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SUMO特异性蛋白酶1在铁死亡中的潜在作用
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作者 谢滨 白蒙 +3 位作者 吴妍 沃璐璐 黄莺 张晶 《上海交通大学学报(医学版)》 北大核心 2025年第1期11-19,共9页
目的:探究SUMO特异性蛋白酶1(SUMO-specific peptidase 1,SENP1)在铁死亡中的潜在作用。方法:通过肿瘤基因组图谱(The Cancer Genome Atlas,TCGA)数据库分析SENP1基因与铁死亡相关基因酰基辅酶A合成酶长链家族成员4(acyl-CoA synthetase... 目的:探究SUMO特异性蛋白酶1(SUMO-specific peptidase 1,SENP1)在铁死亡中的潜在作用。方法:通过肿瘤基因组图谱(The Cancer Genome Atlas,TCGA)数据库分析SENP1基因与铁死亡相关基因酰基辅酶A合成酶长链家族成员4(acyl-CoA synthetase long chain family member 4,ACSL4)和谷胱甘肽过氧化物酶4(glutathione peroxidase 4,GPX4)表达的相关性。使用铁死亡诱导剂RSL3(RAS-selective lethal 3)诱导人纤维素瘤细胞HT1080、鼠纤维素瘤细胞MCA-205和人胚胎肾细胞293T发生铁死亡,通过实时荧光定量PCR(quantitative real-time PCR,RT-qPCR)和Western blotting检测SENP1的表达。在293T细胞中通过免疫沉淀-质谱联用技术检测SENP1在铁死亡过程中的相互作用蛋白。在293T细胞中瞬转Flag-SENP1过表达质粒,并通过RT-qPCR和Western blotting实验检测SENP1的过表达效率以及铁死亡相关基因ACSL4和GPX4的表达水平。结果:TCGA数据库分析结果显示,在绝大多数的肿瘤组织中,SENP1和ACSL4的表达呈正相关,与GPX4的表达呈负相关。RT-qPCR和Western blotting结果显示,RSL3处理HT1080、MCA-205和293T细胞一段时间后,SENP1的表达水平均发生明显下调。免疫沉淀-质谱联用技术鉴定结果显示,SENP1在细胞铁死亡过程中富集SUMO分子。Western blotting结果显示,SENP1过表达后ACSL4蛋白水平上升,GPX4蛋白水平无明显变化;RT-qPCR结果显示,SENP1过表达后,ACSL4和GPX4 mRNA水平均无明显变化。结论:SENP1基因表达在铁死亡过程中发生下调,并可能参与调控铁死亡相关蛋白ACSL4的稳定性。 展开更多
关键词 铁死亡 sumo特异性蛋白酶1 酰基辅酶A合成酶长链家族成员4 谷胱甘肽过氧化物酶4 小分子泛素相关修饰物蛋白
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基于Plant Simulation的双离合器装配线仿真优化 被引量:1
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作者 江涛 刘雪梅 《农业装备与车辆工程》 2025年第6期97-102,共6页
在工程项目制定后,通过搭建仿真模型,对项目方案进行分析评估、优化与改进,有助于解决实际工程项目可能出现的问题,减少人力物力浪费,提高优化效率。以某企业DC300双离合器装配线为研究对象,结合装配工艺流程,利用仿真软件Plant Simulat... 在工程项目制定后,通过搭建仿真模型,对项目方案进行分析评估、优化与改进,有助于解决实际工程项目可能出现的问题,减少人力物力浪费,提高优化效率。以某企业DC300双离合器装配线为研究对象,结合装配工艺流程,利用仿真软件Plant Simulation构建装配线仿真模型,并进行装配线运行过程仿真。通过对生产线节拍、设备利用率等相关数据进行分析评估,找出生产线的瓶颈工位,通过工艺结构调整,实现了生产线节拍的优化与改善,达到了生产要求指标。同时进行了多组仿真实验,完成了托盘数量的优化。 展开更多
关键词 Plant simulation 双离合器 装配线
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基于Plant Simulation的产线车辆调度问题
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作者 刘浩然 刘松凯 陈斌 《科学技术与工程》 北大核心 2025年第6期2406-2418,共13页
随着“中国制造2025计划”的进行,军工工业要推行产线无人化,而自动引导车(automated guided vehicle,AGV)作为全自动化生产线的主要物流载体,其调度的优劣直接决定了整个产线的产能和效率。由于军工场所对于安全性的要求,无法采用无线... 随着“中国制造2025计划”的进行,军工工业要推行产线无人化,而自动引导车(automated guided vehicle,AGV)作为全自动化生产线的主要物流载体,其调度的优劣直接决定了整个产线的产能和效率。由于军工场所对于安全性的要求,无法采用无线通信等手段,只能采用点对点的光通讯方式,这也使得AGV通讯的实时性变差。基于Plant Simulation软件,建立了仿真系统模型,打通了该物流仿真软件与现场控制器的实时数据交互通道,实现了仿真系统与现实同步运行,完成了物流仿真软件与现场控制器的无缝连接,有效地解决了军工工业没有无线造成AGV调度实时性差的难题。实验证明,这种方法有效地简化了调度系统的编写难度,并使系统整体的实时性能提高了0.058 s。与传统方法相比,编写时间缩短了9.7倍,调试时间更缩短了22倍。为军工产线实现全自动化奠定基础,并为在危险场所使用脉动生产线提供技术支持。 展开更多
关键词 AGV调度 Plant simulation 离散仿真 实时性 智能调度
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基于Plant Simulation仿真技术的装配生产线优化研究 被引量:1
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作者 崔俊杰 马臻 郭海青 《南方农机》 2025年第2期145-149,共5页
【目的】优化装备生产线,缩短产品交付周期。【方法】基于Plant Simulation仿真技术,对装配生产线进行建模、编程、仿真、分析和优化,有效计算产品产量和成本,识别并优化装配生产线的瓶颈工位。【结果】优化后的三维产线仿真模型产量增... 【目的】优化装备生产线,缩短产品交付周期。【方法】基于Plant Simulation仿真技术,对装配生产线进行建模、编程、仿真、分析和优化,有效计算产品产量和成本,识别并优化装配生产线的瓶颈工位。【结果】优化后的三维产线仿真模型产量增幅接近10%,生产效率明显提升。【结论】通过将智能制造技能竞赛和科研教学活动相结合,能够凝练总结竞赛内容,促使教师紧盯前沿知识,创新改革教学内容,实现以赛促教、以赛促学、以赛促改、以赛促建的多重目标。 展开更多
关键词 Plant simulation仿真技术 生产优化 瓶颈工位
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黄颡鱼去SUMO化修饰关键基因senp8启动子的功能分析及其与脂质代谢的关系
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作者 段芙萱 郝志伟 +4 位作者 刘涛 宋长春 张天华 仲崇超 谭肖英 《中国生物化学与分子生物学报》 北大核心 2025年第4期607-616,I0007,I0008,共12页
该文以黄颡鱼为研究对象,采用双荧光素酶报告实验和凝胶阻滞实验对启动子活性进行分析,同时结合活体养殖实验,探讨去SUMO化修饰关键基因senp8启动子的功能及其与脂质代谢的关系。结果显示,2045 bp序列的senp 8启动子序列上有SP1、TATA-... 该文以黄颡鱼为研究对象,采用双荧光素酶报告实验和凝胶阻滞实验对启动子活性进行分析,同时结合活体养殖实验,探讨去SUMO化修饰关键基因senp8启动子的功能及其与脂质代谢的关系。结果显示,2045 bp序列的senp 8启动子序列上有SP1、TATA-Box、CCAAT-Box、SREBP1、PPARα和PPARγ等关键转录因子结合位点;发现senp8启动子中SREBP1(-901/-910 bp)、PPARα(-1291/-1308 bp)和PPARγ(-1292/-1306 bp)的结合位点正向调控其活性,且油酸或棕榈酸促进这种结合。同时,高脂饲养促进黄颡鱼肝中senp8基因及其蛋白质表达,油酸或棕榈酸处理显著增强了senp8启动子的活性,且这种增强效应可以通过SREBP1、PPARα和PPARγ这些响应元件的调控作用来实现。此外,高脂饲喂能够影响黄颡鱼肝中去SUMO化修饰相关基因的mRNA和蛋白质表达水平。本研究为理解去SUMO化修饰与脂质代谢调控的关系提供新的见解。 展开更多
关键词 黄颡鱼 sumo化修饰 启动子 脂质代谢 转录调控
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Graded density impactor design via machine learning and numerical simulation:Achieve controllable stress and strain rate 被引量:1
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作者 Yahui Huang Ruizhi Zhang +6 位作者 Shuaixiong Liu Jian Peng Yong Liu Han Chen Jian Zhang Guoqiang Luo Qiang Shen 《Defence Technology(防务技术)》 2025年第9期262-273,共12页
The graded density impactor(GDI)dynamic loading technique is crucial for acquiring the dynamic physical property parameters of materials used in weapons.The accuracy and timeliness of GDI structural design are key to ... The graded density impactor(GDI)dynamic loading technique is crucial for acquiring the dynamic physical property parameters of materials used in weapons.The accuracy and timeliness of GDI structural design are key to achieving controllable stress-strain rate loading.In this study,we have,for the first time,combined one-dimensional fluid computational software with machine learning methods.We first elucidated the mechanisms by which GDI structures control stress and strain rates.Subsequently,we constructed a machine learning model to create a structure-property response surface.The results show that altering the loading velocity and interlayer thickness has a pronounced regulatory effect on stress and strain rates.In contrast,the impedance distribution index and target thickness have less significant effects on stress regulation,although there is a matching relationship between target thickness and interlayer thickness.Compared with traditional design methods,the machine learning approach offers a10^(4)—10^(5)times increase in efficiency and the potential to achieve a global optimum,holding promise for guiding the design of GDI. 展开更多
关键词 Machine learning Numerical simulation Graded density impactor Controllable stress-strain rate loading Response surface methodology
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Molecular simulation study of the microstructures and properties of pyridinium ionic liquid[HPy][BF_(4)]mixed with acetonitrile
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作者 XU Jian-Qiang MA Zhao-Peng +2 位作者 CHENG Si LIU Zhi-Cong ZHU Guang-Lai 《原子与分子物理学报》 CAS 北大核心 2025年第4期27-32,共6页
The microstructures and thermodynamic properties of mixed systems comprising pyridinium ionic liquid[HPy][BF_(4)]and acetonitrile at different mole fractions were studied using molecular dynamics simulation in this wo... The microstructures and thermodynamic properties of mixed systems comprising pyridinium ionic liquid[HPy][BF_(4)]and acetonitrile at different mole fractions were studied using molecular dynamics simulation in this work.The following properties were determined:density,self-diffusion coefficient,excess molar volume,and radial distribution function.The results show that with an increase in the mole fraction of[HPy][BF_(4)],the self-diffusion coefficient decreases.Additionally,the excess molar volume initially decreases,reaches a minimum,and then increases.The rules of radial distribution functions(RDFs)of characteristic atoms are different.With increasing the mole fraction of[HPy][BF_(4)],the first peak of the RDFs of HA1-F decreases,while that of CT6-CT6 rises at first and then decreases.This indicates that the solvent molecules affect the polar and non-polar regions of[HPy][BF_(4)]differently. 展开更多
关键词 Pyridinium ionic liquids Thermodynamic properties Molecular dynamics simulation Radial distribution functions
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Simulation and Thermal Runaway Experiment Study on Immersion and Cold Plate Cooling with 4680 Cylindrical Battery Pack
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作者 Wan Fulai Zhao Qingliang +2 位作者 Luo Yitao Zhang Feng Li Guangjun 《汽车技术》 北大核心 2025年第10期10-20,共11页
This study focuses on the thermal management of 4680-type cylindrical lithium-ion battery packs utilizing NCM811 chemistry.It establishes coupled multi-physics models for both immersion and serpentine cold plate cooli... This study focuses on the thermal management of 4680-type cylindrical lithium-ion battery packs utilizing NCM811 chemistry.It establishes coupled multi-physics models for both immersion and serpentine cold plate cooling systems.Through a combination of numerical simulation and experimental validation,the technical advantages and mechanisms of immersion cooling are systematically explored.Simulation results indicate that under a 3C fast-charging condition(inlet temperature 20℃,flow rate 36 L/min),the immersion cooling structure 3demonstrates a triple enhancement in thermal performance compared to the cold plate structure 1:a 13.06%reduction in peak temperature,a 31.67%decrease in overall maximum temperature difference,and a 47.62%decrease in single-cell temperature deviation,while also reducing flow resistance by 33.61%.Furthermore,based on the immersion cooling model,a small battery module comprising seven cylindrical cells was designed for thermal runaway testing via nail penetration.The results show that the peak temperature of the triggered cell was limited to 437.6℃,with a controllable temperature rise gradient of only 3.35℃/s and a rapid cooling rate of 0.6℃/s.The maximum temperature rise of adjacent cells was just 64.8℃,effectively inhibiting thermal propagation.Post-test disassembly revealed that the non-triggered cells retained>99.2%of their original voltage and>99%structural integrity,confirming the module’s ability to achieve“localized failure with global stability.” 展开更多
关键词 Immersion cooling 4680 battery Thermal runaway Numerical simulation
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Multidisciplinary and multi-fidelity coupling methods in aircraft engine simulations
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作者 YANG Xin XIE Pengfu +2 位作者 DONG Xuezhi HE Ai TAN Chunqing 《推进技术》 北大核心 2025年第5期1-12,共12页
To address the limitations of existing coupling methods in aero-engine system simulation,which fail to adaptively adjust iterative parameters and coupling relationships,which can result in low efficiency and in⁃stabil... To address the limitations of existing coupling methods in aero-engine system simulation,which fail to adaptively adjust iterative parameters and coupling relationships,which can result in low efficiency and in⁃stability,this study introduces a‘Dynamic Event-Driven Co-Simulation’algorithm integrated with decision tree algorithms.This algorithm separates the overall coupling relationships and the main solver from the primary mod⁃el,utilizing a dynamic event monitoring module to adaptively adjust simulation strategies,including iteration pa⁃rameters,coupling relationships,and convergence criteria.This facilitates efficient adaptive simulations of dy⁃namic events while balancing solution accuracy and computational efficiency.The research focuses on a twinshaft turbofan engine,establishing six system-level models that encompass overall performance and various sub⁃systems based on three coupling methods,along with a multidisciplinary multi-fidelity simulation framework in⁃corporating a 3D CFD nozzle model.The study tests both model exchange and coupled simulation methods under a 14 s transient acceleration and deceleration scenario.In a 100%throttle condition,a high-fidelity nozzle model is used to analyze the sensitivity of different convergence criteria on computational efficiency and accuracy.Re⁃sults indicate that the accuracy and efficiency achieved with this method are comparable to those of PROOSIS soft⁃ware(18 s and 35 s,respectively),while being 71%more efficient than Simulink software(62 s and 120 s,re⁃spectively).Furthermore,appropriately relaxing the convergence criteria for the 0D model(from 10-6 to 10-4)while enhancing those for the 3D model(from 3000 steps to 6000 steps)can effectively balance computational accuracy and efficiency. 展开更多
关键词 AERO-ENGINE Multi-fidelity simulation Overall performance CO-simulation Integrated model Zooming strategy
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Numerical Simulation of Microstructure Refinement of Al-Cu-Mg-Ag Alloy During Solidification
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作者 ZHU Shiqing ZHANG Hong CHEN Linghao 《材料科学与工程学报》 北大核心 2025年第4期513-519,524,共8页
Al-Cu-Mg-Ag alloys have become a research hotspot because of its good heat resistance.Its excellent mechanical properties are inseparable from the regulation of the structure by researchers.The method of material stru... Al-Cu-Mg-Ag alloys have become a research hotspot because of its good heat resistance.Its excellent mechanical properties are inseparable from the regulation of the structure by researchers.The method of material structure simulation has become more and more perfect.This study employs numerical simulation to investigate the microstructure evolution of Al-Cu-Mg-Ag alloys during solidification with the aim of controlling its structure.The size distribution of Ti-containing particles in an Al-Ti-B master alloy was characterized via microstructure observation,serving as a basis for optimizing the nucleation density parameters for particles of varying radii in the phase field model.The addition of refiner inhibited the growth of dendrites and no longer produced coarse dendrites.With the increase of refiner,the grains gradually tended to form cellular morphology.The refined grains were about 100μm in size.Experimental validation of the simulated as-cast grain morphology was conducted.The samples were observed by metallographic microscope and scanning electron microscope.The addition of refiner had a significant effect on the refinement of the alloy,and the average grain size after refinement was also about 100μm.At the same time,the XRD phase identification of the alloy was carried out.The observation of the microstructure morphology under the scanning electron microscope showed that the precipitated phase was mainly concentrated on the grain boundary.The Al_(2)Cu accounted for about 5%,and the matrix phase FCC accounted for about 95%,which also corresponded well with the simulation results. 展开更多
关键词 Al-Cu-Mg-Ag alloy Phase field simulation Dendrite refinement Phase diagram calculation
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An Algorithm for Cloud-based Web Service Combination Optimization Through Plant Growth Simulation
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作者 Li Qiang Qin Huawei +1 位作者 Qiao Bingqin Wu Ruifang 《系统仿真学报》 北大核心 2025年第2期462-473,共12页
In order to improve the efficiency of cloud-based web services,an improved plant growth simulation algorithm scheduling model.This model first used mathematical methods to describe the relationships between cloud-base... In order to improve the efficiency of cloud-based web services,an improved plant growth simulation algorithm scheduling model.This model first used mathematical methods to describe the relationships between cloud-based web services and the constraints of system resources.Then,a light-induced plant growth simulation algorithm was established.The performance of the algorithm was compared through several plant types,and the best plant model was selected as the setting for the system.Experimental results show that when the number of test cloud-based web services reaches 2048,the model being 2.14 times faster than PSO,2.8 times faster than the ant colony algorithm,2.9 times faster than the bee colony algorithm,and a remarkable 8.38 times faster than the genetic algorithm. 展开更多
关键词 cloud-based service scheduling algorithm resource constraint load optimization cloud computing plant growth simulation algorithm
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Rapid simulation and phase distortion evaluation of thermal blooming effect in internal laser propagation channels
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作者 WU Dong-yu LI Xiang +4 位作者 LI Jia-sheng GAO Liang SONG Yan-song WANG Si DONG Ke-yan 《中国光学(中英文)》 北大核心 2025年第3期520-534,共15页
During the propagation of high-power lasers within internal channels,the laser beam heats the propagation medium,causing the thermal blooming effect that degrades the beam quality at the output.The intricate configura... During the propagation of high-power lasers within internal channels,the laser beam heats the propagation medium,causing the thermal blooming effect that degrades the beam quality at the output.The intricate configuration of the optical path within the internal channel necessitates complex and time-consuming efforts to assess the impact of thermal blooming effect on the optical path.To meet the engineering need for rapid evaluation of thermal blooming effect in optical paths,this study proposed a rapid simulation method for the thermal blooming effect in internal optical paths based on the finite element method.This method discretized the fluid region into infinitesimal elements and employed finite element method for flow field analysis.A simplified analytical model of the flow field region in complex internal channels was established,and regions with similar thermal blooming effect were divided within this model.Based on the calculated optical path differences within these regions,numerical simulations of phase distortion caused by thermal blooming were conducted.The calculated result were compared with those obtained using the existing methods.The findings reveal that for complex optical paths,the discrepancy between the two approaches is less than 3.6%,with similar phase distortion patterns observed.For L-type units,this method and the existing methods identify the same primary factors influencing aberrations and exhibit consistent trends in their variation.This method was used to analyze the impact of thermal blooming effect in a straight channel under different gravity directions.The results show that phase distortion varies with changes in the direction of gravity,and the magnitude of the phase difference is strongly correlated with the component of gravity perpendicular to the optical axis.Compared to the existing methods,this approach offers greater flexibility,obviates the need for complex custom analysis programming.The analytical results of this method enable a rapid assessment of the thermal blooming effect in optical paths within the internal channel.This is especially useful during the engineering design.These results also provide crucial references for developing strategies to suppress thermal blooming effect. 展开更多
关键词 high-power laser thermal blooming effect beam phase numerical simulation thermal coupling effect beam control system
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A hierarchical simulation framework incorporating full-link physical response for short-range infrared detection
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作者 Mingze Gao Lixin Xu +4 位作者 Shiyuan Hu Xiaolong Shi Jiaming Gao Yanjiang Wu Huimin Chen 《Defence Technology(防务技术)》 2025年第8期351-363,共13页
Missile-borne short-range infrared detection(SIRD)technology is commonly used in military ground target detection.In complex battlefield environments,achieving precise strike on ground target is a challenging task.How... Missile-borne short-range infrared detection(SIRD)technology is commonly used in military ground target detection.In complex battlefield environments,achieving precise strike on ground target is a challenging task.However,real battlefield data is limited,and equivalent experiments are costly.Currently,there is a lack of comprehensive physical modeling and numerical simulation methods for SIRD.To this end,this study proposes a SIRD simulation framework incorporating full-link physical response,which is integrated through the radiative transfer layer,the sensor response layer,and the model-driven layer.In the radiative transfer layer,a coupled dynamic detection model is established to describe the external optical channel response of the SIRD system by combining the infrared radiation model and the geometric measurement model.In the sensor response layer,considering photoelectric conversion and signal processing,the internal signal response model of the SIRD system is established by a hybrid mode of parametric modeling and analog circuit analysis.In the model-driven layer,a cosimulation application based on a three-dimensional virtual environment is proposed to drive the full-link physical model,and a parallel ray tracing method is employed for real-time synchronous simulation.The proposed simulation framework can provide pixel-level signal output and is verified by the measured data.The evaluation results of the root mean square error(RMSE)and the Pearson correlation coefficient(PCC)show that the simulated data and the measured data achieve good consistency,and the evaluation results of the waveform eigenvalues indicate that the simulated signals exhibit low errors compared to the measured signals.The proposed simulation framework has the potential to acquire large sample datasets of SIRD under various complex battlefield environments and can provide an effective data source for SIRD application research. 展开更多
关键词 Short-range infrared detection Full-link physical response Signal level simulation
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An improved efficient adaptive method for large-scale multiexplosives explosion simulations
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作者 Tao Li Cheng Wang Baojun Shi 《Defence Technology(防务技术)》 2025年第3期28-47,共20页
Shock wave caused by a sudden release of high-energy,such as explosion and blast,usually affects a significant range of areas.The utilization of a uniform fine mesh to capture sharp shock wave and to obtain precise re... Shock wave caused by a sudden release of high-energy,such as explosion and blast,usually affects a significant range of areas.The utilization of a uniform fine mesh to capture sharp shock wave and to obtain precise results is inefficient in terms of computational resource.This is particularly evident when large-scale fluid field simulations are conducted with significant differences in computational domain size.In this work,a variable-domain-size adaptive mesh enlargement(vAME)method is developed based on the proposed adaptive mesh enlargement(AME)method for modeling multi-explosives explosion problems.The vAME method reduces the division of numerous empty areas or unnecessary computational domains by adaptively suspending enlargement operation in one or two directions,rather than in all directions as in AME method.A series of numerical tests via AME and vAME with varying nonintegral enlargement ratios and different mesh numbers are simulated to verify the efficiency and order of accuracy.An estimate of speedup ratio is analyzed for further efficiency comparison.Several large-scale near-ground explosion experiments with single/multiple explosives are performed to analyze the shock wave superposition formed by the incident wave,reflected wave,and Mach wave.Additionally,the vAME method is employed to validate the accuracy,as well as to investigate the performance of the fluid field and shock wave propagation,considering explosive quantities ranging from 1 to 5 while maintaining a constant total mass.The results show a satisfactory correlation between the overpressure versus time curves for experiments and numerical simulations.The vAME method yields a competitive efficiency,increasing the computational speed to 3.0 and approximately 120,000 times in comparison to AME and the fully fine mesh method,respectively.It indicates that the vAME method reduces the computational cost with minimal impact on the results for such large-scale high-energy release problems with significant differences in computational domain size. 展开更多
关键词 Large-scale explosion Shock wave Adaptive method Fluid field simulations Efficient method
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UAF-based integration of design and simulation model for system-of-systems
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作者 FENG Yimin GE Ping +2 位作者 SHAO Yanli ZOU Qiang LIU Yusheng 《Journal of Systems Engineering and Electronics》 2025年第1期108-126,共19页
Model-based system-of-systems(SOS)engineering(MBSoSE)is becoming a promising solution for the design of SoS with increasing complexity.However,bridging the models from the design phase to the simulation phase poses si... Model-based system-of-systems(SOS)engineering(MBSoSE)is becoming a promising solution for the design of SoS with increasing complexity.However,bridging the models from the design phase to the simulation phase poses significant challenges and requires an integrated approach.In this study,a unified requirement modeling approach is proposed based on unified architecture framework(UAF).Theoretical models are proposed which compose formalized descriptions from both topdown and bottom-up perspectives.Based on the description,the UAF profile is proposed to represent the SoS mission and constituent systems(CS)goal.Moreover,the agent-based simulation information is also described based on the overview,design concepts,and details(ODD)protocol as the complement part of the SoS profile,which can be transformed into different simulation platforms based on the eXtensible markup language(XML)technology and model-to-text method.In this way,the design of the SoS is simulated automatically in the early design stage.Finally,the method is implemented and an example is given to illustrate the whole process. 展开更多
关键词 model-based systems engineering unified architecture framework(UAF) system-of-systems engineering model transformation simulation
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利用SUMO融合系统高效表达可溶性重组蛋白的研究 被引量:22
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作者 姜媛媛 尹成凯 +3 位作者 李晋南 任桂萍 张薇 李德山 《东北农业大学学报》 CAS CSCD 2008年第10期57-62,共6页
利用pSUMO表达载体在大肠杆菌中高效可溶性表达若干种外源蛋白,如鼠源成纤维细胞生长因子(FGF)-21、人源FGF-21、人源白细胞介素(IL)-1β,并与pET-30a(+)及pTYB11表达系统作比较。将鼠源FGF-21、人源FGF-21及人源IL-1β基因分别亚克隆至... 利用pSUMO表达载体在大肠杆菌中高效可溶性表达若干种外源蛋白,如鼠源成纤维细胞生长因子(FGF)-21、人源FGF-21、人源白细胞介素(IL)-1β,并与pET-30a(+)及pTYB11表达系统作比较。将鼠源FGF-21、人源FGF-21及人源IL-1β基因分别亚克隆至pSUMO表达载体上,在大肠杆菌Rosetta(DE3)中诱导表达,并用镍离子螯合柱(Ni-NTA)纯化重组蛋白,透析后利用SUMO蛋白酶I切割融合蛋白,获得纯度较高的成熟蛋白。上述基因在pET-30a(+)及pTYB11中表达量极低,考马斯亮蓝染色几乎检测不到。在pSUMO表达体系中这些基因均以可溶形式表达,且可被SUMO蛋白酶I有效的切割,获得纯度较高的成熟蛋白。上述蛋白可在pSUMO表达系统中获得高效可溶性表达。 展开更多
关键词 sumo sumo蛋白酶 分子伴侣 pTYB11 pET-30a(+) 表达
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Novel optimization techniques for underwater wireless optical communication links:using Monte Carlo simulation
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作者 Intesar Ramley Hamdah MAlzayed +2 位作者 Yas Al-Hadeethi Abeer ZBarasheed Mingguang Chen 《Defence Technology(防务技术)》 2025年第8期386-411,共26页
The Underwater Communication Link(UCL)is a crucial component of Underwater Wireless Optical Communication(UWOC)systems,requiring optimised design to mitigate the high power attenuation inherent in seawater.To ensure t... The Underwater Communication Link(UCL)is a crucial component of Underwater Wireless Optical Communication(UWOC)systems,requiring optimised design to mitigate the high power attenuation inherent in seawater.To ensure the reliability of an optimal UCL design,it is essential to account for the three primary scattering regimes:forward scattering(FSC),backward scattering(BSC),and isotropic scattering(ISC)in seawater channels.This study introduces a new photon-tracking model based on a discrete equation,facilitating Monte Carlo Simulation(MCS)to evaluate how different scattering regimes influence received photon distribution.Three distinct Scattering Regime Contribution Weight(SRCW)probability sets were employed,each representing different UCL operational configurations dominated by specific scattering regimes.The proposed modeling approach enables a comprehensive assessment of the temporal characteristics of received optical pulses,channel loss,and time spread-ultimately defining the optimal UCL design parameters.The key findings of this study include:(1)Enhancing the FSC regime dominance leads to a quasi-light waveguide effect over link spans and small Fields of View(FOV)<25°,significantly improving channel performance in Harbor seawater compared to Coastal seawater.(2)A well-designed UCL with a small FOV(<25°)can minimise channel loss and time spread,ensuring high capacity and efficient performance in both Coastal and Harbor seawaters.(3)When BSC and ISC contributions exceed FSC dominance,the received optical pulse undergoes significant temporal broadening,particularly for larger FOV angles(>25°)and extended link spans.(4)The developed novel MCS-based discrete equation provides a simple yet robust model for simulating photon propagation in both homogeneous and inhomogeneous underwater channels.These insights contribute to developing more efficient and reliable UCL designs with military standards by enhancing UWOC system performance over a longer linkspan for a given limited optical power across various underwater environments. 展开更多
关键词 Channel gain/loss Channel impulse response(CIR) Central limit theorem(CLT) Channel time spread Field of view(FOV) Monte Carlo simulation(MCS) Underwater wireless optical communication(UWOC)
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SUMO融合系统高效表达可溶性鼠源FGF-21及其活性的研究 被引量:7
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作者 任桂萍 姜媛媛 +3 位作者 刘铭瑶 孙国鹏 叶贤龙 李德山 《东北农业大学学报》 CAS CSCD 北大核心 2009年第5期62-67,共6页
利用SUMO表达载体在大肠杆菌中高效可溶性表达鼠源成纤维细胞生长因子(FGF-21)成熟蛋白,并研究其调节血糖的功能。将鼠源FGF-21成熟蛋白基因亚克隆至SUMO表达载体上,在大肠杆菌Rosetta(DE3)中诱导表达,并用镍离子螯合柱(Ni-NTA)纯化重... 利用SUMO表达载体在大肠杆菌中高效可溶性表达鼠源成纤维细胞生长因子(FGF-21)成熟蛋白,并研究其调节血糖的功能。将鼠源FGF-21成熟蛋白基因亚克隆至SUMO表达载体上,在大肠杆菌Rosetta(DE3)中诱导表达,并用镍离子螯合柱(Ni-NTA)纯化重组蛋白,透析后利用SUMO蛋白酶I切割融合蛋白,获得纯度较高的成熟蛋白。将3T3-L1成纤维细胞分化成脂肪细胞,经微量化的GOD-POD法检测培养基中葡萄糖含量,统计学分析葡萄糖消耗率。结果表明,在SUMO表达体系中鼠源FGF-21成熟蛋白基因以可溶形式表达,且可被SUMO蛋白酶I有效地切割,获得纯度较高的成熟蛋白。与未经处理的脂肪细胞对照组相比,经鼠源FGF-21成熟蛋白处理后脂肪细胞对葡萄糖的摄取利用显著增加,残存在培养基中的葡萄糖含量明显减少;两样本比较的t检验,P<0.001,差异极显著。 展开更多
关键词 sumo sumo蛋白酶I 分子伴侣 鼠源FGF-21 葡萄糖吸收
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SUMO在转录中的抑制作用 被引量:12
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作者 李淑晶 程智逵 伍会健 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2007年第7期525-530,共6页
许多调控基因转录的重要蛋白质能被SUMO(small ubiquitin-related modifier)化修饰,这些蛋白质包括转录因子,转录辅助因子和染色质修饰酶.SUMO化修饰对底物蛋白的活性产生影响,在大多数情况下,与转录活性的抑制有关.最近,对SUMO化调控... 许多调控基因转录的重要蛋白质能被SUMO(small ubiquitin-related modifier)化修饰,这些蛋白质包括转录因子,转录辅助因子和染色质修饰酶.SUMO化修饰对底物蛋白的活性产生影响,在大多数情况下,与转录活性的抑制有关.最近,对SUMO化调控转录的机制有了新的认识,认为SUMO化的一个重要作用是促进转录因子与转录抑制因子之间的相互作用.另一方面,已经发现转录共抑制因子HDAC(组蛋白去乙酰化酶)可以作为SUMO化的底物、效应因子和调控因子,说明乙酰化和SUMO化之间复杂的相互作用对基因转录调控起着非常重要的作用. 展开更多
关键词 sumo 转录 抑制 组蛋白去乙酰化酶
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基于SUMO的概念语义相似度研究 被引量:56
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作者 徐德智 郑春卉 K. Passi 《计算机应用》 CSCD 北大核心 2006年第1期180-183,共4页
SUMO(建议上层共享知识本体)是由IEEE标准上层知识本体工作小组所建置的,其目的是发展标准的上层知识本体,这将促进数据互通性、信息搜寻和检索、自动推理和自然语言处理。基于该共享知识本体,提出了一种计算两概念语义相似度的方法。... SUMO(建议上层共享知识本体)是由IEEE标准上层知识本体工作小组所建置的,其目的是发展标准的上层知识本体,这将促进数据互通性、信息搜寻和检索、自动推理和自然语言处理。基于该共享知识本体,提出了一种计算两概念语义相似度的方法。根据该方法实现了一个计算程序模块,并将计算结果同人类的主观判断进行了比较,验证了该方法的有效性。该研究工作可以在面向W eb的知识检索领域中得到应用,还可以为本体的相关研究提供一定的理论基础。 展开更多
关键词 sumo 语义距离 语义相似度 主观判断
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