采用ISSR标记方法,对南方红豆杉〔Taxus chinensis(Pilger)Rehd.var.mairei(Lemée et Lévl.)Cheng et L.K.Fu〕3个野生种群(包括江西黄港和黄沙种群以及福建枫溪种群)、2个迁地保护栽培种群及2个迁地保护衍生种群(均位于江苏...采用ISSR标记方法,对南方红豆杉〔Taxus chinensis(Pilger)Rehd.var.mairei(Lemée et Lévl.)Cheng et L.K.Fu〕3个野生种群(包括江西黄港和黄沙种群以及福建枫溪种群)、2个迁地保护栽培种群及2个迁地保护衍生种群(均位于江苏南京中山植物园和江西庐山植物园)的遗传多样性和遗传结构进行了分析和比较。结果显示:用8个引物从南方红豆杉的基因组总DNA中共扩增出73条带,其中多态性条带62条。2个迁地保护衍生种群的多态性条带百分率(PPB)、Nei’s多样性指数(h)和Shannon信息指数(I)较高,3个野生种群的PPB、h和I值总体上居中,而2个迁地保护栽培种群的PPB、h和I值较低。合并后的迁地保护衍生种群以及野生种群均有较高的遗传多样性,二者的PPB、h和I值相近,分别为78.08%和82.19%、0.207 6和0.205 8、0.322 9和0.325 9;但二者的遗传结构存在差异,迁地保护衍生种群的遗传分化系数(GST)为0.068 9,明显低于野生种群(0.168 5)。合并后的迁地保护栽培种群的遗传多样性相对较低,PPB、h和I值分别为60.27%、0.180 7和0.275 2;而GST值最高(0.251 4),明显高于合并后的野生种群和迁地保护衍生种群。研究结果表明:在植物园次生林环境条件下,回归到自然生境下的南方红豆杉迁地保护衍生种群的遗传多样性趋于丰富并接近野生种群,从而证明了植物园在濒危植物迁地保护中具有以往未被认识到的巨大潜力。展开更多
报道了红豆杉属1新变型——保康红豆杉[Taxus chinensis(Pilger)Rehd f.baokangsis Y.J.Fei]。该变型与原变种南方红豆杉[Taxus.chinensis(Pilger)Rehd.var.mairei(Lemee et Lévl.)Cheng et L.K.Fu]主要区别在于其雄株上有部分枝...报道了红豆杉属1新变型——保康红豆杉[Taxus chinensis(Pilger)Rehd f.baokangsis Y.J.Fei]。该变型与原变种南方红豆杉[Taxus.chinensis(Pilger)Rehd.var.mairei(Lemee et Lévl.)Cheng et L.K.Fu]主要区别在于其雄株上有部分枝条发育成雌性枝条或雌雄球花在同一果枝上,呈现雌雄同株现象。凭证模式标本藏于武汉植物园标本馆(编号Y.J.Fei001,HIB)。展开更多
The seed of \%Taxus chinensis \%(Pilger) Rehd.\ has a long postmature duration only with the germination of 29.5%～37.5% after 40～70 d when its dormancy is relieved. The low limit of mean temperature for shooting is...The seed of \%Taxus chinensis \%(Pilger) Rehd.\ has a long postmature duration only with the germination of 29.5%～37.5% after 40～70 d when its dormancy is relieved. The low limit of mean temperature for shooting is 15℃, the mean height of 1\|year\|old and 2\|years\|old seedlings is 8.12 cm and 16.10 cm respectively, mean basal diameter of 2\|years\|old seedling is 0.19 cm. Soft\|wood + 50 mg/kg ABT + sandy bed in spring and hard\|wood + 100 mg/kg NAA + sandy bed in autumn are the best treatments for cutting propagation, the survival rate of afforestation may reach 100%. Their mean height is 70.48 cm, basal diameter is 1.01 cm, and branch is 40～50 cm in second year for plantation.展开更多
The embryo culture of Taxus chinensis (Pilger) Rehd. and T. brevifolia Nutt. in vitro can shorten the dormancy period with early germinating, germination percentage is above 98% after cultured thirty days. The surviva...The embryo culture of Taxus chinensis (Pilger) Rehd. and T. brevifolia Nutt. in vitro can shorten the dormancy period with early germinating, germination percentage is above 98% after cultured thirty days. The survival rate of the seedlings increased when the seeds were stored in low temperature, it could be increased from 49% to 70% after treated four months. The seedling′s growth can be improved if reduce the main inorganic element content of the medium. Germination character of two \%Taxus \%L. species are similar.展开更多
Taxol content from the branches and leaves of Taxus chinensis var. mairei (Lemeé et Lévl.) Cheng et L. K. Fu and its cell cultures was analyzed by using Nova-Pak C-{18}column and improving the proportion of ...Taxol content from the branches and leaves of Taxus chinensis var. mairei (Lemeé et Lévl.) Cheng et L. K. Fu and its cell cultures was analyzed by using Nova-Pak C-{18}column and improving the proportion of mobile phase (methanol/acetonitrile/water) and modifing column temperature at a wavelength of 227 nm. The improved HPLC method is celerity, nicety and delicacy. The lowest detectable quantity of taxol could reach 0.005 μg.展开更多
文摘报道了红豆杉属1新变型——保康红豆杉[Taxus chinensis(Pilger)Rehd f.baokangsis Y.J.Fei]。该变型与原变种南方红豆杉[Taxus.chinensis(Pilger)Rehd.var.mairei(Lemee et Lévl.)Cheng et L.K.Fu]主要区别在于其雄株上有部分枝条发育成雌性枝条或雌雄球花在同一果枝上,呈现雌雄同株现象。凭证模式标本藏于武汉植物园标本馆(编号Y.J.Fei001,HIB)。
文摘The seed of \%Taxus chinensis \%(Pilger) Rehd.\ has a long postmature duration only with the germination of 29.5%～37.5% after 40～70 d when its dormancy is relieved. The low limit of mean temperature for shooting is 15℃, the mean height of 1\|year\|old and 2\|years\|old seedlings is 8.12 cm and 16.10 cm respectively, mean basal diameter of 2\|years\|old seedling is 0.19 cm. Soft\|wood + 50 mg/kg ABT + sandy bed in spring and hard\|wood + 100 mg/kg NAA + sandy bed in autumn are the best treatments for cutting propagation, the survival rate of afforestation may reach 100%. Their mean height is 70.48 cm, basal diameter is 1.01 cm, and branch is 40～50 cm in second year for plantation.
文摘The embryo culture of Taxus chinensis (Pilger) Rehd. and T. brevifolia Nutt. in vitro can shorten the dormancy period with early germinating, germination percentage is above 98% after cultured thirty days. The survival rate of the seedlings increased when the seeds were stored in low temperature, it could be increased from 49% to 70% after treated four months. The seedling′s growth can be improved if reduce the main inorganic element content of the medium. Germination character of two \%Taxus \%L. species are similar.
文摘Taxol content from the branches and leaves of Taxus chinensis var. mairei (Lemeé et Lévl.) Cheng et L. K. Fu and its cell cultures was analyzed by using Nova-Pak C-{18}column and improving the proportion of mobile phase (methanol/acetonitrile/water) and modifing column temperature at a wavelength of 227 nm. The improved HPLC method is celerity, nicety and delicacy. The lowest detectable quantity of taxol could reach 0.005 μg.
