A Review Resistin, a new hormone found in the year 2001 and secreted by adipocytes, is related to type 2 diabetes and obesity. It brings some hope to solve the medical hamper of insulin resistance. The resistin discov...A Review Resistin, a new hormone found in the year 2001 and secreted by adipocytes, is related to type 2 diabetes and obesity. It brings some hope to solve the medical hamper of insulin resistance. The resistin discovery, molecule structure, function and expression, secretion regulation as well as gene polymorphism are reviewed in the article. [展开更多
OBJECTIVE To explore the role of resistin in lung adenocarcinoma progression and its mechanism.METHODS The effect of resistin on A549 cells proliferation was detected by MTS assay.Wound-healing and transwell assays we...OBJECTIVE To explore the role of resistin in lung adenocarcinoma progression and its mechanism.METHODS The effect of resistin on A549 cells proliferation was detected by MTS assay.Wound-healing and transwell assays were used to evaluate the influence of resistin on A549 migration and invasion.Protein expression was detected by western blot.NF-k B translocation was evaluated by immunofluorescence.The expression of resistin in tumor tissue was assayed by immunohisto-chemical staining.RESULTS Compared with para-carcinoma tissues,resistin was overexpressed in tumor tissues.Resistin didn′t significantly affect A549 proliferation,but induced migration and invasion of A549.TLR4was the functional receptor of resistin in A549 cells,and resistin can bind to the second domain of TLR4.Resistin could increase p-EGFR by TLR4,induce PI3K/Akt phosphorylation and NF-k B translocation to nuclear.High resistin expression in lung adenocarcinoma tissues was correlated significantly with metastasis.Resistin was an independent predictor of overall survival.CONCLUSION Resistin promoted A549 migration and invasion by TLR4/EGFR/NF-k B pathway.Resistin was an independent prognosis predictor of lung adenocarcinoma.展开更多
目的初步分析绝经后女性与骨量丢失和低骨密度(bone mineral density,BMD)相关的血清脂肪因子谱。方法选取2022年1月至2023年6月在医院就诊的64例骨量丢失(腰椎、股骨颈、全髋关节或33%桡骨T评分<-1.0)的绝经后女性为病例组。另外,...目的初步分析绝经后女性与骨量丢失和低骨密度(bone mineral density,BMD)相关的血清脂肪因子谱。方法选取2022年1月至2023年6月在医院就诊的64例骨量丢失(腰椎、股骨颈、全髋关节或33%桡骨T评分<-1.0)的绝经后女性为病例组。另外,纳入同期在医院体检的BMD正常的绝经后妇女为对照组。采用酶联免疫吸附试验法检测血清鸢尾素、瘦素水平,并通过Luminex xMAP技术定量脂联素、抵抗素、降脂素。分析两组受试者血清脂肪因子谱。结果与对照组比较,病例组患者血清鸢尾素水平更低(P<0.001),血清抵抗素水平更高(P<0.001),而瘦素、脂联素和降脂素水平无统计学差异(P>0.05)。经Spearman法分析,病例组患者血清鸢尾素水平与腰椎BMD、腰椎T评分、股骨颈BMD、股骨颈T评分呈显著正相关,与骨转换标志物[1型前胶原氨基末端前肽(type-1 procollagen N-terminal,P1NP)、1型胶原交联羧基末端肽(C-terminalcross linked peptide,CTX)]及甘油三酯(triglycerides,TG)、总胆固醇(total cholesterol,TC)、低密度脂蛋白胆固醇(low-density lipoprotein cholesterol,LDL-C)呈显著负相关(P<0.05);血清抵抗素与腰椎BMD、腰椎T评分、股骨颈BMD、股骨颈T评分呈显著负相关,与P1NP和CTX、TG、TC、LDL-C呈显著正相关(P<0.05)。经受试者工作特征(receiver operating characteristic,ROC)曲线分析,血清鸢尾素和抵抗素识别骨量丢失的曲线下面积(area under the curve,AUC)分别为0.855(95%CI:0.788~0.922)、0.844(95%CI:0.722~0.916),二者联合可使AUC提高至0.930(95%CI:0.883~0.976)。结论在绝经后人群中,骨量丢失女性血清低鸢尾素水平和高抵抗素水平与低BMD值有关,两个指标在识别绝经后女性骨量丢失方面具有良好的特异性,这可能为骨量丢失机制的研究提供潜在的分子。展开更多
文摘A Review Resistin, a new hormone found in the year 2001 and secreted by adipocytes, is related to type 2 diabetes and obesity. It brings some hope to solve the medical hamper of insulin resistance. The resistin discovery, molecule structure, function and expression, secretion regulation as well as gene polymorphism are reviewed in the article. [
基金The project supported by National HighTech R&D Program of China(863 Program)(2012AA02A517)National Natural Science Foundation of China(81373490,81573508,81573463)Hunan Provincial Science and Technology Plan of China(2015TP1043)
文摘OBJECTIVE To explore the role of resistin in lung adenocarcinoma progression and its mechanism.METHODS The effect of resistin on A549 cells proliferation was detected by MTS assay.Wound-healing and transwell assays were used to evaluate the influence of resistin on A549 migration and invasion.Protein expression was detected by western blot.NF-k B translocation was evaluated by immunofluorescence.The expression of resistin in tumor tissue was assayed by immunohisto-chemical staining.RESULTS Compared with para-carcinoma tissues,resistin was overexpressed in tumor tissues.Resistin didn′t significantly affect A549 proliferation,but induced migration and invasion of A549.TLR4was the functional receptor of resistin in A549 cells,and resistin can bind to the second domain of TLR4.Resistin could increase p-EGFR by TLR4,induce PI3K/Akt phosphorylation and NF-k B translocation to nuclear.High resistin expression in lung adenocarcinoma tissues was correlated significantly with metastasis.Resistin was an independent predictor of overall survival.CONCLUSION Resistin promoted A549 migration and invasion by TLR4/EGFR/NF-k B pathway.Resistin was an independent prognosis predictor of lung adenocarcinoma.
