The solution chemical and optical characteristics of formation of amine-terminated polyamidoamine dendrimer G2.0（NH2-PAMAM G2.0）-Au nanocomposites in the aqueous solution of NH2-PAMAM G2.0 at various mole ratios of...The solution chemical and optical characteristics of formation of amine-terminated polyamidoamine dendrimer G2.0（NH2-PAMAM G2.0）-Au nanocomposites in the aqueous solution of NH2-PAMAM G2.0 at various mole ratios of Au（Ⅲ） to NH2-PAMAM G2.0 were studied by both UV-visible spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle is surrounded by several NH2-PAMAM G2.0 dendrimers, emit strong bluish violet fluorescence, and are uniform, water soluble and biocompatible as well as very stable in frozen conditions. The size of gold nanoparticles in the nanocomposites is about 2.5 nm and decreases with the increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2.0 plays an important role in acting as host or micro-reactor for Au（Ⅲ） before Au（Ⅲ） reduction and acting as dispersant and stabilizer for gold nanoparticles after Au（Ⅲ） reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast cell lines show that the NH2-PAMAM G2.0-Au nanocomposites can be used as optical imaging markers for bioanalyses and medical diagnoses.展开更多
Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ ...Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature展开更多
The functional activity and morphological integrity of the porcine thyroid tissue could be maintained up to 96 hrs with organ culture technique. Tg,T<sub>4</sub> and T<sub>3</sub> showed a almo...The functional activity and morphological integrity of the porcine thyroid tissue could be maintained up to 96 hrs with organ culture technique. Tg,T<sub>4</sub> and T<sub>3</sub> showed a almost similar localization and immunohistochemical staining pattern: diffuse staining of follicular lumen （L）, granular staining of follicular border（G）, and foamy staining of follicular border （F）. The significant differences between the thyrotropinstimulated and non-stimulated groups were observed. The peripheral vacuoles of colloid were increased （P【0.05） in the former. Dominant staining patterns were G and F in the former. This simple organ culture system is a useful model for studying the functional aspects such as hormone biosynthesis in thyroid gland.展开更多
Objective: The objective of research is to explorethe prospective for direct gene transfer to be applied as aform of gene therapy. Materials and methods: ①Experimental animals: Male BALB/c mice, 0 - 8 weeksold;②Prep...Objective: The objective of research is to explorethe prospective for direct gene transfer to be applied as aform of gene therapy. Materials and methods: ①Experimental animals: Male BALB/c mice, 0 - 8 weeksold;②Preparations of plasmid: PCI-hIL-2 plasmid isexpression vector for human IL-2 containing cytome-galovirus immediate-early enhance/promoter region andT7 promoer;③Injection of plasmids: Male展开更多
To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon...To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon 2 was ligated into retroviral vector pDOR-neoin reverse direction. This recombinant plasmid展开更多
To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred ...To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred to mouse mammary carcinoma cell line 4Tlwith the method of infection. The HSV-tk gene in thegenome of transduced cells (4Tl/tk) was demonstratedby the method of DNA-PCR. Using a modified展开更多
In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Amo...In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.展开更多
AIM:To observe the protective effects of 13-methyltetradecanoic acid(13-MTD)on the injury of cultured brain microvascular endothelial cells(BMECs)induced by oxygen paradox. METHODS:Two screen filtration methods and en...AIM:To observe the protective effects of 13-methyltetradecanoic acid(13-MTD)on the injury of cultured brain microvascular endothelial cells(BMECs)induced by oxygen paradox. METHODS:Two screen filtration methods and enzyme digestion method were used to the primary culture and subculture of the neonatal rat cortex BMECs.展开更多
In order to investigate the mechanism of action of immunoenhancer, the effects of the traditional Chinese medicine immunopromoter on the quantity and the transformation rates of T lymphocytes in the chicken blood were...In order to investigate the mechanism of action of immunoenhancer, the effects of the traditional Chinese medicine immunopromoter on the quantity and the transformation rates of T lymphocytes in the chicken blood were determined. Total 120 chickens were randomly assigned into three groups. The 1% and the 0.5% of the Chinese medicine immunopromoter were added to the chicken drinking water, respectively. The quantity of T lymphocytes in each group was measured by a-Naphthyl acetate esterase （ANAE） staining. The results showed that the percentages of T lymphocytes of the treatment groups were significantly higher than that of the control group （P〈0.05）, and the percentage of the 1% group significantly higher than that of the 0.5% group （P〈0.05）. In conclusion, the transformation rates of T lymphocytes showed that the Chinese medicine immunopromoter had the significant enhancing effect on the transformation rates of T lymphocytes of the treated chickens. The traditional Chinese medicine immunopromoter had the distinct function to promote the quantity and the transformation rate of T lymphocytes.展开更多
Based on the characteristics and reinforcement mechanism of vacuum preloading applied in high way soft foundation, the theoretical calculation method of vacuum preloading was presented. According to the equivalent met...Based on the characteristics and reinforcement mechanism of vacuum preloading applied in high way soft foundation, the theoretical calculation method of vacuum preloading was presented. According to the equivalent method derived from sand drain foundation subjected to plane and axisymmetric conditions, the three dimensional problem was transformed into plane strain problem. The modified Komala-Huang model was used to model the rheological behavior and the damage theory was also introduced to consider the effect of damage caused by construction disturbance. Numerical simulations were carried out for a high way test section. The results show that simulations of the viscoelastic-plastic-damage model are in good agreement with the field measurements. It suggests that the calculation method, simplified load and boundary condition used in this study are reasonable.展开更多
This paper discussed 378 cases of lung cancer which were identified pathologically in the thoracic department of China Medical University from 1980～1986. The sections were stained with H.E., PAS-ab and Keratin, and c...This paper discussed 378 cases of lung cancer which were identified pathologically in the thoracic department of China Medical University from 1980～1986. The sections were stained with H.E., PAS-ab and Keratin, and compared with sections of 131 cases of lung cancer being confirmed beforehand from 1978～1984. Chinese cases showed epidermoid carcinoma 165 (male 146), adenocarcinoma 136(male 82), small cell carcinoma 29 (male 21), large cell carcinoma 19 (male 15) and the others 29 (male 27); Japanese cases were 59(50), 53(25), 3 (2), 11(10), 3(0) respectively. The cases under 40 years of age were 23(6.1%) in China, 3(2.3%)in Japan. Both of them have similar character of histology. But the incidence of lung cancer in youth is higher in China.展开更多
Liposomes have several advantages over viral vectorsfor gene delivery both in vitro and in vivo. However,few data are available concerning gene transfer intohematopoietic stem cells. In order to explore theefficiency ...Liposomes have several advantages over viral vectorsfor gene delivery both in vitro and in vivo. However,few data are available concerning gene transfer intohematopoietic stem cells. In order to explore theefficiency and the stability of expression of gene transferinto hematopoietic stem cells, we have transduced twomarker genes (Neo^R and Lac Z) co-transfer into humanbone marrow CD34^+ hematopoietic stem cells展开更多
文摘The solution chemical and optical characteristics of formation of amine-terminated polyamidoamine dendrimer G2.0（NH2-PAMAM G2.0）-Au nanocomposites in the aqueous solution of NH2-PAMAM G2.0 at various mole ratios of Au（Ⅲ） to NH2-PAMAM G2.0 were studied by both UV-visible spectrometry and fluorospectrometry. The NH2-PAMAM G2.0-Au nanocomposites, with a type of structure in which one Au nanoparticle is surrounded by several NH2-PAMAM G2.0 dendrimers, emit strong bluish violet fluorescence, and are uniform, water soluble and biocompatible as well as very stable in frozen conditions. The size of gold nanoparticles in the nanocomposites is about 2.5 nm and decreases with the increase of NH2-PAMAM G2.0 concentration. The NH2-PAMAM G2.0 plays an important role in acting as host or micro-reactor for Au（Ⅲ） before Au（Ⅲ） reduction and acting as dispersant and stabilizer for gold nanoparticles after Au（Ⅲ） reduction. Preliminary experiments of cells staining to human embryonic lung fibroblast cell lines show that the NH2-PAMAM G2.0-Au nanocomposites can be used as optical imaging markers for bioanalyses and medical diagnoses.
文摘Fetal liver tissues obtained from 28 human fetuses with gestation age from 3 to 6 months and fetal bone marrow from 35 human fetuses from 3 to 7 months were observed by immunochemical staining with anti-platelet GPⅡ b / Ⅲa monoclonal antibody and ABC technique. In the fetal liver, megakaryocytes were wholly located among growing fetal liver cells and near foci of hemopoiesis. Some megakaryocytes in the fetal liver were small7890- lymphoid-like megakaryocytes. The size of megakaryocytes both in the fetal liver (14.79 ± 4.52μm) and in the fetal bone marrow (16.08±7.39 μm) was small, which did not vary significantly over the gestation age ranging from 3 to 6 or 7 months. However, the maturation stage of megakaryocytes in the fetal liver shifted to more mature stage with the advancement of gestation, although the maturation stage of megakaryocytes in the fetal bone marrow did not change with the advancement of gestation from 4 to 7 months, the megakaryocyte in the fetal bone marrow was less mature
文摘The functional activity and morphological integrity of the porcine thyroid tissue could be maintained up to 96 hrs with organ culture technique. Tg,T<sub>4</sub> and T<sub>3</sub> showed a almost similar localization and immunohistochemical staining pattern: diffuse staining of follicular lumen （L）, granular staining of follicular border（G）, and foamy staining of follicular border （F）. The significant differences between the thyrotropinstimulated and non-stimulated groups were observed. The peripheral vacuoles of colloid were increased （P【0.05） in the former. Dominant staining patterns were G and F in the former. This simple organ culture system is a useful model for studying the functional aspects such as hormone biosynthesis in thyroid gland.
文摘Objective: The objective of research is to explorethe prospective for direct gene transfer to be applied as aform of gene therapy. Materials and methods: ①Experimental animals: Male BALB/c mice, 0 - 8 weeksold;②Preparations of plasmid: PCI-hIL-2 plasmid isexpression vector for human IL-2 containing cytome-galovirus immediate-early enhance/promoter region andT7 promoer;③Injection of plasmids: Male
文摘To demonstrate that low c-myc expression mightexert the effects on differentiation and survival ofleukemic cells, antisense technique was used. Human 2. 7kb c-myc DNA fragment containing exon l, intron 1 and127nt exon 2 was ligated into retroviral vector pDOR-neoin reverse direction. This recombinant plasmid
文摘To apply gene therapy to breast carcinoma wepropose a model for in vitro gene transfer of the herpessimplex thymidine kinase (HSV-tk) gene using Stkvector-producing cells. In this study the HSV-tk gene wastransferred to mouse mammary carcinoma cell line 4Tlwith the method of infection. The HSV-tk gene in thegenome of transduced cells (4Tl/tk) was demonstratedby the method of DNA-PCR. Using a modified
文摘In recent years, great enthusiasm is given to theresearches of cancer gene therapy, which seems to bea promising novel strategy for cancer treatment.There are several strategies to deliver target genes intobodies. Among them, adenovirus-mediated genetransfection exhibits great advantages including highgene transfer efficiency and high expression level. Butthe optimal route of gene delivery is still uncertain.
基金Supported by the Science & Technology Project of Fujian Province(2010Y0027)
文摘AIM:To observe the protective effects of 13-methyltetradecanoic acid(13-MTD)on the injury of cultured brain microvascular endothelial cells(BMECs)induced by oxygen paradox. METHODS:Two screen filtration methods and enzyme digestion method were used to the primary culture and subculture of the neonatal rat cortex BMECs.
基金Hebei Science and Technology Office Programme (07220401D)Guangdong Science and Technology Office Programme (2006B203010 1020)
文摘In order to investigate the mechanism of action of immunoenhancer, the effects of the traditional Chinese medicine immunopromoter on the quantity and the transformation rates of T lymphocytes in the chicken blood were determined. Total 120 chickens were randomly assigned into three groups. The 1% and the 0.5% of the Chinese medicine immunopromoter were added to the chicken drinking water, respectively. The quantity of T lymphocytes in each group was measured by a-Naphthyl acetate esterase （ANAE） staining. The results showed that the percentages of T lymphocytes of the treatment groups were significantly higher than that of the control group （P〈0.05）, and the percentage of the 1% group significantly higher than that of the 0.5% group （P〈0.05）. In conclusion, the transformation rates of T lymphocytes showed that the Chinese medicine immunopromoter had the significant enhancing effect on the transformation rates of T lymphocytes of the treated chickens. The traditional Chinese medicine immunopromoter had the distinct function to promote the quantity and the transformation rate of T lymphocytes.
文摘Based on the characteristics and reinforcement mechanism of vacuum preloading applied in high way soft foundation, the theoretical calculation method of vacuum preloading was presented. According to the equivalent method derived from sand drain foundation subjected to plane and axisymmetric conditions, the three dimensional problem was transformed into plane strain problem. The modified Komala-Huang model was used to model the rheological behavior and the damage theory was also introduced to consider the effect of damage caused by construction disturbance. Numerical simulations were carried out for a high way test section. The results show that simulations of the viscoelastic-plastic-damage model are in good agreement with the field measurements. It suggests that the calculation method, simplified load and boundary condition used in this study are reasonable.
文摘This paper discussed 378 cases of lung cancer which were identified pathologically in the thoracic department of China Medical University from 1980～1986. The sections were stained with H.E., PAS-ab and Keratin, and compared with sections of 131 cases of lung cancer being confirmed beforehand from 1978～1984. Chinese cases showed epidermoid carcinoma 165 (male 146), adenocarcinoma 136(male 82), small cell carcinoma 29 (male 21), large cell carcinoma 19 (male 15) and the others 29 (male 27); Japanese cases were 59(50), 53(25), 3 (2), 11(10), 3(0) respectively. The cases under 40 years of age were 23(6.1%) in China, 3(2.3%)in Japan. Both of them have similar character of histology. But the incidence of lung cancer in youth is higher in China.
文摘Liposomes have several advantages over viral vectorsfor gene delivery both in vitro and in vivo. However,few data are available concerning gene transfer intohematopoietic stem cells. In order to explore theefficiency and the stability of expression of gene transferinto hematopoietic stem cells, we have transduced twomarker genes (Neo^R and Lac Z) co-transfer into humanbone marrow CD34^+ hematopoietic stem cells
