OBJECTIVE To explore the new indications and key mechanism of Bazi Bushen capsule(BZBS)by network pharmacology and in vitro experiment.METHODS The potential tar⁃get profiles of the components of BZBS were pre⁃dicted.S...OBJECTIVE To explore the new indications and key mechanism of Bazi Bushen capsule(BZBS)by network pharmacology and in vitro experiment.METHODS The potential tar⁃get profiles of the components of BZBS were pre⁃dicted.Subsequently,new indications for BZBS were predicted by disease ontology(DO)enrich⁃ment analysis and initially validated by GO and KEGG pathway enrichment analysis.Further⁃more,the therapeutic target of BZBS acting on AD signaling pathway were identified by intersec⁃tion analysis.Two Alzheimer′s disease(AD)cell models,BV-2 and SH-SY5Y,were used to pre⁃liminarily verify the anti-AD efficacy and mecha⁃nism of BZBS in vitro.RESULTS In total,1499 non-repeated ingredients were obtained from 16 herbs in BZBS formula,and 1320 BZBS targets with high confidence were predicted.Disease enrichment results strongly suggested that BZBS formula has the potential to be used in the treat⁃ment of AD.In vitro experiments showed that BZ⁃BS could significantly reduce the release of TNF-αand IL-6 and the expression of COX-2 and PSEN1 in Aβ25-35-induced BV-2 cells.BZBS reduced the apoptosis rate of Aβ25-35 induced SH-SY5Y cells,significantly increased mitochon⁃drial membrane potential,reduced the expres⁃sion of Caspase3 active fragment and PSEN1,and increased the expression of IDE.CONCLU⁃SIONS BZBS formula has a potential use in the treatment of AD,which is achieved through regu⁃lation of ERK1/2,NF-κB signaling pathways,and GSK-3β/β-catenin signaling pathway.Further⁃more,the network pharmacology technology is a feasible drug repurposing strategy to reposition new clinical use of approved TCM and explore the mechanism of action.The study lays a foun⁃dation for the subsequent in-depth study of BZBS in the treatment of AD and provides a basis for its application in the clinical treatment of AD.展开更多
在井下斜坡道无人驾驶卡车往往存在因信号传输困难、道路倾斜且缺乏有效特征信息等问题而导致难以稳定高精定位,严重影响井下无人矿卡安全高效作业。为解决上述问题,提出一种基于激光SLAM的井下斜坡道无人矿卡定位与建图算法GFRMINE-LIO...在井下斜坡道无人驾驶卡车往往存在因信号传输困难、道路倾斜且缺乏有效特征信息等问题而导致难以稳定高精定位,严重影响井下无人矿卡安全高效作业。为解决上述问题,提出一种基于激光SLAM的井下斜坡道无人矿卡定位与建图算法GFRMINE-LIO,首先,针对井下斜坡道口两侧均为光滑水泥墙壁,特征点稀少问题,设计了一种基于人工路标的辅助增强定位方法,有效增加特征点云数量,从而优化位姿估计结果,避免建图过程中出现漂移现象;其次,提出融合坡度与曲率信息的SCSA(Slope and Curvature based Segmentation Algorithm)算法,通过分析激光雷达采集的点云数据中的几何特征,精确计算每个点的坡度角和曲率值,有效识别井下倾斜坑洼路面,确保在复杂环境中实现更精确的点云过滤,显著提升算法在复杂地形中的鲁棒性和精度;最后,在已构建地图的基础上利用GICP算法对实时采集的点云数据进行配准,融合GFRMINE-LIO算法修正点云畸变,从而实现高效重定位,相较于原算法定位精度大幅提升。实验结果表明:此算法能够在恶劣环境下更稳定、更快速地实现高精度定位。实际应用表明:在中钢集团山东某井下斜坡道的现场,与原算法相比,该算法精度提升2.90%,Z轴误差降低20.8%,地图质量明显提高,定位精度和鲁棒性均有显著提升,能有效解决井下无人驾驶建图及定位的难题。展开更多
基金Chinese Academy of Engi⁃neering Strategic Consulting Project(2022-XY-45)S&T Program of Hebei(22372502D)+1 种基金Scien⁃tific Research Project of Hebei Provincial Admin⁃istration of Traditional Chinese Medicine(023172)and Scientific Research Project of Hebei Provincial Administration of Traditional Chinese Medicine(2021273)。
文摘OBJECTIVE To explore the new indications and key mechanism of Bazi Bushen capsule(BZBS)by network pharmacology and in vitro experiment.METHODS The potential tar⁃get profiles of the components of BZBS were pre⁃dicted.Subsequently,new indications for BZBS were predicted by disease ontology(DO)enrich⁃ment analysis and initially validated by GO and KEGG pathway enrichment analysis.Further⁃more,the therapeutic target of BZBS acting on AD signaling pathway were identified by intersec⁃tion analysis.Two Alzheimer′s disease(AD)cell models,BV-2 and SH-SY5Y,were used to pre⁃liminarily verify the anti-AD efficacy and mecha⁃nism of BZBS in vitro.RESULTS In total,1499 non-repeated ingredients were obtained from 16 herbs in BZBS formula,and 1320 BZBS targets with high confidence were predicted.Disease enrichment results strongly suggested that BZBS formula has the potential to be used in the treat⁃ment of AD.In vitro experiments showed that BZ⁃BS could significantly reduce the release of TNF-αand IL-6 and the expression of COX-2 and PSEN1 in Aβ25-35-induced BV-2 cells.BZBS reduced the apoptosis rate of Aβ25-35 induced SH-SY5Y cells,significantly increased mitochon⁃drial membrane potential,reduced the expres⁃sion of Caspase3 active fragment and PSEN1,and increased the expression of IDE.CONCLU⁃SIONS BZBS formula has a potential use in the treatment of AD,which is achieved through regu⁃lation of ERK1/2,NF-κB signaling pathways,and GSK-3β/β-catenin signaling pathway.Further⁃more,the network pharmacology technology is a feasible drug repurposing strategy to reposition new clinical use of approved TCM and explore the mechanism of action.The study lays a foun⁃dation for the subsequent in-depth study of BZBS in the treatment of AD and provides a basis for its application in the clinical treatment of AD.
文摘在井下斜坡道无人驾驶卡车往往存在因信号传输困难、道路倾斜且缺乏有效特征信息等问题而导致难以稳定高精定位,严重影响井下无人矿卡安全高效作业。为解决上述问题,提出一种基于激光SLAM的井下斜坡道无人矿卡定位与建图算法GFRMINE-LIO,首先,针对井下斜坡道口两侧均为光滑水泥墙壁,特征点稀少问题,设计了一种基于人工路标的辅助增强定位方法,有效增加特征点云数量,从而优化位姿估计结果,避免建图过程中出现漂移现象;其次,提出融合坡度与曲率信息的SCSA(Slope and Curvature based Segmentation Algorithm)算法,通过分析激光雷达采集的点云数据中的几何特征,精确计算每个点的坡度角和曲率值,有效识别井下倾斜坑洼路面,确保在复杂环境中实现更精确的点云过滤,显著提升算法在复杂地形中的鲁棒性和精度;最后,在已构建地图的基础上利用GICP算法对实时采集的点云数据进行配准,融合GFRMINE-LIO算法修正点云畸变,从而实现高效重定位,相较于原算法定位精度大幅提升。实验结果表明:此算法能够在恶劣环境下更稳定、更快速地实现高精度定位。实际应用表明:在中钢集团山东某井下斜坡道的现场,与原算法相比,该算法精度提升2.90%,Z轴误差降低20.8%,地图质量明显提高,定位精度和鲁棒性均有显著提升,能有效解决井下无人驾驶建图及定位的难题。
