为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、...为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、香茅醛、柠檬醛)进行了定性和定量分析。该方法简便、高效,在25 min内山苍子精油中各成分分离良好。各成分在一定范围内均线性关系良好(R2>0.999),精密度、稳定性及重复性均符合要求,平均加标回收率为93.40%~107.35%。结果表明:该方法符合方法学验证要求,可用于山苍子精油的多成分含量测定,并为其质量评价提供依据。展开更多
A new method was developed for the analysis of the volatile and semi-volatile acidic components in tobacco by gas chromatography-mass spectrometry selected ion monitoring(GC-MS,SIM)method.The acidic components in toba...A new method was developed for the analysis of the volatile and semi-volatile acidic components in tobacco by gas chromatography-mass spectrometry selected ion monitoring(GC-MS,SIM)method.The acidic components in tobacco samples were extracted by methylene chloride using simultaneous distillation and extraction equipment,and they were analyzed by HP-INNOWAX column(30m×250μm×0.25μm).Thirteen acidic components were quantitatively determined by internal standard curve method.The experiment results showed that the added standard recoveries of the acidic components were in the range from 80.6% to 98.8%,the relative standard deviations(RSD)were less than 2.0% and the correlation coefficients were more than 0.99.The method is simple,rapid and accurate for the determination of the volatile and semi-volatile acidic components in tobacco.展开更多
The organic acids in Hawthorn fruits from Henan Huixian and Tongbai Mountain were determined by gas chromatography-mass spectrometry method and 23 organic acids were identified.The main organic acids in Hawthorn fruit...The organic acids in Hawthorn fruits from Henan Huixian and Tongbai Mountain were determined by gas chromatography-mass spectrometry method and 23 organic acids were identified.The main organic acids in Hawthorn fruit were linoleic acid,oleic acid,linolenic acid,Citric acid,and hexadecanoic acid.There are almost the same kinds of organic acids in the two samples,but the contents of the organic acids in the two samples were very different.The contents of 8 main organic acids in Hawthorn fruits were determined by GC-MS selected ion monitoring(SIM)using internal standard curve method.The added standard recovery of the method was in the range of 92.8～103.2%,the relative standard deviations(RSD)were less than 3.5%,the correlation coefficients were more than 0.999 and the detection limits were in the range of 1.0～2.5 mg/L.展开更多
文摘为了探究山苍子精油中主要成分的绝对含量,建立了一种气相色谱-质谱/选择离子扫描(gas chromatography mass spectrometry/selective ion monitoring,GC-MS/SIM)方法,对其中主要成分(α-蒎烯、β-蒎烯、D-柠檬烯、1,8-桉叶素、芳樟醇、香茅醛、柠檬醛)进行了定性和定量分析。该方法简便、高效,在25 min内山苍子精油中各成分分离良好。各成分在一定范围内均线性关系良好(R2>0.999),精密度、稳定性及重复性均符合要求,平均加标回收率为93.40%~107.35%。结果表明:该方法符合方法学验证要求,可用于山苍子精油的多成分含量测定,并为其质量评价提供依据。
文摘A new method was developed for the analysis of the volatile and semi-volatile acidic components in tobacco by gas chromatography-mass spectrometry selected ion monitoring(GC-MS,SIM)method.The acidic components in tobacco samples were extracted by methylene chloride using simultaneous distillation and extraction equipment,and they were analyzed by HP-INNOWAX column(30m×250μm×0.25μm).Thirteen acidic components were quantitatively determined by internal standard curve method.The experiment results showed that the added standard recoveries of the acidic components were in the range from 80.6% to 98.8%,the relative standard deviations(RSD)were less than 2.0% and the correlation coefficients were more than 0.99.The method is simple,rapid and accurate for the determination of the volatile and semi-volatile acidic components in tobacco.
文摘The organic acids in Hawthorn fruits from Henan Huixian and Tongbai Mountain were determined by gas chromatography-mass spectrometry method and 23 organic acids were identified.The main organic acids in Hawthorn fruit were linoleic acid,oleic acid,linolenic acid,Citric acid,and hexadecanoic acid.There are almost the same kinds of organic acids in the two samples,but the contents of the organic acids in the two samples were very different.The contents of 8 main organic acids in Hawthorn fruits were determined by GC-MS selected ion monitoring(SIM)using internal standard curve method.The added standard recovery of the method was in the range of 92.8～103.2%,the relative standard deviations(RSD)were less than 3.5%,the correlation coefficients were more than 0.999 and the detection limits were in the range of 1.0～2.5 mg/L.
