Anthocyanin,as a typical food bioactive molecule,is capable of reversing inflammatory,oxidative and allergic condition thus contributes to intestinal health.We were wondering whether anthocyanin has influence on the i...Anthocyanin,as a typical food bioactive molecule,is capable of reversing inflammatory,oxidative and allergic condition thus contributes to intestinal health.We were wondering whether anthocyanin has influence on the infiltration of inflammatory cells into the intestinal mucosa and thus help enhancing intestinal barrier which could be damaged in some metabolic diseases.In this study,the influence of anthocyanin(administered orally)on the alterations(including structure and permeability)of the intestinal mucosa in mice in response to a high fat-high cholesterol(HFHC)diet was investigated.Primary T helper 17(Th17)cells were isolated from mouse intestine tissues to observe the modulatory role of anthocyanin through the transcription phosphorylated STAT 3(p-STAT3).The results indicated that anthocyanin significantly alleviated HFHC-induced impairment in the intestinal structures and permeability in a dose-dependent manner;moreover,anthocyanin appeared to inhibit HFHC induced the expression of p-STAT3,thereby disturbing Th17 cell differentiation.In high-fat diet(HFD,cholesterol level non-modified)-challenged mice selective p-STAT3 inhibitor significantly reversed the effects of anthocyanin,which were decreased amount of interleukin(IL)-17A(produced and released from Th17 cells)and the protected intestinal structure/function.In summary,the results of this study suggest that anthocyanin may attenuate the damage of intestinal barrier in HFHC mice through regulating intestinal STAT3-Th17-IL-17A signal transduction pathway.展开更多
It has been revealed that the different morphologies of anodized TiO_2 nanotubes, especially nanotube diameters, triggered different cell behaviors. However, the influence of TiO_2 nanotubes with coexisting multi-size...It has been revealed that the different morphologies of anodized TiO_2 nanotubes, especially nanotube diameters, triggered different cell behaviors. However, the influence of TiO_2 nanotubes with coexisting multi-size diameters on cell behaviors is seldom reported. In this work, coexisting four-diameter TiO_2 nanotube samples, namely,one single substrate with the integration of four different nanotube diameters(60, 150, 250, and 350 nm), were prepared by repeated anodization. The boundaries between two different diameter regions show well-organized structure without obvious difference in height. The adhesion behaviors of MC3T3-E1 cells on the coexisting fourdiameter TiO_2 nanotube arrays were investigated. The results exhibit a significant difference of cell density between smaller diameters(60 and 150 nm) and larger diameters(250 and 350 nm) within 24 h incubation with the coexistence of different diameters, which is totally different from that on the single-diameter TiO_2 nanotube arrays. The coexistence of four different diameters does not change greatly the cell morphologies compared with the singlediameter nanotubes. The findings in this work are expected to offer further understanding of the interaction between cells and materials.展开更多
目的通过检测IL-5与INF-γ在患儿的腺样体组织、中耳积液及血清中的表达,探讨其在儿童分泌性中耳炎(otitis media with effusion,OME)发病机制中的作用。方法将2017年我科住院的符合条件的腺样体肥大患儿,随机分为单纯腺样体肥大组及腺...目的通过检测IL-5与INF-γ在患儿的腺样体组织、中耳积液及血清中的表达,探讨其在儿童分泌性中耳炎(otitis media with effusion,OME)发病机制中的作用。方法将2017年我科住院的符合条件的腺样体肥大患儿,随机分为单纯腺样体肥大组及腺样体肥大伴OME组患儿各20例,检测OME患儿腺样体组织、中耳积液及血清中IL-5与INF-γ的表达,并进行比较。结果腺样体肥大组与OME组两组间比较腺样体组织中IL-5表达,差异有统计学意义(t=-3.211,P<0.05)。两组间比较血清中IL-5表达,差异无统计学意义(P>0.05)。伴发OME组内比较IL-5的表达:腺样体组织与血清比较,差异有统计学意义(t=-8.955,P<0.05)。腺样体组织与中耳积液比较,差异有统计学意义(t=-7.486,P<0.05)。血清与中耳积液比较,差异无统计学意义(P>0.05)。腺样体肥大组与OME组两组间比较腺样体组织中INF-γ表达,差异无统计学意义(t=1.038,P>0.05)。两组间比较血清中INF-γ表达,差异无统计学意义(t=-0.509,P>0.05)。伴发OME组内比较INF-γ的表达:腺样体组织与血清比较,差异有统计学意义(t=-9.810,P<0.05)。腺样体组织与中耳积液比较,差异有统计学意义(t=-8.075,P<0.05)。血清与中耳积液比较,差异无统计学意义(t=-0.062,P>0.05)。结论 Th2型细胞因子活化亢进,Th2/Th1失衡,导致腺样体局部免疫异常,可能是儿童腺样体肥大伴分泌性中耳炎的重要发病基础。展开更多
基金supported by the National Natural Science Foundation of China(81973022 and 81730090)。
文摘Anthocyanin,as a typical food bioactive molecule,is capable of reversing inflammatory,oxidative and allergic condition thus contributes to intestinal health.We were wondering whether anthocyanin has influence on the infiltration of inflammatory cells into the intestinal mucosa and thus help enhancing intestinal barrier which could be damaged in some metabolic diseases.In this study,the influence of anthocyanin(administered orally)on the alterations(including structure and permeability)of the intestinal mucosa in mice in response to a high fat-high cholesterol(HFHC)diet was investigated.Primary T helper 17(Th17)cells were isolated from mouse intestine tissues to observe the modulatory role of anthocyanin through the transcription phosphorylated STAT 3(p-STAT3).The results indicated that anthocyanin significantly alleviated HFHC-induced impairment in the intestinal structures and permeability in a dose-dependent manner;moreover,anthocyanin appeared to inhibit HFHC induced the expression of p-STAT3,thereby disturbing Th17 cell differentiation.In high-fat diet(HFD,cholesterol level non-modified)-challenged mice selective p-STAT3 inhibitor significantly reversed the effects of anthocyanin,which were decreased amount of interleukin(IL)-17A(produced and released from Th17 cells)and the protected intestinal structure/function.In summary,the results of this study suggest that anthocyanin may attenuate the damage of intestinal barrier in HFHC mice through regulating intestinal STAT3-Th17-IL-17A signal transduction pathway.
基金supported by the National Natural Science Foundation of China(No.51401126,No.51271117)Shanghai Committee of Science and Technology,China(No.14441901800)
文摘It has been revealed that the different morphologies of anodized TiO_2 nanotubes, especially nanotube diameters, triggered different cell behaviors. However, the influence of TiO_2 nanotubes with coexisting multi-size diameters on cell behaviors is seldom reported. In this work, coexisting four-diameter TiO_2 nanotube samples, namely,one single substrate with the integration of four different nanotube diameters(60, 150, 250, and 350 nm), were prepared by repeated anodization. The boundaries between two different diameter regions show well-organized structure without obvious difference in height. The adhesion behaviors of MC3T3-E1 cells on the coexisting fourdiameter TiO_2 nanotube arrays were investigated. The results exhibit a significant difference of cell density between smaller diameters(60 and 150 nm) and larger diameters(250 and 350 nm) within 24 h incubation with the coexistence of different diameters, which is totally different from that on the single-diameter TiO_2 nanotube arrays. The coexistence of four different diameters does not change greatly the cell morphologies compared with the singlediameter nanotubes. The findings in this work are expected to offer further understanding of the interaction between cells and materials.
文摘目的通过检测IL-5与INF-γ在患儿的腺样体组织、中耳积液及血清中的表达,探讨其在儿童分泌性中耳炎(otitis media with effusion,OME)发病机制中的作用。方法将2017年我科住院的符合条件的腺样体肥大患儿,随机分为单纯腺样体肥大组及腺样体肥大伴OME组患儿各20例,检测OME患儿腺样体组织、中耳积液及血清中IL-5与INF-γ的表达,并进行比较。结果腺样体肥大组与OME组两组间比较腺样体组织中IL-5表达,差异有统计学意义(t=-3.211,P<0.05)。两组间比较血清中IL-5表达,差异无统计学意义(P>0.05)。伴发OME组内比较IL-5的表达:腺样体组织与血清比较,差异有统计学意义(t=-8.955,P<0.05)。腺样体组织与中耳积液比较,差异有统计学意义(t=-7.486,P<0.05)。血清与中耳积液比较,差异无统计学意义(P>0.05)。腺样体肥大组与OME组两组间比较腺样体组织中INF-γ表达,差异无统计学意义(t=1.038,P>0.05)。两组间比较血清中INF-γ表达,差异无统计学意义(t=-0.509,P>0.05)。伴发OME组内比较INF-γ的表达:腺样体组织与血清比较,差异有统计学意义(t=-9.810,P<0.05)。腺样体组织与中耳积液比较,差异有统计学意义(t=-8.075,P<0.05)。血清与中耳积液比较,差异无统计学意义(t=-0.062,P>0.05)。结论 Th2型细胞因子活化亢进,Th2/Th1失衡,导致腺样体局部免疫异常,可能是儿童腺样体肥大伴分泌性中耳炎的重要发病基础。
