Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together w...Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together with tissue engineering is increasingly becoming a potential choice of treatment.However,direct transplantation of stem cells without scaffolds has yielded poor clinical outcome.Here we show a strategy of using mouse embryonic stem cells(ESCs)cultured within a silk fibroin(SF)based,three-dimensional scaffold with oriented channels by a directional temperature field freezing technique and lysophilization.We find that the ESCs maintained proliferation and migrated in the scaffolds and the cells migrated fastest along the SF channels.SF scaffolds contributed to ESC differentiation into neural and glial cell like cells and expressions of the neural and glial cell markers MAP2 and GFAP were greatly elevated when retinoic acid was used as an inducing factor.Our results suggest that this approach may offer some hope in the future for spinal cord injury repair using SF scaffolds and ESCs.展开更多
A novel composite was prepared by mixing natural rubber latex(NR)with Bombyx mori silk fibroin(SF).The antibacterial property was tested through the method of covering film on the surface of samples,and the composite ...A novel composite was prepared by mixing natural rubber latex(NR)with Bombyx mori silk fibroin(SF).The antibacterial property was tested through the method of covering film on the surface of samples,and the composite was tested by UV-Vis absorption spectrum,tensile-strength measurements and dynamic mechanical analyzer.The result showed that lacteous composite had absorption in UV spectrum,and the mechanical property of composite film was about 0.8MPa,glass transition temperature reduced to-72.3℃ and flexibility modulus reduced.Furthermore the composite had a good antibacterial effectiveness to Escherichial coli,bacteriostasis rate reached 95.3% and 90.0% after incubation for 24h and 48h.展开更多
基金supported by funds from Huazhong University of Science and Technology,Wuhan,China
文摘Spinal cord injury repair is one of the major challenges in medicine,as it can lead to permanent loss of function of central nervous system and damage to other function of the body.Stem cell transplantation together with tissue engineering is increasingly becoming a potential choice of treatment.However,direct transplantation of stem cells without scaffolds has yielded poor clinical outcome.Here we show a strategy of using mouse embryonic stem cells(ESCs)cultured within a silk fibroin(SF)based,three-dimensional scaffold with oriented channels by a directional temperature field freezing technique and lysophilization.We find that the ESCs maintained proliferation and migrated in the scaffolds and the cells migrated fastest along the SF channels.SF scaffolds contributed to ESC differentiation into neural and glial cell like cells and expressions of the neural and glial cell markers MAP2 and GFAP were greatly elevated when retinoic acid was used as an inducing factor.Our results suggest that this approach may offer some hope in the future for spinal cord injury repair using SF scaffolds and ESCs.
文摘A novel composite was prepared by mixing natural rubber latex(NR)with Bombyx mori silk fibroin(SF).The antibacterial property was tested through the method of covering film on the surface of samples,and the composite was tested by UV-Vis absorption spectrum,tensile-strength measurements and dynamic mechanical analyzer.The result showed that lacteous composite had absorption in UV spectrum,and the mechanical property of composite film was about 0.8MPa,glass transition temperature reduced to-72.3℃ and flexibility modulus reduced.Furthermore the composite had a good antibacterial effectiveness to Escherichial coli,bacteriostasis rate reached 95.3% and 90.0% after incubation for 24h and 48h.
