Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or C...Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or Cu polluted soils.Phytoextraction of heavy metal(HM)using tolerant plants along with organic chelators has gained global attention,and this study provided further insights into this issue.Pot experiments were performed to evaluate the effects of different types of chelators[ethylenediamine tetraacetic acid(EDTA),ethylenediamine disuccinic acid(EDDS)and citric acid(CA)]to improve the phytoextraction capacity of Ricinus communis L.for the metals Cd and Cu.Contaminated soil from a copper smelter was used in this study.A rhizon soil sampler was used to determine the metal concentrations in soil pore water.The results indicated that R.communis was an adequate candidate for chelator induced phytoextraction under the experimental conditions and that EDDS would be a good candidate chelator for the phytoextraction of Cu in soils.EDTA addition obviously improved the uptake of Cd and Cu in R.communis;however,it posed the greatest risk because the concentration of HMs in soil pore water was very high even after 40 days.Compared with EDTA and EDDS,CA had few effects on Cd or Cu uptake in R.communis.Linear relationships between the metal uptake in R.communis shoots and the maximum HM concentrations in soil pore water under HM,2.5,5,and 10 mmol·kg^(-1) treatments were typically observed.From the results of this study,it could be concluded that EDDS treatments played a promising role in increasing the uptake of Cd or Cu and reducing its phytotoxicity.EDDS application could be an effective approach for the phytoextraction of Cd or Cu from polluted soils by growing Ricinus communis L.展开更多
在蓖麻(Ricinus communis L.)中克隆了PLC2N基因,运用生物信息学技术对PLC2N基因编码的蛋白质进行分析,以揭示其在蓖麻生长发育过程中的功能。结果表明,通过克隆蓖麻PLC2N基因,得到1785 bp的片段。PLC2N基因编码的蛋白质分子质量为67.98...在蓖麻(Ricinus communis L.)中克隆了PLC2N基因,运用生物信息学技术对PLC2N基因编码的蛋白质进行分析,以揭示其在蓖麻生长发育过程中的功能。结果表明,通过克隆蓖麻PLC2N基因,得到1785 bp的片段。PLC2N基因编码的蛋白质分子质量为67.98839 ku,等电点为6.16,有594个氨基酸残基,高度亲水,无跨膜区;二级结构中α-螺旋结构在蛋白质序列中占28.11%,共计167种氨基酸;延长链占19.02%,包含113种氨基酸;β转角结构占6.57%,含有39种氨基酸;无规则卷曲部分占总氨基酸数量的46.30%,氨基酸数量约275种。三级结构预测结果显示,该蛋白质包含了α-螺旋、β-转角以及无规则卷曲等关键结构,其中α-螺旋在蛋白质中占据核心地位。经同源性分析,发现蓖麻中的PLC2N基因与陆地棉这一物种之间存在较接近的亲缘关系。展开更多
2018—2020年连续3年以蓖麻品种(Ricinus communis L.)滇蓖麻2号为试材,对蓖麻主茎叶片节位与其节间性状、叶片性状和质量性状等指标进行回归分析,为蓖麻合理采叶提供理论依据。结果显示,①相关系数分析,蓖麻主茎叶片不同着生节位与主...2018—2020年连续3年以蓖麻品种(Ricinus communis L.)滇蓖麻2号为试材,对蓖麻主茎叶片节位与其节间性状、叶片性状和质量性状等指标进行回归分析,为蓖麻合理采叶提供理论依据。结果显示,①相关系数分析,蓖麻主茎叶片不同着生节位与主茎节位长度、叶片长度、叶片宽度、叶柄长度、叶柄宽横径、叶基腺长度、叶片质量和叶片净质量之间均呈极显著正相关,与主茎节间厚横径和叶柄厚横径之间呈显著正相关,与每千克叶片数量之间呈极显著负相关,与其他指标之间呈不显著正相关。②方差分析,蓖麻主茎叶片着生节位与主茎节位长度、叶片长度、叶片宽度和叶柄长度等指标之间均存在极显著差异,与主茎节间厚横径、叶基腺长度和叶片净质量之间均存在显著差异,与其他指标之间差异不显著。③蓖麻主茎叶片着生节位与主茎性状、叶片性状和质量性状之间拟合的回归方程都符合二次曲线规律;由数据拟合得到,主茎叶片着生最好节位为9.20节时,拟合方程中叶片净质量达89.15 g。蓖麻主茎上着生节位对主茎叶片质量的影响表现为,在一定节位范围内,叶片在蓖麻主茎上着生节位越高,叶片长度、叶片宽度、叶柄长度、叶柄宽横径、叶柄基腺长度、叶片质量和叶片净质量等指标均极显著增加,到第9节位的叶片净质量最大,为采叶时合理采叶提供参考。展开更多
基金Supported by the Joint Funds of the National Natural Science Foundation of China(U2340219)。
文摘Cadmium(Cd)or excess copper(Cu)has a great impact in terms of toxicity on living organisms as it severely affects crop growth,yield and food security;thus,warranting appropriate measures for the remediation of Cd or Cu polluted soils.Phytoextraction of heavy metal(HM)using tolerant plants along with organic chelators has gained global attention,and this study provided further insights into this issue.Pot experiments were performed to evaluate the effects of different types of chelators[ethylenediamine tetraacetic acid(EDTA),ethylenediamine disuccinic acid(EDDS)and citric acid(CA)]to improve the phytoextraction capacity of Ricinus communis L.for the metals Cd and Cu.Contaminated soil from a copper smelter was used in this study.A rhizon soil sampler was used to determine the metal concentrations in soil pore water.The results indicated that R.communis was an adequate candidate for chelator induced phytoextraction under the experimental conditions and that EDDS would be a good candidate chelator for the phytoextraction of Cu in soils.EDTA addition obviously improved the uptake of Cd and Cu in R.communis;however,it posed the greatest risk because the concentration of HMs in soil pore water was very high even after 40 days.Compared with EDTA and EDDS,CA had few effects on Cd or Cu uptake in R.communis.Linear relationships between the metal uptake in R.communis shoots and the maximum HM concentrations in soil pore water under HM,2.5,5,and 10 mmol·kg^(-1) treatments were typically observed.From the results of this study,it could be concluded that EDDS treatments played a promising role in increasing the uptake of Cd or Cu and reducing its phytotoxicity.EDDS application could be an effective approach for the phytoextraction of Cd or Cu from polluted soils by growing Ricinus communis L.
文摘在蓖麻(Ricinus communis L.)中克隆了PLC2N基因,运用生物信息学技术对PLC2N基因编码的蛋白质进行分析,以揭示其在蓖麻生长发育过程中的功能。结果表明,通过克隆蓖麻PLC2N基因,得到1785 bp的片段。PLC2N基因编码的蛋白质分子质量为67.98839 ku,等电点为6.16,有594个氨基酸残基,高度亲水,无跨膜区;二级结构中α-螺旋结构在蛋白质序列中占28.11%,共计167种氨基酸;延长链占19.02%,包含113种氨基酸;β转角结构占6.57%,含有39种氨基酸;无规则卷曲部分占总氨基酸数量的46.30%,氨基酸数量约275种。三级结构预测结果显示,该蛋白质包含了α-螺旋、β-转角以及无规则卷曲等关键结构,其中α-螺旋在蛋白质中占据核心地位。经同源性分析,发现蓖麻中的PLC2N基因与陆地棉这一物种之间存在较接近的亲缘关系。
文摘2018—2020年连续3年以蓖麻品种(Ricinus communis L.)滇蓖麻2号为试材,对蓖麻主茎叶片节位与其节间性状、叶片性状和质量性状等指标进行回归分析,为蓖麻合理采叶提供理论依据。结果显示,①相关系数分析,蓖麻主茎叶片不同着生节位与主茎节位长度、叶片长度、叶片宽度、叶柄长度、叶柄宽横径、叶基腺长度、叶片质量和叶片净质量之间均呈极显著正相关,与主茎节间厚横径和叶柄厚横径之间呈显著正相关,与每千克叶片数量之间呈极显著负相关,与其他指标之间呈不显著正相关。②方差分析,蓖麻主茎叶片着生节位与主茎节位长度、叶片长度、叶片宽度和叶柄长度等指标之间均存在极显著差异,与主茎节间厚横径、叶基腺长度和叶片净质量之间均存在显著差异,与其他指标之间差异不显著。③蓖麻主茎叶片着生节位与主茎性状、叶片性状和质量性状之间拟合的回归方程都符合二次曲线规律;由数据拟合得到,主茎叶片着生最好节位为9.20节时,拟合方程中叶片净质量达89.15 g。蓖麻主茎上着生节位对主茎叶片质量的影响表现为,在一定节位范围内,叶片在蓖麻主茎上着生节位越高,叶片长度、叶片宽度、叶柄长度、叶柄宽横径、叶柄基腺长度、叶片质量和叶片净质量等指标均极显著增加,到第9节位的叶片净质量最大,为采叶时合理采叶提供参考。
