288条,SSR位点2443个,其中三碱基重复1439个,占总数的58.90%;获得EST-SSR多态性引物42对,从中随机选择10对在冰草的30个F_(2)代分离单株进行有效性验证,平均多态性比率为48.33%。以上结果说明利用RNA-seq技术大量开发的EST-SSR引物,可高效地应用于四倍体冰草产量、品质性状相关的标记开发、优异新种质指纹图谱构建及精准分子标记辅助育种。 展开更多
关键词 冰草 RNA-SEQ 生物信息学分析 est-SSR标记开发 验证
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Key Technology Research and Exemplification Analysis on Investigation and Assessment of Geoheritages and Geopark Construction
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作者 Guoqing Zhang~(1,2),Mingzhong Tian~2,Yuhuan Geng~2,Jing Guo~2 1.Faculty of Geosciences and Survey Engineering,East China Institute of Technology,Fuzhou 344000,China. 2.School of the Earth Sciences and Resources,China University of Goesciences(Beijing),Beijing 100083,China 《地学前缘》 EI CAS CSCD 北大核心 2009年第S1期291-291,共1页
In recent years,great efforts have been made on the geoparks’ application and construction,but there is a shortage of research on the theories and methodologies of geoheritage investigation and assessment. Based on t... In recent years,great efforts have been made on the geoparks’ application and construction,but there is a shortage of research on the theories and methodologies of geoheritage investigation and assessment. Based on the summaries of having finished geoheritage investtigation and assessment and geopark construction projects,some key technologic methods analyzed in this paper are as follows:(1) 展开更多
关键词 GEOHERITAGE investigation and assessment GEOPARK KEY technology EXEMPLIFICATION ANALYSIS
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Experimental Investigation on Finishing Technology by Magnetostrictive Ultrasonic Vibration of Magnetic Liquid
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作者 陈善飞 顾邦明 +1 位作者 王正才 王正良 《Defence Technology(防务技术)》 SCIE EI CAS 2010年第3期166-170,共5页
Magnetic liquid can produce alternative internal pressure under the alternative high-frequency gradient magnetic field.Because it has higher bulk modulus,the internal pressure results in its volume change.Using piezoe... Magnetic liquid can produce alternative internal pressure under the alternative high-frequency gradient magnetic field.Because it has higher bulk modulus,the internal pressure results in its volume change.Using piezoelectric transducers,the ultrasonic wave generated by the vibration of magnetic liquids can be detected,which shows that the magnetic liquids have the magnetostrictive effect and can generate the ultrasonic vibration under the alternative magnetic gradient field.Some nonmagnetic abrasives and rust-proofing agents can be mixed into the magnetic liquids,under the alternative magnetic field,the abrasives held by magnetic liquids grind the surface of the workpieces,and thus,the finishing for the surface with complex shape,mold cavity and inner wall of tiny tubes can be realized. 展开更多
关键词 materials synthesis and processing technology FINISHING magnetic liquid MAGNETOSTRICTIVE ultrasonic vibration
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The status and prospects of in situ grain harvesting technology and its machine system
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作者 Jiang Yiyuan Xu Jiamei Du Chenghai 《Journal of Northeast Agricultural University(English Edition)》 CAS 1994年第1期68-75,共8页
Two main types of in situ grain harvesttng machine are introduced. The comparison of their working principles and advantages are given. The limitations and prospects for stripper header are discussed.
关键词 in situ harvesting technology stripper header threshing prior to cutting
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异质生境下山东银莲花的转录组分析及EST-SSR分子标记开发
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作者 单筱涵 安康 +4 位作者 周春霞 张鑫 逄玉娟 李丽霞 卞福花 《广西植物》 北大核心 2025年第1期95-107,共13页
为探究稀有物种山东银莲花在全光照的山顶灌丛和阴暗的针阔混交林下两种不同生境中的生态适应机制,并开发其EST-SSR分子标记,该研究利用Illumina高通量测序技术对开花期的山东银莲花叶片进行转录组测序,获取其功能注释和差异表达基因(DE... 为探究稀有物种山东银莲花在全光照的山顶灌丛和阴暗的针阔混交林下两种不同生境中的生态适应机制,并开发其EST-SSR分子标记,该研究利用Illumina高通量测序技术对开花期的山东银莲花叶片进行转录组测序,获取其功能注释和差异表达基因(DEGs)。结果表明:(1)转录组测序共得到53536条Unigenes序列,其中27448条成功获得注释。(2)差异表达基因5635个,1600个在山顶灌丛的山东银莲花中上调表达,其余4035个下调表达。有2460个差异表达基因注释到GO数据库2533个三级条目中,1051个差异表达基因注释到KEGG数据库的113条代谢通路中。(3)山东银莲花适应于异质生境的代谢通路主要涉及光合作用-天线蛋白通路和类黄酮生物合成通路,光合作用-天线蛋白通路中lhca 5基因上调表达,lhcb 1、lhcb 2和lhcb 3基因下调表达,类黄酮生物合成通路中chs、c 4 h、f 3′h、f 3 h、fls、ans、chi、ccoaomt和hct基因均上调表达。(4)从山东银莲花转录组数据中共获得7146个SSR位点分布于6006条Unigenes序列中,共计106种重复基序,优势重复基序为单核苷酸重复。设计合成100对EST-SSR引物中共有68对引物具有有效性,其中11对具有多态性,共扩增24个多态性片段。该研究首次开发了山东银莲花EST-SSR分子标记,为山东银莲花的保护和利用提供了重要的分子标记资源。 展开更多
关键词 异质生境 山东银莲花 转录组 est-SSR 生态适应机制
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云南南涧县古茶树资源遗传多样性的EST-SSR分析
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作者 刘娇 杨雄伟 +2 位作者 何胜聪 程小毛 黄晓霞 《热带亚热带植物学报》 北大核心 2025年第3期261-268,共8页
为探究栽培型古茶树阿萨姆茶(Camellia sinensis var. assamica)种质资源的遗传多样性,采用EST-SSR分子标记技术对云南南涧县无量山镇古茶园64份种质资源进行遗传多样性和遗传结构分析。结果表明, 20对引物共检测出223个等位基因,群体... 为探究栽培型古茶树阿萨姆茶(Camellia sinensis var. assamica)种质资源的遗传多样性,采用EST-SSR分子标记技术对云南南涧县无量山镇古茶园64份种质资源进行遗传多样性和遗传结构分析。结果表明, 20对引物共检测出223个等位基因,群体间平均有效等位基因数为3.48个;观测等位基因数(N_(a))为6.25;有效等位基因数(N_(e))为2.983;Shannon多样性指数(I)为1.251;Nei基因多样性指数(H)为0.646。POPGENE分析表明遗传分化系数(F_(st))为0.063,居群间存在中度分化,基因流(N_(m))为3.710。AMOVA分子方差分析表明,阿萨姆茶的遗传变异14%发生在居群间,86%发生在居群内,说明阿萨姆茶居群遗传变异主要发生在居群内部,且基因交流丰富。南涧县古茶园古茶树居群的遗传多样性丰富,这为阿萨姆茶种质资源的保护利用和新品种选育提供了科学依据。 展开更多
关键词 古茶园 古茶树 est-SSR 遗传多样性
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Review of Restoration Technology and Multi-environmental Factors Analysis of West Lake Ecosystem in Hangzhou City
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作者 Wang Xin Zhao Congcong Liu Yang 《Journal of Northeast Agricultural University(English Edition)》 CAS 2022年第3期37-47,共11页
The urban lake ecosystem has the characteristics of long-term,complex and comprehensive in its ecological restoration process because it is related to social and economic activities at multiple levels.Located in Hangz... The urban lake ecosystem has the characteristics of long-term,complex and comprehensive in its ecological restoration process because it is related to social and economic activities at multiple levels.Located in Hangzhou City,China,the West Lake is a national-level scenic spot and a world heritage cultural landscape.It provides key ecosystem services for the city and has a significant impact on the urban ecological environment and the well-being of citizens.The West Lake has undergone an 1800-year governance process in history,realizing the harmonious coexistence with Hangzhou City.Since modern times,due to the rapid development of urbanization and industrialization,the water quality of the West Lake and its freshwater ecosystem have been greatly damaged.Since 2002,Hangzhou City has achieved the overall restoration of the West Lake ecosystem through a series of measures,such as water diversion projects,dredging projects,and the West Lake comprehensive protection project,and the water environment and lakeshore landscape have been greatly improved.This study analyzed the restoration technology of the West Lake ecosystem from two scales of the watershed and the site and discussed the water ecological governance system of the West Lake in the past 20 years.Through thedata analyses of the West Lake land use,vegetation,TP,TN,NH_(3)-N,COD_(Mn) and other multi-environmental factors,the results showed that the water quality,transparency and eutrophication of the West Lake had been greatly improved.At the same time,the application of large-scale restoration methods and technologies also required long-term observation,and attention should be paid to the proliferation of alien species and the loss of heritage value,so as to further avoid potential ecological risks caused by ecological restoration. 展开更多
关键词 urban lake aquatic ecosystem ecological restoration technology comprehensive environmental protection project
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基于转录组序列的龙脑樟EST-SSR标记开发及通用性分析
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作者 黄文丁 胡蓉 +6 位作者 赖猛 杨亮 罗馨 陈婷萱 谢文磊 谢海萍 易敏 《西南林业大学学报(自然科学)》 北大核心 2025年第5期79-86,共8页
利用MISA软件对通过转录组测序获得的187095条龙脑樟unigenes进行SSR位点检测,探讨SSR的分布规律及组成特征并设计引物,进行引物多态性和通用性分析。结果表明:在187095条unigenes中共搜索到90590个SSR位点,SSR出现频率为48.42%,平均1.9... 利用MISA软件对通过转录组测序获得的187095条龙脑樟unigenes进行SSR位点检测,探讨SSR的分布规律及组成特征并设计引物,进行引物多态性和通用性分析。结果表明:在187095条unigenes中共搜索到90590个SSR位点,SSR出现频率为48.42%,平均1.99 kb出现1个SSR位点,含有SSR位点的unigenes共计68573个,SSR发生频率为36.65%。龙脑樟EST-SSR重复类型中除单核苷酸外,二核苷酸重复类型出现频率最高,其次是三核苷酸。在检测到的21777个二核苷酸重复SSR中,重复单元AG/CT(16.06%)数量最多,为优势重复单元,其次是AT/AT(5.37%)、AC/GT(2.53%)、CG/CG(0.09%)。三核苷酸中AAG/CTT(4.37%)出现频率最高,为优势重复单元。利用Primer3.0设计并合成118对SSR引物,有96对扩增成功(81.36%),其中14对呈现多态(11.86%),在5种不同化学类型中的平均多态性信息含量(PIC)为0.381,其中有3对引物属于高多态性位点。开发的14对EST-SSR引物在同属的油樟、牛樟、黄樟、阴香、猴樟等5个物种中的通用性为100%。综上所述,基于龙脑樟转录组开发的EST-SSR标记具有较高的多态性和通用性,该方法是可行的。 展开更多
关键词 龙脑樟 转录组 est-SSR标记 通用性
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THE LAND USE INVESTIGATION IN TIBET PLATEAU USING REMOTE SENSING TECHNOLOGY
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作者 Liu Jiyuan(The Institute of Remote Sensing Application, Academia Sinica, Beijing) 《遥感信息》 CSCD 1990年第A02期5-6,共2页
Tibet Plateau is Known as "the Roof of the World" with the area of 1,220,000km^2, which is about 1/8 land area of China. Because of the high elevation, cold climate and it caused difficulties in regional eco... Tibet Plateau is Known as "the Roof of the World" with the area of 1,220,000km^2, which is about 1/8 land area of China. Because of the high elevation, cold climate and it caused difficulties in regional economic planning and land resources management. Since 1985, the land use investigation in Tibet has been carried out, in which the basic data and thematic maps must be obtained and completed at county and township levels, in order to meet the needs of local administrations. In the investigation, remote sensing technology was comprehensively adopted. At present, the investigation in county level had been completed and the compilation is going to be carried out. Due to paying a great attention to studying on a series of key technical problems, the systematic methods of using remote sensing technology in the plateau land use investigation were formed and successfully put into application. 展开更多
关键词 DATA THE LAND USE INVestIGATION IN TIBET PLATEAU USING REMOTE SENSING technology
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Preparation of spherical HMX@PDA-based PBX by co-axial droplet microfluidic technology:Enhancing the interfacial effect and safety performance of composite microspheres 被引量:1
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作者 Yunyan Guo Yi Liu +6 位作者 Jiani Xie Jiawei Li Fan Wang Jinshan Lei Chongwei An Zhongliang Ma Bidong Wu 《Defence Technology(防务技术)》 2025年第3期73-83,共11页
Surface engineering plays a crucial role in improving the performance of high energy materials,and polydopamine(PDA)is widely used in the field of energetic materials for surface modification and functionalization.In ... Surface engineering plays a crucial role in improving the performance of high energy materials,and polydopamine(PDA)is widely used in the field of energetic materials for surface modification and functionalization.In order to obtain high-quality HMX@PDA-based PBX explosives with high sphericity and a narrow particle size distribution,composite microspheres were prepared using co-axial droplet microfluidic technology.The formation mechanism,thermal behavior,mechanical sensitivity,electrostatic spark sensitivity,compressive strength,and combustion performance of the microspheres were investigated.The results show that PDA can effectively enhance the interfacial interaction between the explosive particles and the binder under the synergistic effect of chemical bonds and the physical"mechanical interlocking"structure.Interface reinforcement causes the thermal decomposition temperature of the sample microspheres to move to a higher temperature,with the sensitivity to impact,friction,and electrostatic sparks(for S-1)increasing by 12.5%,31.3%,and 81.5%respectively,and the compressive strength also increased by 30.7%,effectively enhancing the safety performance of the microspheres.Therefore,this study provides an effective and universal strategy for preparing high-quality functional explosives,and also provides some reference for the safe use of energetic materials in practical applications. 展开更多
关键词 Droplet microfluidic technology Interfacial reinforcement Safety performance Surface modification POLYDOPAMINE HMX
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Advances in the Fabrication of Perovskite Solar Cells by Roll-to-Roll Technology
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作者 ZHAO Jiawei CHEN Haolin +1 位作者 LUO Ni LIU Zhenguo 《材料导报》 北大核心 2025年第1期98-114,共17页
In recent years,perovskite solar cells(PSCs)have garnered significant attention as a potential mainstream technology in the future photovol-taic(PV)market.This is primarily attributed to their salient advantages inclu... In recent years,perovskite solar cells(PSCs)have garnered significant attention as a potential mainstream technology in the future photovol-taic(PV)market.This is primarily attributed to their salient advantages including high efficiency,low cost,and ease of preparation.Nota-bly,the power conversion efficiency(PCE)of PSCs has experienced a remarkable increase from 3.8%in 2009 to over 26%at present.Conse-quently,the adoption of roll-to-roll(R2R)technology for PSCs is considered a crucial step towards their successful commercialization.This arti-de reviews the diverse substrates,scalable deposition techniques(such as solution-based knife-coating and spraying technology),and optimiza.tion procedures employed in recent years to enhance device performance within the R2R process.Additionally,novel perspectives are presented to enrich the existing knowledge in this field. 展开更多
关键词 perovskite solar cells roll-to-roll technology substrate scalable deposition technology performance optimization
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High signal-to-noise ratio microwave signal generation based on SBS assisted optical heterodyne technology
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作者 LUO Qiaoxia ZHAO Zhongbin +3 位作者 HU Tianhao ZHOU Yong ZHENG Ziqi GAO Weiqing 《量子电子学报》 北大核心 2025年第5期677-685,共9页
In this work,the generation of high signal-to-noise ratio(SNR)single-frequency microwave signal without noise sidebands is demonstrated based on the interaction of integrated all-fiber lasers.The microwave signals are... In this work,the generation of high signal-to-noise ratio(SNR)single-frequency microwave signal without noise sidebands is demonstrated based on the interaction of integrated all-fiber lasers.The microwave signals are generated by the interference between a narrow linewidth Brillouin pump light from a single-frequency laser and the Stokes light generated by it.Firstly,the linewidths of the Stokes lights are compressed to~43 Hz based on the stimulated Brillouin scattering(SBS)effect,which ensures that the frequency noise is as low as possible.And then,the relative intensity noise(RIN)of the first order Stokes light is reduced by 21 dB/Hz based on the noise dynamics principle in cascaded SBS effect.By simultaneously reducing the frequency noise and the intensity noise of the coherent signals,the noise sidebands of microwave signals are completely suppressed.As result,the SNR of the microwave signal is improved from 48 dB to 84 dB at the first-order Brillouin frequency shift of 9.415 GHz.Meanwhile,a microwave signal with a SNR of 70 dB is generated at the second-order Brillouin frequency shift of 18.827 GHz.This kind of microwave signals with narrow linewidth and high SNR can provide higher detection resolution and higher transmission efficiency for applications on radar,satellite communication and so on. 展开更多
关键词 laser techniques fiber lasers microwave photonics optical heterodyne technology noise suppression
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冰草高蛋白含量相关EST-SSR标记开发与验证
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作者 杨东升 刘雨雷 +5 位作者 王海伟 苏志芳 王海燕 郭利平 安琪 郝水源 《草业科学》 北大核心 2025年第9期2244-2251,共8页
粗蛋白含量是冰草(Agropyron)重要的营养品质性状之一。为获得与冰草粗蛋白含量紧密相关的EST-SSR标记,本试验以蒙古冰草(A.mongolicum)、航道冰草(A.cristatum)及其杂交加倍后代四倍体冰草F_(2)分离群体为材料,基于群体分离分析法(BSA... 粗蛋白含量是冰草(Agropyron)重要的营养品质性状之一。为获得与冰草粗蛋白含量紧密相关的EST-SSR标记,本试验以蒙古冰草(A.mongolicum)、航道冰草(A.cristatum)及其杂交加倍后代四倍体冰草F_(2)分离群体为材料,基于群体分离分析法(BSA)技术,利用42对EST-SSR引物进行PCR扩增,开发、筛选冰草高蛋白含量相关的ESTSSR标记,并通过F_(2)分离单株进行标记位点验证和相关性分析。结果表明:获得1个与粗蛋白含量相关的分子标记SSR-BC5-82,该标记与粗蛋白含量呈极显著相关关系(P<0.01),准确度高、可靠性强。本研究结果对高效创制四倍体冰草高蛋白新种质及丰富冰草分子标记类型具有重要意义。 展开更多
关键词 冰草 高蛋白含量 est-SSR标记 开发 验证
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Research on the development of electric agricultural machinery technology based on patent information
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作者 LIU Qin HU Lianglong GAO Ya 《智能化农业装备学报(中英文)》 2025年第4期64-72,共9页
This study takes the patent information of China's electric agricultural machinery technology as the research object,and uses patent measurement analysis method and visual analysis tools to carry out statistical a... This study takes the patent information of China's electric agricultural machinery technology as the research object,and uses patent measurement analysis method and visual analysis tools to carry out statistical analysis and comparative research on the relevant patent information from the perspectives of annual patent applications,patent structure,technological key,main applicants,patent layout,technology lifecycle and so on.The research results show that China's electric agricultural machinery technology innovation is developing rapidly,and the patent categories are mainly utility model and invention patents,and the proportion of invention patents is lower than utility model patents.Universities are the most important innovation subjects in this field,and technology research and development focuses on IPC(international patent classification)subcategories such as A01C(seeding,fertilization),B60K(power,transmission),and A01D(harvesting.mowing).Jiangsu Province and Shandong Province rank the top 2 in the number of domestic patent applications,and the United States and Japan are the two countries with the most layout in China.At present,the growth of electric agricultural machinery technology is weak,but the new technology has strong features,there is no sign of maturity,and it does not have signs of technological aging and still has greater development potential.The results of the study can provide information reference for clarifying the status quo,characteristics and development trend of China's electric agricultural machinery technology innovation. 展开更多
关键词 China electric agricultural machinery technology development patent analysis technology lifecycle IPC
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Preparation of HMX-based energetic microspheres with efficient self-healing function by microchannel technology to enhance storage performance and interface bonding effect
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作者 Wenqing Li Mianji Qiu +5 位作者 Wangjian Cheng Qian Yang Xiaohong Yan Yousheng Qiu Chongwei An Baoyun Ye 《Defence Technology(防务技术)》 2025年第10期47-59,共13页
The self-healing function is considered one of the effective ways to address structural damage and improve interfacial bonding in Energetic composite materials(ECMs).However,the currently prepared ECMs with self-heali... The self-healing function is considered one of the effective ways to address structural damage and improve interfacial bonding in Energetic composite materials(ECMs).However,the currently prepared ECMs with self-healing function have problems such as irregular particle shape and uneven distribution of components,which affect the efficient play of self-healing function.In this paper,HMX-based energetic microspheres with self-healing function were successfully prepared by microchannel technology,which showed excellent self-healing effect in both Polymer-bonded explosives(PBXs)and Composite solid propellants(CSPs).The experimental results show that the HMX-based energetic microspheres with different binder contents prepared by microchannel technology show regular shape,HMX crystal particles are uniformly wrapped by self-healing binder(GAPU).When the content of GAPU in HMX-based energetic microspheres is 10%,PBXs show excellent self-healing effect and mechanical safety is improved by 400%(raw HMX vs S4,5 J vs 25 J).As a high-energy component,the burning rate of CSPs is increased by 359.4%,the time(burning temperature>1700℃)is prolonged by 333.3%,and the maximum impulse force is increased by 107.3%(CSP-H vs CSP-S4,0.84 mm/s vs 3.87 mm/s,0.06 s vs 0.26 s,0.82 m N vs 1.70 m N).It also has excellent storage performance.The preparation of HMX-based energetic microspheres with self-healing function by microchannel technology provides a new strategy to improve the storage performance of ECMs and the combustion performance of CSPs. 展开更多
关键词 SELF-HEALING HMX Microchannel technology Storage performance Combustion performance
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基于转录组测序的侧柏EST-SSR引物开发与鉴定分析
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作者 李锦秋 毕楷杰 +3 位作者 王立成 柳俊明 李清泉 顾丽姣 《林业与生态科学》 2025年第3期239-246,共8页
为了评价和利用侧柏种质资源,基于转录组测序技术,以侧柏的根、茎、叶为材料开发EST-SSR引物,并以黄叶侧柏MC1的半同胞子代黄叶苗和绿叶苗为样本,评估引物在不同侧柏资源中的适用性和多态性。结果表明根器官转录组中SSR位点数大于茎器... 为了评价和利用侧柏种质资源,基于转录组测序技术,以侧柏的根、茎、叶为材料开发EST-SSR引物,并以黄叶侧柏MC1的半同胞子代黄叶苗和绿叶苗为样本,评估引物在不同侧柏资源中的适用性和多态性。结果表明根器官转录组中SSR位点数大于茎器官、叶器官。叶器官中SSR重复类型大于茎器官、根器官。3个器官中优势基序均为A/T、AG/CT、AAG/CTT。3个器官中10、15、18 bp长度的SSR数量最多。60对EST-SSR引物有效率为100%,多态性率为16.67%,不同引物PIC值的变化范围为0.322~0.722。利用10对多态性引物扩增产物数据将样本进行聚类分析和遗传分析,并推测MC1半同胞子代有50%属于异交子代,其余疑似为自交子代,在遗传距离为200时,异交子代群体和疑似自交子代群体被划分为13大类群。 展开更多
关键词 黄叶侧柏MC1 转录组 est-SSR 子代鉴定
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ABSTRACTS SOME SUGGESTIONS OF IMPROVING WELL CONTROL TECHNOLOGY LEVEL
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作者 WANG Zhongsheng LIN Ancun SUN Kai LI Qian PAN Renjie 《钻采工艺》 CAS 北大核心 2009年第3期I0001-I0006,共6页
关键词 石油 钻采工艺 摘要 油气地质
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油茶种子EST文库构建及主要表达基因的分析 被引量:59
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作者 谭晓风 胡芳名 +3 位作者 谢禄山 石明旺 张党权 乌云塔娜 《林业科学》 EI CAS CSCD 北大核心 2006年第1期43-48,共6页
为研究油茶种子在油脂转化高峰期的基因表达并分离克隆与油脂合成等有关的重要基因,以油茶优良无性系湘林1号和湘林4号近成熟种子为材料构建cDNA文库;随机挑取2327个克隆进行3′端测序构建EST文库。将数据完整并无X、N的1979条cDNA序列... 为研究油茶种子在油脂转化高峰期的基因表达并分离克隆与油脂合成等有关的重要基因,以油茶优良无性系湘林1号和湘林4号近成熟种子为材料构建cDNA文库;随机挑取2327个克隆进行3′端测序构建EST文库。将数据完整并无X、N的1979条cDNA序列与NCBI核酸数据库的非冗余序列进行同源性比较,确认763条cDNA序列与核酸数据库中其他物种的DNA序列具有很高或较高的同源性,可确认266种基因;有1216个克隆序列为未知功能的基因序列。各类基因表现出不同的表达丰度,其中贮藏蛋白基因、与基因表达调控相关的基因、抗逆相关基因、种子成熟和胚胎发育相关的基因等为高丰度表达,与脂肪酸代谢相关的基因等为中丰度表达,另外还有大量的其他基因和未知功能基因在种子中表达。各类基因表达的数量和趋势与种子接近发育成熟阶段相吻合。 展开更多
关键词 油茶 CDNA est 文库 表达基因
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