Tea （Camellia sinensis L.） represents different ploidy levels. In the present paper, we reviewed the recent data on the diploid, aneuploid and polyploid formed their origin and chemically induced polyploidy and pred...Tea （Camellia sinensis L.） represents different ploidy levels. In the present paper, we reviewed the recent data on the diploid, aneuploid and polyploid formed their origin and chemically induced polyploidy and predicted it role in teagenetic improvement for better yield. Different polidy levels had different effects on tea physiology. Tetraploid and triploid had more vigour and hardness due to increased size of cells, while triploid could have even more vigours due to increased size of cells and sterilities. Chemically induced polyploidy had been found an important tool for improving plant physiology and production, therefore, induced polyploids should be produced to overcome the problem of low yield and limited rainfall in tea growing areas.展开更多
The research screening of adoptive elite tea clones was conducted at NTRI, Mansehra during 2011-2012. Nine clones 101Aa, 105aa, 108aa, 561aa, ll7aa, 219ab, 470bb and 180bd were evaluated for seedling performance. Rand...The research screening of adoptive elite tea clones was conducted at NTRI, Mansehra during 2011-2012. Nine clones 101Aa, 105aa, 108aa, 561aa, ll7aa, 219ab, 470bb and 180bd were evaluated for seedling performance. Randomized complete block design was used with three replications. Data was recorded on various morphological characters after 8 months. The results showed that high survival percentage, shoot length, number of roots plant-1, number of leaves plant1 and root length were observed in clone 105aa. While the highest fresh weight and dry weight of leaves were observed in clones 117aa and 105aa. The clone 105aa was drought resistant, high survival percentage and root growth. On the basis of the results, clone 105aa was recommended for cultivation through cuttings in the hilly areas of Pakistan where unequal rainfall distribution was a major hitch.展开更多
This study was carried out to obtain useful information and to search quality related components for breeding of tea by analysis of Korean local tea leaves at Sulloc-Cha R&D center in Jeju Island,Korea.It was also...This study was carried out to obtain useful information and to search quality related components for breeding of tea by analysis of Korean local tea leaves at Sulloc-Cha R&D center in Jeju Island,Korea.It was also conducted to measure the major agronomic characteristics of 300 local germplasms.These results allowed us to select some superior lines as genetic resources for green-tea breeding and research genotypes.展开更多
Terpenoids play important roles in productivity and product quality in tea(Camellia sinensis),not only because volatile terpenoids contribute significantly to the aroma of teas,but terpenoid-derived light harvesting p...Terpenoids play important roles in productivity and product quality in tea(Camellia sinensis),not only because volatile terpenoids contribute significantly to the aroma of teas,but terpenoid-derived light harvesting pigments and phytohormones such as strigolactones implement photosynthesis and branching in plants.All terpenoids are derived from isopentenyl diphosphate(IPP),dimethylallyl diphosphate(DMAPP) produced by methyl-erythritol-phosphate(MEP) and mevalonate(MVA) pathways,the early steps of isoprenoid biosynthesis.For the purpose to determine key genes in the MEP and MVA pathways in C.sinensis,five genes with complete coding sequence and three with partial sequence were obtained based on previously attained transcriptome data and polymerase chain reaction approach in this study.In silico analyses suggested that the five genes with full coding sequence individually encoded acetyl-CoA C-acetyltransferase(CsAACT),3-hydroxy-3-methylglutaryl-CoA synthase(CsHMGS),4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase(CsCMK),1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase(CsHDS),and farnesyl pyrophosphate synthase(CsFPS).Additionally,studies on metabolite-mediated gene expression were performed using tea cell suspensions and leaf-disc cultures.Our data showed that the addition of acetyl-CoA increased the transcript level of CsAACT and CsHMGS by 1.5- and 3-fold,respectively.The addition of deoxy-D-xylulose 5-phosphate(DXP) induced the expression of CsCMK and CsHDS by 2.3- and 1.5-fold,respectively.The CsFPS expression was approximately 2-fold higher when treated with DXP,IPP or DMAPP than the untreated control,but this induction was not observed with the acetyl-CoA treatment.Our studies indicated that expression of CsAACT,CsHMGS,CsCMK,CsHDS,and CsFPS were metabolite-mediated in a different extent.展开更多
近年来全球极端低温天气频发,严重影响了茶树的产量和品质。ICE(Inducer of CBF expression)基因家族主要参与植物的低温胁迫响应,但在茶树领域中的相关研究还不够全面。本研究从茶树基因组中鉴定出51个茶树CsICEs基因,对其理化性质、...近年来全球极端低温天气频发,严重影响了茶树的产量和品质。ICE(Inducer of CBF expression)基因家族主要参与植物的低温胁迫响应,但在茶树领域中的相关研究还不够全面。本研究从茶树基因组中鉴定出51个茶树CsICEs基因,对其理化性质、基因结构和启动子顺式作用元件展开生物信息学分析。茶树CsICEs基因的启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件,其可能参与多种逆境胁迫响应。转录组分析和RT-qPCR验证结果发现,低温下CsICE43基因的表达量上升了4.24倍,其可能与茶树低温响应相关。以茶树品种‘保靖黄金茶1号’的cDNA为模板,克隆获得了CsICE43基因,其在不同组织中的表达模式存在差异,在顶芽和嫩叶中特异性高表达。蛋白氨基酸序列和系统进化树分析表明,CsICE43基因包含与ICE家族其他成员一致的S-rich、bHLH、ACT等保守结构域,且与毛花猕猴桃(Actinidiaeriantha)的亲缘关系较近。在STRING在线网站中以拟南芥AtICEs为模型,推测茶树CsICE43蛋白与HOS1、MYB15、DREB1/2存在潜在的互作关系。亚细胞定位试验表明CsICE43定位于细胞核,与跨膜结构分析结果一致。综上所述,本研究发现CsICE43基因可能与茶树低温响应关联,为深入挖掘其基因功能与抗寒分子机理提供了一定的理论基础。展开更多
文摘Tea （Camellia sinensis L.） represents different ploidy levels. In the present paper, we reviewed the recent data on the diploid, aneuploid and polyploid formed their origin and chemically induced polyploidy and predicted it role in teagenetic improvement for better yield. Different polidy levels had different effects on tea physiology. Tetraploid and triploid had more vigour and hardness due to increased size of cells, while triploid could have even more vigours due to increased size of cells and sterilities. Chemically induced polyploidy had been found an important tool for improving plant physiology and production, therefore, induced polyploids should be produced to overcome the problem of low yield and limited rainfall in tea growing areas.
文摘The research screening of adoptive elite tea clones was conducted at NTRI, Mansehra during 2011-2012. Nine clones 101Aa, 105aa, 108aa, 561aa, ll7aa, 219ab, 470bb and 180bd were evaluated for seedling performance. Randomized complete block design was used with three replications. Data was recorded on various morphological characters after 8 months. The results showed that high survival percentage, shoot length, number of roots plant-1, number of leaves plant1 and root length were observed in clone 105aa. While the highest fresh weight and dry weight of leaves were observed in clones 117aa and 105aa. The clone 105aa was drought resistant, high survival percentage and root growth. On the basis of the results, clone 105aa was recommended for cultivation through cuttings in the hilly areas of Pakistan where unequal rainfall distribution was a major hitch.
文摘This study was carried out to obtain useful information and to search quality related components for breeding of tea by analysis of Korean local tea leaves at Sulloc-Cha R&D center in Jeju Island,Korea.It was also conducted to measure the major agronomic characteristics of 300 local germplasms.These results allowed us to select some superior lines as genetic resources for green-tea breeding and research genotypes.
基金funded by the National Science Foundation in China (#31070614 to S.Wei)Fund for the Doctoral Program of Higher Education of The ministry of Education(#20123418110002,to S.Wei)+1 种基金the Program for Changjiang Scholars and Innovative Research Team in Universities (IRT1101 to Z.Z.Zhang)the "Twelfth Five-Year" National Key Basic Research and Development Project (973) in China (2012CB722903 to Xiao-Chun Wan)
文摘Terpenoids play important roles in productivity and product quality in tea(Camellia sinensis),not only because volatile terpenoids contribute significantly to the aroma of teas,but terpenoid-derived light harvesting pigments and phytohormones such as strigolactones implement photosynthesis and branching in plants.All terpenoids are derived from isopentenyl diphosphate(IPP),dimethylallyl diphosphate(DMAPP) produced by methyl-erythritol-phosphate(MEP) and mevalonate(MVA) pathways,the early steps of isoprenoid biosynthesis.For the purpose to determine key genes in the MEP and MVA pathways in C.sinensis,five genes with complete coding sequence and three with partial sequence were obtained based on previously attained transcriptome data and polymerase chain reaction approach in this study.In silico analyses suggested that the five genes with full coding sequence individually encoded acetyl-CoA C-acetyltransferase(CsAACT),3-hydroxy-3-methylglutaryl-CoA synthase(CsHMGS),4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase(CsCMK),1-hydroxy-2-methyl-2-(E)-butenyl-4-diphosphate synthase(CsHDS),and farnesyl pyrophosphate synthase(CsFPS).Additionally,studies on metabolite-mediated gene expression were performed using tea cell suspensions and leaf-disc cultures.Our data showed that the addition of acetyl-CoA increased the transcript level of CsAACT and CsHMGS by 1.5- and 3-fold,respectively.The addition of deoxy-D-xylulose 5-phosphate(DXP) induced the expression of CsCMK and CsHDS by 2.3- and 1.5-fold,respectively.The CsFPS expression was approximately 2-fold higher when treated with DXP,IPP or DMAPP than the untreated control,but this induction was not observed with the acetyl-CoA treatment.Our studies indicated that expression of CsAACT,CsHMGS,CsCMK,CsHDS,and CsFPS were metabolite-mediated in a different extent.
文摘近年来全球极端低温天气频发,严重影响了茶树的产量和品质。ICE(Inducer of CBF expression)基因家族主要参与植物的低温胁迫响应,但在茶树领域中的相关研究还不够全面。本研究从茶树基因组中鉴定出51个茶树CsICEs基因,对其理化性质、基因结构和启动子顺式作用元件展开生物信息学分析。茶树CsICEs基因的启动子区域富含光响应、植物激素、生长发育及非生物胁迫相关顺式作用元件,其可能参与多种逆境胁迫响应。转录组分析和RT-qPCR验证结果发现,低温下CsICE43基因的表达量上升了4.24倍,其可能与茶树低温响应相关。以茶树品种‘保靖黄金茶1号’的cDNA为模板,克隆获得了CsICE43基因,其在不同组织中的表达模式存在差异,在顶芽和嫩叶中特异性高表达。蛋白氨基酸序列和系统进化树分析表明,CsICE43基因包含与ICE家族其他成员一致的S-rich、bHLH、ACT等保守结构域,且与毛花猕猴桃(Actinidiaeriantha)的亲缘关系较近。在STRING在线网站中以拟南芥AtICEs为模型,推测茶树CsICE43蛋白与HOS1、MYB15、DREB1/2存在潜在的互作关系。亚细胞定位试验表明CsICE43定位于细胞核,与跨膜结构分析结果一致。综上所述,本研究发现CsICE43基因可能与茶树低温响应关联,为深入挖掘其基因功能与抗寒分子机理提供了一定的理论基础。
