【Objective】This study aimed to clarify the key pathways and related genes of taro leaves in response to drought stress,analyze the gene expression patterns under drought conditions,and explore the molecular response...【Objective】This study aimed to clarify the key pathways and related genes of taro leaves in response to drought stress,analyze the gene expression patterns under drought conditions,and explore the molecular response mecha‐nisms.The findings would provide theoretical references for understanding the molecular mechanisms of taro’s drought regulation and for breeding different drought tolerant taro varieties in the future.【Method】Using Lipu taro as the experi‐mental material,leaf samples were collected after consecutive 7 d of drought treatment as the treatment group,while leaf samples from plants watered daily served as the control group.Transcriptome sequencing was performed to identify dif‐ferentially expressed genes,which were then subjected to GO functional annotation and KEGG pathway enrichment analysis.【Result】Under drought stress,there were 1613 differentially expressed genes(DEGs),including 1043 upregulated and 570 down-regulated genes.GO functional annotation analysis revealed that the DEGs were categorized into three major functional groups:molecular function,cellular component,and biological process.In the molecular function category,DEGs were annotated to binding and catalytic activity.In the cellular component category,DEGs were anno‐tated to cellular anatomical entities and protein-containing complexes.In the biological process category,DEGs were an‐notated to cellular processes and metabolic processes.KEGG signaling pathway enrichment analysis showed that 85.00%of the DEGs were enriched in metabolic pathway.Among these,the DEGs were primarily enriched in the glutathione me‐tabolism pathway and the starch and sucrose metabolism pathway,with 11 and 19 DEGs identified in each pathway re‐spectively.Under drought stress,a total of 112 differentially expressed transcription factors(TFs)were identified,mainly including members of the bHLH,ERF,WRKY and NAC families.Among all differentially expressed TFs,82.14%showed up-regulated transcription levels under drought conditions.Plant hormone signal transduction,carotenoid biosynthesis,and the MAPK signaling pathway-plant were identified as key abscisic acid-responsive pathways involved in drought response,influencing stomatal closure in taro leaves and seed dormancy to cope with drought stress.The reliability of the transcriptome data was confirmed by quantitative real-time PCR analysis.【Conclusion】Under drought stress,the gene expression in the glutathione metabolism pathway,the starch and sucrose metabolism pathway,and transcription factors in taro leaves is affected.Most TFs are positively involved in regulating taro plant’s drought response.展开更多
本研究以荔浦芋头为材料,经人工损伤处理后,以自然愈伤(CK)为对照,65℃热水快速热激处理(heat water treatment,HWT)和30℃热空气处理(hot air treatment,HAT)为实验组,分别在0、2、4、6、14 d观察愈伤期间芋头球茎伤口外观、木栓层截...本研究以荔浦芋头为材料,经人工损伤处理后,以自然愈伤(CK)为对照,65℃热水快速热激处理(heat water treatment,HWT)和30℃热空气处理(hot air treatment,HAT)为实验组,分别在0、2、4、6、14 d观察愈伤期间芋头球茎伤口外观、木栓层截面厚度、木质素染色情况;同时测定芋头球茎愈伤组织形成期间伤口处活性氧含量和代谢酶、木质素积累和合成酶活性变化规律。结果表明,在愈伤形成过程中,愈伤组织木栓层厚度、总酚、类黄酮、木质素积累不断增加,HAT组含量显著高于其他两组,且HAT组在2~4 d时,即可形成致密的愈伤木栓层,明显早于其他两处理组。在2 d时,HAT组较CK和HWT组O_(2)^(-)·和H_(2)O_(2)迅速积累,活性氧清除酶超氧化物歧化酶、过氧化氢酶活性出现峰值(分别为1685.628、37.380 U/g,P<0.05),木质素合成酶苯丙氨酸解氨酶和4-香豆酸:辅酶A连接酶活性均在此刻达到酶活性峰值(分别为544.697、123.221 U/g,P<0.05),过氧化物酶活性不断增加,显著高于CK和HWT组。综上所述,HAT可诱导活性氧积累和代谢的快速响应,加速苯丙烷代谢途径中关键酶的生成,促进总酚、类黄酮、木质素积累,加速芋头球茎愈伤组织的形成。展开更多
基金National Natural Science Foundation of China(32460756)Guangxi Key Research and Development Project(Guike AB20297041)+1 种基金Science and Technology Development Fund of Guangxi Academy of Agricultural Sciences(Gui‐nongke 2022JM58)Guangxi Lipu Taro Experimental Station Projec(tTS202113)。
文摘【Objective】This study aimed to clarify the key pathways and related genes of taro leaves in response to drought stress,analyze the gene expression patterns under drought conditions,and explore the molecular response mecha‐nisms.The findings would provide theoretical references for understanding the molecular mechanisms of taro’s drought regulation and for breeding different drought tolerant taro varieties in the future.【Method】Using Lipu taro as the experi‐mental material,leaf samples were collected after consecutive 7 d of drought treatment as the treatment group,while leaf samples from plants watered daily served as the control group.Transcriptome sequencing was performed to identify dif‐ferentially expressed genes,which were then subjected to GO functional annotation and KEGG pathway enrichment analysis.【Result】Under drought stress,there were 1613 differentially expressed genes(DEGs),including 1043 upregulated and 570 down-regulated genes.GO functional annotation analysis revealed that the DEGs were categorized into three major functional groups:molecular function,cellular component,and biological process.In the molecular function category,DEGs were annotated to binding and catalytic activity.In the cellular component category,DEGs were anno‐tated to cellular anatomical entities and protein-containing complexes.In the biological process category,DEGs were an‐notated to cellular processes and metabolic processes.KEGG signaling pathway enrichment analysis showed that 85.00%of the DEGs were enriched in metabolic pathway.Among these,the DEGs were primarily enriched in the glutathione me‐tabolism pathway and the starch and sucrose metabolism pathway,with 11 and 19 DEGs identified in each pathway re‐spectively.Under drought stress,a total of 112 differentially expressed transcription factors(TFs)were identified,mainly including members of the bHLH,ERF,WRKY and NAC families.Among all differentially expressed TFs,82.14%showed up-regulated transcription levels under drought conditions.Plant hormone signal transduction,carotenoid biosynthesis,and the MAPK signaling pathway-plant were identified as key abscisic acid-responsive pathways involved in drought response,influencing stomatal closure in taro leaves and seed dormancy to cope with drought stress.The reliability of the transcriptome data was confirmed by quantitative real-time PCR analysis.【Conclusion】Under drought stress,the gene expression in the glutathione metabolism pathway,the starch and sucrose metabolism pathway,and transcription factors in taro leaves is affected.Most TFs are positively involved in regulating taro plant’s drought response.
文摘本研究以荔浦芋头为材料,经人工损伤处理后,以自然愈伤(CK)为对照,65℃热水快速热激处理(heat water treatment,HWT)和30℃热空气处理(hot air treatment,HAT)为实验组,分别在0、2、4、6、14 d观察愈伤期间芋头球茎伤口外观、木栓层截面厚度、木质素染色情况;同时测定芋头球茎愈伤组织形成期间伤口处活性氧含量和代谢酶、木质素积累和合成酶活性变化规律。结果表明,在愈伤形成过程中,愈伤组织木栓层厚度、总酚、类黄酮、木质素积累不断增加,HAT组含量显著高于其他两组,且HAT组在2~4 d时,即可形成致密的愈伤木栓层,明显早于其他两处理组。在2 d时,HAT组较CK和HWT组O_(2)^(-)·和H_(2)O_(2)迅速积累,活性氧清除酶超氧化物歧化酶、过氧化氢酶活性出现峰值(分别为1685.628、37.380 U/g,P<0.05),木质素合成酶苯丙氨酸解氨酶和4-香豆酸:辅酶A连接酶活性均在此刻达到酶活性峰值(分别为544.697、123.221 U/g,P<0.05),过氧化物酶活性不断增加,显著高于CK和HWT组。综上所述,HAT可诱导活性氧积累和代谢的快速响应,加速苯丙烷代谢途径中关键酶的生成,促进总酚、类黄酮、木质素积累,加速芋头球茎愈伤组织的形成。
