Polysaccharide production from tea flower(TFPS) was carried out using supercritical fluid extraction(SFE).Response surface methodology(RSM),based on a five level,four variable small central composite design,was employ...Polysaccharide production from tea flower(TFPS) was carried out using supercritical fluid extraction(SFE).Response surface methodology(RSM),based on a five level,four variable small central composite design,was employed to obtain the best possible combination of extraction time,pressure,temperature and ethanol content of modifier for maximum production.The optimum conditions were as follows:extraction time of 170 min,pressure of45 MPa,temperature of 75 ℃,and 50% aqueous ethanol solution as modifier.Under these conditions,the experimental yield was 6.56 ± 0.37%,which was similar to the value predicted by the model.Monosaccharide composition of TFPS was fucose,rhamnose,arabinose,xylose,galactose,glucose,mannose,fructose,ribose,galacturonic acid and glucuronic acid in a molar percent of 31.69,0.21,0.49,1.29,35.82,0.97,1.63,18.34,7.88,1.06 and 0.63.Compared to other extraction methods,SFE could achieve higher yield and gain more types of monosaccharide.展开更多
Objective:Aim To analyze the chemical constituents of essential oil from the fruits of Bupleurum chinense DC.Methods:Essential oil from the fruits of Bupleurum chinense DC.was extracted by using supercritical fluid ex...Objective:Aim To analyze the chemical constituents of essential oil from the fruits of Bupleurum chinense DC.Methods:Essential oil from the fruits of Bupleurum chinense DC.was extracted by using supercritical fluid extraction (SFE),and the chemical constituents to be identified by GC/MS spectrum.Results:The yield of essential oil from the fruits of Bupleurum chinense DC.by SFE was 6.60%(V/W)and 73 components were identified in this oil.Conclusions:The essential oil from the fruits of Bupleurum chinense DC.was produced for the first time,and its yield was 3 times more than its root,the relative contents of linoleic acid,cetyl acetate,ethyl linoleate,(-)-caryophyllene oxide are 34.85%,16.63%,2.64%,1.81% apart in this essential oil.Phytol,cetyl acetate,ethyl linoleate,(-)-caryophyllene oxide were confirmed for the first time from the genus of Bupleurum.展开更多
A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC)system was established.The interfere consisted of automated ten-port valve and parallel traps were used to ...A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC)system was established.The interfere consisted of automated ten-port valve and parallel traps were used to carry out continuous and alternate trapping and transferring of the sample extracted with supercritical carbon dioxide,and HPLC was used to analyze the transferred sample.The novel on-line system was used to monitor the extraction process of fruiting bodies of Ganoderma lucidum.The extracts of six consecutive cycles were analyzed and the optimum extraction time was determined,which accorded with the result obtained with off-line extraction.Using this system,the whole process can be monitored and the optimum extraction time can be determined with single run.In addition,it has many other advantages,such as low sample loading,high sensitivity and accuracy,short analysis time,avoiding sample pollution and easily operation etc.The system shows a great potential in the extraction and analysis of the complicated samples including traditional Chinese medicine and environmental samples etc.展开更多
基金supported by a key research grant "The application and Utilization of Tea (Camellia sinensis) Flowers" from the 2013 Zhejiang Province
文摘Polysaccharide production from tea flower(TFPS) was carried out using supercritical fluid extraction(SFE).Response surface methodology(RSM),based on a five level,four variable small central composite design,was employed to obtain the best possible combination of extraction time,pressure,temperature and ethanol content of modifier for maximum production.The optimum conditions were as follows:extraction time of 170 min,pressure of45 MPa,temperature of 75 ℃,and 50% aqueous ethanol solution as modifier.Under these conditions,the experimental yield was 6.56 ± 0.37%,which was similar to the value predicted by the model.Monosaccharide composition of TFPS was fucose,rhamnose,arabinose,xylose,galactose,glucose,mannose,fructose,ribose,galacturonic acid and glucuronic acid in a molar percent of 31.69,0.21,0.49,1.29,35.82,0.97,1.63,18.34,7.88,1.06 and 0.63.Compared to other extraction methods,SFE could achieve higher yield and gain more types of monosaccharide.
文摘Objective:Aim To analyze the chemical constituents of essential oil from the fruits of Bupleurum chinense DC.Methods:Essential oil from the fruits of Bupleurum chinense DC.was extracted by using supercritical fluid extraction (SFE),and the chemical constituents to be identified by GC/MS spectrum.Results:The yield of essential oil from the fruits of Bupleurum chinense DC.by SFE was 6.60%(V/W)and 73 components were identified in this oil.Conclusions:The essential oil from the fruits of Bupleurum chinense DC.was produced for the first time,and its yield was 3 times more than its root,the relative contents of linoleic acid,cetyl acetate,ethyl linoleate,(-)-caryophyllene oxide are 34.85%,16.63%,2.64%,1.81% apart in this essential oil.Phytol,cetyl acetate,ethyl linoleate,(-)-caryophyllene oxide were confirmed for the first time from the genus of Bupleurum.
文摘A novel on-line parallel-trap supercritical fluid extraction(SFE)-high performance liquid chromatography(HPLC)system was established.The interfere consisted of automated ten-port valve and parallel traps were used to carry out continuous and alternate trapping and transferring of the sample extracted with supercritical carbon dioxide,and HPLC was used to analyze the transferred sample.The novel on-line system was used to monitor the extraction process of fruiting bodies of Ganoderma lucidum.The extracts of six consecutive cycles were analyzed and the optimum extraction time was determined,which accorded with the result obtained with off-line extraction.Using this system,the whole process can be monitored and the optimum extraction time can be determined with single run.In addition,it has many other advantages,such as low sample loading,high sensitivity and accuracy,short analysis time,avoiding sample pollution and easily operation etc.The system shows a great potential in the extraction and analysis of the complicated samples including traditional Chinese medicine and environmental samples etc.
