Suaeda salsa an annual herb of Chenopodiaceae Suaeda well accepted as both a folk medicinal and food plant.In the current study,the extraction of total flavonoids from S.salsa and the in vitro antioxidant,hypoglycemic...Suaeda salsa an annual herb of Chenopodiaceae Suaeda well accepted as both a folk medicinal and food plant.In the current study,the extraction of total flavonoids from S.salsa and the in vitro antioxidant,hypoglycemic and hypolipidemic activities of the extracts were investigated.The total flavonoids was extracted by refluxing with ethanol and the selection and optimization of main experimental parameters were carried out using single factor experiments combined with central composite design-response surface methodology(CCD-RSM).The total flavonoids extracted from S.salsa were tested for hydroxyl and oxygen radical scavenging activity,and inhibitory activities againstα-amylase and lipase in vitro.Results revealed that the optimal conditions for the extraction of total flavonoids were as following:liquid-tosolid ratio,31.40:1;ethanol concentration,83.12%;reflux temperature,90℃,and reflux time,128.40 min.Under these optimized conditions,the predicted and actual values of total flavonoids yield were 0.896%and 0.932%,respectively.The in vitro antioxidant tests revealed that the total flavonoids could scavenge hydroxyl and oxygen radicals and showed inhibitory activity againstα-amylase and lipase.The optimal extraction conditions obtained in this experiment are simple to perform and have good reproducibility.The results have demonstrated that the total flavonoids from S.salsa can be used as a potential source of natural antioxidant,hypoglycemic and hypolipidemic ingredients,which provides new pharmacological basis for the development of S.salsa.展开更多
文摘Suaeda salsa an annual herb of Chenopodiaceae Suaeda well accepted as both a folk medicinal and food plant.In the current study,the extraction of total flavonoids from S.salsa and the in vitro antioxidant,hypoglycemic and hypolipidemic activities of the extracts were investigated.The total flavonoids was extracted by refluxing with ethanol and the selection and optimization of main experimental parameters were carried out using single factor experiments combined with central composite design-response surface methodology(CCD-RSM).The total flavonoids extracted from S.salsa were tested for hydroxyl and oxygen radical scavenging activity,and inhibitory activities againstα-amylase and lipase in vitro.Results revealed that the optimal conditions for the extraction of total flavonoids were as following:liquid-tosolid ratio,31.40:1;ethanol concentration,83.12%;reflux temperature,90℃,and reflux time,128.40 min.Under these optimized conditions,the predicted and actual values of total flavonoids yield were 0.896%and 0.932%,respectively.The in vitro antioxidant tests revealed that the total flavonoids could scavenge hydroxyl and oxygen radicals and showed inhibitory activity againstα-amylase and lipase.The optimal extraction conditions obtained in this experiment are simple to perform and have good reproducibility.The results have demonstrated that the total flavonoids from S.salsa can be used as a potential source of natural antioxidant,hypoglycemic and hypolipidemic ingredients,which provides new pharmacological basis for the development of S.salsa.
