Selenium is one of the essential trace elements in human body,however,due to the limitation of geographic factors,the intake of selenium is seriously insufficient in most regions.In this study,selenium-enriched peanut...Selenium is one of the essential trace elements in human body,however,due to the limitation of geographic factors,the intake of selenium is seriously insufficient in most regions.In this study,selenium-enriched peanut sprouts with high selenium content were prepared by soaking peanut seeds in sodium selenite.The content and distribution of selenium in germinated peanuts were investigated.The results showed that 200μmol/L sodium selenite and germination for 6 days resulted in the highest total selenium,organic selenium content,and organic selenium conversion in peanut sprouts.Selenium exists in peanut sprouts mainly in organic selenium form,of which selenoproteins are the most critical organic selenium carriers.ABTS free radical scavenging capacity and reducing power assays showed that alkali-soluble protein had the highest antioxidant activity among the four soluble proteins,attributed to its high selenium binding level.Radicle and cotyledons of peanut seedlings were significantly enriched with selenium compared to hypocotyl.Amino acid analysis and SDS-PAGE results showed that selenium increases significantly after peanut germination and selenium enrichment.This study provides a simple,environmentally friendly,and effective way of selenium enrichment and offers a theoretical basis for applying selenium-enriched foods in food and medicine.展开更多
为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的...为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的生长状况结合对其生物量与硒含量的测定,选育出一株富硒优势啤酒酵母。通过培养条件为添加质量浓度35 mg/L,加硒时间8 h,培养时间36 h,得到的酵母WX-1的生物量提高到(5192±36)mg/L,较原始菌株WX-01提高了201%,硒含量达到(1475±33)μg/g,较原始菌株提高了330%,其有机硒产量和转化率分别为7658μg/L和97.1%。扫描电镜分析酵母菌富集后表面有少量单质硒析出。另外红外光谱在特定区域出现不同强度的吸收峰表明酵母细胞参与了硒蛋白的合成。展开更多
基金supported by the National Natural Science Foundation of China[32172259]Key Research and Development Project of Henan Province[231111111800]+1 种基金Innovative Funds Plan of Henan University of Technology [2021ZKCJ03]The Program for the Top Young Talents of Henan Associate for Science and Technology.
文摘Selenium is one of the essential trace elements in human body,however,due to the limitation of geographic factors,the intake of selenium is seriously insufficient in most regions.In this study,selenium-enriched peanut sprouts with high selenium content were prepared by soaking peanut seeds in sodium selenite.The content and distribution of selenium in germinated peanuts were investigated.The results showed that 200μmol/L sodium selenite and germination for 6 days resulted in the highest total selenium,organic selenium content,and organic selenium conversion in peanut sprouts.Selenium exists in peanut sprouts mainly in organic selenium form,of which selenoproteins are the most critical organic selenium carriers.ABTS free radical scavenging capacity and reducing power assays showed that alkali-soluble protein had the highest antioxidant activity among the four soluble proteins,attributed to its high selenium binding level.Radicle and cotyledons of peanut seedlings were significantly enriched with selenium compared to hypocotyl.Amino acid analysis and SDS-PAGE results showed that selenium increases significantly after peanut germination and selenium enrichment.This study provides a simple,environmentally friendly,and effective way of selenium enrichment and offers a theoretical basis for applying selenium-enriched foods in food and medicine.
文摘为了进一步研究微生物富硒的能力,得到富硒能力较强的啤酒酵母,以啤酒酵母WX-01为出发菌,通过高浓度亚硒酸钠初筛,再经过常压室温等离子体(atmospheric and room temperature plasma,ARTP)诱变处理以及亚硒酸钠抗性平板筛选,观察菌株的生长状况结合对其生物量与硒含量的测定,选育出一株富硒优势啤酒酵母。通过培养条件为添加质量浓度35 mg/L,加硒时间8 h,培养时间36 h,得到的酵母WX-1的生物量提高到(5192±36)mg/L,较原始菌株WX-01提高了201%,硒含量达到(1475±33)μg/g,较原始菌株提高了330%,其有机硒产量和转化率分别为7658μg/L和97.1%。扫描电镜分析酵母菌富集后表面有少量单质硒析出。另外红外光谱在特定区域出现不同强度的吸收峰表明酵母细胞参与了硒蛋白的合成。
