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基于RhoA/ROCK通路研究复方中药对腹水肉鸡心肌损伤的作用
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作者 康杰 王慧敏 +1 位作者 邓瑞强 段智变 《特产研究》 2025年第3期44-48,54,共6页
为分析RhoA/ROCK信号通路在肉鸡腹水综合征(Ascites syndrome, AS)心肌损伤中的作用及复方中药的防治机制,本研究选取100羽健康的罗斯肉鸡(7日龄)并随机分成5组(每组20只),即基础饲粮饲养对照组,多因素法构建的AS模型组,复方中药高、低... 为分析RhoA/ROCK信号通路在肉鸡腹水综合征(Ascites syndrome, AS)心肌损伤中的作用及复方中药的防治机制,本研究选取100羽健康的罗斯肉鸡(7日龄)并随机分成5组(每组20只),即基础饲粮饲养对照组,多因素法构建的AS模型组,复方中药高、低剂量治疗组以及L-精氨酸治疗组。观察临床症状和剖检变化,并通过HE染色和Masson染色分别观察35日龄肉鸡心脏组织结构变化和胶原纤维沉积;计算肉鸡腹水心脏指数(Ascites heart index, AHI);利用实时荧光定量PCR检测心脏组织MYPT1 mRNA的转录情况,ELISA法检测心脏组织RhoA/ROCK信号通路相关蛋白的表达。结果表明,与空白对照组相比,模型组肉鸡腹部膨大,触压有波动感,腹腔内呈现大量淡黄色液体,右心肥大,心脏组织结构排列紊乱,胶原纤维大量沉积,而且M组AHI显著升高(P <0.01);荧光定量PCR结果表明,心脏组织MYPT1 mRNA转录显著升高(P <0.01)。ELISA结果表明,RhoA、ROCK、P-MLC表达显著升高(P <0.01),而MLCP表达显著降低(P <0.01);与AS组相比,复方中药治疗组的肉鸡相关临床症状及上述指标明显改善。本研究发现,RhoA/ROCK通路介导的MLC磷酸化参与了AS中心肌损伤的发生,复方中药水提液可通过调节RhoA/ROCK抑制心肌损伤来有效防治AS。 展开更多
关键词 复方中药 肉鸡腹水综合征 心肌损伤 RhoA/rock通路
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miR-202-5p通过靶向抑制ROCK1表达减轻急性呼吸窘迫综合征新生大鼠肺损伤
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作者 杨英阁 张晋雷 +2 位作者 孙岩 陈升 梁正 《中国医科大学学报》 北大核心 2025年第9期814-820,共7页
目的 探讨miR-202-5p对急性呼吸窘迫综合征(ARDS)新生大鼠肺损伤的保护作用及其机制。方法 60只新生SD大鼠随机分为对照组(腹腔注射生理盐水)、ARDS组[腹腔注射脂多糖(4 mg/kg)建立ARDS模型]、ARDS+miR-NC组[ARDS大鼠尾静脉注射miR-NC(2... 目的 探讨miR-202-5p对急性呼吸窘迫综合征(ARDS)新生大鼠肺损伤的保护作用及其机制。方法 60只新生SD大鼠随机分为对照组(腹腔注射生理盐水)、ARDS组[腹腔注射脂多糖(4 mg/kg)建立ARDS模型]、ARDS+miR-NC组[ARDS大鼠尾静脉注射miR-NC(2 mg/kg),1次/4 h,连续干预12 h]、ARDS+miR-202-5p组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h]、ARDS+miR-202-5p+ov-NC组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h;脂多糖注射前3 d尾静脉注射6×10~8 PFU过表达对照腺病毒]和ARDS+miR-202-5p+ov-ROCK1组[ARDS大鼠尾静脉注射miR-202-5p(2 mg/kg),1次/4 h,连续干预12 h;脂多糖注射前3 d尾静脉注射6×10^(8) PFU过表达ROCK1腺病毒],每组10只。实时定量PCR检测大鼠肺组织中miR-202-5p和ROCK1 mRNA表达;HE染色观察大鼠肺组织病理损伤;计算大鼠肺组织湿重/干重比值来评价大鼠肺水肿程度;Western blotting检测大鼠肺组织中ROCK1、TLR4、p-NF-κB p65和p-IκBα蛋白表达。双萤光素酶报告基因实验验证miR-202-5p与ROCK1的关系。结果 miR-202-5p在ARDS大鼠肺组织中表达下调,过表达miR-202-5p可减轻ARDS大鼠肺组织病理损伤和肺水肿。ROCK1 mRNA在ARDS大鼠肺组织中表达上调;过表达miR-202-5p降低ARDS大鼠肺组织中ROCK1表达,而过表达ROCK1逆转了过表达miR-202-5p对ARDS新生大鼠肺损伤的保护作用。过表达miR-202-5p抑制ARDS大鼠肺组织中TLR4、p-NF-κB p65和p-IκBα蛋白表达,过表达ROCK1逆转此作用。双萤光素酶报告基因实验结果显示miR-202-5p靶向结合ROCK1 mRNA的3’UTR。结论 miR-202-5p通过靶向抑制ROCK1表达减轻ARDS大鼠肺损伤,其机制可能与抑制ROCK1介导的TLR4/NF-κB信号通路激活有关。 展开更多
关键词 急性呼吸窘迫综合征 miR-202-5p rock1 TLR4/NF-κB信号通路 肺损伤
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毛兰素通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎
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作者 徐可欣 王丹丹 +5 位作者 金泓宇 杜月 李莉 宋艺兰 延光海 李良昌 《中国比较医学杂志》 北大核心 2025年第4期11-20,共10页
目的探究毛兰素(Erianin)在特应性皮炎(atopic dermatitis,AD)中的作用及其在高迁移率族蛋白1(high mobility group box-1,HMGB1)/晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)-Ras同源基因家族成员A(Ra... 目的探究毛兰素(Erianin)在特应性皮炎(atopic dermatitis,AD)中的作用及其在高迁移率族蛋白1(high mobility group box-1,HMGB1)/晚期糖基化终末产物受体(receptor for advanced glycation end products,RAGE)-Ras同源基因家族成员A(Ras homolog gene family member A,RhoA)/Rho关联含卷曲螺旋结合蛋白激酶1(recombinant Rho associated coiled coil containing protein kinase 1,ROCK1)信号通路中的调控机制。方法1-氯-2,4-二硝基苯(1-Chloro-2,4-dinitrobenzene,DNCB)诱导BALB/c小鼠作为AD的模型,测量小鼠的皮肤厚度、脾和淋巴结的重量。甲苯胺蓝和HE染色检测小鼠的背部皮肤和耳朵的病理改变;ELISA检测炎症因子水平;肿瘤坏死因子-α(tumor necrosis factor-α,TNF-α)刺激HaCaT细胞建立AD体外模型;采用流式细胞术检测细胞活性氧(reactive oxygen species,ROS);免疫荧光法检测线粒体活性氧(mitochondrion reactive oxygen species,mtROS);TUNEL检测细胞凋亡情况;免疫蛋白印迹法检测HMGB1、RAGE、RhoA、ROCK1蛋白表达情况。结果在体内实验中毛兰素抑制皮肤厚度的增加,减轻脾和淋巴结重量,改善炎症细胞的浸润和肥大细胞脱颗粒,降低炎症因子水平(P<0.05)。在体外实验中,毛兰素减少TNF-α诱导的HaCaT细胞ROS、mtROS的产生(P<0.01)。毛兰素治疗后HMGB1、RAGE、RhoA及ROCK1的蛋白表达量下降(P<0.01);使用RAGE特异性阻断剂(TFA)处理r-HMGB1刺激的HaCaT细胞后,HMGB1的表达没有发生变化,RAGE、RhoA及ROCK1表达减少(P<0.01);在Rho激酶抑制剂Y-27632+r-HMGB1组中,除RAGE的表达没有降低,其余结果与TFA+r-HMGB1组相近。结论毛兰素可能通过调节HMGB1/RAGE-RhoA/ROCK1信号通路缓解特应性皮炎。 展开更多
关键词 毛兰素 特应性皮炎 HMGB1 RAGE RHOA rock1
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重楼皂苷Ⅱ与反式肉桂醛调控ROCK/LIMK/cofilin通路抑制子宫腺肌病异位内膜细胞侵袭迁移的机制研究
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作者 曹涵 王子璐 +2 位作者 王楚矗 石雅馨 师伟 《海南医科大学学报》 北大核心 2025年第19期1477-1487,共11页
目的:探究重楼皂苷Ⅱ(polyphyllin,PPⅡ)与反式肉桂醛(trans-cinnamaldehyde,TCA)通过调控ROCK/LIMK/cofilin通路抑制子宫腺肌病(adenomyosis,AM)进展的机制。方法:提取人子宫腺肌病异位内膜细胞(adenomyosis-derived cells,AMDC)进行培... 目的:探究重楼皂苷Ⅱ(polyphyllin,PPⅡ)与反式肉桂醛(trans-cinnamaldehyde,TCA)通过调控ROCK/LIMK/cofilin通路抑制子宫腺肌病(adenomyosis,AM)进展的机制。方法:提取人子宫腺肌病异位内膜细胞(adenomyosis-derived cells,AMDC)进行培养,采用CCK-8法检测梯度浓度的重楼皂苷Ⅰ、Ⅱ、Ⅶ(PPⅠ、PPⅡ、PPⅦ)及TCA对AMDC活力的影响;采用正交试验设计筛选PPⅡ与TCA(简称PT)的最佳配比;采用Transwell实验和免疫荧光实验检测PT抑制AMDC侵袭、迁移的能力;采用Western blot检测ROCK1、LIMK、p-LIMK、cofilin、p-cofilin的蛋白表达水平;对ICR小鼠腹腔注射梯度浓度的PT 30 d,采用苏木素-伊红染色法观察小鼠肝、脾和肾组织的形态学改变,采用酶联免疫吸附法检测血清中丙氨酸氨基转移酶(alanine aminotransferase,ALT)、谷草转氨酶(aspartate aminotransferase,AST)、碱性磷酸酶(alkaline phosphatase,ALP)和血尿素氮(blood urea nitrogen,BUN)活性。他莫昔芬滴喂新生ICR雌鼠建立AM模型,设置空白对照组、模型组、PT低剂量组、PT高剂量组和阳性药物复方米非司酮组,药物干预30 d,采用H&E染色检测小鼠子宫的形态学改变;采用免疫组化评估子宫组织内ROCK1、p-LIMK、p-cofilin以及KI67的表达水平改变。结果:体外实验结果显示,PPⅡ和TCA抑制AMDC活力效果较好;PT抑制AMDC侵袭、迁移效果最显著(P<0.001),下调ROCK1、LIMK、p-LIMK、cofilin、p-cofilin蛋白表达(P<0.05),抑制AMDC骨架重排(P<0.001)。体内实验结果表明,PT药物未损伤小鼠肝、脾和肾组织形态,不影响血清ALP、BUN活性和AST/ALT比值,能够减轻AM模型小鼠子宫内膜腺体向肌层侵袭的数量,降低AM小鼠子宫组织中ROCK1、p-LIMK、p-cofilin和KI67的表达水平(P<0.05)。结论:PT可通过调控ROCK/LIMK/cofilin通路抑制AM腺体向肌层的侵袭和迁移,延缓AM进展。 展开更多
关键词 子宫腺肌病 重楼皂苷Ⅱ 反式肉桂醛 rock/LIMK/cofilin通路
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桂皮醛抑制RhoA/ROCK1/MLC通路改善膝骨关节炎小鼠滑膜巨噬细胞胞葬功能
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作者 苏子珊 于利凯 +4 位作者 田地 刘尚齐 王泽恩 王培民 张农山 《中国药理学通报》 北大核心 2025年第9期1636-1643,共8页
目的探讨桂皮醛对膝骨关节炎(knee osteoarthritis,KOA)小鼠滑膜病变的治疗作用,及其对滑膜巨噬细胞胞葬功能的调控机制。方法动物实验中,提取小鼠血清及滑膜组织,HE染色评估滑膜组织炎性细胞浸润;ELISA检测血清中白细胞介素等炎症因子... 目的探讨桂皮醛对膝骨关节炎(knee osteoarthritis,KOA)小鼠滑膜病变的治疗作用,及其对滑膜巨噬细胞胞葬功能的调控机制。方法动物实验中,提取小鼠血清及滑膜组织,HE染色评估滑膜组织炎性细胞浸润;ELISA检测血清中白细胞介素等炎症因子含量;Western blot检测滑膜组织中Ras同系物家族成员A(Ras homolog family member A,RhoA)、Rho激酶1(Rho-associated kinase 1,ROCK1)、肌球蛋白轻链(myosin light chain,MLC)及p-MLC蛋白的表达;RT-qPCR检测滑膜组织中的炎症因子及通路相关mRNA表达;TUNEL染色检测滑膜组织凋亡。细胞实验中,对干预后的RAW267.4细胞使用Western blot及RT-qPCR进行上述指标的检测,最后使用共聚焦显微镜检测胞葬功能。结果桂皮醛干预后,滑膜炎性浸润明显减少,RhoA/ROCK1/MLC信号通路的mRNA及蛋白表达明显下调,TUNEL染色荧光强度明显下降,巨噬细胞胞葬功能提高。结论桂皮醛可以通过抑制RhoA/ROCK1/MLC信号通路,增强巨噬细胞胞葬作用,进而改善滑膜组织炎症反应,延缓KOA小鼠病情进展。 展开更多
关键词 桂皮醛 胞葬 膝骨关节炎 滑膜 巨噬细胞 RhoA/rock1/MLC
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ROCK-siRNA转染对AngⅡ诱导的内皮细胞衰老及内皮细胞微粒的影响
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作者 王恺 王嫣 +3 位作者 陈天琪 彭芳 周慧 史琴 《细胞与分子免疫学杂志》 北大核心 2025年第9期778-783,共6页
目的探讨转染ROCK-siRNA对血管紧张素Ⅱ(AngⅡ)诱导的内皮细胞衰老及内皮细胞微粒(EMP)的影响。方法使用AngⅡ(1.0μmol/L)诱导人脐静脉内皮细胞(HUVEC)制备细胞衰老模型,并用ROCK干扰RNA(ROCK-siRNA)转染细胞,分为对照组、模型组、转... 目的探讨转染ROCK-siRNA对血管紧张素Ⅱ(AngⅡ)诱导的内皮细胞衰老及内皮细胞微粒(EMP)的影响。方法使用AngⅡ(1.0μmol/L)诱导人脐静脉内皮细胞(HUVEC)制备细胞衰老模型,并用ROCK干扰RNA(ROCK-siRNA)转染细胞,分为对照组、模型组、转染阴性对照组(AngⅡ联合NC-siRNA)、ROCK-siRNA转染组(AngⅡ联合ROCK-siRNA)。采用SA-β-Gal法鉴定细胞衰老程度,流式细胞术检测培养液上清中EMP的数量及细胞内活性氧(ROS)水平,Western blot法检测各组细胞中沉默信息调节因子2相关酶1(SIRT1)、p53蛋白的表达。结果ROCK-siRNA转染内皮细胞后,可明显减少AngⅡ诱导的衰老的内皮细胞数量,抑制衰老细胞释放CD31^(+)EMP及ROS生成,同时可上调SIRT1蛋白表达、下调p53蛋白表达水平。结论沉默ROCK可抑制EMP释放,减少ROS生成,调控SIRT1、p53表达,延缓AngⅡ诱导的内皮细胞衰老。 展开更多
关键词 内皮细胞微粒(EMP) 衰老 rock 血管紧张素Ⅱ(AngⅡ)
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青蒿琥酯调节RhoA/ROCK信号通路对高糖诱导的滋养层细胞损伤的影响
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作者 吴晓茜 朱燕 +2 位作者 徐淋 刘艳 李敏 《中国免疫学杂志》 北大核心 2025年第10期2318-2323,共6页
目的:探究青蒿琥酯(ART)对高糖(HG)诱导的滋养层细胞损伤的影响及其作用机制。方法:将人滋养层细胞HTR-8/SVneo分为对照组、HG组、ART低浓度组、ART高浓度组和ART高浓度+溶血磷脂酸(LPA)组。CCK-8法检测细胞活力,流式细胞术检测细胞凋亡... 目的:探究青蒿琥酯(ART)对高糖(HG)诱导的滋养层细胞损伤的影响及其作用机制。方法:将人滋养层细胞HTR-8/SVneo分为对照组、HG组、ART低浓度组、ART高浓度组和ART高浓度+溶血磷脂酸(LPA)组。CCK-8法检测细胞活力,流式细胞术检测细胞凋亡,ELISA检测TNF-α、IL-1β和IL-10水平,试剂盒检测乳酸脱氢酶(LDH)、丙二醛(MDA)和超氧化物歧化酶(SOD)水平,Western blot检测细胞Bax、Bcl-2、Ras同源基因家族成员A(RhoA)、Rho相关卷曲螺旋蛋白激酶(ROCK)1、ROCK2表达。结果:0.4~1.6μmol/L ART处理可增强HTR-8/SVneo细胞活力(P<0.05),ART显著增强HG诱导的HTR-8/SVneo细胞活力,且1.6μmol/L ART处理下细胞活力增强最显著(P<0.05)。与对照组相比,HG组HTR-8/SVneo细胞活力、IL-10、SOD、Bcl-2水平降低,凋亡率、TNF-α、IL-1β、LDH、MDA、Bcl-2、RhoA、ROCK1、ROCK2水平升高(P<0.05);与HG组相比,ART低、高浓度组HTR-8/SVneo细胞活力、IL-10、SOD、Bcl-2水平升高,凋亡率、TNF-α、IL-1β、LDH、MDA、Bcl-2、RhoA、ROCK1、ROCK2水平降低(P<0.05);与ART高浓度组相比,ART高浓度+LPA组HTR-8/SVneo细胞活力、IL-10、SOD、Bcl-2水平降低,凋亡率、TNF-α、IL-1β、LDH、MDA、Bcl-2、RhoA、ROCK1、ROCK2水平升高(P<0.05)。结论:ART能抑制滋养层细胞炎症反应和氧化应激,减少细胞凋亡,其作用机制可能与抑制RhoA/ROCK信号通路有关。 展开更多
关键词 滋养层细胞 高糖 青蒿琥酯 RhoA/rock信号通路
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基于Rho A/ROCK通路探索鬼箭羽醇提物对肝纤维化小鼠的影响
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作者 马敏 曾华华 +2 位作者 孙宇杰 胡嘉敏 黄德斌 《中华中医药学刊》 北大核心 2025年第8期131-136,I0047,共7页
目的基于Rho A/ROCK信号通路探究鬼箭羽醇提物(Ethanolic extracts of euonymus alatus,EEEA)对四氯化碳(CCl_(4))诱导的肝纤维化(Hepatic fibrosis,HF)模型小鼠的影响。方法将50只C57BL/6小鼠随机分为空白组(Normal group,NG)、模型组(... 目的基于Rho A/ROCK信号通路探究鬼箭羽醇提物(Ethanolic extracts of euonymus alatus,EEEA)对四氯化碳(CCl_(4))诱导的肝纤维化(Hepatic fibrosis,HF)模型小鼠的影响。方法将50只C57BL/6小鼠随机分为空白组(Normal group,NG)、模型组(Model group,MG)、鬼箭羽低剂量组(EA-L)、鬼箭羽中剂量组(EA-M)、鬼箭羽高剂量组(EA-H),每组10只。除NG外,其余各组小鼠腹腔注射含有25%CCl_(4)的橄榄油混悬液造模。造模的同时,NG、MG以双蒸水灌胃,其余3组按对应剂量灌胃:EA-L、EA-M、EA-H 3个剂量分别为2、4、8 g/kg(相当于含生药量)。6周后取材,收集小鼠血清和肝组织,采用谷草转氨酶(Glutamic oxalacetic transaminase,AST)、谷丙转氨酶(Glutamate pyruvate transaminase,ALT)测试盒检测血清中肝功能指标。ELISA法检测血清中肿瘤坏死因子-α(Tumor necrosis factor-α,TNF-α)和白细胞介素6(Interleukin-6,IL-6)含量,相同方式检测肝组织羟脯氨酸(Hydroxyproline,Hyp)、转换生长因子β1(Transforming growth factor-β1,TGF-β1)的含量。HE和Masson染色分析小鼠肝脏的损伤和胶原沉积情况。Western blot法检测肝组织中α平滑肌动蛋白(α-smooth muscle actin,α-SMA)、Ⅰ型胶原蛋白(TypeⅠCollagen,CollagenⅠ)以及信号通路Rho A/ROCK相关蛋白的相对表达量。结果与MG相比,EA-M和EA-H血清ALT、AST、TNF-α、IL-6呈下降趋势;与MG相比,鬼箭羽药物组肝组织Hyp、TGF-β1的含量不同程度下降(P<0.05,P<0.01)。鬼箭羽药物组的肝形态结构、细胞炎性浸润、纤维变化及胶原沉积呈剂量依赖性优于MG;与MG相比,EA-L、EA-M和EA-H的α-SMA、CollagenⅠ及Rho A/ROCK信号通路相关蛋白表达水平下降(P<0.05,P<0.01)。与MG相比,EA-L、EA-M和EA-H的α-SMA、CollagenⅠ及Rho A/ROCK信号通路相关蛋白mRNA表达下降(P<0.05,P<0.01)。结论鬼箭羽醇提物具有明显的降酶护肝、抗肝纤维化的作用,而抑制Rho A/ROCK信号通路是其抗肝纤维化的作用机制之一。 展开更多
关键词 鬼箭羽醇提物 肝纤维化 Rho A/rock信号通路
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Experimental and numerical study on mechanical behavior of rock beam fracture under unloading with different thicknesses and spans in deep mining working face
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作者 SUN Xiao-ming JIANG Ming +1 位作者 ZHAO Wen-chao MIAO Cheng-yu 《Journal of Central South University》 2025年第7期2570-2592,共23页
The stability of the roof in coal mining is crucial for ensuring safe extraction.Studying the mechanical behavior of rock beams under various conditions is essential for improving coal mining safety.However,research o... The stability of the roof in coal mining is crucial for ensuring safe extraction.Studying the mechanical behavior of rock beams under various conditions is essential for improving coal mining safety.However,research on the dynamic response of rock beams under sudden unloading remains limited.This study utilized a self-developed bidirectional loading and unilateral unloading test system to simulate how sudden lower strata subsidence induces the fracture of upper hard rock beams.Bottom unloading experiments were performed on rock beams with varying thicknesses and spans.The experiments recorded surface crack development and internal damage evolution using high speed photography and acoustic emission monitoring.The results show that rock beams experience multiple stress reductions after unloading,with the largest reduction occurring in the first stage.Flexural deformation was observed,becoming more pronounced as the thickness-span ratio decreased.Greater thickness increased shear cracks and crack expansion angles,while larger spans promoted tensile cracks,arched crack formation,and notable rock spalling.Acoustic emission analysis showed that signal count and energy increased with thickness and span.Finally,discrete element numerical simulations revealed the critical controlling role of harder rock strata in rock beam failure:when the harder strata are at the top,cracks are sharp,and shear failure is more likely;when they are at the bottom,the overall failure range expands,and cracks tend to form arches.These findings improve the understanding of dynamic rock beam fracture under sudden unloading and offer theoretical guidance for roof stability control in deep mining. 展开更多
关键词 roof rock beam bottom unloading thickness and span tensile crack composite rock beam harder rock strata
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Influence of the saw-tooth angle of rock joints on the shear performance of 2G-NPR bolt
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作者 REN Shu-lin HE Man-chao +3 位作者 YUAN Yong TAO Zhi-gang ZHU Chun YIN Qian 《Journal of Central South University》 2025年第8期2998-3014,共17页
The shear performance of bolts plays a crucial role in controlling rock mass stability,and the roughness of the joint surface is one of the main factors affecting the mechanical properties of anchored joints.The 2nd g... The shear performance of bolts plays a crucial role in controlling rock mass stability,and the roughness of the joint surface is one of the main factors affecting the mechanical properties of anchored joints.The 2nd generation of negative Poisson ratio(2G-NPR)bolt is a new independently developed material characterized by high strength and toughness.However,the influence of joint surface roughness on its anchorage shear performance remains unexplored.This study involves preparing regular saw-tooth jointed rock masses and conducting laboratory shear comparison tests on unbolted samples,2G-NPR bolts,and Q235 steel anchors.A three-dimensional finite element method,developed by the author,was employed for numerical simulations to analyze the influence of saw-tooth angles on the shear resistance of anchored bolts.The findings show that the anchorage of bolts enhances the shear strength and deformation of saw-tooth rock joints.The 2G-NPR bolts demonstrate superior performance in shear strength and deformation enhancement compared to Q235 steel anchors,including improved toughening and crack-arresting effects.Furthermore,the improvement of the shear strength and displacement of the bolt decreases with the increase of the joint saw-tooth angle.These findings provide a valuable test basis for the engineering application of 2G-NPR bolts in rock mass stabilization. 展开更多
关键词 jointed rock mass shear resistance bolt anchorage 2G-NPR bolt saw-tooth rock joint
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Seismic pseudo-static stability analysis of bedding jointed rock slope stability under the interaction of the upper dangerous rock mass and lower stepped slope mass based on acceleration assessment
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作者 LI De-jian XIAO Yu +3 位作者 YANG Yan-song YU Peng-cheng ZHANG Ying-bin ZHAO Lian-heng 《Journal of Central South University》 2025年第7期2619-2637,共19页
In the practical slope engineering,the stability of lower sliding mass(region A)with back tensile cracks of the jointed rock slope attracts more attentions,but the upper rock mass(region B)may also be unstable.Therefo... In the practical slope engineering,the stability of lower sliding mass(region A)with back tensile cracks of the jointed rock slope attracts more attentions,but the upper rock mass(region B)may also be unstable.Therefore,in this study,based on the stepped failure mode of bedding jointed rock slopes,considering the influence of the upper rock mass on the lower stepped sliding mass,the improved failure model for analyzing the interaction force(F_(AB))between two regions is constructed,and the safety factors(F_(S))of two regions and whole region are derived.In addition,this paper proposes a method to determine the existence of F_(AB) using their respective acceleration values(a_(A) and a_(B))when regions A and B are unstable.The influences of key parameters on two regions and the whole region are analyzed.The results show that the variation of the F_(AB) and F_(S) of two regions can be obtained accurately based on the improved failure model.The accuracy of the improved failure model is verified by comparative analysis.The research results can explain the interaction mechanism of two regions and the natural phenomenon of slope failure caused by the development of cracks. 展开更多
关键词 bedding jointed rock slope upper dangerous rock mass stepped sliding interaction mechanism stability analysis
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HMGB1对复发性流产模型子宫内膜容受性及RhoA/ROCK2信号通路的影响
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作者 王娇 李素叶 +4 位作者 焦艳 王磊 崔家璐 冀雪霞 张聪祎 《医学研究与战创伤救治》 北大核心 2025年第6期575-581,共7页
目的探讨高迁移率群盒蛋白1(HMGB1)在复发性流产(RSA)模型中对子宫内膜容受性的影响,以及其与RhoA/ROCK2信号通路之间的关系。方法雌性CBA/J小鼠按照随机数字表法分成5组:Con组(正常妊娠小鼠),RSA组(复发性流产模型小鼠)、sh-HMGB1组(RS... 目的探讨高迁移率群盒蛋白1(HMGB1)在复发性流产(RSA)模型中对子宫内膜容受性的影响,以及其与RhoA/ROCK2信号通路之间的关系。方法雌性CBA/J小鼠按照随机数字表法分成5组:Con组(正常妊娠小鼠),RSA组(复发性流产模型小鼠)、sh-HMGB1组(RSA小鼠体内转染sh-HMGB1组)、sh-HMGB1+LPA组(RSA小鼠体内转染sh-HMGB1组并注射RhoA激活剂溶血磷脂酸)、C3组(RSA小鼠注射Rho A抑制剂C3外酶)。HE染色进行组织病理学分析。ELISA试剂盒检测子宫内膜组织炎症因子水平。检测子宫内膜厚度与腺体数目。TUNEL检测组织细胞凋亡。qRT-PCR和蛋白免疫印迹检测HMGB1、HOXa10、VEGF、Caspase-9、Caspase-3、RhoA和ROCK2的表达水平。结果与Con组相比,RSA组的TNF-α、IFN-γ、IL-6水平、TUNEL阳性细胞减少(P<0.05),RhoA、ROCK2的m RNA和蛋白表达水平显著增加(P<0.05),平均胚胎着床点、子宫内膜厚度、腺体数目数、HOXa10、VEGF、Caspase-9和Caspase-3的mRNA和蛋白表达水平显著降低(P<0.05)。与RSA组相比,sh-HMGB1组和C3组的TNF-α、IFN-γ、IL-6水平、TUNEL阳性细胞减少(P<0.05),RhoA、ROCK2的m RNA和蛋白表达水平显著降低(P<0.05),平均胚胎着床点、子宫内膜厚度、腺体数目数、HOXa10、VEGF、Caspase-9和Caspase-3的m RNA和蛋白表达水平显著增加(P<0.05)。与sh-HMGB1组相比,sh-HMGB1+LPA组TNF-α、IFN-γ、IL-6水平、TUNEL阳性细胞减少(P<0.05),RhoA、ROCK2的m RNA和蛋白表达水平显著增加(P<0.05),平均胚胎着床点、子宫内膜厚度、腺体数目数、HOXa10、VEGF、Caspase-9和Caspase-3的m RNA和蛋白表达水平显著降低(P<0.05)。结论HMGB1在RSA模型中通过促进炎症反应,抑制子宫内膜的细胞凋亡对子宫内膜容受性产生负面影响,其机制可能与Rho A/ROCK2信号通路的异常激活有关。 展开更多
关键词 高迁移率群盒蛋白1 复发性流产 子宫内膜容受性 RhoA/rock2
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Strength and failure characteristics of hard rock containing a single structural plane under varied loading angles : A true triaxial investigation
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作者 XU Huai-sheng LI Shao-jun +3 位作者 XU Ding-ping LIU Xu-feng FENG Guang-liang WANG Zhao-feng 《Journal of Central South University》 2025年第5期1903-1921,共19页
The spatial relationship between structural planes and principal stresses significantly affects the mechanical properties of deep hard rock.This paper examines the effect of the loading angle under true triaxial compr... The spatial relationship between structural planes and principal stresses significantly affects the mechanical properties of deep hard rock.This paper examines the effect of the loading angle under true triaxial compression.While previous studies focused on the angleβbetween the maximum principal stress and the structural plane,the role of angleω,between the intermediate principal stress and the structural plane,is often overlooked.Utilizing artificially prefabricated granite specimens with a single non-penetrating structural plane,we set the loading angleβto range from 0°to 90°across seven groups,and assignedωvalues of 0°and 90°in two separate groups.The results show that the peak strength is negatively correlated withβup to 45°,beyond which it tends to stabilize.The angleωexerts a strengthening effect on the peak strength.Deformation mainly occurs post-peak,with the strain values ε_(1) and ε_(3) reaching levels 2−3 times higher than those in intact rock.The structural plane significantly influences failure mode whenω=0°,while failure localizes near the σ_(3) surface of the specimens whenω=90°.The findings enhance data on structural plane rocks under triaxial compression and inform theoretical research,excavation,and support design of rock structures. 展开更多
关键词 true triaxial compression hard rock structural plane loading angle STRENGTH failure characteristics
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Static compressive mechanical properties and disturbed state concept-based theoretical model of gypsum rocks with coupled influences of wet-dry cycles and flow rates
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作者 JIANG Song HUANG Ming +2 位作者 WANG Gang XU Chao-shui XIONG Jun 《Journal of Central South University》 2025年第7期2638-2660,共23页
Gypsum rocks are highly susceptible to mechanical deterioration under the coupled effects of wet-dry(W-D)cycles and flow rates,which significantly influence the stability of underground excavations.Despite extensive r... Gypsum rocks are highly susceptible to mechanical deterioration under the coupled effects of wet-dry(W-D)cycles and flow rates,which significantly influence the stability of underground excavations.Despite extensive research on the effects of W-D cycles,the coupling influence of flow rates and W-D cycles on gypsum rocks remains poorly understood.This study investigates the mechanical behavior and deterioration mechanisms of gypsum rocks subjected to varying W-D cycles and flow rate conditions.Axial compression tests,along with nuclear magnetic resonance(NMR)techniques,were employed to analyze the stress-strain response and microstructural changes.Based on the disturbed state concept(DSC)theory,a W-D deterioration model and a DSC-based constitutive model were developed to describe the degradation trends and mechanical responses of gypsum rocks under different conditions.The results demonstrate that key mechanical indices,elastic modulus,cohesion,uniaxial compressive strength(UCS),and internal friction angle,exhibit logarithmic declines with increasing W-D cycles,with higher flow rates accelerating the deterioration process.The theoretical models accurately capture the nonlinear compaction behavior,peak stress,and post-peak response of gypsum specimens.This study provides valuable insights for predicting the mechanical behavior of gypsum rocks and improving the stability assessments of underground structures under complex environmental conditions. 展开更多
关键词 gypsum rocks wet-dry cycles flow rates mechanical properties disturbed state concept
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Acoustic emission response and rupture evolution analysis of triaxial compression damage of hot dry rock under seawater fatigue dissolution
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作者 LI Cun-bao LAN Ling +1 位作者 XIE He-ping HU Jian-jun 《Journal of Central South University》 2025年第8期3035-3056,共22页
Analyzing the fatigue damage characteristics of hot dry rock(HDR)affected by seawater thermal shock cycles is required for the efficient exploitation of HDR and the conservation of freshwater resources.Mechanical and ... Analyzing the fatigue damage characteristics of hot dry rock(HDR)affected by seawater thermal shock cycles is required for the efficient exploitation of HDR and the conservation of freshwater resources.Mechanical and acoustic emission(AE)monitoring tests were conducted during the triaxial compression of HDR at different confining pressures,temperatures,and numbers of seawater thermal shocks to investigate the seawater damage of HDR.The test results indicated an increase in the cumulative AE counts with increasing temperature and number of seawater thermal shocks,and a decrease in AE counts with increasing confining pressure.The effect of the number of seawater thermal shocks was significant.The AE counts were 276% higher at 15 than at 0 seawater thermal shocks.The b-value increased with the number of thermal shocks and stabilized after 5 shocks.Most of the damage was small fractures,which reduced the rock’s damage resistance.The AE time series under HDR triaxial compression exhibited multifractal features.High energy AE events dominated the damage mechanism of HDR,indicating shear damage to the HDR.Therefore,this study can provide a reference for seawater as a heat transfer fluid in the design of geothermal energy resource extraction. 展开更多
关键词 fatigue damage hot dry rock seawater thermal shocks triaxial compression acoustic emission
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A new damage constitutive model for rock strain softening based on an improved Logistic function
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作者 GUO Yun-peng LIU Dong-qiao +1 位作者 YANG Sheng-kai LI Jie-yu 《Journal of Central South University》 2025年第8期3070-3094,共25页
This study proposed a new and more flexible S-shaped rock damage evolution model from a phenomenological perspective based on an improved Logistic function to describe the characteristics of the rock strain softening ... This study proposed a new and more flexible S-shaped rock damage evolution model from a phenomenological perspective based on an improved Logistic function to describe the characteristics of the rock strain softening and damage process.Simultaneously,it established a constitutive model capable of describing the entire process of rock pre-peak compaction and post-peak strain softening deformation,considering the nonlinear effects of the initial compaction stage of rocks,combined with damage mechanics theory and effective medium theory.In addition,this research verified the rationality of the constructed damage constitutive model using results from uniaxial and conventional triaxial compression tests on Miluo granite,yellow sandstone,mudstone,and glutenite.The results indicate that based on the improved Logistic function,the theoretical damage model accurately describes the entire evolution of damage characteristics during rock compression deformation,from maintenance through gradual onset,accelerated development to deceleration and termination,in a simple and unified expression.At the same time,the constructed constitutive model can accurately simulate the stress-strain process of different rock types under uniaxial and conventional triaxial compression,and the theoretical model curve closely aligns with experimental data.Compared to existing constitutive models,the proposed model has significant advantages.The damage model parameters a,r and β have clear physical meanings and interact competitively,where the three parameters collectively determine the shape of the theoretical stress−strain curve. 展开更多
关键词 rock mechanics strain softening improved Logistic function S-shaped model damage evolution constitutive model
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Stress gradient analytic solution and reasonable support prestress of roadway surrounding rock based on unified strength criterion:A case study
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作者 JING Suo-lin WEN Zhi-jie +2 位作者 ZUO Yu-jun LI Qiu-ju HAO Peng 《Journal of Central South University》 2025年第2期449-468,共20页
The stress gradient of surrounding rock and reasonable prestress of support are the keys to ensuring the stability of roadways.The elastic-plastic analytical solution for surrounding rock was derived based on unified ... The stress gradient of surrounding rock and reasonable prestress of support are the keys to ensuring the stability of roadways.The elastic-plastic analytical solution for surrounding rock was derived based on unified strength theory.A model for solving the stress gradient of the surrounding rock with the intermediate principal stress parameter b was established.The correctness and applicability of the solution for the stress gradient in the roadway surrounding rock was verified via multiple methods.Furthermore,the laws of stress,displacement,and the plastic zone of the surrounding rock with different b values and prestresses were revealed.As b increases,the stress gradient in the plastic zone increases,and the displacement and plastic zone radius decrease.As the prestress increases,the peak stress shifts toward the sidewalls,and the stress and stress gradient increments decrease.In addition,the displacement increment and plastic zone increment were proposed to characterize the support effect.The balance point of the plastic zone area appears before that of the displacement zone.The relationship between the stress gradient compensation coefficient and the prestress is obtained.This study provides a research method and idea for determining the reasonable prestress of support in roadways. 展开更多
关键词 PRESTRESS support compensation surrounding rock damage stress gradient analytic solution unified strength theory
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Research on damage characteristics and constitutive model of rock mass under true triaxial cyclic loading based on acoustic emission
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作者 LI Ying-ming FAN Chao-tao +6 位作者 DONG Chun-liang ZHAO Guang-ming MENG Xiang-rui WANG Xiang-jun SHI Wen-qiu WU Xin-wen GAO Jiang-huai 《Journal of Central South University》 2025年第5期1938-1954,共17页
Aiming at the problem of deep surrounding rock instability induced by roadway excavation or mining disturbance,the true triaxial loading system was used to conduct graded cyclic maximum principal stress σ_(1) and int... Aiming at the problem of deep surrounding rock instability induced by roadway excavation or mining disturbance,the true triaxial loading system was used to conduct graded cyclic maximum principal stress σ_(1) and intermediate principal stress σ_(2) tests on sandstone to simulate the effect of mining stress in actual underground engineering.The influences of each principal stress cycle on the mechanical properties,acoustic emission(AE)characteristics,and fracture characteristics of sandstone were analyzed.The damage characteristics of sandstone under true triaxial cyclic loading were studied.Furthermore,the damage constitutive model of rock mass under true triaxial cyclic loading was established based on AE cumulative ringing count.The quantitative investigation was conducted on cumulative-damage changes in circulating sandstone,which elucidated the mechanism of damage deterioration in sandstone subjected to true triaxial cyclic loading.The results show that the influence of the graded cycleσ_(1) on limit maximum principal strain ɛ_(1max) and limit minimum principal strainɛ_(3max) was significantly greater than that of the limit intermediate principal strain ɛ_(2max).Graded cycleσ_(2) had a greater impact onɛ_(2max) and a smaller impact onɛ_(3max).The elasticity modulus of sandstone decreased exponentially with the increased cyclic load amplitude,while the Poisson ratio increased linearly.b of AE showed a trend of increasing,decreasing,slightly fluctuating,and finally decreasing during cyclingσ_(1).b showed a trend of slight fluctuation,large fluctuation,and finally increase during cyclingσ_(2).Sandstone specimens experienced mainly tensile failure,tensile-shear composite failure,and mainly shear failure with increased initialσ_(2) orσ_(3).This was determined by analyzing the rise angle-average frequency of the AE parameter,corresponding to the rock specimens from splitting failure to shear failure.Besides,the mechanical damage behavior of sandstone under true triaxial cyclic loading could be well described by the established constitutive model.At the same time,it was found that the sandstone damage variable decreased with increasedσ_(2) during cyclingσ_(1).The damage variable decreased first and then increased with increasedσ_(3) during cyclingσ_(2). 展开更多
关键词 rock mechanics true triaxial cyclic principal stress acoustic emission constitutive model damage variable
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Anisotropic strength and deformation of irregular columnar jointed rock masses under triaxial stress
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作者 QUE Xiang-cheng ZHU Zhen-de +1 位作者 NIU Zi-hao ZHU Shu 《Journal of Central South University》 2025年第2期643-655,共13页
The special columnar jointed structure endows rocks with significant anisotropy,accurately grasping the strength and deformation properties of a columnar jointed rock mass(CJRM)under complex geological conditions is c... The special columnar jointed structure endows rocks with significant anisotropy,accurately grasping the strength and deformation properties of a columnar jointed rock mass(CJRM)under complex geological conditions is crucial for related engineering safety.Combined with the irregular jointed networks observed in the field,artificial irregular CJRM(ICJRM)samples with various inclination angles were prepared for triaxial tests.The results showed that the increase in confining pressure can enhance the ability of the ICJRM to resist deformation and failure,and reduce the deformation and strength anisotropic degrees.Considering the field stress situation,the engineering parts with an inclination angle of 30°−45°need to be taken seriously.Four typical failure modes were identified,and the sample with an inclination angle of 15°showed the same failure behavior as the field CJRM.Traditional and improved joint factor methods were used to establish empirical relationships for predicting the strength and deformation of CJRM under triaxial stress.Since the improved joint factor method can reflect the unique structure of CJRM,the predictive ability of the empirical relationship based on the improved method is better than that based on the traditional joint factor method. 展开更多
关键词 irregular columnar jointed rock mass triaxial stress STRENGTH DEFORMATION anisotropic mechanical property empirical relation
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高寒草甸有机碳结构和成分组成及Rock-Eval热解研究
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作者 张永利 冉勇 《地球化学》 北大核心 2025年第3期426-435,共10页
近年来对高寒草甸有机碳结构和成分的研究开始增多,但未见将先进13C固态核磁共振(SS-NMR)技术和Rock-Eval热解技术同时应用于高寒草甸有机碳表征的研究。本研究采用化学分离法将高寒草甸样品连续分为原始(OS)级分、去碳酸盐(DC)级分、去... 近年来对高寒草甸有机碳结构和成分的研究开始增多,但未见将先进13C固态核磁共振(SS-NMR)技术和Rock-Eval热解技术同时应用于高寒草甸有机碳表征的研究。本研究采用化学分离法将高寒草甸样品连续分为原始(OS)级分、去碳酸盐(DC)级分、去矿(DM)级分、去类脂(DF)级分和酸不可水解(NHC)级分。元素分析结果显示,OS、DC、DM、DF和NHC级分的有机碳含量分别为7.7%、8.8%、41.6%、38.4%和54.2%,稳定C同位素(δ^(13)C)值分别为−23.7‰、−25.0‰、−25.4‰、−25.1‰和−26.5‰。稳定C、N同位素和贝叶斯混合模型分析结果表明,高寒草甸有机碳的C3植物源和微生物源分别为46.5%和53.5%。13C SS-NMR分析结果表明,高寒草甸有机碳的结构组成中,脂肪碳和芳香碳分别占总有机碳的55.4%和34.5%。其中,脂肪碳主要包括氧烷基碳(O-alkyl C,23.8%)、烷基碳(Alkyl C,19.0%)和甲氧基碳/N-烷基碳(OCH3/NCH,12.6%);芳香碳主要包括质子化芳香碳(Arom C-H,15.6%)和非质子化芳香碳(Arom C-C,13.1%)。生物大分子成分组成分析结果表明,不稳定的碳水化合物和蛋白质是高寒草甸的主要成分,非质子化木炭和具有Alkyl C结构的孢粉素是稳定有机碳的重要成分。Rock-Eval热解分析结果表明,氧指数(OI)与极性碳(Polar C)含量、碳水化合物+蛋白质+木质素、Alkyl C/O-alkyl C值呈显著相关关系(R^(2)≥0.96,P<0.01),说明OI可表征高寒草甸不稳定有机碳的分解潜力和腐殖化状态;S2与稳定生物源的Alkyl C和孢粉素呈显著正相关关系(R^(2)≥0.96,P<0.01),残留有机碳(RC)与非质子化木炭呈显著正相关关系(R^(2)=0.91,P<0.05),表明S2和RC可代表高寒草甸稳定有机碳的结构和成分组成。 展开更多
关键词 脂肪碳 芳香碳 同位素 固态核磁共振(SS-NMR) rock-EVAL
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