目的验证TIDE(Track of Indels by Decomposition)和TIDER(Tracking of Insertion,DEletions and Recombination events)分析绵羊囊胚基因组编辑结果的可靠性,为检测基因组编辑绵羊胚胎突变效率提供精准、及时的分析方法。方法TIDE是1...目的验证TIDE(Track of Indels by Decomposition)和TIDER(Tracking of Insertion,DEletions and Recombination events)分析绵羊囊胚基因组编辑结果的可靠性,为检测基因组编辑绵羊胚胎突变效率提供精准、及时的分析方法。方法TIDE是1种简便精准的检测方法,能精确测定CRISPR/Cas9在细胞群体中引发的靶向突变谱型和频率。利用TIDE平台对绵羊囊胚的基因组诱导突变进行表征分析与量化。结果以MSTN基因突变绵羊的基因组样本为材料,进行PCR扩增并构建T载体,随机选取单克隆测序。测序结果证实了TIDE分析基因组编辑结果的准确性。通过将TIDER分析单链寡核苷酸模板整合产生的编辑结果与DNA限制性酶切图谱对比,验证了TIDER分析外源DNA短片段导入的准确性。结论TIDE和TIDER工具可有效量化绵羊囊胚突变率,识别主要的插入和缺失类型,是高效经济的基因突变分析方法。展开更多
Nonlinear interactions between the quasi 5-day wave and tides based on meteor radar observation in the Mesosphere and Lower Thermosphere(MLT) at Maui are studied in this paper.Strong sum interaction between quasi 5-da...Nonlinear interactions between the quasi 5-day wave and tides based on meteor radar observation in the Mesosphere and Lower Thermosphere(MLT) at Maui are studied in this paper.Strong sum interaction between quasi 5-day wave and diurnal tide,and evident difference interaction between quasi 5-day wave and semidiurnal tide are observed during the time of attention.However,their difference and sum counterparts are clearly weaker.The secondary waves generated from those interactions beat with the tide and show intense modulation at the period of 5 days which confirms the existence of their interactions.Additionally,correlation coefficients among these waves are calculated to further explore their interactions and find that they can persist for several days although they are highly intermittent.The energy exchange among these waves can be reversible during the observational time.The periods when the significant difference interaction between the quasi 5-day wave and semidiurnal tide occur are much shorter than those when the significant sum interaction between the quasi 5-day wave and diurnal tide occur.Moreover,these two strong interactions can take place simultaneously.In generally,this study provides the proof of nonlinear interactions between quasi 5-day wave and tides which were seldom reported before.展开更多
潟湖体系的潮汐动力对其地貌演变、生态环境保护与海岸带资源的可持续开发利用等具有重要影响。以广东惠州双月湾潟湖为研究靶区,基于2020—2023年4次潮位观测资料,采用基于光滑导纳原理的短时调和分析(modified harmonic analysis mode...潟湖体系的潮汐动力对其地貌演变、生态环境保护与海岸带资源的可持续开发利用等具有重要影响。以广东惠州双月湾潟湖为研究靶区,基于2020—2023年4次潮位观测资料,采用基于光滑导纳原理的短时调和分析(modified harmonic analysis model on the basis of the credo of smoothness,MHACS)模型和数理统计方法初步探讨该潟湖体系潮波传播和变化过程。结果表明,双月湾潟湖体系平均潮差为0.52~0.69m,潮波从口门传播至潟湖上段,其能量明显减小,特别是上游区域的潮波衰减率为近口门段20倍,潮波传播速度亦降低至近口门段的12%。在潟湖中,半日潮M2、全日潮K1和浅水潮M4占据主导,其振幅分别为23.06cm、30.4cm和7.74cm。其中,全日潮和半日潮的振幅月变化较为稳定,而浅水分潮的季节波动更加明显,尤其是向上游传播时,分潮的振幅和迟角季节性变化更为显著。潮波通过口门传入双月湾潟湖,受地形辐聚与底床摩擦效应影响,不同河段的潮波传播速度差异显著,尤其是半日潮在口门区的传播速度普遍高于全日潮。各分潮在潟湖内的振幅衰减明显,特别是S2、M4和MS4分潮的振幅梯度最为显著(4×10^(−6)m^(−1))。展开更多
文摘目的验证TIDE(Track of Indels by Decomposition)和TIDER(Tracking of Insertion,DEletions and Recombination events)分析绵羊囊胚基因组编辑结果的可靠性,为检测基因组编辑绵羊胚胎突变效率提供精准、及时的分析方法。方法TIDE是1种简便精准的检测方法,能精确测定CRISPR/Cas9在细胞群体中引发的靶向突变谱型和频率。利用TIDE平台对绵羊囊胚的基因组诱导突变进行表征分析与量化。结果以MSTN基因突变绵羊的基因组样本为材料,进行PCR扩增并构建T载体,随机选取单克隆测序。测序结果证实了TIDE分析基因组编辑结果的准确性。通过将TIDER分析单链寡核苷酸模板整合产生的编辑结果与DNA限制性酶切图谱对比,验证了TIDER分析外源DNA短片段导入的准确性。结论TIDE和TIDER工具可有效量化绵羊囊胚突变率,识别主要的插入和缺失类型,是高效经济的基因突变分析方法。
基金Supported by the National Natural Science Foundation of China(41174126)the Specialized Fund for Comprehensive Study and Evaluation of the Polar Environment(CHINARE2014-02-03)
文摘Nonlinear interactions between the quasi 5-day wave and tides based on meteor radar observation in the Mesosphere and Lower Thermosphere(MLT) at Maui are studied in this paper.Strong sum interaction between quasi 5-day wave and diurnal tide,and evident difference interaction between quasi 5-day wave and semidiurnal tide are observed during the time of attention.However,their difference and sum counterparts are clearly weaker.The secondary waves generated from those interactions beat with the tide and show intense modulation at the period of 5 days which confirms the existence of their interactions.Additionally,correlation coefficients among these waves are calculated to further explore their interactions and find that they can persist for several days although they are highly intermittent.The energy exchange among these waves can be reversible during the observational time.The periods when the significant difference interaction between the quasi 5-day wave and semidiurnal tide occur are much shorter than those when the significant sum interaction between the quasi 5-day wave and diurnal tide occur.Moreover,these two strong interactions can take place simultaneously.In generally,this study provides the proof of nonlinear interactions between quasi 5-day wave and tides which were seldom reported before.
文摘潟湖体系的潮汐动力对其地貌演变、生态环境保护与海岸带资源的可持续开发利用等具有重要影响。以广东惠州双月湾潟湖为研究靶区,基于2020—2023年4次潮位观测资料,采用基于光滑导纳原理的短时调和分析(modified harmonic analysis model on the basis of the credo of smoothness,MHACS)模型和数理统计方法初步探讨该潟湖体系潮波传播和变化过程。结果表明,双月湾潟湖体系平均潮差为0.52~0.69m,潮波从口门传播至潟湖上段,其能量明显减小,特别是上游区域的潮波衰减率为近口门段20倍,潮波传播速度亦降低至近口门段的12%。在潟湖中,半日潮M2、全日潮K1和浅水潮M4占据主导,其振幅分别为23.06cm、30.4cm和7.74cm。其中,全日潮和半日潮的振幅月变化较为稳定,而浅水分潮的季节波动更加明显,尤其是向上游传播时,分潮的振幅和迟角季节性变化更为显著。潮波通过口门传入双月湾潟湖,受地形辐聚与底床摩擦效应影响,不同河段的潮波传播速度差异显著,尤其是半日潮在口门区的传播速度普遍高于全日潮。各分潮在潟湖内的振幅衰减明显,特别是S2、M4和MS4分潮的振幅梯度最为显著(4×10^(−6)m^(−1))。
