Smooth muscle cells can work properly over a large length range,drastic reorganization of the cytoskeletal structure has to occur,but the mechanism behind the process of cytoskeleton reorganization of smooth muscle is...Smooth muscle cells can work properly over a large length range,drastic reorganization of the cytoskeletal structure has to occur,but the mechanism behind the process of cytoskeleton reorganization of smooth muscle is still poorly understood,especially with respect to unique problem of maintaining force generation and transmission within the confine of an ever-changing cytoskeleton in smooth muscle.Using a cytoskeletal preparation derived from skinned airway smooth muscle,we aimed at separating the contribution of actomyosin interaction and elucidating the cytoskeletal stiffness in smooth muscle.The results indicate that there is a Ca++de-展开更多
Erythrocyte tropomodulin（E-Tmod）is a capping protein at the slow-growing end of the actin filaments and regulates the length of the junctional complex in the erythrocyte membrane skeleton<sup>[1]</sup>.E...Erythrocyte tropomodulin（E-Tmod）is a capping protein at the slow-growing end of the actin filaments and regulates the length of the junctional complex in the erythrocyte membrane skeleton<sup>[1]</sup>.E-Tmod has two alternative promoters,P<sub>E0</sub>,and P<sub>E1</sub>,upstream of exons EO and E1,respectively.They drive the expression of two E-Tmod isoforms,E-Tmod41 and ETmod29,which play different yet coordinated roles in cytoskeleton reorganization<sup>[2]</sup>.This study aimed at investigating the expessions of the two E-Tmod isoforms response to the mechanical stresses,i.e.,shear stress and hydrostatic pressure.A cone-plate flow system and a hydrostatic pressure device were developed.Murine erythroleukemia（MEL）cells were subjected展开更多
基金supported by National Natural Science Foundation of China(11172340)Training Program for Hundreds of Distinguished Leading Scientists of ChongqingSpecialized Research Fund for the Doctoral Program of Higher Education of China(20120191120032)
文摘Smooth muscle cells can work properly over a large length range,drastic reorganization of the cytoskeletal structure has to occur,but the mechanism behind the process of cytoskeleton reorganization of smooth muscle is still poorly understood,especially with respect to unique problem of maintaining force generation and transmission within the confine of an ever-changing cytoskeleton in smooth muscle.Using a cytoskeletal preparation derived from skinned airway smooth muscle,we aimed at separating the contribution of actomyosin interaction and elucidating the cytoskeletal stiffness in smooth muscle.The results indicate that there is a Ca++de-
基金supported by Natural Science Foundation of China(No.31170886)
文摘Erythrocyte tropomodulin（E-Tmod）is a capping protein at the slow-growing end of the actin filaments and regulates the length of the junctional complex in the erythrocyte membrane skeleton<sup>[1]</sup>.E-Tmod has two alternative promoters,P<sub>E0</sub>,and P<sub>E1</sub>,upstream of exons EO and E1,respectively.They drive the expression of two E-Tmod isoforms,E-Tmod41 and ETmod29,which play different yet coordinated roles in cytoskeleton reorganization<sup>[2]</sup>.This study aimed at investigating the expessions of the two E-Tmod isoforms response to the mechanical stresses,i.e.,shear stress and hydrostatic pressure.A cone-plate flow system and a hydrostatic pressure device were developed.Murine erythroleukemia（MEL）cells were subjected
