A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic character...A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic characteristics were investigated. All of the isolates were identified as A. hydrophila by traditional biochemical method and yielded a 686-bp DNA fragment of the 16S rDNA gene in the PCR experiments. Collected strains were also evaluated for their susceptibility to 17 different antibiotics. The isolates showed an even trend of the resistance and sensitivity to drugs, highly sensitive to antibiotics, such as Levofloxacin, PolymyxinB, Ofloxacin and resistant to antibiotics, such as Bristopen, Lincomycin, Ampicillin, Teicoplanin. Evaluation of genetic diversity was performed on all isolates by molecular typing with enterobacterial repetitive intergenic consensus （ERIC-PCR） method. The results showed that three different types, i.e. type I, ]I, and type ~I, were found in 59 isolates and type III accounted for a large proportion of 54.84%. There was no dominant difference between the tendency of the isolates of Heilongjiang Province and Jilin Province in these three types, which showed DI 〉 I 〉 I, while the isolates of Liaoning Province showed III 〉 II 〉 I. The percentage of different types in different provinces varied in each other; however, they didn＇t show any obvious regional or cluster-specific branches. In conclusion, the ability to distinguish Aeromonas hydrophila strains from diseased common carp with ERIC-PCR would be useful for epidemiological investigation and population genetic analysis of this pathogen in China.展开更多
Establishing Consensus with Users of Research Irradiator Devices to Facilitate Source Type Replacement Danette R.Fennesy1,Janet M.Gutiérrez1,2,Scott J.Patlovich1,Robert J.Emery1(1.The University of Texas Health S...Establishing Consensus with Users of Research Irradiator Devices to Facilitate Source Type Replacement Danette R.Fennesy1,Janet M.Gutiérrez1,2,Scott J.Patlovich1,Robert J.Emery1(1.The University of Texas Health Science Center at Houston,Environmental Health&Safety,6431 Fannin St,CYF G.102,Houston,TX,77030;2.Corresponding author)Abstract:The ability to irradiate cells,tissues,and other biological materials with high-energy photons has been an essential tool in the discovery of numerous biomedical research advancements.展开更多
An evolution inequality of Sobolev type involving a nonlinear convolution term is considered.By using the nonlinear capacity method and the contradiction argument,the non-existence of the nontrivial local weak solutio...An evolution inequality of Sobolev type involving a nonlinear convolution term is considered.By using the nonlinear capacity method and the contradiction argument,the non-existence of the nontrivial local weak solution is proved.展开更多
1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated fro...1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.展开更多
2025年6月16日,上海交通大学医学院附属第一人民医院王宏林团队在Immunity杂志在线发表了题为“Nociceptor-derived CGRP enhances dermal type I conventional dendritic cell function to drive autoreactive CD8+T cell responses in...2025年6月16日,上海交通大学医学院附属第一人民医院王宏林团队在Immunity杂志在线发表了题为“Nociceptor-derived CGRP enhances dermal type I conventional dendritic cell function to drive autoreactive CD8+T cell responses in vitiligo”的研究论文。该研究联合单细胞测序、空间转录组测序、皮肤全包埋透明化成像、近10种基因工程小鼠与研究者发起临床试验(investigator-initiated trial,IIT),揭示了白癜风中存在“感觉神经元-CGRP-cDC1-CD8^(+)T细胞”致病新机制;通过CGRP受体拮抗剂(rimegepant)阻断该神经-免疫互作轴,显著抑制了白癜风模型小鼠的疾病进展,并在57例白癜风患者的临床试验中展现出良好的治疗效果。该研究为白癜风治疗策略研发提供了新的理论依据和靶标,并形成了完整的“临床-基础-临床”的研究环路。展开更多
In this paper,by utilizing the Marcinkiewicz-Zygmund inequality and Rosenthal-type inequality of negatively superadditive dependent(NSD)random arrays and truncated method,we investigate the complete f-moment convergen...In this paper,by utilizing the Marcinkiewicz-Zygmund inequality and Rosenthal-type inequality of negatively superadditive dependent(NSD)random arrays and truncated method,we investigate the complete f-moment convergence of NSD random variables.We establish and improve a general result on the complete f-moment convergence for Sung’s type randomly weighted sums of NSD random variables under some general assumptions.As an application,we show the complete consistency for the randomly weighted estimator in a nonparametric regression model based on NSD errors.展开更多
Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by ...Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by the tests of rock masses subjected to explosion loads to examine its performance.The crack levels of rock mass induced by water-coupled charge blasting and air-coupled charge blasting are first compared.It is found that water-coupled charge blasting is more appropriate to fracture deep rock mass than air-coupled charge blasting.In addition,the effects of rock properties,water-coupled charge coefficients,and borehole connection angles on the performance of water-coupled charge blasting are investigated.The results show that rock properties and water-coupled charge coefficients can greatly influence the crack and fragmentation levels of rock mass induced by water-coupled charge blasting under uniform and non-uniform in-situ stresses.However,changing borehole-connection angles can only affect crack and fragmentation levels of rock mass under non-uniform in-situ stresses but barely affect those under uniform in-situ stresses.A formula is finally proposed by considering the above-mentioned factors to provide the design suggestion of water-coupled charge blasting to fracture rock mass with different in-situ stresses.展开更多
Different solvothermal reactions of ZnC2O_(4)with oxalic acid(H_(2)ox)and 1,2,4-triazole(Htrz)successfully gave a new quaternary(NJTU-Bai83,NJTU-Bai=Nanjing Tech University Bai's group)and a new quinary(NJTU-Bai84...Different solvothermal reactions of ZnC2O_(4)with oxalic acid(H_(2)ox)and 1,2,4-triazole(Htrz)successfully gave a new quaternary(NJTU-Bai83,NJTU-Bai=Nanjing Tech University Bai's group)and a new quinary(NJTU-Bai84)anionic metal-organic frameworks(MOFs),where NJTU-Bai83=(Me_(2)NH_(2))2[Zn_(3)(trz)_(2)(ox)_(3)]·2H_(2)O and NJTU-Bai84=(Me_(2)NH_(2))[Zn_(3)(trz)_(3)(ox)_(2)]·H_(2)O,respectively.With the[Zn_(2)(ox)4(trz)_(2)]secondary building unit(SBU)in NJTU-Bai83 replaced by the[Zn_(3)(ox)_(2)(trz)_(6)]and planar[Zn(ox)_(2)(trz)_(2)]ones in NJTU-Bai84,2D supramolecular building layers(SBLs)are changed from the A-layer and B-layer to another A-layer,while pillars are transformed from the tetrahedral[Zn(ox)_(2)(trz)_(2)]SBU to the irregular tetrahedral[Zn(ox)_(2)(trz)_(2)]and planar[Zn(ox)_(2)(trz)_(2)]SBUs.Thus,cdq-topological quaternary NJTU-Bai83 is tuned to(4,4,8)-c new topological quinary NJTU-Bai84.Two MOFs were well characterized by powder X-ray diffraction,thermogravimetric analysis,elemental analysis,etc.CCDC:2351819,NJTU-Bai83;2351820,NJTU-Bai84.展开更多
目的探讨手机微信管理模式对初发老年2型糖尿病患者健康管理的效果。方法选取2014年1—2月在江西省人民医院内分泌科门诊就诊筛查出的老年初发2型糖尿病患者80例,根据随机数字表法分为试验组和对照组,每组40例。对照组采用门诊常规管理...目的探讨手机微信管理模式对初发老年2型糖尿病患者健康管理的效果。方法选取2014年1—2月在江西省人民医院内分泌科门诊就诊筛查出的老年初发2型糖尿病患者80例,根据随机数字表法分为试验组和对照组,每组40例。对照组采用门诊常规管理模式,试验组采用手机微信管理模式,均干预6个月。比较两组入组及干预后3、6个月时躯体健康监测指标和糖化血红蛋白(Hb A1c)、血压、低密度脂蛋白胆固醇(LDL-C)达标率及低血糖发生率、糖尿病控制状况。结果两组性别、年龄、文化程度、婚姻状况及高血压、高脂血症发生率间差异均无统计学意义(P>0.05)。两组空腹血糖(FPG)、Hb A1c、BMI间差异无统计学意义(P>0.05),不同时间点FPG、Hb A1c、BMI间差异有统计学意义(P<0.05),干预方法和时间在FPG、Hb A1c、BMI上存在交互作用(P<0.05);两组餐后2h血糖(2 h PG)、收缩压、舒张压、总胆固醇(TC)、三酰甘油(TG)、LDL-C间差异有统计学意义(P<0.05),不同时间点2 h PG、收缩压、舒张压、TC、TG、LDL-C间差异有统计学意义(P<0.05),干预方法和时间在2 h PG、收缩压、舒张压、TC、TG、LDL-C上存在交互作用(P<0.05)。两组干预前低血糖发生率均为0,两组干预前Hb A1c、血压、LDL-C达标率间差异无统计学意义(P>0.05);两组干预后3个月Hb A1c、血压达标率及低血糖发生率间差异无统计学意义(P>0.05),而LDL-C达标率间差异有统计学意义(P<0.05);两组干预后6个月Hb A1c、LDL-C达标率间差异有统计学意义(P<0.05),而血压达标率及低血糖发生率间差异无统计学意义(P>0.05)。两组糖尿病及并发症自觉症状得分间差异无统计学意义(P>0.05),不同时间点糖尿病及并发症自觉症状得分间差异有统计学意义(P<0.05),干预方法和时间在糖尿病及并发症自觉症状得分上存在交互作用(P<0.05);两组生活习惯、治疗情况、生存技能、治疗目标、知识结构得分间差异有统计学意义(P<0.05),不同时间点生活习惯、治疗情况、生存技能、治疗目标、知识结构得分间差异有统计学意义(P<0.05),干预方法和时间在生活习惯、治疗情况、生存技能、治疗目标、知识结构得分上存在交互作用(P<0.05)。结论手机微信管理模式对初发老年2型糖尿病患者健康管理效果较好,值得推广。展开更多
A pair of specific primers were designed and synthesized according to the published sequences of ORF1 gene of PCV 2. The complete DNA fragment of ORF1 gene was obtained by PCR from viral DNA of PCV 2 QD strain.Its nuc...A pair of specific primers were designed and synthesized according to the published sequences of ORF1 gene of PCV 2. The complete DNA fragment of ORF1 gene was obtained by PCR from viral DNA of PCV 2 QD strain.Its nuclectide sequence was determined by the dideoxy mediated chain termination method.The results showed that the complete open reading frame (ORF) of ORF1 gene encoding 314 amino acids was 945 bp in length.A comparison of the nucleotide and amino acid sequences of ORF1 gene with that of other PCV strains showed that the identity of nucleotide with PCV 1 and PCV 2 were 83% and 96 4%～99 2% respectively,and identity of the deduced amino acid with PCV 1 and PCV 2 were 84% and more than 98% respecitively.The DNA fragment of ORF1 gene was subcloned into prokaryotic expression vector pET 28a and pGEX KG;while the specific non fusion and fusion proteins with GST of molecular weight 38 kD and 63 kD were expressed in E.coli BL 21 (DE3).Western blotting assay indicated that the polyclonal antibody against PCV 2 could recognize these two proteins.展开更多
云南猝死综合征（Yunnan sudden death syndrome，YSDS）是一种每年6～9月份发生在云南省中部和西北部海拔1815～2225 m的山区、半山区，以突然发病并死亡为特征且病因不明的疾病。该病以青壮年为主，女性多于男性，傈僳族、彝族和苗族...云南猝死综合征（Yunnan sudden death syndrome，YSDS）是一种每年6～9月份发生在云南省中部和西北部海拔1815～2225 m的山区、半山区，以突然发病并死亡为特征且病因不明的疾病。该病以青壮年为主，女性多于男性，傈僳族、彝族和苗族发病率较高，具有家庭聚集性，临床表现多样，病理学特点缺乏特异性，发病原因与卫生、经济条件、硒铬微量元素缺乏、柯萨奇B组病毒感染、食用蘑菇及当地特殊地质条件相关。本文综述了YSDS的流行病学特征、临床表现、病理性特点、病因研究及假说，以期对YSDS进一步研究提供线索。展开更多
目的:对部分D表型孕妇进行免疫血清学和RHD基因型分析。方法:采用常规血型血清学方法鉴定孕妇RhD血型,并进行血型特异性抗体筛查和鉴定;采用序列特异性引物聚合酶链反应(polymerase chain reactionsequence specific primer,PCR-SSP)鉴...目的:对部分D表型孕妇进行免疫血清学和RHD基因型分析。方法:采用常规血型血清学方法鉴定孕妇RhD血型,并进行血型特异性抗体筛查和鉴定;采用序列特异性引物聚合酶链反应(polymerase chain reactionsequence specific primer,PCR-SSP)鉴定孕妇RHD基因型;采用多重连接依赖的探针扩增技术(multiplex ligationdependent probe amplification,MLPA)对孕妇及其配偶和女儿的RhD血型抗原进行基因分型及遗传分析。结果:该孕妇血清中检测出IgG抗-D,其抗体效价为1∶8。PCR-SSP结果显示,该孕妇RHD基因第3-6外显子缺失,经鉴定该孕妇RHD基因型为DVI type 3型。MLPA分析显示,该孕妇只有1条RHD等位基因,且缺失3-6外显子,其基因型为CDVIe/cde,其配偶为CDe/CDe纯合子基因型,女儿为CDe/CDVIe基因型。结论:准确的RhD血型鉴定对制定安全有效的临床输血策略和对育龄妇女采取恰当措施及预防新生儿溶血病具有重要意义。展开更多
2014年10月美国《Otolaryngology-Head and Neck Surgery》杂志增刊发表了《耳鸣临床应用指南(Clinical Practice Guideline:Tinnitus)》。该《指南》由David E.Tunkel等23位学者共同制定,是首部用于评估和治疗慢性耳鸣的指南,旨在为从...2014年10月美国《Otolaryngology-Head and Neck Surgery》杂志增刊发表了《耳鸣临床应用指南(Clinical Practice Guideline:Tinnitus)》。该《指南》由David E.Tunkel等23位学者共同制定,是首部用于评估和治疗慢性耳鸣的指南,旨在为从事耳鸣研究与治疗的专业人员提供基于循证医学证据的建议。《指南》涉及的患者群体是患有持续失代偿性的原发性耳鸣患者(18岁及以上)。本文试结合我国目前耳鸣诊治现状将该《指南》作一解读。展开更多
基金Supported by the Central-Level Non-profit Scientific Research Institutes Special Funds (201104)the Natural Science Foundation of Heilongjiang Province of China (C201140)
文摘A total of 59 isolates of Aeromonas hydrophila were collected from common carp suffering from freshwater fish hemorrhage disease in 13 fishing grounds in the northeast China, and their phenotypic and genetic characteristics were investigated. All of the isolates were identified as A. hydrophila by traditional biochemical method and yielded a 686-bp DNA fragment of the 16S rDNA gene in the PCR experiments. Collected strains were also evaluated for their susceptibility to 17 different antibiotics. The isolates showed an even trend of the resistance and sensitivity to drugs, highly sensitive to antibiotics, such as Levofloxacin, PolymyxinB, Ofloxacin and resistant to antibiotics, such as Bristopen, Lincomycin, Ampicillin, Teicoplanin. Evaluation of genetic diversity was performed on all isolates by molecular typing with enterobacterial repetitive intergenic consensus （ERIC-PCR） method. The results showed that three different types, i.e. type I, ]I, and type ~I, were found in 59 isolates and type III accounted for a large proportion of 54.84%. There was no dominant difference between the tendency of the isolates of Heilongjiang Province and Jilin Province in these three types, which showed DI 〉 I 〉 I, while the isolates of Liaoning Province showed III 〉 II 〉 I. The percentage of different types in different provinces varied in each other; however, they didn＇t show any obvious regional or cluster-specific branches. In conclusion, the ability to distinguish Aeromonas hydrophila strains from diseased common carp with ERIC-PCR would be useful for epidemiological investigation and population genetic analysis of this pathogen in China.
文摘Establishing Consensus with Users of Research Irradiator Devices to Facilitate Source Type Replacement Danette R.Fennesy1,Janet M.Gutiérrez1,2,Scott J.Patlovich1,Robert J.Emery1(1.The University of Texas Health Science Center at Houston,Environmental Health&Safety,6431 Fannin St,CYF G.102,Houston,TX,77030;2.Corresponding author)Abstract:The ability to irradiate cells,tissues,and other biological materials with high-energy photons has been an essential tool in the discovery of numerous biomedical research advancements.
基金Supported by Scientific Research Fund of Hunan Provincial Education Departmen(t23A0361)。
文摘An evolution inequality of Sobolev type involving a nonlinear convolution term is considered.By using the nonlinear capacity method and the contradiction argument,the non-existence of the nontrivial local weak solution is proved.
文摘1)Bifidobacterium longum subsp.iuvenis(Modesto et al.IJSEM.2023,73:6013;Type strain:NCC 5000 T=CCOS 2034 T=LMG 32752^(T))Etymology:iu’ve.nis.L.gen.masc./fem.n.iuvenis,of child,because the type strain was isolated from a 2 year old Bangladeshi child.长双歧杆菌幼儿亚种.
文摘2025年6月16日,上海交通大学医学院附属第一人民医院王宏林团队在Immunity杂志在线发表了题为“Nociceptor-derived CGRP enhances dermal type I conventional dendritic cell function to drive autoreactive CD8+T cell responses in vitiligo”的研究论文。该研究联合单细胞测序、空间转录组测序、皮肤全包埋透明化成像、近10种基因工程小鼠与研究者发起临床试验(investigator-initiated trial,IIT),揭示了白癜风中存在“感觉神经元-CGRP-cDC1-CD8^(+)T细胞”致病新机制;通过CGRP受体拮抗剂(rimegepant)阻断该神经-免疫互作轴,显著抑制了白癜风模型小鼠的疾病进展,并在57例白癜风患者的临床试验中展现出良好的治疗效果。该研究为白癜风治疗策略研发提供了新的理论依据和靶标,并形成了完整的“临床-基础-临床”的研究环路。
基金supported by the National Social Science Fundation(Grant No.21BTJ040)the Project of Outstanding Young People in University of Anhui Province(Grant Nos.2023AH020037,SLXY2024A001).
文摘In this paper,by utilizing the Marcinkiewicz-Zygmund inequality and Rosenthal-type inequality of negatively superadditive dependent(NSD)random arrays and truncated method,we investigate the complete f-moment convergence of NSD random variables.We establish and improve a general result on the complete f-moment convergence for Sung’s type randomly weighted sums of NSD random variables under some general assumptions.As an application,we show the complete consistency for the randomly weighted estimator in a nonparametric regression model based on NSD errors.
基金Projects(52334003,52104111,52274249)supported by the National Natural Science Foundation of ChinaProject(2022YFC2903901)supported by the National Key R&D Project of ChinaProject(2024JJ4064)supported by the Natural Science Foundation of Hunan Province,China。
文摘Water-coupled charge blasting is a promising technique to efficiently break rock masses.In this study,numerical models of double boreholes with water-coupled charge are established using LS-DYNA and are calibrated by the tests of rock masses subjected to explosion loads to examine its performance.The crack levels of rock mass induced by water-coupled charge blasting and air-coupled charge blasting are first compared.It is found that water-coupled charge blasting is more appropriate to fracture deep rock mass than air-coupled charge blasting.In addition,the effects of rock properties,water-coupled charge coefficients,and borehole connection angles on the performance of water-coupled charge blasting are investigated.The results show that rock properties and water-coupled charge coefficients can greatly influence the crack and fragmentation levels of rock mass induced by water-coupled charge blasting under uniform and non-uniform in-situ stresses.However,changing borehole-connection angles can only affect crack and fragmentation levels of rock mass under non-uniform in-situ stresses but barely affect those under uniform in-situ stresses.A formula is finally proposed by considering the above-mentioned factors to provide the design suggestion of water-coupled charge blasting to fracture rock mass with different in-situ stresses.
文摘Different solvothermal reactions of ZnC2O_(4)with oxalic acid(H_(2)ox)and 1,2,4-triazole(Htrz)successfully gave a new quaternary(NJTU-Bai83,NJTU-Bai=Nanjing Tech University Bai's group)and a new quinary(NJTU-Bai84)anionic metal-organic frameworks(MOFs),where NJTU-Bai83=(Me_(2)NH_(2))2[Zn_(3)(trz)_(2)(ox)_(3)]·2H_(2)O and NJTU-Bai84=(Me_(2)NH_(2))[Zn_(3)(trz)_(3)(ox)_(2)]·H_(2)O,respectively.With the[Zn_(2)(ox)4(trz)_(2)]secondary building unit(SBU)in NJTU-Bai83 replaced by the[Zn_(3)(ox)_(2)(trz)_(6)]and planar[Zn(ox)_(2)(trz)_(2)]ones in NJTU-Bai84,2D supramolecular building layers(SBLs)are changed from the A-layer and B-layer to another A-layer,while pillars are transformed from the tetrahedral[Zn(ox)_(2)(trz)_(2)]SBU to the irregular tetrahedral[Zn(ox)_(2)(trz)_(2)]and planar[Zn(ox)_(2)(trz)_(2)]SBUs.Thus,cdq-topological quaternary NJTU-Bai83 is tuned to(4,4,8)-c new topological quinary NJTU-Bai84.Two MOFs were well characterized by powder X-ray diffraction,thermogravimetric analysis,elemental analysis,etc.CCDC:2351819,NJTU-Bai83;2351820,NJTU-Bai84.
文摘目的探讨手机微信管理模式对初发老年2型糖尿病患者健康管理的效果。方法选取2014年1—2月在江西省人民医院内分泌科门诊就诊筛查出的老年初发2型糖尿病患者80例,根据随机数字表法分为试验组和对照组,每组40例。对照组采用门诊常规管理模式,试验组采用手机微信管理模式,均干预6个月。比较两组入组及干预后3、6个月时躯体健康监测指标和糖化血红蛋白(Hb A1c)、血压、低密度脂蛋白胆固醇(LDL-C)达标率及低血糖发生率、糖尿病控制状况。结果两组性别、年龄、文化程度、婚姻状况及高血压、高脂血症发生率间差异均无统计学意义(P>0.05)。两组空腹血糖(FPG)、Hb A1c、BMI间差异无统计学意义(P>0.05),不同时间点FPG、Hb A1c、BMI间差异有统计学意义(P<0.05),干预方法和时间在FPG、Hb A1c、BMI上存在交互作用(P<0.05);两组餐后2h血糖(2 h PG)、收缩压、舒张压、总胆固醇(TC)、三酰甘油(TG)、LDL-C间差异有统计学意义(P<0.05),不同时间点2 h PG、收缩压、舒张压、TC、TG、LDL-C间差异有统计学意义(P<0.05),干预方法和时间在2 h PG、收缩压、舒张压、TC、TG、LDL-C上存在交互作用(P<0.05)。两组干预前低血糖发生率均为0,两组干预前Hb A1c、血压、LDL-C达标率间差异无统计学意义(P>0.05);两组干预后3个月Hb A1c、血压达标率及低血糖发生率间差异无统计学意义(P>0.05),而LDL-C达标率间差异有统计学意义(P<0.05);两组干预后6个月Hb A1c、LDL-C达标率间差异有统计学意义(P<0.05),而血压达标率及低血糖发生率间差异无统计学意义(P>0.05)。两组糖尿病及并发症自觉症状得分间差异无统计学意义(P>0.05),不同时间点糖尿病及并发症自觉症状得分间差异有统计学意义(P<0.05),干预方法和时间在糖尿病及并发症自觉症状得分上存在交互作用(P<0.05);两组生活习惯、治疗情况、生存技能、治疗目标、知识结构得分间差异有统计学意义(P<0.05),不同时间点生活习惯、治疗情况、生存技能、治疗目标、知识结构得分间差异有统计学意义(P<0.05),干预方法和时间在生活习惯、治疗情况、生存技能、治疗目标、知识结构得分上存在交互作用(P<0.05)。结论手机微信管理模式对初发老年2型糖尿病患者健康管理效果较好,值得推广。
文摘A pair of specific primers were designed and synthesized according to the published sequences of ORF1 gene of PCV 2. The complete DNA fragment of ORF1 gene was obtained by PCR from viral DNA of PCV 2 QD strain.Its nuclectide sequence was determined by the dideoxy mediated chain termination method.The results showed that the complete open reading frame (ORF) of ORF1 gene encoding 314 amino acids was 945 bp in length.A comparison of the nucleotide and amino acid sequences of ORF1 gene with that of other PCV strains showed that the identity of nucleotide with PCV 1 and PCV 2 were 83% and 96 4%～99 2% respectively,and identity of the deduced amino acid with PCV 1 and PCV 2 were 84% and more than 98% respecitively.The DNA fragment of ORF1 gene was subcloned into prokaryotic expression vector pET 28a and pGEX KG;while the specific non fusion and fusion proteins with GST of molecular weight 38 kD and 63 kD were expressed in E.coli BL 21 (DE3).Western blotting assay indicated that the polyclonal antibody against PCV 2 could recognize these two proteins.
文摘云南猝死综合征（Yunnan sudden death syndrome，YSDS）是一种每年6～9月份发生在云南省中部和西北部海拔1815～2225 m的山区、半山区，以突然发病并死亡为特征且病因不明的疾病。该病以青壮年为主，女性多于男性，傈僳族、彝族和苗族发病率较高，具有家庭聚集性，临床表现多样，病理学特点缺乏特异性，发病原因与卫生、经济条件、硒铬微量元素缺乏、柯萨奇B组病毒感染、食用蘑菇及当地特殊地质条件相关。本文综述了YSDS的流行病学特征、临床表现、病理性特点、病因研究及假说，以期对YSDS进一步研究提供线索。
文摘目的:对部分D表型孕妇进行免疫血清学和RHD基因型分析。方法:采用常规血型血清学方法鉴定孕妇RhD血型,并进行血型特异性抗体筛查和鉴定;采用序列特异性引物聚合酶链反应(polymerase chain reactionsequence specific primer,PCR-SSP)鉴定孕妇RHD基因型;采用多重连接依赖的探针扩增技术(multiplex ligationdependent probe amplification,MLPA)对孕妇及其配偶和女儿的RhD血型抗原进行基因分型及遗传分析。结果:该孕妇血清中检测出IgG抗-D,其抗体效价为1∶8。PCR-SSP结果显示,该孕妇RHD基因第3-6外显子缺失,经鉴定该孕妇RHD基因型为DVI type 3型。MLPA分析显示,该孕妇只有1条RHD等位基因,且缺失3-6外显子,其基因型为CDVIe/cde,其配偶为CDe/CDe纯合子基因型,女儿为CDe/CDVIe基因型。结论:准确的RhD血型鉴定对制定安全有效的临床输血策略和对育龄妇女采取恰当措施及预防新生儿溶血病具有重要意义。
文摘2014年10月美国《Otolaryngology-Head and Neck Surgery》杂志增刊发表了《耳鸣临床应用指南(Clinical Practice Guideline:Tinnitus)》。该《指南》由David E.Tunkel等23位学者共同制定,是首部用于评估和治疗慢性耳鸣的指南,旨在为从事耳鸣研究与治疗的专业人员提供基于循证医学证据的建议。《指南》涉及的患者群体是患有持续失代偿性的原发性耳鸣患者(18岁及以上)。本文试结合我国目前耳鸣诊治现状将该《指南》作一解读。
